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Hire Dr. Ana Palma T.

Switzerland

USD 50 /hr

Profile Summary

Nine years post-Ph.D. experience in quantitative analysis of mammalian cellular metabolism. Proven track record in using stable-isotope tracers and metabolic flux analysis to identify metabolic constraints during production of protein, gene- and stem cell-based therapies. Skilled in metabolomics with hands-on experience in NMR and MS analyses. Excellent teamwork, communication and writing skills developed by managing own research team to successfully accomplish multiple academic and industry projects. Published 37 peer-reviewed Articles (12 as corresponding author, 12 as first author) and 7 Book Chapters.

Subject Matter Expertise

• ★ Metabolism & Metabolic Disease
• ★ Research
• ★ Technical & Scientific Writing
• ☆ NMR Spectroscopy
• ☆ Animal Biotechnology
• ☆ Metabolomics
• ☆ Stem Cell Research
• ☆ Industrial Biotechnology
• ☆ Gene Therapy

Services

• Writing
• Consulting
• Data & AI
• Product Development

Work Experience

Visiting Scientist

ETH Zurich

January 2017 - Present

Principal Investigator

IBET - Instituto de Biologia Experimental e Tecnológica

January 2015 - Present

Lecturer in Master and PhD courses

NOVA - New University of Lisbon

January 2010 - June 2016

Senior Associate Researcher

ITQB - Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa

March 2009 - December 2014

Postdoc Fellow

IBET - Instituto de Biologia Experimental e Tecnológica

July 2008 - February 2009

Education

PhD in Biochemical Engineering (Faculty of Sciences and Technology)

Universidade Nova de Lisboa

October 2003 - June 2008

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2017). Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells . Scientific Reports. 7. (1).

(2017). Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production . Vaccine.

(2017). Quantification of Metabolic Rearrangements During Neural Stem Cells Differentiation into Astrocytes by Metabolic Flux Analysis . Neurochemical Research. 42. (1). p. 244-253.

(2017). Synthetic biology-inspired therapies for metabolic diseases . Current Opinion in Biotechnology. 47. p. 59-66.

(2016). Functional metabolic interactions of human neuron-astrocyte 3D in vitro networks . Scientific Reports. 6.

(2014). Towards real-time monitoring of therapeutic protein quality in mammalian cell processes . Current Opinion in Biotechnology. 30. p. 161-167.

(2014). Insect cell line development using FLP-mediated cassette exchange technology . Methods in molecular biology (Clifton, N.J.). 1104. p. 15-27.

(2014). Metabolic responses of CHO cells to limitation of key amino acids . Biotechnology and Bioengineering. 111. (10). p. 2095-2106.

(2013). A cell sorting protocol for selecting high-producing sub-populations of Sf9 and High Five™ cells . Journal of Biotechnology.

(2013). Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition . Biotechnology and Bioengineering. 110. (12). p. 3244-3257.

(2013). Insect cells as a production platform of complex virus-like particles . Expert Review of Vaccines. 12. (2). p. 225-236.

(2012). Systems biotechnology of animal cells: The road to prediction . Trends in Biotechnology. 30. (7). p. 377-385.

Carrondo, M.J.T., Alves, P.M., Carrondo, M.J.T., Alves, P.M., Carinhas, N., Glassey, J., Hesse, F., Merten, O.-W., Micheletti, M., Noll, T., et al.(2012). How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology? . Biotechnology Journal. 7. (12). p. 1522-1529.

(2011). Cell functional enviromics: Unravelling the function of environmental factors . BMC Systems Biology. 5.

(2011). A comprehensive metabolic profile of cultured astrocytes using isotopic transient metabolic flux analysis and 13C-labeled glucose . Frontiers in Neuroenergetics. (SEP).

(2011). Synchronous fluorescence spectroscopy as a novel tool to enable PAT applications in bioprocesses . Biotechnology and Bioengineering. 108. (8). p. 1852-1861.

(2011). Hybrid metabolic flux analysis: Combining stoichiometric and statistical constraints to model the formation of complex recombinant products . BMC Systems Biology. 5.

(2011). High-throughput analysis of animal cell cultures using two-dimensional fluorometry . Journal of Biotechnology. 151. (3). p. 255-260.

(2010). Metabolic alterations induced by ischemia in primary cultures of astrocytes: Merging 13C NMR spectroscopy and metabolic flux analysis . Journal of Neurochemistry. 113. (3). p. 735-748.

(2009). Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative . Biotechnology Advances. 27. (6). p. 726-732.

(2006). Hybrid metabolic flux analysis/data-driven modelling of bioprocesses . Computer Aided Chemical Engineering. 21. (C). p. 1667-1672.

(2005). Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems . Journal of Biotechnology. 118. (3). p. 290-303.

(2005). Hybrid metabolic flux analysis/artificial neural network modeling of bioprocesses . Proceedings - HIS 2005: Fifth International Conference on Hybrid Intelligent Systems. 2005. p. 411-416.

BOOK CHAPTER

Silva, A. C., Roldão, A., Teixeira, A., Fernandes, P., Sousa, M. F., & Alves, P. M. (2015). Cell immobilization for the production of viral vaccines. In Animal cell culture (pp. 541-563). Springer International Publishing .

BOOK

(2014). Metabolic flux analysis tools to investigate brain metabolism in vitro . Neuromethods. 90. p. 107-144.

(2014). H-NMR protocol for exometabolome analysis of cultured mammalian cells . Methods in Molecular Biology. 1104. p. 237-247.

(2014). Insect cell line development using flp-mediated cassette exchange technology . Methods in Molecular Biology. 1104. p. 15-27.

(2011). Online Control Strategies . Comprehensive Biotechnology, Second Edition. 2. p. 875-882.