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Hire Dr. Roberto Z.

United Kingdom

Profile Summary

Research scientist with broad expertise in molecular and cell biology, and in the genetic engineering of animal models. In-depth knowledge of molecular and cell biology, especially with respect to the following areas: genetics, cell motility, membrane trafficking and host-pathogen interaction. Extensive experience in molecular cloning. Experience in pharmacokinetic modelling. Technical skills: - molecular cloning - confocal microscopy (live cell imaging and IF) - flow cytometry - protein purification - western, Southern and northern blotting - RT-qPCR - mammalian cell tissue culture - automated amino acid analysis - preparative scale HPLC - image analysis (ImageJ) and statistical analysis (R language) Research Projects Post-doctoral researcher, MRC Laboratory of Molecular Biology Dr Jason Chin’s group. Expanding the genetic code. Post-doctoral researcher, University of California, Irvine Investigating the role of intracellular transport during Chlamydia infection. Demonstrated that COPI but not COPII is required for the growth of the chlamydial inclusion. University of Cambridge, MRC Laboratory of Molecular Biology Ph.D. Project: The role of the endocytic cycle in the motility of the social amoeba Dictyostelium discoideum Demonstrated that the turnover of the plasma membrane is required to sustain cell motility. This was a new finding, which led research into an area of biology previously unconnected with cell motility. As part of my work, I produced small pieces of software that speeded up the analysis of data. I also improved existing experimental protocols and designed new assays. University College London BSc Research Project: Characterisation of mouse macrophage cell line IC-21 and its response to CSF-1 with respect to primary macrophages (Dr Anne Ridley’s group, Ludwig Institute for Cancer Research) Established that the IC-21 cell line is not suitable for cell motility studies.

Subject Matter Expertise

• ☆ Separation & Purification
• ☆ Liquid Chromatography/HPLC
• ☆ Biology/Life Sciences
• ☆ Microbiology
• ☆ Animal Biotechnology
• ☆ Animal Genetics
• ☆ Biochemistry
• ☆ Molecular Biology
• ☆ Basic Immunology
• ☆ Microscopy
• ☆ Genetic Engineering
• ☆ Protein/Enzyme Engineering
• ☆ Blotting
• ☆ Cytometry
• ☆ Experimental Protocols

Services

• Consulting
• Data & AI
• Product Development

Work Experience

Postdoctoral Fellow

MRC Laboratory of Molecular Biology

December 2011 - Present

Postdoctoral Fellow

University of California Irvine

January 2011 - December 2011

Education

Molecular and cell biology, Phd (MRC Laboratory of Molecular Biology)

UNIVERSITY OF CAMBRIDGE

October 2005 - September 2009

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Ernst, R.J., Krogager, T.P., Maywood, E.S., Zanchi, R., Beránek, V., Elliott, T.S., Barry, N.P., Hastings, M.H., Chin, J.W.(2016). Genetic code expansion in the mouse brain . Nature Chemical Biology.

Zanchi, R., Howard, G., Bretscher, M.S., Kay, R.R.(2010). The exocytic gene secA is required for Dictyostelium cell motility and osmoregulation . Journal of Cell Science. 123. (19). p. 3226-3234.