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Hire Dr. Pin-Yao H.

United States

USD 15 /hr

Biology PhD with 15yrs expertise in molecular genetics, genomics, CRISPR, and plant pathology

Profile Summary

Postdoctoral research • Research focuses on the organization of DNA into high-order structures in mouse embryonic stem cells, and how these structures impact the dynamic epigenetic landscape and transcriptional regulation during stem cell differentiation. • Apply genetic perturbation via CRISPR/Cas9 by creating genetic knockout or mutant lines, to assess the functional roles of different chromatin binding factors in chromatin organization. Design and conduct RNA-seq and ChIP-seq experiments and analyze the genomic data on a high-performance computing cluster. PhD research • Dissertation focused on applying a genetic screen for plant defense regulators against the grey mold disease and explored their potential in crop engineering. • Implemented gene transfer from Arabidopsis to enhance defense mechanisms in tomato and tobacco plants against pathogenic microbes.

Subject Matter Expertise

• ★ Plant Pathology
• ★ Molecular Biology
• ★ Epigenetics
• ☆ Genetics
• ☆ Chromatin
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Next Generation Sequencing (NGS)
• ☆ Genome Sequencing
• ☆ Recombinant DNA
• ☆ RNA Sequencing
• ☆ Plasmid DNA
• ☆ ChIP-Seq
• ☆ Cell Biology
• ☆ Gene Regulation
• ☆ Stem Cell Research
• ☆ Biotechnology & Bioengineering
• ☆ Cell Culture
• ☆ Agricultural Biotechnology
• ☆ Blotting

Services

• Research
• Consulting
• Data & AI

Research Fact Checking, Scientific and Technical Research

Consulting Scientific and Technical Consulting

Data & AI Image Processing, Data Visualization, Data Processing, Data Insights

Work Experience

Sr. Scientist

Ionis Pharmaceuticals

March 2023 - Present

Postdoctoral Fellow

NYU Langone Medical Center

November 2017 - March 2023

Education

PhD in Plant Biology

National Taiwan University

September 2012 - June 2017

Certifications

• Certification details not provided.

Publications

PREPRINT

Pin-Yao Huang, Havva Ortabozkoyun, Hyunwoo Cho, Aristotelis Tsirigos, Esteban Mazzoni, Danny Reinberg (2023). Novel Chromatin Insulating Activities Uncovered upon Eliminating Known Insulatorsin vivo .

Pin-Yao Huang, Havva Ortabozkoyun-Kara, Hyunwoo Cho, Varun Narendra, Gary Leroy, Jane A. Skok, Aristotelis Tsirigos, Esteban O. Mazzoni, Danny Reinberg(2020). A CRISPR Screen Identifies Myc-associated Zinc Finger Protein (MAZ) as an Insulator Functioning at CTCF boundaries in Hox Clusters . Cold Spring Harbor Laboratory

JOURNAL ARTICLE

Pin-Yao Huang, Havva Ortabozkoyun, Hyunwoo Cho, Varun Narendra, Gary LeRoy, Edgar Gonzalez-Buendia, Jane A. Skok, Aristotelis Tsirigos, Esteban O. Mazzoni, Danny Reinberg(2022). CRISPR and biochemical screens identify MAZ as a cofactor in CTCF-mediated insulation at Hox clusters . Nature Genetics. 54. (2). p. 202--212. Springer Science and Business Media {LLC}