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Hire Dr. Pratik S.

Canada

USD 50 /hr

Biotechnology & AI Consultant - Accelerating Drug Discovery, Genomic Analysis, and Regulatory Strategy

Profile Summary

I am a molecular and cell biologist with a Ph.D. in Biological Sciences and more than eight years of academic and translational research experience spanning molecular biology, cell culture, and immunology. My research integrates advanced molecular assays with cellular and in vivo models to study host–pathogen interactions and evaluate therapeutic interventions. During my doctoral studies at CSIR–Central Drug Research Institute (affiliated with Jawaharlal Nehru University), I investigated molecular mechanisms governing liver-stage malaria infection. I developed and optimized RT-qPCR, western blotting, and immunofluorescence assays to quantify parasite gene expression and host responses. My work also included animal handling, drug dosing, tissue collection, and pharmacokinetic analyses to understand compound efficacy in murine malaria models. These studies deepened my understanding of molecular parasitology, immunobiology, and experimental therapeutics. As a Postdoctoral Research Associate at the University of Calgary, I expanded my expertise into mammalian cell culture and advanced imaging. I designed and executed complex in vitro experiments, maintained multiple mammalian and stem-cell lines, and employed fluorescence microscopy, ELISA, and flow cytometry to characterize immune signaling pathways. I have contributed to multiple high-impact publications and routinely collaborate across disciplines, combining microscopy, genomics, and bioinformatics to derive quantitative biological insights. My technical strengths include mammalian and primary cell culture, confocal imaging, RT-qPCR, protein assays, ELISA, flow cytometry, and western blotting. I am experienced in PK/PD studies, tissue biopsy, and sample processing, as well as in statistical analysis using GraphPad Prism, R, and Python. I have developed and maintained standard operating procedures (SOPs), managed laboratory inventories, and ensured GLP-style data documentation and safety compliance. Across all projects, I emphasize experimental rigor, reproducibility, and teamwork. I thrive in fast-paced, collaborative environments that combine molecular biology with translational goals. My ongoing research aims to integrate quantitative imaging and gene expression profiling to better understand host immune modulation and to support preclinical assay development for therapeutic discovery.

Subject Matter Expertise

• ☆ Computational Biology
• ☆ Biostatistics
• ☆ Molecular Biology
• ☆ Genetics
• ☆ Omics
• ☆ Transcriptomics
• ☆ Comparative Genomics
• ☆ Proteomics
• ☆ Genomics
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Synthetic Genomics
• ☆ Gene Expression Analysis
• ☆ Recombinant DNA
• ☆ RNA Sequencing
• ☆ Plasmid DNA
• ☆ DNA Damage & Repair
• ☆ Phylogenetics

Services

• Writing

Writing Technical Writing, General Proofreading & Editing

Work Experience

Postodoctoral Associate

University of Calgary

March 2022 - Present

Grad Student

CSIR-Central Drug Research Institute

January 2016 - January 2021

Education

Ph.D. (Molecular Parasitology and Immunology)

Central Drug Research Institute

January 2016 - September 2021

M. Tech. (Biotechnology)

Institute of Engineering and Technology

August 2011 - August 2014

B. Tech. (Biotechnology)

Sam Higginbottom University of Agriculture Technology and Sciences

August 2007 - July 2011

Certifications

• Certification details not provided.

Publications

PREPRINT

Ankit Ghosh, Akancha Mishra, Raksha Devi, Sunil Kumar Narwal, Nirdosh, Pratik Narain Srivastava, Satish Mishra (2024). A novel micronemal protein, Scot1, is essential for apicoplast biogenesis and liver stage development inPlasmodium berghei .

OTHER

Mishra, A., Srivastava, P.N., Ali, S.H., Mishra, S.(2023). Autophagy protein Atg7 is essential and druggable for maintaining malaria parasite cellular homeostasis and organelle biogenesis . bioRxiv.

JOURNAL ARTICLE

Hamza Abdullahi, S., Uzairu, A., Shallangwa, G.A., Uba, S., Umar, A.B., Srivastava, P.N.(2023). In-silico design of some pyrazolopyridine analogs as breast cancer inhibitors: QSAR modeling, molecular docking, MD simulation, and pharmacological features profiling . Journal of the Indian Chemical Society. 100. (8).

Srivastava, P.N., Nayak, B., Dewaker, V., Mishra, S.(2022). C‑Mannosyltransferase Is Essential for Malaria Transmission in Plasmodium berghei . ACS Infectious Diseases. 8. (6). p. 1116-1123.

Singh, S., Srivastava, P.N., Meena, A., Luqman, S.(2022). Dietary flavonoid narirutin as a prospective antagonist of oncogenic pri/pre-microRNAs . Phytotherapy Research. 36. (2). p. 963-983.

Srivastava, P.N., Mishra, S.(2022). Disrupting a Plasmodium berghei putative phospholipase impairs efficient egress of merosomes . International Journal for Parasitology. 52. (8). p. 547-558.

Dewaker, V., Srivastava, P.N., Verma, S., Srivastava, A.K., Prabhakar, Y.S.(2022). Non-bonding energy directed designing of HDAC2 inhibitors through molecular dynamics simulation . Journal of Biomolecular Structure and Dynamics. 40. (24). p. 13432-13455.

Srivastava, P.N., Narwal, S.K., Mishra, S.(2021). Mitochondrial apurinic/apyrimidinic endonuclease Apn1 is not critical for the completion of the Plasmodium berghei life cycle . DNA Repair. 101.

Dewaker, V., Srivastava, P.N., Verma, S., Prabhakar, Y.S.(2020). Molecular dynamics study of HDAC8-largazole analogues co-crystals for designing potential anticancer compounds . Journal of Biomolecular Structure and Dynamics. 38. (4). p. 1197-1213.

Gupta, R., Mishra, A., Choudhary, H.H., Narwal, S.K., Nayak, B., Srivastava, P.N., Mishra, S.(2020). Secreted protein with altered thrombospondin repeat (SPATR) is essential for asexual blood stages but not required for hepatocyte invasion by the malaria parasite Plasmodium berghei . Molecular Microbiology. 113. (2). p. 478-491.

Ravi Jillapalli, Sunil Kumar Narwal, Surendra Kumar Kolli, Babu S. Mastan, Rameswara Reddy Segireddy, Sandeep Dey, Pratik Narain Srivastava, Satish Mishra, Kota Arun Kumar(2019). A Plasmodium berghei putative serine-threonine kinase 2 (PBANKA_0311400) is required for late liver stage development and timely initiation of blood stage infection . Biology Open. 8. (8). p. bio042028. The Company of Biologists

Jillapalli, R., Narwal, S.K., Kolli, S.K., Mastan, B.S., Segireddy, R.R., Dey, S., Srivastava, P.N., Mishra, S., Kumar, K.A.(2019). A Plasmodium berghei putative serine-threonine kinase 2 (PBANKA_0311400) is required for late liver stage development and timely initiation of blood stage infection . Biology Open. 8. (8).

Choudhary, H.H., Srivastava, P.N., Singh, S., Kumar, K.A., Mishra, S.(2018). The shikimate pathway enzyme that generates chorismate is not required for the development of Plasmodium berghei in the mammalian host nor the mosquito vector . International Journal for Parasitology. 48. (3-4). p. 203-209.

Srivastava, P.N., Jain, R., Dubey, S.D., Bhatnagar, S., Ahmad, N.(2016). Prediction of Epitope-Based Peptides for Vaccine Development from Coat Proteins GP2 and VP24 of Ebola Virus Using Immunoinformatics . International Journal of Peptide Research and Therapeutics. 22. (1). p. 119-133.