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Contact Michael D.

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Profile Details

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Hire Dr. Michael D.

United States

USD 50 /hr

Expert Viral Vector Production and Purification: end to end bioprocess and trouble shooting

Profile Summary

Michael Dibiasio-White is an expert in the viral vector and infectious vector field. He has his Ph.D from Purdue University in Molecular Virology and his masters from Quinnipiac/Yale University in Molecular and Cellular Biology. With over 15 years of virology and molcular experience along with over 5 years of process experience for biologicis including AAV. He has been invited to both round tables and given talks at conferences focused on next generation technologies and analytic capabilities for viral vectors as well as advise on the current field of upsteam and downstream process science and where there are gaps that need to be addressed. During his time, he has worked on end to end vector design, production and purification for AAV and other ssDNA viral vectors specializing in desiging and implementing high quality processes that yield not only high producing vector but highly potentent vector. He has established workflows utilizing depth filtration, TFF, IEX (E/F seperation) HPLC based assays for biophysical evalution and characterization of AAV products as well. His latest work at Affinia is focused on building the process development team and establishing viral vector screening workflows and candidate selection crteria for manufacturability analysis while also coordianting with tech ops to evalute CDMOs and establish SOWs for intenral and external PD studies and tox lot and engineering lot productions.

Molecular virologist by training with extensive experience in analytical, molecular, and biochemical analysis of virus expression systems. I am skilled in understanding virus biology and applying this knowledge towards the design of optimal viral vector designs. In addition, I have taken this knowledge and applied it towards developing expertise in upstream and downstream viral vector process development including analytical/biophysical characterizations of purified vectors.

• 8+ years' experience in combined industry and academic settings in infectious virus (RNA virus and viral replicon development), viral vectors (AAV/others) and viral protein expression/purification as well as plasmid design and optimization for increased vector and viral protein yields (AKTA-FPLC chromatography systems) in BLS2+ environments.

• Expertise in manipulation of viral genetic systems and development of molecular and biochemical assays to investigate various segments of the viral lifecycle.

• Skilled in upstream production/expression,
purification and characterization of both enveloped and non-enveloped virus/vectors.

•Team leader with demonstrated success in leading highly visible cross functional teams and projects in the gene therapy arena.

• Demonstrated ability to discuss/present complex scientific ideas as evidenced by multiple oral presentations and coordinated multi-lab collaborations dealing with several projects simultaneously.

Specialties: AAV biology and skilled in end to end bioprocess development for viral vectors, Flavivirus biology and virus expression systems, purification and process development for preclinical material using several modalities (ultracentrifugation, membrane/affinity chromatography, IEX, TFF/UFDF).

Subject Matter Expertise

Services

Writing
Research
Consulting
Product Development

Writing Technical Writing

Research Market Research, Feasibility Study, Fact Checking, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Product Development Formulation

Work Experience

Sr. Scientist Bioprocess: Cellular and Vector Engineering

Ring Therapeutics: Viral Vector Gene Therapy Company

June 2019 - Present

Sr. Fellow: Bioprocess/Vector and protein engineering

AstraZeneca/Medimmune

June 2018 - June 2019

Research Fellow: Structure and Molecular Virology

Purdue University

August 2013 - June 2018

Postgraduate Fellow: Hepatitis C Virus

Yale University Medical School

June 2012 - June 2013

Education

Ph.D Structural and Molecular Virology

Purdue University

August 2013 - July 2018

Certifications

Certification details not provided.