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Hire Sultan A.

United States

USD 50 /hr

Expert in cancer research using molecular biological and biochemical assay design and optimization

Profile Summary

PhD: Molecular and Cellular Pharmacology Expertise (with an emphasis on human tyrosine kinases): -Genetics (DNA cloning, primer design and optimization, indels, mutagenesis, RNA, RT-PCR, qPCR, dPCR, TaqMan probes, CRISPR gene editing (including prime editing, PASTE editing) -Immunoassays (Western blots, immunoprecipitation, co-immunoprecipitation, ELISA, chemiluminescence) -Recombinant Protein (expression and purification in bacteria, insect cells, and mammalian cells, affinity chromatography with a variety of affinity tags, IEX, SEC) -Biochemical Characterization (protein-protein interactions, in vitro assays using fluorescence, luminescence, and radioactivity readouts, kinetics parameters) -Cell-based Assays (mammalian cell culture, transient transfections, stable cell expression, transwell-migration) -Drug Screens (IC50 determination, high-throughput cell viability, drug synergism) Currently working on discovering novel therapeutics in NSCLC.

Subject Matter Expertise

• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Apoptosis
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ Genetics
• ☆ PCR
• ☆ Molecular Cloning
• ☆ DNA Sequencing
• ☆ Gene Expression Analysis
• ☆ Genome Sequencing
• ☆ Mutagenesis
• ☆ Recombinant DNA
• ☆ DNA/Gene Synthesis
• ☆ Plasmid DNA
• ☆ Kinases
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cytochemistry
• ☆ Cell Signaling Pathways
• ☆ Gene Regulation
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Fluorescence Microscopy
• ☆ Cell Culture
• ☆ Cell Line Engineering
• ☆ Cell Surface Receptors
• ☆ Biomolecular Engineering
• ☆ Gene Synthesis
• ☆ Genetic Engineering
• ☆ Gene Editing/CRISPR
• ☆ Protein/Enzyme Engineering
• ☆ Gene Delivery
• ☆ Blotting

Services

• Research
• Consulting

Research Scientific and Technical Research

Consulting Scientific and Technical Consulting

Work Experience

Postdoctoral Research fellow

University of Michigan

January 2024 - Present

Education

Molecular and Cellular Pharmacology

Stony Brook University

August 2017 - December 2022

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Krishnan H, Ahmed S, Hubbard SR, W. Todd Miller (2024). Catalytic activities of wild-type C. elegans DAF-2 kinase and dauer-associated mutants . The FEBS journal.

Sultan Ahmed, Harini Krishnan, Stevan R. Hubbard, W. Todd Miller (2024). Biochemical characterization of the Drosophila insulin receptor kinase and longevity‐associated mutants . The FASEB Journal.

Sultan Ahmed, Harini Krishnan, Stevan Hubbard, W. Todd Miller (2024). Biochemical characterization of the <i>Drosophila</i> insulin receptor kinase and longevity‐associated mutants . The FASEB Journal.

Yunyoung Kim, Ahmed S, Miller WT (2023). Colorectal cancer-associated mutations impair EphB1 kinase function . The Journal of biological chemistry.

Ahmed S, Miller WT (2022). The noncatalytic regions of the tyrosine kinase Tnk1 are important for activity and substrate specificity . The Journal of biological chemistry.