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Hire Dr. Alex M.

Belgium

USD 60 /hr

Peptide nucleic acids design and modification expert

Profile Summary

Alex Manicardi obtained is his Ph.D. in Chemical Sciences in 2012 at the University of Parma (Italy) under the supervision of Prof. R. Corradini, with a thesis named “Modified peptide nucleic acids (PNAs) for nucleic acids detection and anti-miR activity” for which he obtained an award from C.I.N.M.P.I.S. (Consorzio Interuniversitario Nazionale “Metodologie e Processi Inovativi di Sintesi”) for “Best 2012 Thesis”. During his career he worked at I.S.I.S. (Institute de Science et d’Ingénierie Supramuléculaire – Strasbourg, France) under the supervision of Prof. N. Winssinger as visiting Ph.D. student and UGent (Gent, Belgium) under the supervision of Prof. A. Madder as visiting researcher, where he now hold a FWO [PEGASUS]² Marie Skłodowska-Curie fellowship and become scientific responsible of the project “MeFuran” granted by IOF (Industrial Research Fund of UGent). During the years the main target of the research activity was the design and synthesis of backbone modified PNA systems able to inhibit the microRNA (miR) activity in cellular systems and control biological processes (i.e. cellular differentiation, cell growth, … ). This was done by developing a series of molecules able to efficiently penetrate inside the cytosol without need of transfecting agents or physical methods, for the future application to in vivo systems and for the generation of smart drugs. At the same time the attention was also broadened toward the realization of new organic or inorganic carriers to further improve the cellular internalization of the PNA probes and for co-administration of this oligonucleotide analogues and small drugs to obtain a synergistic effect. The research focus was further extended to the development of modified nucleobase. This was applied to the realization of systems with improved complexation and recognition properties toward the complementary nucleobase. Application of such systems were found in the realization of surface and solution techniques for the detection of single point mutations related to the onset of diseases or for the discrimination of food variety. Later, the focus was also shifted to the realization of devices for fast and sensitive detection of DNA or RNA targets using both surface plasmon resonance based techniques or photonic crystal fibers. In this context the main attention was focused toward the development of systems for PCR-free DNA detection and for miR detection. In the last years, the research interests stretched toward the realization of trigger-responsive systems. The realization of DNA-triggered responsive systems was explored for the creation of new theranostic probes, able to produce at the same a detectable signal for the quantification of a target sequence and impede them to exert their function. Similar system were also applied in the realization of the PNA probes able to release functional molecules when triggered by the recognition of a complementary DNA strand and strengthen the “smart drug” effect by localizing the release of a functional molecule (e.g. fluorophore, cytotoxic molecules, …) only in the target cells. Light-triggerable systems were also explored for the activation of pro-reactive systems able, after activation, to form strong covalent bonds with the target DNA sequence, hence inducing irreversible damage for a stronger inhibitory effect. Finally, after moving to Belgium thanks to a FWO [PEGASUS]² Marie Skłodowska-Curie fellowship, his research was focussed on the exploitation of furan reactivity. The special reactivity of this small aromatic ring was then further explored for the realization of interstrand crosslink systems. The possibility to convert a stable furan to its reactive keto-enal form by localized light-triggered production of singlet oxygen, together with its selective reactivity toward α-effect nucleophiles, is now applied for surface miR-templated detection platforms, and for the realization of a future generation of PNA- or peptide-encoded protein microarrays. In addition, the ability of the furan to partake to the formation of reversible Diels-Alder adduct was recently exploited in the nanomaterial field, for the decoration of gold nanoparticles, for catch-and-release systems. In addition, some of the results obtained in the last three years are exploited for patent filling and for the acquisition of a grant from UGent to further develop the chemistry and for the realization of a prototype. During my career I issued 35 publications, 3 book chapters, and more than 30 other communications to congresses and schools. In 2018, I also received the “Junior Price Organic Chemistry for Life Sciences 2018” from the Organic Chemistry Division of the Italian Chemical Society.

Subject Matter Expertise

• ★ Organic Chemistry
• ★ Peptide Synthesis
• ☆ Supramolecular Chemistry

Services

• Writing
• Research

Writing Technical Writing

Research Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Work Experience

FWO-Marie curie fellow

Ghent University

March 2017 - Present

Post Doc

University of Parma

March 2012 - February 2017

Education

PhD

University of Parma

January 2009 - December 2012

Master degree

University of Parma

October 2006 - July 2008

Certifications

• Certification details not provided.

Publications

OTHER

Alex Manicardi, Enrico Cadoni, Annemieke Madder (2020). Visible-Light Triggered Templated Ligation on Surface Using Furan-Modified PNAs .

Alex Manicardi, Enrico Cadoni, Annemieke Madder (2020). Visible-Light Triggered Templated Ligation on Surface Using Furan-Modified PNAs .

Alex Manicardi, Alessandro Bertucci, Roberto Corradini(2012). Advanced Molecular Probes for Sequence-Specific {DNA} Recognition . 89--124Springer Science $\mathplus$ Business Media

Alex Manicardi, Rosangela Marchelli, Roberto Corradini, Stefano Sforza, Tullia Tedeschi, Enrica Fabbri, Monica Borgatti, Nicoletta Bianchi, Roberto Gambari(2011). Gene Modulation by Peptide Nucleic Acids ({PNAs}) Targeting {microRNAs} ({miRs}) . {InTech}

JOURNAL ARTICLE

(2020). High Levels of Apoptosis Are Induced in the Human Colon Cancer HT-29 Cell Line by Co-Administration of Sulforaphane and a Peptide Nucleic Acid Targeting miR-15b-5p . Nucleic Acid Therapeutics.

Alex Manicardi, Shaiq Sultan, Andrea Rozzi, Jessica Gasparello, Roberto Corradini, Chiara Papi, Alessia Finotti, Ilaria Lampronti, Eva Reali, Giulio Cabrini, et al.(2020). A Peptide Nucleic Acid ({PNA}) Masking the {miR}-145-5p Binding Site of the 3$\prime${UTR} of the Cystic Fibrosis Transmembrane Conductance Regulator ({CFTR}) {mRNA} Enhances {CFTR} Expression in Calu-3 Cells . Molecules. 25. (7). p. 1677. {MDPI} {AG}

Alex Manicardi, Shaiq Sultan, Andrea Rozzi, Jessica Gasparello, Roberto Corradini, Chiara Papi, Alessia Finotti, Ilaria Lampronti, Eva Reali, Giulio Cabrini, et al. (2020). A Peptide Nucleic Acid (PNA) Masking the miR-145-5p Binding Site of the 3′UTR of the Cystic Fibrosis Transmembrane Conductance Regulator (<i>CFTR</i>) mRNA Enhances CFTR Expression in Calu-3 Cells . Molecules.

Alex Manicardi, Enrico Cadoni, Annemieke Madder(2020). {PNA}-Based {MicroRNA} Detection Methodologies . Molecules. 25. (6). p. 1296. {MDPI} {AG}

Alex Manicardi, Enrico Cadoni, Annemieke Madder (2020). PNA-Based MicroRNA Detection Methodologies . Molecules.

(2020). Exploiting Double Exchange Diels-Alder Cycloadditions for Immobilization of Peptide Nucleic Acids on Gold Nanoparticles . Frontiers in Chemistry.

Alex Manicardi, Enrico Cadoni, Annemieke Madder (2020). Visible-light triggered templated ligation on surface using furan-modified PNAs . Chemical Science.

Alex Manicardi, Stefania Croci, Sara Rubagotti, Martina Bonacini, Michele Iori, Pier Cesare Capponi, Gianfranco Cicoria, Maria Parmeggiani, Carlo Salvarani, Annibale Versari, et al.(2019). 64Cu and fluorescein labeled anti-{miRNA} peptide nucleic acids for the detection of {miRNA} expression in living cells . Scientific Reports. 9. (1). Springer Science and Business Media {LLC}

Alex Manicardi, Jessica Gasparello, Alessandro Casnati, Roberto Corradini, Roberto Gambari, Alessia Finotti, Francesco Sansone(2019). Efficient cell penetration and delivery of peptide nucleic acids by an argininocalix[4]arene . Scientific Reports. 9. (1). Springer Science and Business Media {LLC}

Croci, S., Manicardi, A., Rubagotti, S., Bonacini, M., Iori, M., Capponi, P.C., Cicoria, G., Parmeggiani, M., Salvarani, C., Versari, A., et al.(2019). ⁶⁴ Cu and fluorescein labeled anti-miRNA peptide nucleic acids for the detection of miRNA expression in living cells . Scientific Reports. 9. (1).

Mammoli, F., Parenti, S., Lomiento, M., Gemelli, C., Atene, C.G., Grande, A., Corradini, R., Manicardi, A., Fantini, S., Zanocco-Marani, T., et al.(2019). Physiological expression of miR-130a during differentiation of CD34⁺ human hematopoietic stem cells results in the inhibition of monocyte differentiation . Experimental Cell Research. 382. (1).

Milani, R., Brognara, E., Fabbri, E., Manicardi, A., Corradini, R., Finotti, A., Gasparello, J., Borgatti, M., Cosenza, L.C., Lampronti, I., et al.(2019). Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cells . International Journal of Oncology. 55. (1). p. 59-68.

Gasparello, J., Manicardi, A., Casnati, A., Corradini, R., Gambari, R., Finotti, A., Sansone, F.(2019). Efficient cell penetration and delivery of peptide nucleic acids by an argininocalix[4]arene . Scientific Reports. 9. (1).

Fabbri, E., Tamanini, A., Jakova, T., Gasparello, J., Manicardi, A., Corradini, R., Sabbioni, G., Finotti, A., Borgatti, M., Lampronti, I., et al.(2018). A peptide nucleic acid against microrna miR-145-5p enhances the expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells . Molecules. 23. (1).

(2017). A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells . Molecules.

Alex Manicardi, Andrea Rozzi, Saša Korom, Roberto Corradini (2017). Building on the peptide nucleic acid (PNA) scaffold: a biomolecular engineering approach . Supramolecular Chemistry.

(2017). Synthesis and Improved Cross-Linking Properties of C5-Modified Furan Bearing PNAs . Molecules.

Barozzi, M., Manicardi, A., Vannucci, A., Candiani, A., Sozzi, M., Konstantaki, M., Pissadakis, S., Corradini, R., Selleri, S., Cucinotta, A.(2017). Optical Fiber Sensors for Label-Free DNA Detection . Journal of Lightwave Technology. 35. (16). p. 3461-3472.

Mejia-Ariza, R., Rosselli, J., Breukers, C., Manicardi, A., Terstappen, L.W.M.M., Corradini, R., Huskens, J.(2017). DNA Detection by Flow Cytometry using PNA-Modified Metal–Organic Framework Particles . Chemistry - A European Journal. 23. (17). p. 4180-4186.

Elskens, J., Manicardi, A., Costi, V., Madder, A., Corradini, R.(2017). Synthesis and improved cross-linking properties of c5-modified furan bearing pnas . Molecules. 22. (11).

Carvalho, A.M., Manicardi, A., Montes, C.V., Gunnoo, S.B., Schneider, R.J., Madder, A.(2017). Decoration of trastuzumab with short oligonucleotides: Synthesis and detailed characterization . Organic and Biomolecular Chemistry. 15. (42). p. 8923-8928.

Manicardi, A., Rozzi, A., Korom, S., Corradini, R.(2017). Building on the peptide nucleic acid (PNA) scaffold: a biomolecular engineering approach . Supramolecular Chemistry. 29. (11). p. 784-795.

A. M. Carvalho, A. Manicardi, C. V&#233;liz Montes, S. B. Gunnoo, R. J. Schneider, A. Madder(2017). Decoration of trastuzumab with short oligonucleotides: synthesis and detailed characterization . Organic {\&} Biomolecular Chemistry. 15. (42). p. 8923--8928. Royal Society of Chemistry ({RSC})

Brognara, E., Fabbri, E., Montagner, G., Gasparello, J., Manicardi, A., Corradini, R., Bianchi, N., Finotti, A., Breveglieri, G., Borgatti, M., et al.(2016). High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222 . International Journal of Oncology. 48. (3). p. 1029-1038.

Manicardi, A., Bertucci, A., Rozzi, A., Corradini, R.(2016). A Bifunctional Monomer for On-Resin Synthesis of Polyfunctional PNAs and Tailored Induced-Fit Switching Probes . Organic Letters. 18. (21). p. 5452-5455.

Manicardi, A., Gyssels, E., Corradini, R., Madder, A.(2016). Furan-PNA: A mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA . Chemical Communications. 52. (42). p. 6930-6933.

Alex Manicardi, Alessandro Bertucci, Eko Adi Prasetyanto, Dedy Septiadi, Eleonora Brognara, Roberto Gambari, Roberto Corradini, Luisa De Cola(2015). Combined Delivery of Temozolomide and Anti-{miR}221 {PNA} Using Mesoporous Silica Nanoparticles Induces Apoptosis in Resistant Glioma Cells . Small. p. n/a--n/a. Wiley-Blackwell

Alex Manicardi, Alessandro Bertucci, Alessandro Candiani, Sara Giannetti, Annamaria Cucinotta, Giuseppe Spoto, Maria Konstantaki, Stavros Pissadakis, Stefano Selleri, Roberto Corradini(2015). Detection of unamplified genomic {DNA} by a {PNA}-based microstructured optical fiber ({MOF}) Bragg-grating optofluidic system . Biosensors and Bioelectronics. 63. p. 248--254. Elsevier {BV}

Bertucci, A., Prasetyanto, E.A., Septiadi, D., Manicardi, A., Brognara, E., Gambari, R., Corradini, R., De Cola, L.(2015). Combined Delivery of Temozolomide and Anti-miR221 PNA Using Mesoporous Silica Nanoparticles Induces Apoptosis in Resistant Glioma Cells . Small. 11. (42). p. 5687-5695.

Bertucci, A., Manicardi, A., Candiani, A., Giannetti, S., Cucinotta, A., Spoto, G., Konstantaki, M., Pissadakis, S., Selleri, S., Corradini, R.(2015). Detection of unamplified genomic DNA by a PNA-based microstructured optical fiber (MOF) Bragg-grating optofluidic system . Biosensors and Bioelectronics. 63. p. 248-254.

Montagner, G., Gemmo, C., Fabbri, E., Manicardi, A., Accardo, I., Bianchi, N., Finotti, A., Breveglieri, G., Salvatori, F., Borgatti, M., et al.(2015). Peptide nucleic acids targeting β-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells . International Journal of Molecular Medicine. 35. (1). p. 51-58.

Alex Manicardi, Giulia Montagner, Chiara Gemmo, Enrica Fabbri, Igea Accardo, Nicoletta Bianchi, Alessia Finotti, Giulia Breveglieri, Francesca Salvatori, Monica Borgatti, et al.(2014). Peptide nucleic acids targeting $\upbeta$-globin {mRNAs} selectively inhibit hemoglobin production in murine erythroleukemia cells . International Journal of Molecular Medicine. Spandidos Publications

Alex Manicardi, Alessandro Bertucci, Henning L&#252;lf, Dedy Septiadi, Roberto Corradini, Luisa De Cola(2014). Intracellular Delivery of Peptide Nucleic Acid and Organic Molecules Using Zeolite-L Nanocrystals . Advanced Healthcare Materials. 3. (11). p. 1812--1817. Wiley-Blackwell

Alex Manicardi, Eleonora Brognara, Enrica Fabbri, Elena Bazzoli, Giulia Montagner, Claudio Ghimenton, Albino Eccher, Cinzia Cant&#249;, Nicoletta Bianchi, Alessia Finotti, et al.(2014). Uptake by human glioma cell lines and biological effects of a peptide-nucleic acids targeting {miR}-221 . J Neurooncol. 118. (1). p. 19--28. Springer Science $\mathplus$ Business Media

Bertucci, A., L&#252;lf, H., Septiadi, D., Manicardi, A., Corradini, R., De Cola, L.(2014). Intracellular Delivery of Peptide Nucleic Acid and Organic Molecules Using Zeolite-L Nanocrystals . Advanced Healthcare Materials. 3. (11). p. 1812-1817.

Alex Manicardi, Lucia Guidi, Alice Ghidini, Roberto Corradini(2014). Pyrene-modified {PNAs}: Stacking interactions and selective excimer emission in {PNA} 2 {DNA} triplexes . Beilstein Journal of Organic Chemistry. 10. p. 1495--1503. Beilstein Institut

Manicardi, A., Guidi, L., Ghidini, A., Corradini, R.(2014). Pyrene-modified PNAs: Stacking interactions and selective excimer emission in PNA<inf>2</inf>DNA triplexes . Beilstein Journal of Organic Chemistry. 10. p. 1495-1503.

Brognara, E., Fabbri, E., Bazzoli, E., Montagner, G., Ghimenton, C., Eccher, A., Cant&#249;, C., Manicardi, A., Bianchi, N., Finotti, A., et al.(2014). Uptake by human glioma cell lines and biological effects of a peptide-nucleic acids targeting miR-221 . Journal of Neuro-Oncology. 118. (1). p. 19-28.

Manicardi, A., Corradini, R.(2014). Effect of chirality in gamma-PNA: PNA interaction, another piece in the picture . Artificial DNA, PNA &amp; XNA. 5. (3). p. e1131801.

Alex Manicardi, Alessandro Candiani, Alessandro Bertucci, Sara Giannetti, Maria Konstantaki, Stavros Pissadakis, Annamaria Cucinotta, Roberto Corradini, Stefano Selleri(2013). Label-free {DNA} biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating . J. Biomed. Opt. 18. (5). p. 057004. {SPIE}-Intl Soc Optical Eng

Candiani, A., Bertucci, A., Giannetti, S., Konstantaki, M., Manicardi, A., Pissadakis, S., Cucinotta, A., Corradini, R., Selleri, S.(2013). Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating . Journal of Biomedical Optics. 18. (5).

Alex Manicardi, Enrica Fabbri, Tullia Tedeschi, Stefano Sforza, Nicoletta Bianchi, Eleonora Brognara, Roberto Gambari, Rosangela Marchelli, Roberto Corradini(2012). Cellular Uptakes, Biostabilities and Anti-{miR}-210 Activities of Chiral Arginine-{PNAs} in Leukaemic K562 Cells . {ChemBioChem}. 13. (9). p. 1327--1337. Wiley-Blackwell

Alex Manicardi, Alessandro Accetta, Tullia Tedeschi, Stefano Sforza, Rosangela Marchelli, Roberto Corradini(2012). {PNA} bearing 5-azidomethyluracil . Artificial {DNA}: {PNA} {\&} {XNA}. 3. (2). p. 53--62. Informa {UK} Limited

Alex Manicardi, Chiara De Cola, Roberto Corradini, Irene Izzo, Francesco De Riccardis(2012). Carboxyalkyl peptoid {PNAs}: synthesis and hybridization properties . Tetrahedron. 68. (2). p. 499--506. Elsevier {BV}

Brognara, E., Fabbri, E., Aimi, F., Manicardi, A., Bianchi, N., Finotti, A., Breveglieri, G., Borgatti, M., Corradini, R., Marchelli, R., et al.(2012). Peptide nucleic acids targeting miR-221 modulate p27^Kip1 expression in breast cancer MDA-MB-231 cells . International Journal of Oncology. 41. (6). p. 2119-2127.

Manicardi, A., Fabbri, E., Tedeschi, T., Sforza, S., Bianchi, N., Brognara, E., Gambari, R., Marchelli, R., Corradini, R.(2012). Cellular Uptakes, Biostabilities and Anti-miR-210 Activities of Chiral Arginine-PNAs in Leukaemic K562 Cells . ChemBioChem. 13. (9). p. 1327-1337.

De Cola, C., Manicardi, A., Corradini, R., Izzo, I., De Riccardis, F.(2012). Carboxyalkyl peptoid PNAs: Synthesis and hybridization properties . Tetrahedron. 68. (2). p. 499-506.

Manicardi, A., Accetta, A., Tedeschi, T., Sforza, S., Marchelli, R., Corradini, R.(2012). PNA bearing 5-azidomethyluracil: A novel approach for solid and solution phase modification . Artificial DNA: PNA and XNA. 3. (2). p. 1-10.

Alex Manicardi, Roberto Gambari, Enrica Fabbri, Monica Borgatti, Ilaria Lampronti, Alessia Finotti, Eleonora Brognara, Nicoletta Bianchi, Rosangela Marchelli, Roberto Corradini(2011). Targeting {microRNAs} involved in human diseases: A novel approach for modification of gene expression and drug development . Biochemical Pharmacology. 82. (10). p. 1416--1429. Elsevier {BV}

Alex Manicardi, Enrica Fabbri, Tullia Tedeschi, Stefano Sforza, Nicoletta Bianchi, Eleonora Brognara, Alessia Finotti, Giulia Breveglieri, Monica Borgatti, Roberto Corradini, et al.(2011). Modulation of the Biological Activity of {microRNA}-210 with Peptide Nucleic Acids ({PNAs}) . {ChemMedChem}. 6. (12). p. 2192--2202. Wiley-Blackwell

Alex Manicardi, Roberto Corradini, Stefano Sforza, Tullia Tedeschi, Filbert Totsingan, Rosangela Marchelli(2011). Peptide Nucleic Acids with a Structurally Biased Backbone. Updated Review and Emerging Challenges . {CTMC}. 11. (12). p. 1535--1554. Bentham Science Publishers Ltd.

Alex Manicardi, Tullia Tedeschi, Alessandro Calabretta, Mariangela Bencivenni, Giandomenico Corrado, Martina Caramante, Roberto Corradini, Rosa Rao, Stefano Sforza, Rosangela Marchelli(2011). A {PNA} microarray for tomato genotyping . Mol. {BioSyst}. 7. (6). p. 1902. Royal Society of Chemistry ({RSC})

Fabbri, E., Manicardi, A., Tedeschi, T., Sforza, S., Bianchi, N., Brognara, E., Finotti, A., Breveglieri, G., Borgatti, M., Corradini, R., et al.(2011). Modulation of the Biological Activity of microRNA-210 with Peptide Nucleic Acids (PNAs) . ChemMedChem. 6. (12). p. 2192-2202.

Alex Manicardi, Eleonora Brognara, Ilaria Lampronti, Giulia Breveglieri, Alessandro Accetta, Roberto Corradini, Monica Borgatti, Alessandro Canella, Chiara Multineddu, Rosangela Marchelli, et al.(2011). C(5) modified uracil derivatives showing antiproliferative and erythroid differentiation inducing activities on human chronic myelogenous leukemia K562 cells . European Journal of Pharmacology. 672. (1-3). p. 30--37. Elsevier {BV}

Gorska, K., Manicardi, A., Barluenga, S., Winssinger, N.(2011). DNA-templated release of functional molecules with an azide-reduction- triggered immolative linker . Chemical Communications. 47. (15). p. 4364-4366.

Tedeschi, T., Calabretta, A., Bencivenni, M., Manicardi, A., Corrado, G., Caramante, M., Corradini, R., Rao, R., Sforza, S., Marchelli, R.(2011). A PNA microarray for tomato genotyping . Molecular BioSystems. 7. (6). p. 1902-1907.

Corradini, R., Sforza, S., Tedeschi, T., Totsingan, F., Manicardi, A., Marchelli, R.(2011). Peptide nucleic acids with a structurally biased backbone. Updated review and emerging challenges . Current Topics in Medicinal Chemistry. 11. (12). p. 1535-1554.

Fabbri, E., Brognara, E., Borgatti, M., Lampronti, I., Finotti, A., Bianchi, N., Sforza, S., Tedeschi, T., Manicardi, A., Marchelli, R., et al.(2011). miRNA therapeutics: Delivery and biological activity of peptide nucleic acids targeting miRNAs . Epigenomics. 3. (6). p. 733-745.

Brognara, E., Lampronti, I., Breveglieri, G., Accetta, A., Corradini, R., Manicardi, A., Borgatti, M., Canella, A., Multineddu, C., Marchelli, R., et al.(2011). C(5) modified uracil derivatives showing antiproliferative and erythroid differentiation inducing activities on human chronic myelogenous leukemia K562 cells . European Journal of Pharmacology. 672. (1-3). p. 30-37.

Gambari, R., Fabbri, E., Borgatti, M., Lampronti, I., Finotti, A., Brognara, E., Bianchi, N., Manicardi, A., Marchelli, R., Corradini, R.(2011). Targeting microRNAs involved in human diseases: A novel approach for modification of gene expression and drug development . Biochemical Pharmacology. 82. (10). p. 1416-1429.

Alex Manicardi, Enrica Fabbri, Eleonora Brognara, Monica Borgatti, Ilaria Lampronti, Alessia Finotti, Nicoletta Bianchi, Stefano Sforza, Tullia Tedeschi, Rosangela Marchelli, et al.(2011). {miRNA} therapeutics: delivery and biological activity of peptide nucleic acids targeting {miRNAs} . Epigenomics. 3. (6). p. 733--745. Future Medicine Ltd

Alex Manicardi, Katarzyna Gorska, Sofia Barluenga, Nicolas Winssinger(2011). {DNA}-templated release of functional molecules with an azide-reduction-triggered immolative linker . Chemical Communications. 47. (15). p. 4364. Royal Society of Chemistry ({RSC})

Alex Manicardi, Alessandro Calabretta, Mariangela Bencivenni, Tullia Tedeschi, Stefano Sforza, Roberto Corradini, Rosangela Marchelli(2010). Affinity and selectivity of C2- and C5-substituted {\textquotedblleft}chiral-box{\textquotedblright} {PNA} in solution and on microarrays . Chirality. 22. (1E). p. E161--E172. Wiley-Blackwell

Manicardi, A., Calabretta, A., Bencivenni, M., Tedeschi, T., Sforza, S., Corradini, R., Marchelli, R.(2010). Affinity and selectivity of C2- and C5-substituted "chiral-box" PNA in solution and on microarrays . Chirality. 22. (1 E).

BOOK CHAPTER

Manicardi, A., Gambari, R., de Cola, L., Corradini, R.(2018). Preparation of anti-miR PNAs for drug development and nanomedicine . Methods in Molecular Biology. 1811. p. 49-63.

CONFERENCE PAPER

Candiani, A., Salloom, H.T., Coscelli, E., Sozzi, M., Manicardi, A., Ahmad, A.K., Al-Janabi, A.H., Corradini, R., Picchi, G., Cucinotta, A., et al.(2014). Bio-functionalized hollow core photonic crystal fibers for label-free DNA detection . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 8938.

Candiani, A., Giannetti, S., Cucinotta, A., Bertucci, A., Manicardi, A., Konstantaki, M., Margulis, W., Pissadakis, S., Corradini, R., Selleri, S.(2013). DNA biosensors implemented on PNA-functionalized microstructured optical fibers Bragg gratings . Proceedings of SPIE - The International Society for Optical Engineering. 8775.