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USD 20 /hr

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Hire Shafana S.

India

USD 20 /hr

Freelance Biotechnologist & Molecular Biology Researcher | Expert in CRISPR, PCR & Gene Expression Analysis

Profile Summary

I am a freelance biotechnologist and molecular biology researcher with hands-on experience in CRISPR-Cas9 genome editing, gene expression analysis, and plant stress physiology. I hold a Master’s degree in Biotechnology from Cochin University of Science and Technology (CGPA 9.11/10) and a Bachelor’s degree in Biomedical Sciences from Sri Ramachandra Institute of Higher Education and Research (CGPA 9.13/10). My core expertise lies in molecular cloning, plasmid and genomic DNA isolation, RNA extraction, cDNA synthesis, PCR (colony, plasmid, RT-PCR), primer and gRNA design, and gene silencing techniques. I have designed and constructed CRISPR/Cas9 cassettes to silence drought-responsive genes in rice and conducted gene expression analysis to explore stress-induced gene behavior under abiotic conditions. I am also skilled in bioinformatics tools including BLAST, molecular docking (AutoDock, PyRx), phylogenetic analysis, and in silico gRNA validation. My research has been published in a peer-reviewed journal, focusing on CRISPR gRNA design for drought tolerance in rice. I’ve completed internships at Kerala Agricultural University and the Kerala Police Academy, where I worked on DNA fingerprinting, PCR, and phytochemical analysis. My academic and research journey has shaped me into a detail-oriented scientist with strong analytical and technical writing skills. I am passionate about applying molecular biology and computational tools to solve real-world biological problems. I am open to collaborations in molecular biology, genetic engineering, CRISPR-based projects, bioinformatics analyses, and academic writing.

Subject Matter Expertise

• ☆ Developmental Biology
• ☆ Embryology
• ☆ Computational Biology
• ☆ Evolutionary Biology
• ☆ Botany
• ☆ Phytochemistry
• ☆ Zoology
• ☆ Animal Science
• ☆ Animal Biotechnology
• ☆ Animal Welfare
• ☆ Animal Breeding
• ☆ Animal Genetics
• ☆ Mammalian Biology
• ☆ Molecular Biology
• ☆ Biomarkers
• ☆ Genetics
• ☆ Microarrays
• ☆ Molecular Evolution
• ☆ Chromatin
• ☆ Epigenetics
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Next Generation Sequencing (NGS)
• ☆ DNA Sequencing
• ☆ Synthetic Genomics
• ☆ Protein Sequencing
• ☆ Gene Expression Analysis
• ☆ Genome Sequencing
• ☆ Microinjection
• ☆ Mutagenesis
• ☆ Exome Sequencing
• ☆ Recombinant DNA
• ☆ RNA Synthesis
• ☆ RNA Sequencing
• ☆ DNA/Gene Synthesis
• ☆ Oligonucleotide Synthesis
• ☆ Plasmid DNA
• ☆ DNA Damage & Repair
• ☆ ChIP-Seq
• ☆ Kinases
• ☆ Monoclonal Antibody Production
• ☆ Molecular Ecology
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cytochemistry
• ☆ Cytogenetics
• ☆ Cell Signaling Pathways
• ☆ Membrane Biology
• ☆ Gene Regulation
• ☆ Stem Cell Research
• ☆ Basic Immunology
• ☆ Adjuvant & Antigen Research
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Bioprinting
• ☆ Microscopy
• ☆ Cryosectioning
• ☆ Light Microscopy
• ☆ Electron Microscopy
• ☆ Fluorescence Microscopy
• ☆ Scanning Probe Microscopy
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Cell Banking
• ☆ Cell Line Engineering
• ☆ Large Scale Cell Culture
• ☆ Cell Surface Receptors
• ☆ 3D Cell Culture
• ☆ Biosensors
• ☆ Blotting
• ☆ Immunohistochemistry
• ☆ Phylogenetics
• ☆ Population Genetics
• ☆ Circadian Rhythm Research
• ☆ Tissue Engineering
• ☆ Cytometry
• ☆ Cell Aging

Services

• Writing
• Research

Writing Medical Writing, Technical Writing, Business & Legal Writing, Copywriting, Creative Writing, Newswriting, General Proofreading & Editing, Translation

Research Scientific and Technical Research, Systematic Literature Review

Work Experience

Research trainee

Kerala agricultural University

September 2024 - February 2025

Education

B.Sc. (HONS) Biomedical Science

Sri Ramachandra Institute of Higher education and research

July 2018 - July 2024

M.Sc. Biotechnology

Cochin University of Science and Technology

July 2022 - May 2024

M.Sc. Biotechnology (Biotechnology)

Cochin University of Science and Technology

July 2022 - May 2024

B.Sc. (HONS) Biomedical Science (Biomedical Science)

Sri Ramachandra Institute of Higher Education and Research

July 2018 - July 2022

Certifications

• Genome Editing and Engineering

  IIT (Guwahati)

  October 2023 - Present

Publications

JOURNAL ARTICLE

Shafana S, Kripa Pappachan, Abida P.S, Acharya Arpita, G Bhavya, Melna Mary C.J (2024). In silico Design and Validation of CRISPR/Cas9 gRNAs for Enhancing Drought Tolerance and Yield in Rice . Journal of Advances in Biology & Biotechnology.