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Contact Fabio D.

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Profile Details

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USD 12 /hr

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Hire Dr. Fabio D.

Italy

USD 12 /hr

Ph.D. in systems medicine-human genetics | 5+ y retinal gene therapy | Love to talk about science!

Profile Summary

- Second author and eighth author in two published articles (see profile)
- 5+ years of experience in retinal gene therapy using adeno-associated viral vectors (AAV) and dual AAV
- 4+ years of experience about in vivo testing of retinal functionality (electroretinogram), morphology (optical coherence tomography) and funduscopy on live rodents and porcine
- 3+ years of experience in retinal histology by criosections, paraffine sections and resina sections from 10-um thick sections to 0.5 um thin sections
- 1+ year of experience with CRISPR/Cas9 and modified nucleases for genome editing in vitro and in vivo in retina and liver
- Performed automated high-throughput screening of small compounds libraries in vitro using Opera automated system
- Designed and carried western blot analysis in vitro and in vivo on mouse tissues
- Perfomed cloning of plasmidic and PCR fragments DNA
- Cultivated immortalized cell lines for DNA transfection or AAV infection
- Perfomed in vitro siRNA-mediated knock-down
- Performed PCR and quantitative real-time PCR
- Prepared SOPs and reports of pre-clinical studies 
- Prepared scientific articles and presentations using Adobe Illustrator and Powerpoint.
- Mice handling, mice sex determination and intraperitoneal injections from post-natal day 1
- Data analysis in Excel and GraphPad

Subject Matter Expertise

Services

Writing
Research
Consulting
Data & AI

Writing Technical Writing

Research Feasibility Study

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization

Work Experience

Postdoctorante

Telethon Institute of Genetics and Medicine (TIGEM)

October 2021 - Present

Ph.D. student in Systems Medicine (Thesis Gene therapy for Girate atrophy of the choroid and retina and for Usher Syndrome type 1B retinitis pigmentosa)

Tigem (Telethon Institute of Genetics and Medicine)

October 2017 - September 2021

Traning in rodents and porcine retinal electrophysiology

University of l'Aquila-Tigem (Telethon Institute of Genetics and Medicine)

April 2017 - September 2017

Master thesis on high throughput screening of small molecules that enhance AAV gene therapy

Tigem (Telethon Institute of Genetics and Medicine)

March 2015 - March 2017

Education

Master of Science in Medical Biotechnologies

University of Naples Federico II

January 2014 - January 2017

Bachelor of Science in Human Biotechonologies

University of Naples Federico II

January 2010 - January 2014

Certifications

Certification details not provided.

Publications

JOURNAL ARTICLE

(2019). Intein-mediated protein trans-splicing expands adeno-associated virus transfer capacity in the retina . Science Translational Medicine. 11. (492). p. eaav4523. American Association for the Advancement of Science ({AAAS})

(2018). High-Throughput Screening Identifies Kinase Inhibitors That Increase Dual Adeno-Associated Viral Vector {TransductionIn} Vitroand in Mouse Retina . Human Gene Therapy. 29. (8). p. 886--901. Mary Ann Liebert Inc