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Hire Deependra Kumar S.

India

Data analysis, Manuscript and report writing, editing, publication and presentation

Profile Summary

Currently, I lead a team focused on ex vivo assays and biomarker discovery and development, managing projects, client communications, and scientific strategies. Worked in the development of different in- vitro and in- vivo assays and experimental models to conduct the preclinical studies for oncology and dermatology research. Studied the self-renewal and differentiation mechanism of hematopoietic stem cells by using various transgenic mouse models. I used multicolor flow cytometry, cell sorting, bone marrow transplantation, animal cell culture and various molecular biology techniques to achieve my objectives. Got excellent experience in mice colony maintenance and setting up different breeding strategies. I have worked on target-based cancer drug discovery projects. I have established different in-vitro bioassays for the screening of protein-specific modulators. I have high experience in various techniques like animal cell culture and cell line maintenance, Expression and purification of proteins, Fluorescent microscopy, Protein based screening of small molecules, Screening of small molecules in 2D and 3D cell culture and their mechanism of action by FACS, si-RNA transfection and various other cell viability assays. I also have experience in the generation of in-vivo breast cancer and skin cancer model for study of anticancer activity of compounds. Besides the benchwork, I have experience in lab establishment and maintenance. I have excellent ability in data analysis, organization, scientific literature writing, and publication, perform collaborative work with different expertise and project leadership.

Subject Matter Expertise

• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Apoptosis
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ PCR
• ☆ Plasmid DNA
• ☆ DNA Damage & Repair
• ☆ Cell Biology
• ☆ Stem Cell Research
• ☆ Biomarker Discovery
• ☆ Blotting
• ☆ Drug Discovery
• ☆ Drug Design
• ☆ Drug Development
• ☆ Small Molecule Drug Development
• ☆ Preclinical Research
• ☆ Preclinical Pharmacology
• ☆ Immunotherapy
• ☆ Cancer Research
• ☆ Cancer Biology

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Non-Medical Regulatory Writing, Technical Writing, Newswriting, General Proofreading & Editing, Translation

Research Gray Literature Search, Scientific and Technical Research, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Image Analysis

Product Development Concept Development

Work Experience

Senior Scientist

SAI Life Sciences Ltd

February 2024 - Present

Manager

Sun Pharma Advanced Research Company Ltd

August 2021 - Present

Research Associate

University of Wisconsin–Madison

January 2019 - July 2021

Research Associate

University of Wisconsin–Madison

December 2017 - January 2019

Education

Ph.D

CSIR-Central Drug Research Institute Molecular and Structural Biology Division

January 2012 - September 2017

M.Sc.Biotechnology

BBA University Lucknow- India

July 2008 - June 2010

Certifications

• Initiating and planning projects

  University of California Irvine on Courera

  January 2023 - Present

Publications

JOURNAL ARTICLE

Anna L. F. V. Assump{\c{c}}{\~{a}}o and Guoping Fu and Deependra K. Singh and Zhanping Lu and Ashley M. Kuehnl and Rene Welch and Irene M. Ong and Renren Wen and Xuan Pan(2021). A lineage-specific requirement for YY1 Polycomb Group protein function in early T cell development . Development. 148. (7). The Company of Biologists

(2018). Multiple Machine Learning Based-Chemoinformatics Models for Identification of Histone Acetyl Transferase Inhibitors . Molecular informatics.

(2018). IMPACT OF PHYTOHORMONES AUXIN AND CYTOKININ ON MAMMALIAN CELLS. International Journal of Bio-Technology and Research (IJBTR).

(2018). siRNA Delivery Using a Cationic-Lipid-Based Highly Selective Human DNA Ligase I Inhibitor . ACS applied materials & interfaces.

(2017). A Novel Benzocoumarin-Stilbene Hybrid as a DNA ligase I inhibitor with in vitro and in vivo anti-tumor activity in breast cancer models . Scientific reports.

(2017). Identification of human flap endonuclease 1 (FEN1) inhibitors using a machine learning based consensus virtual screening . Molecular bioSystems.

(2016). Identification of a novel human DNA ligase I inhibitor that promotes cellular apoptosis in DLD-1 cells: an in silico and in vitro mechanistic study . RSC advances 6 (97), 94574-94587.

(2016). Dynamics of replication proteins during lagging strand synthesis: A crossroads for genomic instability and cancer . DNA repair.

(2016). Discovery of monocarbonyl curcumin hybrids as a novel class of human DNA ligase I inhibitors: in silico design, synthesis and biology . RSC advances 6 (31), 26003-26018.

(2014). Discovery of 3-Arylcoumarin-tetracyclic Tacrine Hybrids as Multifunctional Agents against Parkinson's Disease . ACS medicinal chemistry letters.

(2014). Pharmacophore-based screening and identification of novel human ligase I inhibitors with potential anticancer activity . Journal of chemical information and modeling.

(2013). Human DNA ligases: a comprehensive new look for cancer therapy . Medicinal research reviews.

BOOK CHAPTER

(2021). Methods for detection and measurement of calcium in plants . Calcium Transport Elements in Plants 2021, Pages 411-426.

(2021). Assays and protocols for the detection of cancer metastasis. PROTOCOL HANDBOOK FOR CANCER BIOLOGY.

CONFERENCE PAPER

(2020). YY1 Promotes SCF/c-Kit Signaling and HSC Self-Renewal in Fetal Hematopoiesis. Blood (2020) 136 (Supplement 1): 27. 62 ASH, Annual meeting 2020.

PATENT

(2019). Cationic lipid cordiarimide hybrid compounds and a process for preparation thereof. US Patent.