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Hire Dr. Poulami S.

Profile Summary

Research Experience Research Associate, The Rockefeller University Adviser – Luciano Marraffini, Ph.D. Project – Biochemical characterization of CRISPR machinery 2012 - Present Developed in vitro assays to characterize the type IIIA multiprotein complex involved in DNA targeting in CRISPR interference Discovered a novel protein complex involved in CRISPR adaptation Used Cas9 to achieve programmable transcription activation/repression Identified the biochemical functions of key Cas proteins As the sole protein biochemist in my current lab, I established our current pipeline for protein expression, purification, and assay. This pipeline involves recombinant expression, FPLC purification, and enzymological assays involving 32P and fluorescence monitoring Supervised graduate students as well as post docs in these fundamentals Presented results in Infectious disease retreat in Rockefeller University, Gordon conference as well as the CRISPR conference 2014, 2015 Graduate Student, Sloan-Kettering Institute 2008-2012 Adviser – Stewart Shuman, M.D, Ph.D. Thesis: Lessons in ligation, structure function studies of a minimal pluripotent DNA ligase. Conducted structure function analysis of minimized eukaryal ATP-dependent DNA ligase using biochemistry and yeast genetic techniques Used stopped-flow and fast quench instrumentation to probe millisecond kinetic processes !Extensively used MATLAB to perform kinetic data analysis Successfully crystallized and solved crystal structures of different proteins.

Subject Matter Expertise

• ☆ Chemistry
• ☆ Separation & Purification
• ☆ Crystallography
• ☆ Microbiology
• ☆ Biochemistry
• ☆ Biochemical Assays
• ☆ Structural Biology
• ☆ Molecular Biology
• ☆ Genetics
• ☆ Biotechnology & Bioengineering
• ☆ Microbial Fermentation
• ☆ Gene Editing/CRISPR
• ☆ Protein/Enzyme Engineering
• ☆ Matlab
• ☆ Infectious Disease
• ☆ Audio Transcription

Services

• Consulting
• Product Development

Work Experience

Research Associate

Rockefeller University

July 2012 - Present

Graduate Student

Sloan-Kettering Institute

June 2008 - June 2012

Education

Ph.D (Cornell-Rockefeller-Sloan-Kettering Tri-Institutional Program in Chemical Biology (TPCB))

Joan and Sanford I Weill Medical College of Cornell University

June 2007 - June 2012

Ph.D (Cornell-Rockefeller-Sloan-Kettering Tri-Institutional Program in Chemical Biology (TPCB))

Joan and Sanford I Weill Medical College of Cornell University

June 2007 - June 2012

Master of Science (M.S.) in Chemistry (Chemistry)

Indian Institute of Technology Kanpur

June 2005 - May 2007

Bachelor of Science (B.S.) with Honors (Chemistry)

Presidency University

June 2002 - May 2005

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity. Cell.

(2015). Cas9 specifies functional viral targets during the adaptive phase of CRISPR-Cas immunity . Nature.

(2014). Genetic characterization of antiplasmid immunity through a type III-A CRISPR-Cas system . J of Bacteriology.

(2013). A ruler protein in a complex for antiviral defense determines the length of small interfering CRISPR RNAs . J Biol Chem.

(2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system . Nucleic Acids Res.

(2012). Kinetic analysis of DNA strand joining by Chlorella virus DNA ligase and the role of nucleotidyltransferase motif VI in ligase adenylylation . J Biol Chem.

(2011). Structure-function analysis of the OB and latch domains of chlorella virus DNA ligase . J Biol Chem.

(2011). Functional dissection of the DNA interface of the nucleotidyltransferase domain of chlorella virus DNA ligase . J Biol Chem.

(2010). Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris . Acta Crystallogr Sect F Struct Biol Cryst Commun.