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USD 150 /hr
Hire Dr. Alexey P.
United States
USD 150 /hr

Bioinformatics expert specializing in protein interactions, structure modeling, and protein design

Profile Summary
Subject Matter Expertise
Services
Data & AI Predictive Modeling, Statistical Analysis, Algorithm Design-ML, Data Visualization, Data Mining, Data Insights
Work Experience

Associate Professor

Cincinnati Children's Hospital Medical Center

October 2017 - Present

Associate Professor

Cincinnati Children's Hospital Medical Center

October 2017 - Present

ASSISTANT PROFESSOR

Cincinnati Children's Hospital Medical Center

February 2013 - September 2017

ASSISTANT PROFESSOR

University of Cincinnati

November 2006 - January 2013

Post-doctoral Fellow

Cincinnati Children's Hospital Medical Center

May 2002 - October 2006

Education

PhD (Chemistry)

Tver State University - Russian Federation

September 1995 - June 1999

Certifications
  • Certification details not provided.
Publications
PREPRINT
Aaron W. Albee, Steven G. Sayson, Alan Ashbaugh, Aleksey Porollo, George Smulian, Melanie T. Cushion (2025). Single-Cell RNA Sequencing Defines Developmental Progression and Reproductive Transitions ofPneumocystis carinii .
Steven G. Sayson, Alan Ashbaugh, Aleksey Porollo, Melanie T. Cushion (2024). Pneumocystis murinaPromotes Inflammasome Formation and NETosis duringPneumocystisPneumonia .
JOURNAL ARTICLE
Steven G. Sayson, Alan Ashbaugh, Aleksey Porollo, George Smulian, Melanie T. Cushion, Kirsten Nielsen, Jay Kolls (2024). Pneumocystis murina promotes inflammasome formation and NETosis during Pneumocystis pneumonia . mBio.
Aleksey Porollo, Steven G. Sayson, Alan Ashbaugh, Sandra Rebholz, Julio A. Landero Figueroa, Melanie T. Cushion (2024). Insights into copper sensing and tolerance in Pneumocystis species . Frontiers in Microbiology.
Chau Tran, Siddharth Khadkikar, Aleksey Porollo (2023). Survey of Protein Sequence Embedding Models . International Journal of Molecular Sciences.
Alexey Porollo, Chau Tran, Siddharth Khadkikar (2023). Survey of Protein Sequence Embedding Models . International Journal of Molecular Sciences.
Tran, C., Khadkikar, S., Porollo, A.(2023). Survey of Protein Sequence Embedding Models . International Journal of Molecular Sciences. 24. (4).
Elliott, K.H., Balchand, S.K., Paese, C.L.B., Chang, C.-F., Yang, Y., Brown, K.M., Rasicci, D.T., He, H., Thorner, K., Chaturvedi, P., et al.(2023). Identification of a heterogeneous and dynamic ciliome during embryonic development and cell differentiation . Development (Cambridge). 150. (8).
Marsili, L., Davis, J.L., Espay, A.J., Gilthorpe, J., Williams, C., Kauffman, M.A., Porollo, A.(2023). SOD1-Related Cerebellar Ataxia and Motor Neuron Disease: Cp Variant as Functional Modifier? . Cerebellum.
Alexey Porollo, Nikeya Tisdale, Alan Ashbauad, Keeley Hendrix, Margaret S. Collins, Melanie T. Cushion (2022). The Effects of Sex and Strain on <i>Pneumocystis murina</i> Fungal Burdens in Mice . Journal of Fungi.
Pieter-Jan Van&#160;Camp, Aleksey Porollo (2022). SEQ2MGS: an effective tool for generating realistic artificial metagenomes from the existing sequencing data . NAR Genomics and Bioinformatics.
Van Camp, P.-J., Porollo, A.(2022). SEQ2MGS: An effective tool for generating realistic artificial metagenomes from the existing sequencing data . NAR Genomics and Bioinformatics. 4. (3).
Macioce-Tisdale, N.L., Ashbaugh, A., Hendrix, K., Collins, M.S., Porollo, A., Cushion, M.T.(2022). The Effects of Sex and Strain on Pneumocystis murina Fungal Burdens in Mice . Journal of Fungi. 8. (10).
Ray, S., Tillo, D., Assad, N., Ufot, A., Porollo, A., Durell, S.R., Vinson, C.(2022). Altering the Double-Stranded DNA Specificity of the bZIP Domain of Zta with Site-Directed Mutagenesis at N182 . ACS Omega. 7. (1). p. 129-139.
Brusilovsky, M., Rochman, M., Rochman, Y., Caldwell, J.M., Mack, L.E., Felton, J.M., Habel, J.E., Porollo, A., Pasare, C., Rothenberg, M.E.(2021). Environmental allergens trigger type 2 inflammation through ripoptosome activation . Nature Immunology. 22. (10). p. 1316-1326.
Chowdhury, D., Gardner, J.C., Satpati, A., Nookala, S., Mukundan, S., Porollo, A., Landero Figueroa, J.A., Subramanian Vignesh, K.(2021). Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity . Frontiers in Immunology. 12.
Cushion, M.T., Tisdale-Macioce, N., Sayson, S.G., Porollo, A.(2021). The Persistent Challenge of Pneumocystis Growth Outside the Mammalian Lung: Past and Future Approaches . Frontiers in Microbiology. 12.
Pieter-Jan Van Camp, David B. Haslam, Aleksey Porollo (2020). Bioinformatics Approaches to the Understanding of Molecular Mechanisms in Antimicrobial Resistance . International Journal of Molecular Sciences.
Alexey Porollo, Pieter-Jan Van Camp, David B. Haslam (2020). Bioinformatics Approaches to the Understanding of Molecular Mechanisms in Antimicrobial Resistance . International Journal of Molecular Sciences.
Van Camp, P.-J., Haslam, D.B., Porollo, A.(2020). Prediction of Antimicrobial Resistance in Gram-Negative Bacteria From Whole-Genome Sequencing Data . Frontiers in Microbiology. 11.
Van Camp, P.-J., Haslam, D.B., Porollo, A.(2020). Bioinformatics approaches to the understanding of molecular mechanisms in antimicrobial resistance . International Journal of Molecular Sciences. 21. (4).
Chowdhury, D., Alrefai, H., Landero Figueroa, J.A., Candor, K., Porollo, A., Fecher, R., Divanovic, S., Deepe, G.S., Subramanian Vignesh, K.(2019). Metallothionein 3 Controls the Phenotype and Metabolic Programming of Alternatively Activated Macrophages . Cell Reports. 27. (13). p. 3873-3886.e7.
Ray, S., Ufot, A., Assad, N., Singh, J., Durell, S.R., Porollo, A., Tillo, D., Vinson, C.(2019). The bZIP mutant CEBPB (V285A) has sequence specific DNA binding propensities similar to CREB1 . Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 1862. (4). p. 486-492.
Pradhan, A., Dunn, A., Ustiyan, V., Bolte, C., Wang, G., Whitsett, J.A., Zhang, Y., Porollo, A., Hu, Y.-C., Xiao, R., et al.(2019). The S52F FOXF1 mutation inhibits STAT3 signaling and causes alveolar capillary dysplasia . American Journal of Respiratory and Critical Care Medicine. 200. (8). p. 1045-1056.
Melanie T. Cushion, Alan Ashbaugh, Keeley Hendrix, Michael J. Linke, Nikeya Tisdale, Steven G. Sayson, Aleksey Porollo(2018). Gene Expression of Pneumocystis murina after Treatment with Anidulafungin Results in Strong Signals for Sexual Reproduction, Cell Wall Integrity, and Cell Cycle Arrest, Indicating a Requirement for Ascus Formation for Proliferation . Antimicrobial Agents and Chemotherapy. 62. (5). American Society for Microbiology
Lombardo, R.C., Porollo, A., Cnota, J.F., Hopkin, R.J.(2018). Congenital heart disease and aortic arch variants associated with mutation in PHOX2B . Genetics in Medicine. 20. (12). p. 1538-1543.
Ray, S., Tillo, D., Assad, N., Ufot, A., Deppmann, C., Durell, S.R., Porollo, A., Vinson, C.(2018). Replacing C189 in the bZIP domain of Zta with S, T, V, or A changes DNA binding specificity to four types of double-stranded DNA . Biochemical and Biophysical Research Communications. 501. (4). p. 905-912.
Miller, M.L., Porollo, A., Wert, S.(2018). Ultrastructure of Highly Ordered Granules in Alveolar Type II Cells in Several Species . Anatomical Record. 301. (7). p. 1290-1302.
Cushion, M.T., Limper, A.H., Porollo, A., Saper, V.E., Sinai, A.P., Weiss, L.M.(2018). The 14th International Workshops on Opportunistic Protists (IWOP 14) . Journal of Eukaryotic Microbiology. 65. (6). p. 934-939.
Jeremy W. Cox, Richard A. Ballweg, Diana H. Taft, Prakash Velayutham, David B. Haslam, Aleksey Porollo(2017). A fast and robust protocol for metataxonomic analysis using RNAseq data . Microbiome. 5. (1). Springer Science and Business Media {LLC}
Cox, J.W., Ballweg, R.A., Taft, D.H., Velayutham, P., Haslam, D.B., Porollo, A.(2017). A fast and robust protocol for metataxonomic analysis using RNAseq data . Microbiome. 5. (1).
Caldwell, J.M., Collins, M.H., Kemme, K.A., Sherrill, J.D., Wen, T., Rochman, M., Stucke, E.M., Amin, L., Tai, H., Putnam, P.E., et al.(2017). Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation . Mucosal Immunology. 10. (5). p. 1190-1201.
Litosh, V.A., Rochman, M., Rymer, J.K., Porollo, A., Kottyan, L.C., Rothenberg, M.E.(2017). Calpain-14 and its association with eosinophilic esophagitis . Journal of Allergy and Clinical Immunology. 139. (6). p. 1762-1771.e7.
Tillo, D., Ray, S., Syed, K.-S., Gaylor, M.R., He, X., Wang, J., Assad, N., Durell, S.R., Porollo, A., Weirauch, M.T., et al.(2017). The Epstein-Barr Virus B-ZIP Protein Zta Recognizes Specific DNA Sequences Containing 5-Methylcytosine and 5-Hydroxymethylcytosine . Biochemistry. 56. (47). p. 6200-6210.
Frazier Baker, Melanie Cushion, Aleksey Porollo (2016). A Quantitative Model to Estimate Drug Resistance in Pathogens . Journal of Fungi.
Frazier N. Baker, Aleksey Porollo (2016). CoeViz: a web-based tool for coevolution analysis of protein residues . BMC Bioinformatics.
Baker, F.N., Porollo, A.(2016). CoeViz: A web-based tool for coevolution analysis of protein residues . BMC Bioinformatics. 17. (1).
Baker, F.N., Cushion, M.T., Porollo, A.(2016). A quantitative model to estimate drug resistance in pathogens . Journal of Fungi. 2. (4).
Cushion, M.T., Collins, M.S., Sesterhenn, T., Porollo, A., Vadukoot, A.K., Merinod, E.J.(2016). Functional characterization of Pneumocystis carinii inositol transporter 1 . mBio. 7. (6).
Subramanian Vignesh, K., Landero Figueroa, J.A., Porollo, A., Divanovic, S., Caruso, J.A., Deepe, G.S.(2016). IL-4 Induces Metallothionein 3- and SLC30A4-Dependent Increase in Intracellular Zn<sup>2+</sup> that Promotes Pathogen Persistence in Macrophages . Cell Reports. 16. (12). p. 3232-3246.
De Bruin, C., Mericq, V., Andrew, S.F., Van Duyvenvoorde, H.A., Verkaik, N.S., Losekoot, M., Porollo, A., Garcia, H., Kuang, Y., Hanson, D., et al.(2015). An XRCC4 splice mutation associated with severe short stature, gonadal failure, and early-onset metabolic syndrome . Journal of Clinical Endocrinology and Metabolism. 100. (5). p. E789-E798.
Porollo, A.(2014). EC2KEGG: A command line tool for comparison of metabolic pathways . Source Code for Biology and Medicine. 9. (1).
Namjou, B., Marsolo, K., Caroll, R.J., Denny, J.C., Ritchie, M.D., Verma, S.S., Lingren, T., Porollo, A., Cobb, B.L., Perry, C., et al.(2014). Phenome-wide association study (PheWAS) in EMR-linked pediatric cohorts, genetically links PLCL1 to speech language development and IL5-IL13 to Eosinophilic Esophagitis . Frontiers in Genetics. 5. (NOV).
Fechter, K., Porollo, A.(2014). MutaCYP: Classification of missense mutations in human cytochromes P450 . BMC Medical Genomics. 7. (1).
Syed, K., Porollo, A., Lam, Y.W., Grimmett, P.E., Yadav, J.S.(2013). CYP63A2, a catalytically versatile fungal P450 monooxygenase capable of oxidizing higher-molecular-weight polycyclic aromatic hydrocarbons, alkylphenols, and alkanes . Applied and Environmental Microbiology. 79. (8). p. 2692-2702.
Ma, L., Tao, Y., Duran, A., Llado, V., Galvez, A., Barger, J.F., Castilla, E.A., Chen, J., Yajima, T., Porollo, A., et al.(2013). Control of nutrient stress-induced metabolic reprogramming by PKCζ in tumorigenesis . Cell. 152. (3). p. 599-611.
Hu, P., Chen, X., Whitener, R.J., Boder, E.T., Jones, J.O., Porollo, A., Chen, J., Zhao, L.(2013). Effects of parabens on adipocyte differentiation . Toxicological Sciences. 131. (1). p. 56-70.
SubramanianVignesh, K., LanderoFigueroa, J.A., Porollo, A., Caruso, J.A., Deepe, G.S.(2013). Granulocyte macrophage-colony stimulating factor induced Zn sequestration enhances macrophage superoxide and limits intracellular pathogen survival . Immunity. 39. (4). p. 697-710.
Green, J.V., Orsborn, K.I., Zhang, M., Tan, Q.K.G., Greis, K.D., Porollo, A., Andes, D.R., Lu, J.L., Hostetter, M.K.(2013). Heparin-binding motifs and biofilm formation by candida albicans . Journal of Infectious Diseases. 208. (10). p. 1695-1704.
Syed, K., Porollo, A., Miller, D., Yadav, J.S.(2013). Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic aromatic hydrocarbons . Protein Engineering, Design and Selection. 26. (9). p. 553-557.
Vignesh, K.S., Landero Figueroa, J.A., Porollo, A., Caruso, J.A., Deepe Jr., G.S.(2013). Zinc Sequestration: Arming Phagocyte Defense against Fungal Attack . PLoS Pathogens. 9. (12). p. 1-5.
Porollo, A., Meller, J., Joshi, Y., Jaiswal, V., Smulian, A.G., Cushion, M.T.(2012). Analysis of current antifungal agents and their targets within the pneumocystis carinii genome . Current Drug Targets. 13. (12). p. 1575-1585.
Ren, K., Lu, J., Porollo, A., Du, C.(2012). Tumor-suppressing function of caspase-2 requires catalytic site Cys-320 and site Ser-139 in mice . Journal of Biological Chemistry. 287. (18). p. 14792-14802.
Duran, A., Amanchy, R., Linares, J.F., Joshi, J., Abu-Baker, S., Porollo, A., Hansen, M., Moscat, J., Diaz-Meco, M.T.(2011). P62 Is a Key Regulator of Nutrient Sensing in the mTORC1 Pathway . Molecular Cell. 44. (1). p. 134-146.
Giridhar, P.V., Funk, H.M., Gallo, C.A., Porollo, A., Mercer, C.A., Plas, D.R., Drew, A.F.(2011). Interleukin-6 receptor enhances early colonization of the murine omentum by upregulation of a mannose family receptor, LY75, in ovarian tumor cells . Clinical and Experimental Metastasis. 28. (8). p. 887-897.
Biesiada, J., Porollo, A., Velayutham, P., Kouril, M., Meller, J.(2011). Survey of public domain software for docking simulations and virtual screening . Human Genomics. 5. (5). p. 497-505.
Lu, G., Haider, H.K., Porollo, A., Ashraf, M.(2010). Mitochondria-specific transgenic overexpression of connexin-43 simulates preconditioning-induced cytoprotection of stem cells . Cardiovascular Research. 88. (2). p. 277-286.
Porollo, A., Meller, J.(2010). POLYVIEW-MM: Web-based platform for animation and analysis of molecular simulations . Nucleic Acids Research. 38. (SUPPL. 2).
Korolev, V.L., Pivina, T.S., Porollo, A.A., Petukhova, T.V., Sheremetev, A.B., Ivshin, V.P.(2009). Differentiation of the molecular structure of nitro compounds as the basis for simulation of their thermal destruction processes . Russian Chemical Reviews. 78. (10). p. 945-969.
Porollo, A., Meller, J.(2007). Prediction-based fingerprints of protein-protein interactions . Proteins: Structure, Function and Genetics. 66. (3). p. 630-645.
Cushion, M.T., Smulian, A.G., Slaven, B.E., Sesterhenn, T., Arnold, J., Staben, C., Porollo, A., Adamczak, R., Meller, J.(2007). Transcriptome of Pneumocystis carinii during fulminate infection: Carbohydrate metabolism and the concept ofa compatible parasite . PLoS ONE. 2. (5).
Slaven, B.E., Meller, J., Porollo, A., Sesterhenn, T., Smulian, A.G., Cushion, M.T.(2006). Draft assembly and annotation of the Pneumocystis carinii genome . Journal of Eukaryotic Microbiology. 53. (SUPPL. 1).
Cao, B., Porollo, A., Adamczak, R., Jarrell, M., Meller, J.(2006). Enhanced recognition of protein transmembrane domains with prediction-based structural profiles . Bioinformatics. 22. (3). p. 303-309.
Korolev, V.L., Petukhova, T.V., Pivina, T.S., Porollo, A.A., Sheremetev, A.B., Suponitskii, Ku.Y., Ivshin, V.P.(2006). Thermal decomposition mechanisms of nitro-1,2,4-triazoles: A theoretical study . Russian Chemical Bulletin. 55. (8). p. 1388-1410.
Slaven, B.E., Porollo, A., Sesterhenn, T., Smulian, A.G., Cushion, M.T., Meller, J.(2006). Large-scale characterization of introns in the Pneumocystis carinii genome . Journal of Eukaryotic Microbiology. 53. (SUPPL. 1).
Adamczak, R., Porollo, A., Meller, J.(2005). Combining prediction of secondary structure and solvent accessibility in proteins . Proteins: Structure, Function and Genetics. 59. (3). p. 467-475.
Wagner, M., Adamczak, R., Porollo, A., Meller, J.(2005). Linear regression models for solvent accessibility prediction in proteins . Journal of Computational Biology. 12. (3). p. 355-369.
Porollo, A.A., Adamczak, R., Meller, J.(2004). POLYVIEW: A flexible visualization tool for structural and functional annotations of proteins . Bioinformatics. 20. (15). p. 2460-2462.
Adamczak, R., Porollo, A., Meller, J.(2004). Accurate prediction of solvent accessibility using neural networks-based regression . Proteins: Structure, Function and Genetics. 56. (4). p. 753-767.
Pivina, T.S., Sokerina, E.V., Lushnikov, D.E., Porollo, A.A., Ivshin, V.P.(1999). Computer modeling of probable decomposition reactions: cyclonitramines. Propellants, Explosives, Pyrotechnics. 24. (2). p. 99-107.
Porollo, A.A., Lushnikov, D.E., Pivina, T.S., Ivshin, V.P., Zefirov, N.S.(1999). Computer simulation of thermal decomposition reactions of alkyl nitrates . Russian Chemical Bulletin. 48. (10). p. 1845-1857.
Porollo, A.A., Lushnikov, D.E., Pivina, T.S., Ivshin, V.P.(1997). Computer representation and generation of possible pathways for thermal decomposition reactions of organic compounds . Journal of Molecular Structure: THEOCHEM. 391. (1-2). p. 117-124.
OTHER
Marsili, L., Davis, J.L., Espay, A.J., Gilthorpe, J., Williams, C., Kauffman, M.A., Porollo, A.(2022). SOD1-related cerebellar ataxia and motor neuron disease: A Cp modifier? . Research Square.
Chowdhury, D., Gardner, J.C., Satpati, A., Nookala, S., Mukundan, S., Porollo, A., Landero Figueroa, J.A., Vignesh, K.S.(2021). Metallothionein 3-zinc axis suppresses caspase-11 inflammasome activation and impairs antibacterial immunity . bioRxiv.
Ojha, M., Smith, N., Joshi, R., Goodman, E., Fan, Q., Schuman, R., Porollo, A., Zemans, R.L., Batie, M.R., Borchers, M.T., et al.(2020). Anti-CELA1 KF4 Antibody Prevents Emphysema by Inhibiting Stretch-Mediated Remodeling . medRxiv.
Tisdale, N.L., Ashbaugh, A., Hendrix, K., Collins, M.S., Porollo, A.P., Cushion, M.T.(2019). The effects of sex and strain on Pneumocystis murina fungal burdens in mice . bioRxiv.
BOOK CHAPTER
Porollo, A., Cushion, M.T.(2021). Advances in Genomics research of pneumocystis species . Encyclopedia of Mycology. p. 687-694.
BOOK
Biesiada, J., Porollo, A., Meller, J.(2012). On setting up and assessing docking simulations for virtual screening . Methods in Molecular Biology. 928. p. 1-16.
Swaminathan, K., Adamczak, R., Porollo, A., Meller, J.(2010). Enhanced prediction of conformational flexibility and phosphorylation in proteins . Advances in Experimental Medicine and Biology. 680. p. 307-319.