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Hire Dr. Prabir K.

Portugal

USD 60 /hr

Research Scientist with 10+ years of experience in Life science, Biotechnology and Interdisciplinary science

Profile Summary

Experienced and motivated bioengineer/biotechnologist with strong background in nanobiotechnology, biosensor and point of care (POC) diagnostics. Interested in life sciences research opportunities with broad application in technology development and translational research. Key Strengths • Adaptability in diverse scientific areas and aptitude for interdisciplinary skills • Experienced in oral and written scientific communication, meticulous documentation and reporting • Ability to conceptualize, design, optimize and troubleshoot projects

Subject Matter Expertise

• ★ Nanobiotechnology
• ★ In vitro Diagnostics
• ★ Lab-on-a-Chip Devices
• ☆ Surface Science
• ☆ Nanotechnology & Nanomaterials
• ☆ Agricultural Chemistry
• ☆ Nanotechnology
• ☆ Virology
• ☆ Animal Biotechnology
• ☆ Molecular Biology
• ☆ Microarrays
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Next Generation Sequencing (NGS)
• ☆ ATAC-Seq
• ☆ Protein Sequencing
• ☆ Recombinant DNA
• ☆ RNA Sequencing
• ☆ DNA/Gene Synthesis
• ☆ Monoclonal Antibody Production
• ☆ Cell Biology
• ☆ Cytochemistry
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Biomedical Engineering
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Electron Microscopy
• ☆ Fluorescence Microscopy
• ☆ Food Microbiology
• ☆ Food Chemistry
• ☆ Microfluidics
• ☆ Biosensors
• ☆ Agricultural Biotechnology
• ☆ Blotting
• ☆ Vaccine Development
• ☆ Medical Devices
• ☆ Medical Device Development
• ☆ Biocompatibility Testing
• ☆ Medical Device Design
• ☆ Medical Device Prototyping
• ☆ Medical Device Regulatory Consulting
• ☆ EU In vitro Diagnostic Regulation (IVDR) Compliance
• ☆ In vitro Diagnostic Validation
• ☆ In vitro Diagnostic Design
• ☆ Point-of-Care Devices
• ☆ Biomarker Analysis

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing, Copywriting, Newswriting

Research Market Research, Fact Checking, Gap Analysis, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis

Product Development Formulation, Product Validation, Concept Development, Device Fabrication

Work Experience

Marie Sklodowska-Curie Fellow

International Iberian Nanotechnology Laboratory

February 2023 - Present

Postdoctoral Research Fellow

University of Oxford

December 2021 - November 2022

Queen's University Belfast

- November 2021

Postdoctoral Research Fellow

Queen's University Belfast

October 2020 - November 2021

Post Doctoral Fellow

University of Ottawa

January 2018 - June 2019

Pondicherry University

August 2009 - September 2010

Education

Ph.D (BIOTECHNOLOGY)

Agharkar Research Institute

October 2010 - July 2017

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Clare Mills, Michael J. Dillon, Prabir Kumar Kulabhusan, Diana Senovilla-Herrero, Katrina Campbell (2022). Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins . Environmental Science & Technology.

Prabir Kumar Kulabhusan(2022). Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins . Environmental Science &Technology. American Chemical Society ({ACS})

(2022). Aptamer-based Emerging Tools for Viral Biomarker Detection: A focus on SARS-CoV-2 . Current Medicinal Chemistry.

Prabir Kumar Kulabhusan and Anugrah Tripathi and Krishna Kant(2022). Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry . Sensors. 22. (3). p. 1259. {MDPI} {AG}

Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry @article{6de93f7d09044ef886ef28e5698e4a97, title = "Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry", abstract = "Plant diseases and their diagnoses are currently one of the global challenges and causes significant impact to the economy of farmers and industries depending on plant-based products. Plant pathogens such as viruses, bacteria, fungi, and pollution caused by the nanomaterial, as well other important elements of pollution, are the main reason for the loss of plants in agriculture and in forest ecosystems. Presently, various techniques are used to detect pathogens in trees, which includes DNA-based techniques, as well as other microscopy based identification and detection. However, these methodologies require complex instruments and time. Lately, nanomaterial-based new biosensing systems for early detection of diseases, with specificity and sensitivity, are developed and applied. This review highlights the nanomaterial-based biosensing methods of disease detection. Precise and time effective identification of plant pathogens will help to reduce losses in agriculture and forestry. This review focuses on various plant diseases and the requirements for a reliable, fast, and cost-effective testing method, as well as new biosensing technologies for the detection of diseases of field plants in forests at early stages of their growth.", keywords = "Biosensing Techniques, Metal Nanoparticles, Gold, Prospective Studies, plant pathogen biosensing, Ecosystem, forest diseases, plant disease, Forestry, gold nanoparticles, Plant Diseases", author = "Kulabhusan, {Prabir Kumar} and Anugrah Tripathi and Krishna Kant", year = "2022", month = feb, day = "7", doi = "10.3390/s22031259", language = "English", volume = "22", journal = "Sensors (Basel, Switzerland)", issn = "1424-8220", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", number = "3", } . Sensors (Basel, Switzerland).

Kulabhusan, Prabir Kumar, Ray, Rohitraj, Ramachandra, Suma G., Srinivasulu, Maddasani, Hariharan, Arumugam, Balaji, Krishnasamy, Mani, Naresh Kumar (2022). Coalescing aptamers and liquid-crystals for sensing applications . Microchemical Journal.

Recent trends in the detection of freshwater cyanotoxins with a critical note on their occurrence in Asia @article{60b7802177124a18b8441736f6a8428f, title = "Recent trends in the detection of freshwater cyanotoxins with a critical note on their occurrence in Asia", abstract = "Cyanobacteria are highly prevalent in slow-moving and nutrient-rich water bodies due to changing climatic conditions, eutrophication, and anthropogenic activities. Toxins of cyanobacterial blooms, i.e., cyanotoxins are also increasing at alarming rates in freshwater. Several efforts are being taken to detect cyanotoxins using molecular and analytical techniques to understand their occurrence and distribution, however, these studies are discrete and localized. The detection of cyanotoxins within water bodies is a long-established challenge. In this article, conventional methods of detection and the recently used nanostructure based immuno-sensors are described. Several studies are considered where aptamers are utilised as the biorecognition probe and we have discussed their use in colorimetric, electrochemical and optical sensors for the detection of cyanotoxins. Furthermore, this article also reviews the current field deployable diagnostics for the real-time monitoring of cyanotoxins. Future work on fundamental studies, development of highly specific aptamers for recognising different congeners of cyanotoxins and the integration of sensors into portable or lab-on-a-chip devices could be interesting and useful research. Moreover, several studies related to the occurrence and distribution of cyanotoxins in the freshwater bodies of Asia are reviewed, with potential threats identified. This article also adds a note on the geographical distribution and detection of cyanotoxins in Asia. Thus, we have provided an overview of various evolving detection systems that could be employed in identified problematic underdeveloped regions to improve management strategies to monitor and control cyanotoxins.", keywords = "Cyanotoxins, Freshwater, Detection methods, Biosensors, Aptamers, Asia", author = "Kulabhusan, {Prabir Kumar} and Katrina Campbell", year = "2021", month = dec, day = "1", doi = "10.1016/j.teac.2021.e00150", language = "English", volume = "32", journal = "Trends in Environmental Analytical Chemistry", issn = "2214-1588", publisher = "Elsevier BV", } . Trends in Environmental Analytical Chemistry.

Prabir Kumar Kulabhusan, Katrina Campbell (2021). Recent trends in the detection of freshwater cyanotoxins with a critical note on their occurrence in Asia . Trends in Environmental Analytical Chemistry.

Recent trends in the detection of freshwater cyanotoxins with a critical note on its occurrence in Asia @article{2021, doi = {10.1016/j.teac.2021.e00150}, url = {https://doi.org/10.1016%2Fj.teac.2021.e00150}, year = 2021, month = {oct}, publisher = {Elsevier {BV}}, pages = {e00150}, author = {Prabir Kumar Kulabhusan and Katrina Campbell}, title = {Recent trends in the detection of freshwater cyanotoxins with a critical note on its occurrence in Asia}} . Trends in Environmental Analytical Chemistry.

Yuchu Dou and Lixuan Ren and Prabir Kumar Kulabhusan and Emil A. Zaripov and Maxim V. Berezovski(2021). Quantitative Capillary Electrophoresis for Analysis of Extracellular Vesicles (EVqCE) . Separations. 8. (8). p. 110. {MDPI} {AG}

Prabir Kulabhusan, Yuchu Dou, Lixuan Ren, Emil Zaripov, Maxim Berezovski (2021). Quantitative Capillary Electrophoresis for Analysis of Extracellular Vesicles (EVqCE) . Separations.

Yuchu Dou, Lixuan Ren, Prabir Kumar Kulabhusan, Emil A. Zaripov, Maxim Berezovski (2021). Quantitative Capillary Electrophoresis for Analysis of Extracellular Vesicles (EVqCE) . Separations.

(2020). In silico studies on the interaction of phage displayed biorecognition element (TFQAFDLSPFPS) with the structural protein VP28 of white spot syndrome virus . Journal of Molecular Modeling.

Prabir Kumar Kulabhusan, Babar Hussain, Meral Yüce (2020). Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents . Pharmaceutics.

Prabir Kumar Kulabhusan, Babar Hussain, MERAL YUCE (2020). Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents . Pharmaceutics.

(2020). Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents . Pharmaceutics.

Kumar Kulabhusan, Prabir, Hussain, Babar, Yuce, Meral (2020). Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents . Pharmaceutics.

Jeevanandam, Jaison, Solanki, Ashish Kumar, Sharma, Shailza, Kulabhusan, Prabir Kumar, Pahil, Sapna, Danquah, Michael K. (2019). Synthesis of Bioactive Peptides for Pharmaceutical Applications . Pharmaceutical Biocatalysis: Fundamentals, Enzyme Inhibitors, and Enzymes in Health and Diseases.

Field-Usable Lateral Flow Immunoassay for the Rapid Detection of White Spot Syndrome Virus (WSSV) @article{10.1371/journal.pone.0169012, author= {Kulabhusan, Prabir Kumar AND Rajwade, Jyutika M. AND Sugumar, Vimal AND Taju, Gani AND Sahul Hameed, A. S. AND Paknikar, Kishore M.}, journal= {PLOS ONE}, publisher= {Public Library of Science}, title= {Field-Usable Lateral Flow Immunoassay for the Rapid Detection of White Spot Syndrome Virus (WSSV)}, year= {2017}, month= {01}, volume= {12}, url= {https://doi.org/10.1371/journal.pone.0169012}, pages= {1-14}, abstract= {Background White spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagnostic method for the detection of WSSV infection is imperative to prevent huge economic losses. Methods/Principal Findings Here, we report on the development of a lateral flow immunoassay (LFIA) employing gold nanoparticles conjugated to a polyclonal antibody against VP28 (envelope protein of WSSV). The LFIA detected WSSV in ~20 min and showed no cross-reactivity with other shrimp viruses, viz. Monodon Baculovirus (MBV), Hepatopancreatic parvovirus (HPV) and Infectious Hypodermal and Hematopoietic Necrosis virus (IHHNV). The limit of detection (LOD) of the assay, as determined by real-time PCR, was 103 copies of WSSV. In a time course infectivity experiment, ~104 WSSV particles were injected in Litopenaeus vannamei. The LFIA could rapidly (~ 20 min) detect the virus in different tissues after 3 h (hemolymph), 6 h (gill tissue) and 12 h (head soft tissue, eye stalk, and pleopod) of infection. Based on these findings, a validation study was performed using 75 field samples collected from different geographical locations in India. The LFIA results obtained were compared with the conventional “gold standard test”, viz. one-step PCR. The analysis of results in 2x2 matrix indicated very high sensitivity (100%) and specificity (96.77%) of LFIA. Similarly, Cohen’s kappa coefficient of 0.983 suggested "very good agreement” between the developed LFIA and the conventional one-step PCR. Conclusion The LFIA developed for the rapid detection of WSSV has an excellent potential for use in the field and could prove to be a boon to the aquaculture industry.}, number= {1}, doi= {10.1371/journal.pone.0169012}} . PLOS ONE.

Kulabhusan, Prabir Kumar, Rajwade, Jyutika M., Hameed, A. S. Sahul, Paknikar, Kishore M. (2017). Lateral flow assay for rapid detection of white spot syndrome virus (WSSV) using a phage-displayed peptide as bio-recognition probe . Applied Microbiology and Biotechnology.

Kulabhusan, Prabir Kumar, Rajwade, Jyutika M., Sugumar, Vimal, Taju, Gani, Hameed, A. S. Sahul, Paknikar, Kishore M. (2017). Field-Usable Lateral Flow Immunoassay for the Rapid Detection of White Spot Syndrome Virus (WSSV) . Plos One.

Kulabhusan, Prabir Kumar and Rajwade, Jyutika M. and Sahul Hameed, A. S. and Paknikar, Kishore M.(2017). Lateral flow assay for rapid detection of white spot syndrome virus (WSSV) using a phage-displayed peptide as bio-recognition probe . Applied Microbiology and Biotechnology. p. 1--11.

Shailaja Agrawal and Prabir Kumar Kulabhusan and Manali Joshi and Dhananjay Bodas and Kishore M. Paknikar(2016). A high affinity phage-displayed peptide as a recognition probe for the detection of Salmonella Typhimurium . Journal of Biotechnology. 231. p. 40 - 45.

Agrawal, Shailaja, Kulabhusan, Prabir Kumar, Joshi, Manali, Bodas, Dhananjay, Paknikar, Kishore M. (2016). A high affinity phage-displayed peptide as a recognition probe for the detection of Salmonella Typhimurium . Journal of Biotechnology.

Nanoformulated Herbal Drug Delivery as Efficient Antidotes Against Systemic Poisons .

BOOK CHAPTER

Milad Torabfam and Qandeel Saleem and Prabir Kumar Kulabhusan and Mustafa Kemal Bayaz{\i}t and Meral Yüce(2022). Application of Nanomaterials in Food Quality Assessment . Nanosensing and Bioanalytical Technologies in Food Quality Control. p. 1--44. Springer Singapore

Prajakta Tambe and Shamala Mane and Bhushan P. Chaudhari and Prabir Kumar Kulabhusan(2022). Carbon nanomaterials for therapeutic applications . Fundamentals of Bionanomaterials. p. 293--325. Elsevier

(2021). Chapter 6: Characterization of Biological Molecule–Loaded Nanostructures Using Circular Dichroism and Fourier Transform Infrared Spectroscopy. Drug Delivery with Targeted Nanoparticles: In Vitro and In Vivo Evaluation Methods 1st Edition.

Godfred Sabbih and Prabir Kumar Kulabhusan and Rohit Kumar Singh and Jaison Jeevanandam and Michael K. Danquah(2021). Biocomposites for the fabrication of artificial organs . Green Biocomposites for Biomedical Engineering. p. 301--328. Elsevier

Prabir Kumar Kulabhusan and Shailaja Agrawal and Jaison Jeevanandam and Michael K. Danquah(2020). Nanoformulated Herbal Drug Delivery as Efficient Antidotes Against Systemic Poisons . Poisonous Plants and Phytochemicals in Drug Discovery. p. 269--294. Wiley

(2019). Synthesis of Bioactive Peptides for Pharmaceutical Applications. Pharmaceutical Biocatalysis - Fundamentals, Enzyme Inhibitors, and Enzymes in Health and diseasesEdition: 1Chapter: 6 Publisher: Pan Stanford Publishing.

(2019). Biofunctional Nanoparticles for Protein Separation, Purification and Detection . Horizons in Bioprocess Engineering.