Profile Details

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Hire Dr. EDEN REBECCA P.

South Africa

USD 100 /hr

Enzymologist

Profile Summary

Dr. Padayachee’s passion is ENZYMES! She absolutely loves figuring out enzyme mechanisms, catalysis, kinetics and their function. As a structural biochemist and enzymologist, she enjoys studying enzyme-ligand binding, by using a suite of computational docking software e.g. AutoDock. Biological thermodynamics, spectroscopy, spectrofluorimetry, surface plasmon resonance, electron microscopy are some of the techniques she has used in studying ligand binding phenomena. She strongly believes that enzyme-ligand binding is the basis for future drug discovery as how proteins bind and their subsequent conformational changes are the keys to unlocking the design of therapeutic agents to target protein misfolding diseases. Amyloid fibrils and neurofibrillary tangles are hall marks of Alzheimer’s disease. In her Ph.D thesis she developed new therapeutic strategies in the diagnosis of Alzheimer’s disease by investigating the potential of the enzyme nitric oxide synthase as a catalyst for amyloid fibril formation. She discovered that nanoparticles reverse amyloid fibril formation. Her current position as a post doctorate focused on identifying high density lipoprotein (HDL) particles in cerebrospinal fluid and its effect on reducing amyloid aggregation in AD. Amyloid beta and Tau has been found to interact and form a complex at the synapse. A small molecule inhibitor that prevents the formation of this complex is ideal. She would like to apply the use of endogenous HDL as a “disaggregating agent" to block this complex from forming in the first instance. Targeting the clearance of Aβ and Tau simultaneously affords a combination therapy approach that could be very promising in the field of AD. She believes that imagination is more creative than knowledge and enjoys stimulating innovative thought in young minds by encouraging scientists to step out of their comfort zone and seek challenges at the interface between academia and industry

Subject Matter Expertise

• ★ Liquid Chromatography/HPLC
• ★ Protein/Enzyme Engineering
• ☆ STEM
• ☆ Molecular Biology
• ☆ Proteomics
• ☆ Mutagenesis
• ☆ Neurochemistry
• ☆ Biophysics
• ☆ Recombinant Protein Expression
• ☆ Book Reviews
• ☆ Humanities Education
• ☆ Skin Cancer
• ☆ Cancer Research

Services

• Writing
• Research
• Consulting
• Product Development

Work Experience

Brain Based Coach

Neuroleadership Institute

July 2020 - Present

Associate Medical Writer

ApotheCom: A Huntsworth Health Company

April 2021 - September 2021

Postdoctoral Scholar

University of Kentucky

January 2019 - June 2020

Postdoctoral Fellow

Institute of Diagnostic Medicine & Infectious Diseases, Capetown South Africa

June 2016 - December 2018

Postdoctoral Fellow

University of Kwa-Zulu Natal, South Africa

January 2016 - June 2016

Postdoctoral Fellow

Institute of Neurochemistry-Sweden

January 2014 - November 2015

Education

PhD Medicinal Biochemistry

Rhodes University

January 2010 - December 2012

Masters in Science: Medicinal Biochemistry

Rhodes University

January 2008 - December 2009

Honours in Chemistry

Rhodes University

January 2007 - December 2007

Bachelor of Science Chemistry & Biochemistry

Rhodes University

January 2004 - December 2006

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2019). Applications of SNAP-tag technology in skin cancer therapy . Health Science Reports.

(2019). Kinetic and thermodynamic characterisation of HIV-protease inhibitors against E35D↑G↑S mutant in the South African HIV-1 subtype C protease . Journal of Enzyme Inhibition and Medicinal Chemistry.

(2019). Kinetic and thermodynamic characterisation of HIV-protease inhibitors against E35D↑G↑S mutant in the South African HIV-1 subtype C protease . Journal of Enzyme Inhibition and Medicinal Chemistry.

(2017). Human Antibody Fusion Proteins/Antibody Drug Conjugates in Breast and Ovarian Cancer . Transfusion Medicine and Hemotherapy.

(2016). Cerebrospinal fluid-induced retardation of amyloid β aggregation correlates with Alzheimer's disease and the APOE ε4 allele . Brain Research.

(2013). Etiology of Alzheimer’s disease: Kinetic, thermodynamic and fluorimetric analyses of interactions of pseudo Aβ-peptides with neuronal nitric oxide synthase . Neuropeptides.

(2013). Interaction of glycine zipper fragments of Aβ-peptides with neuronal nitric oxide synthase: Kinetic, thermodynamic and spectrofluorimetric analysis . Neuropeptides.

(2012). Association of β-amyloid peptide fragments with neuronal nitric oxide synthase: Implications in the etiology of Alzheimers disease . Journal of Enzyme Inhibition and Medicinal Chemistry.

(2011). Spectrofluorimetric analysis of the interaction of amyloid peptides with neuronal nitric oxide synthase: Implications in Alzheimer's disease . Biochimica et Biophysica Acta (BBA) - General Subjects.