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USD 80 /hr

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Hire Dr. SIVAN KALYANI V.

India

USD 80 /hr

CRISPR gRNA design for indel, prime edit, base edit, HDR donor template, cloning module for multiplex edit, mutant seq

Profile Summary

Experienced researcher specializing in genome editing and molecular biology, currently working at the M S Swaminathan Research Foundation, India, my expertise lies in advancing CRISPR-based technologies for plant trait improvement and molecular innovation. Over the past six years, I have built significant technical expertise in designing and executing CRISPR-mediated gene editing experiments, including molecular cloning, plant transformation (Agrobacterium and biolistic methods), tissue culture, and advanced genomic analyses. During my tenure at Gyeongsang National University, Korea, I developed a CRISPR-Cas12a Gemini viral multi-replicon system that enhanced HDR-based genome editing efficiency in tomato, contributing to two US patents focused on small-molecule–mediated HDR improvements. At Iwate University, Japan, I employed CRISPR-Cas9 approaches to dissect gene functions in tomato development and stress responses, while my work in Agricultureal Research Organisation, Vulkani, Israel integrated transcriptomics and metabolomics to develop treatments that mitigate postharvest chilling injury in mango and avocado.

Subject Matter Expertise

• ★ Agronomy & Crop Science
• ★ Plant Biotechnology
• ★ Gene Editing/CRISPR
• ☆ Plant Physiology
• ☆ Phytochemistry
• ☆ Biochemistry
• ☆ Biomolecular Engineering

Services

• Research
• Consulting
• Data & AI

Research Technology Scouting, Scientific and Technical Research

Consulting Scientific and Technical Consulting, Regulatory Consulting

Data & AI Predictive Modeling, Image Processing, Image Analysis, Data Visualization

Work Experience

Co-Principal Investigator/ Senior Scientist

M S SWAMINATHAN RESEARCH FOUNDATION

November 2022 - Present

Specially Appointed Researcher

Iwate Daigaku

July 2019 - Present

Specially Appointed Researcher

Iwate University

June 2019 - December 2020

Research Professor

Gyeongsang National University

July 2016 - April 2018

Postdoctoral Researcher

Agricultural Research Organisation, VulKani Institute

January 2014 - December 2015

Education

Ph.D., in Biotechnology

Bharathiar University, Coimbatore, Tamil Nadu, India

January 2008 - December 2013

MSc in Biotechnology

Bharathiar University, Coimbatore, Tamil Nadu, India

June 2004 - July 2006

Bsc Biochemistry

Madurai Kamaraj University, Tamil Nadu, India

June 2001 - July 2004

Certifications

• CSIR-JRF cum NET, Govt of India All India Rank 089

  CSIR, Govt of India

  June 2011 - June 2011

Publications

JOURNAL ARTICLE

Sivan Kalyani Velu, Bhavini Krishnan, Gayatri Venkataraman (2025). Realizing the yield potential of Narrow Leaf 1 (NAL1) in rice: The way forward . Plant Physiology and Biochemistry.

Tien Van Vu, Velu Sivankalyani, Eun‐Jung Kim, Duong Thi Hai Doan, Mil Thi Tran, Jihae Kim, Yeon Woo Sung, Minwoo Park, Yang Jae Kang, Jae‐Yean Kim(2020). Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato . Plant Biotechnology Journal. 18. (10). p. 2133--2143. Wiley

Sivankalyani, V., Maoz, I., Feygenberg, O., Maurer, D., Alkan, N.(2017). Chilling stress upregulates a-linolenic acid-oxidation pathway and induces volatiles of C6 and C9 aldehydes in mango fruit . Journal of Agricultural and Food Chemistry. 65. (3). p. 632-638.

Sivankalyani, V., Feygenberg, O., Diskin, S., Wright, B., Alkan, N.(2016). Increased anthocyanin and flavonoids in mango fruit peel are associated with cold and pathogen resistance . Postharvest Biology and Technology. 111. p. 132-139.

Sivankalyani, V., Sela, N., Feygenberg, O., Zemach, H., Maurer, D., Alkan, N.(2016). Transcriptome dynamics in mango fruit peel reveals mechanisms of chilling stress . Frontiers in Plant Science. 7. (OCTOBER2016).

Sivankalyani, V., Geetha, M., Subramanyam, K., Girija, S.(2015). Ectopic expression of Arabidopsis RCI2A gene contributes to cold tolerance in tomato . Transgenic Research. 24. (2). p. 237-251.

Sivankalyani, V., Feygenberg, O., Maorer, D., Zaaroor, M., Fallik, E., Alkan, N.(2015). Combined treatments reduce chilling injury and maintain fruit quality in avocado fruit during cold quarantine . PLoS ONE. 10. (10).

Sivankalyani, V., Takumi, S., Thangasamy, S., Ashakiran, K., Girija, S.(2014). Punctured-hypocotyl method for high-efficient transformation and adventitious shoot regeneration of tomato . Scientia Horticulturae. 165. p. 357-364.