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Hire Dr. Sashi K.

Molecular biologist with 10+ year of experience in infection biology

Profile Summary

Currently investigating the molecular mechanisms by which Salmonella avoids encounter against reactive oxygen/nitrogen species and protects itself against oxidative and nitrosative stress. These investigations will aid in understanding novel ways Salmonella adapts to oxidative and nitrosative stress, an integral defense of innate immunity. Developing novel therapeutic approaches to target Salmonella and will also be broadly applicable to many key bacterial pathogens. Expertise in developing tissue specific knock-out mice. Expertise on genome engineering and editing for prokaryotic and eukaryotic cells. Conducted research on Sarcopenia (skeletal muscle loss), and played a key role in the identifying signaling mechanism of skeletal muscle loss in patients with alcoholic liver disease (ALD). Conducted research on Gram-positive bacterial pathogen (S. pyogenes, S. pneumoniae and S. aureus). Characterized Tyrosine kinase and Low molecular weight Tyrosine phosphatase in S. pyogenes that are linked to virulence, drug resistance, biofilm formation, bacterial cell division and growth. Provided first evidence for the presence of tyrosine phosphorylation in S. pyogenes. Identified a novel small molecule inhibitor (Inh2-B1) against Ser/Thr protein kinase (STK1) of S. aureus. STK1 plays a critical role in antibiotic resistance in methicillin-resistant S. aureus. By targeting STK1, Inh-B1 served as an “antibiotic resistance breaker”.

Subject Matter Expertise

• ☆ Microbiology
• ☆ Gram-Positive Bacteria
• ☆ Apoptosis
• ☆ Molecular Biology
• ☆ Kinases
• ☆ Pathogenesis
• ☆ Protein/Enzyme Engineering

Services

• Writing
• Data & AI

Work Experience

Instructor

University of Colorado Denver School of Medicine

April 2019 - Present

Post-doc

Cleveland Clinic Lerner Research Institute

April 2017 - March 2019

Post Doctoral Researcher

Ohio State University

March 2012 - March 2017

Education

Doctoral (Botany Department)

University of Delhi

April 2008 - September 2011

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2020). Activated protein phosphatase 2A disrupts nutrient sensing balance between mTORC1 and AMPK causing sarcopenia in alcoholic liver disease . Hepatology.

Sashi Kant, Saurabh Pandey, Bharat Yadav, Arvind Pandey, Takshashila Tripathi, Masuma Khawary, Deeksha Tripathi(2020). Lessons from {SARS}-{CoV}-2 Pandemic: Evolution, Disease Dynamics and Future . Biology. 9. (6). p. 141. {MDPI} {AG}

Sashi Kant, Saurabh Pandey, Bharat Yadav, Arvind Pandey, Takshashila Tripathi, masuma khawary, Deeksha Tripathi (2020). Lessons from SARS-CoV-2 Pandemic: Evolution, Disease Dynamics and Future . Biology.

(2020). Resistin in Metabolism, Inflammation and Disease . The FEBS Journal.

(2020). SpoT Induces Intracellular Salmonella Virulence Programs in the Phagosome . mBio.

(2019). Impaired Ribosomal Biogenesis by Non-Canonical Degradation of β-catenin during Hyperammonemia . Molecular and Cellular Biology.

Sashi Kant, Gangarao Davuluri, Khaled A. Alchirazi, Nicole Welch, Claire Heit, Avinash Kumar, Mahesha Gangadhariah, Adam Kim, Megan R. McMullen, Belinda Willard, et al.(2019). Ethanol sensitizes skeletal muscle to ammonia-induced molecular perturbations . Journal of Biological Chemistry. 294. (18). p. 7231--7244. American Society for Biochemistry {\&} Molecular Biology ({ASBMB})

(2017). A novel STK1-targeted small-molecule as an “antibiotic resistance breaker” against multidrug-resistant Staphylococcus aureus . Scientific Reports.

(2016). SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes . Front Cell Infect Microbiol.

(2015). The Streptococcus pyogenes orphan protein tyrosine phosphatase, SP-PTP, possesses the dual specificity and essential virulence regulatory functions .

A novel insecticidal GroEL protein from Xenorhabdus nematophila confers insect resistance in tobacco .

An insecticidal GroEL protein with chitin binding activity from Xenorhabdus nematophila .

Low expression levels of glnA1 accounts for absence of cell wall associated poly-L-glutamate/glutamine in Mycobacterium smegmatis .

A conserved hypothetical protein Rv0574c is required for cell wall integrity and virulence of Mycobacterium tuberculosis .

Evaluation of human LOX-12 as a serum marker for breast cancer .

Identification of a catabolite-responsive element necessary for regulation of the cry4A gene of Bacillus thuringiensis subsp. israelensis .

Surface plasmon resonance studies and biochemical evaluation of a potent peptide inhibitor against cyclooxygenase-2 as an anti-inflammatory agent .

BOOK CHAPTER

(2020). Bacterial Metabolic Fitness During Pathogenesis . Microbial Versatility in Varied Environments.