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Hire Dr. Piyush K.

India

Profile Summary

Visiting Faculty (Genetics) at B.N.College, Patna University. PhD Work • During the course of my doctoral work, I have used chemical induced experimental carcinogenesis in the hamster buccal pouch (HBP) model. • HBP model is a commonly employed model in oral carcinogenesis experiments owing to similarities with human oral mucosa. • I have carried out sequential Raman spectroscopic evaluation of cancer progression and used histopathology and immunohistochemistry to corroborate the findings. • I have shown feasibility of Raman spectroscopy as a non-invasive, non-destructive and objective online tool suitable for preliminary screening/diagnosis. • I also showed that Raman can identify microheterogeneity in tissues which can be otherwise overlooked in conventional diagnostics. • Optical coherence tomography (OCT) imaging was also carried out to compare Raman and OCT findings. • Besides my doctoral work, I have also worked on bio fluids (blood, serum and urine), nanoparticles and curcumin in therapeutic evaluation, chalcones, reflexology, breast cancer models and forensic applications of Raman spectroscopy. Publications • I have published research articles related to oral and breast cancers and involving Raman spectroscopy and multivariate analysis in international peer reviewed journals. • I have also presented my work at international conferences, including SPEC 2014, supported by prestigious travel grants. Background life sciences/Genetics and early diagnostics in cancer biology; and experiences in spectroscopic/imaging tools, aided by extensive software based analysis; good writing and editing skills; an editorial member for a magazine 'Biotech Express'; have been involved in organizing seminars at my institute.

Subject Matter Expertise

• ☆ Analytical Chemistry
• ☆ Spectroscopy
• ☆ Raman Spectroscopy
• ☆ Biology/Life Sciences
• ☆ Multivariate Analysis
• ☆ Immunohistochemistry
• ☆ Oncology
• ☆ Histopathology
• ☆ Carcinogenesis

Services

• Writing
• Consulting
• Product Development

Work Experience

Icahn School of Medicine at Mount Sinai

- Present

Assistant Professor

Amity University

- Present

Visiting Faculty

B. N. College, Patna University

October 2016 - Present

Senior Research Fellow

ACTREC Tata Memorial Centre

November 2012 - March 2016

Junior Research Fellow

ACTREC Tata Memorial Centre

August 2010 - November 2012

Education

PhD

Advanced Centre for Treatment Research and Education in Cancer

August 2010 - January 2017

PhD

ACTREC Tata Memorial Centre

August 2010 - April 2016

M.Sc. Genetics

University of Delhi

June 2008 - May 2010

Master in Genetics (Department of Genetics)

University of Delhi

June 2008 - May 2010

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2021). Emerging role of cannabinoids and synthetic cannabinoid receptor 1/cannabinoid receptor 2 receptor agonists in cancer treatment and chemotherapy-associated cancer management . Journal of cancer research and therapeutics.

(2020). Advances in various techniques for isolation and purification of sterols . Journal of Food Science and Technology.

Piyush Kumar, Denice Peter Rout, Anushka Nair, Anand Gupta (2019). <p>Epidermolytic hyperkeratosis: clinical update</p> . Clinical, Cosmetic and Investigational Dermatology.

(2019). In vivo Raman spectroscopy: monitoring cancer progression post carcinogen withdrawal . Proc SPIE.

Piyush Kumar(2017). Raman spectroscopy as a promising noninvasive tool in brain cancer detection . Journal of Innovative Optical Health Sciences. 10. (05). p. 1730012. World Scientific Pub Co Pte Lt

Piyush Kumar, Tanmoy Bhattacharjee, Manishkumar Pandey, Arti Hole, Arvind Ingle, C. Murali Krishna(2016). Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues . Journal of Raman Spectroscopy. Wiley-Blackwell

(2016). • Evaluation of Raman spectroscopy for prediction of antitumor response to silibinin and its nanoparticulates in DMBA - induced oral carcinogenesis . Journal of Raman spectroscopy.

Gurushankar, K., Gohulkumar, M., Kumar, P., Murali Krishna, C., Krishnakumar, N.(2016). Raman spectroscopy detects biomolecular changes associated with nanoencapsulated hesperetin treatment in experimental oral carcinogenesis . Laser Physics Letters. 13. (3).

(2016). Raman spectroscopy in experimental oral carcinogenesis: investigation of abnormal changes in control tissues . Journal of Raman Spectroscopy.

Evaluation of Raman spectroscopy for prediction of antitumor response to silibinin and its nanoparticulates in DMBA-induced oral carcinogenesis @article{JRS:JRS4831, author= {Gohulkumar, M. and Kumar, Piyush and Murali Krishna, C. and Krishnakumar, N.}, title= {Evaluation of Raman spectroscopy for prediction of antitumor response to silibinin and its nanoparticulates in DMBA-induced oral carcinogenesis}, journal= {Journal of Raman Spectroscopy}, issn= {1097-4555}, url= {http://dx.doi.org/10.1002/jrs.4831}, doi= {10.1002/jrs.4831}, pages= {n/a--n/a}, keywords= {silibinin, nanoparticles, oral carcinogenesis, Raman spectroscopy, PC-LDA}, year= {2016}, abstract= {Raman spectroscopy is a vibrational spectroscopic technique that can be used to monitor the therapeutic efficacy of anticancer drugs during carcinogenesis in a non-invasive and label-free manner. The present study aims to investigate the biochemical changes exerted upon free silibinin (SIL) and its nanoparticulate (SILNPs) treatment against 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinogenesis in the fingerprint region of 1800–500 cm−1 using HE-785 Raman spectrometer. Raman spectra differed significantly between the control and tumor tissues, with tumor tissues characterized by increased intensities of vibrational bands such as nucleic acids, phenylalanine and tryptophan and a lower percentage of lipids when compared to the control tissues. Further, oral administration of free SIL and SILNPs significantly increased lipids and decreased the levels of tryptophan, phenylalanine and nucleic acid contents. Overall, the treatment of nanoparticulate SIL was found to be a more potent antitumor effect than free SIL in preventing the formation of tumor and also brought back the several Raman bands to a normal range in the buccal mucosa of hamsters during DMBA-induced oral carcinogenesis. In addition, the detailed secondary structure of proteins in the control and experimental groups is also presented. Furthermore, the diagnostic algorithms based on principal component linear discriminant analysis (PC-LDA) achieved an overall sensitivity of 94–100% and specificity of 76–100%. These results further demonstrate that Raman spectroscopy associated with PC-LDA diagnostic algorithms could be a valuable tool for rapid and sensitive detection of specific biomolecular changes at the molecular level in response to anticancer drug. Copyright © 2015 John Wiley & Sons, Ltd.}} . Journal of Raman Spectroscopy.

Raman spectroscopy detects biomolecular changes associated with nanoencapsulated hesperetin treatment in experimental oral carcinogenesis @article{1612-202X-13-3-035901, author= {K Gurushankar and M Gohulkumar and Piyush Kumar and C Murali Krishna and N Krishnakumar}, title= {Raman spectroscopy detects biomolecular changes associated with nanoencapsulated hesperetin treatment in experimental oral carcinogenesis}, journal= {Laser Physics Letters}, volume= {13}, number= {3}, pages= {035901}, url= {http://stacks.iop.org/1612-202X/13/i=3/a=035901}, year= {2016}, abstract= {Recently it has been shown that Raman spectroscopy possesses great potential in the investigation of biomolecular changes of tumor tissues with therapeutic drug response in a non-invasive and label-free manner. The present study is designed to investigate the antitumor effect of hespertin-loaded nanoparticles (HETNPs) relative to the efficacy of native hesperetin (HET) in modifying the biomolecular changes during 7,12-dimethyl benz(a)anthracene (DMBA)-induced oral carcinogenesis using a Raman spectroscopic technique. Significant differences in the intensity and shape of the Raman spectra between the control and the experimental tissues at 1800–500 cm −1 were observed. Tumor tissues are characterized by an increase in the relative amount of proteins, nucleic acids, tryptophan and phenylalanine and a decrease in the percentage of lipids when compared to the control tissues. Further, oral administration of HET and its nanoparticulates restored the status of the lipids and significantly decreased the levels of protein and nucleic acid content. Treatment with HETNPs showed a more potent antitumor effect than treatment with native HET, which resulted in an overall reduction in the intensity of several biochemical Raman bands in DMBA-induced oral carcinogenesis being observed. Principal component and linear discriminant analysis (PC–LDA), together with leave-one-out cross validation (LOOCV) on Raman spectra yielded diagnostic sensitivities of 100%, 80%, 91.6% and 65% and specificities of 100%, 65%, 60% and 55% for classification of control versus DMBA, DMBA versus DMBA  +  HET, DMBA versus DMBA  +  HETNPs and DMBA  +  HET versus DMBA  +  HETNPs treated tissue groups, respectively. These results further demonstrate that Raman spectroscopy associated with multivariate statistical algorithms could be a valuable tool for developing a comprehensive understanding of the process of biomolecular changes, and could reveal the signatures of the antitumor response of drugs.}} . Laser Physics Letters.

(2015). Raman spectroscopy detects biomolecular changes associated with nanoencapsulated hesperetin treatment in experimental oral carcinogenesis. Laser Physics Letters.

Bhattacharjee, T., Khan, A., Kumar, P., Ingle, A., Maru, G., Krishna, C.M.(2015). Raman spectroscopy of serum: A study on 'pre' and 'post' breast adenocarcinoma resection in rat models . Journal of Biophotonics. 8. (7). p. 575-583.

Kumar, P., Bhattacharjee, T., Ingle, A., Maru, G., Krishna, C. M.(2015). Raman Spectroscopy of Experimental Oral Carcinogenesis: Study on Sequential Cancer Progression in Hamster Buccal Pouch Model . Technol Cancer Res Treat.

Bhattacharjee, Tanmoy and Khan, Aarif and Kumar, Piyush and Ingle, Arvind and Maru, Girish and Krishna, C Murali(2014). Raman spectroscopy of serum: A study on ‘pre’and ‘post’breast adenocarcinoma resection in rat models. Journal of biophotonics. 9999. Wiley Online Library

(2014). Swiss bare mice: a suitable model for transcutaneous in vivo Raman spectroscopic studies of breast cancer .

Piyush Kumar, Krishna Dalal, D. Elanchezhiyan, V. B. Maran, Raunak Kumar Das, S. P. Singh, C. Murali Krishna, Jyotirmoy Chatterjee(2012). Optical, Spectroscopic, and Doppler Evaluation of “Normal” and “Abnormal” Reflexology Areas in Lumbar Vertebral Pathology: A Case Study . Case Reports in Medicine. 2012. p. 1--9. Hindawi Publishing Corporation

Piyush Kumar Optical, Spectroscopic and Doppler Evaluation of ‘normal’ and ‘abnormal’ Reflexology Areas in Lumbar Vertebral Pathology - a Case Study .

BOOK CHAPTER

(2019). Optical Techniques: Investigations in Oral Cancers . Oral Cancer Detection: Novel Strategies and Clinical Impact.

Panta, Prashanth and Lu, Chih-Wei and Kumar, Piyush and Ho, Tuan-Shu and Huang, Sheng-Lung and Kumar, Pawan and Murali Krishna, C. and Divakar Rao, K. and John, Renu(2019). Optical Coherence Tomography: Emerging In Vivo Optical Biopsy Technique for Oral Cancers . Oral Cancer Detection: Novel Strategies and Clinical Impact. p. 217--237. Springer International Publishing

Kumar, Piyush and Murali Krishna, C.(2019). Optical Techniques: Investigations in Oral Cancers . Oral Cancer Detection: Novel Strategies and Clinical Impact. p. 167--187. Springer International Publishing

(2016). Optical Techniques: Investigations in Oral Cancers. Oral Cancer Detection.

CONFERENCE PAPER

Bhattacharjee, T., Kumar, P., Fillipe, L.(2018). Automated pre-processing and multivariate vibrational spectra analysis software for rapid results in clinical settings . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 10489.

(2016). Raman spectroscopy of hamster buccal pouch model: Investigating suitability of ex vivo models to evaluate in vivo spectra. European Journal of Cancer.

MAGAZINE ARTICLE

(2017). Towards a smart era in biomedical diagnosis: Smartphone based hand held devices . Biotech Express.

(2015). Deadliest diseases of the world: Cancer. Biotech Express.