Profile Details

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USD 11 /hr

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Hire Dr. Niyaz S.

Poland

USD 11 /hr

Protein purification expert, on demand protein / ligand molecular docking and virtual screening

Profile Summary

More than 20 years experience in Molecular biology / Biochemistry. Experience and skills include protein isolation and purification (from human, animal and plant tissues, as well as bacterial proteins) with all procedures and techniques associated (liquid chromatography, electrophoresis, enzyme kinetics etc.). Molecular cloning procedures including DNA analysis, pcr, site-directed mutagenesis, recombinant protein overexpression in bacterial cultures, IFA, ELISA etc. Special interest and skill is in the field of protein/ligand molecular docking and virtual screening to reveal natural (mostly plant) pharmaceutics to alter key enzymes activity. Protein 3D modeling and molecular graphics. I can perform detailed molecular docking studies and/or virtual screening for desired protein/ligand on demand 7 year experience as University Molecular Biology senior lecturer, Supervising of MSc and PHD students.

Subject Matter Expertise

• ★ Molecular Modeling
• ☆ Recombinant Protein Expression
• ☆ Experimental Protocols

Services

• Research
• Consulting
• Data & AI

Research Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis

Work Experience

Senior Lecturer, Lead Researcher

Baku State University

January 2014 - October 2017

Lead Researcher, Head

R & D Center for High Tech at the Ministry of Communications and Informational Technologies

July 2009 - September 2012

Lead Researcher

Azerbaijan National Academy of Sciences

November 2005 - July 2009

Senior Researcher

University of Bologna

September 2000 - September 2003

the department head deputy

Azerbaijan National Academy of Sciences Institute of Physiology

May 1998 - November 2001

Molecular Biology Dept. , Head

Azerbaijan National Academy of Sciences

April 1991 - May 1998

Senior Researcher, Molecular Modeling Lab, Head

"Biotech" R & D Association

February 1989 - April 1991

Senior Researcher

Azerbaijan National Academy of Sciences

June 1987 - February 1989

Junior researcher, Researcher, Senior Researcher

Azerbaijan National Academy of Sciences Institute of Physics

July 1976 - June 1987

Education

Senior Researcher in Biochemistry (Docent)

Higher Attestation Commitee at the Azerbaijan Ministry Chamber

July 1994 - July 1994

Ph.D in Biochemistry

Kiev State University

January 1983 - January 1983

MSc.; Biologist, Biology and Chemistry teacher (Biology/Biochemistry)

Azerbaijan State University

September 1971 - August 1976

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2020). Efficient matrix-assisted refolding of the recombinant anti-staphylococcal truncated endolysin LysKCA and its structural and enzymatic description . Protein Expression and Purification, Volume 174, October 2020, pages 105683,.

Niyaz Safarov and Ralphreed Gasanov(2015). Structural insights into β-glucuronidase inhibition to mitigate camptothecin chemoterapeutics side effects . International Journal of Pharmaceutical Chemistry. 5. (11). p. 375 - 380.

(2006). The Nickel Site ofBacilluspasteuriiUreE . Biochemistry.

(2006). The Nickel Site ofBacilluspasteuriiUreE, a Urease Metallo-Chaperone, As Revealed by Metal-Binding Studies and X-ray Absorption Spectroscopy† .

Stola, M., Musiani, F., Mangani, S., Turano, P., Safarov, N., Zambelli, B., Ciurli, S.(2006). The nickel site of Bacillus pasteurii UreE, a urease metallo-chaperone, as revealed by metal-binding studies and X-ray absorption spectroscopy . Biochemistry. 45. (20). p. 6495-6509.

Ciurli S, Safarov N, Miletti S, Dikiy A, Christensen SK, Kornetzky K, Bryant DA, Vandenberghe I, Devreese B, Samyn B, Remaut H, van Beeumen J, <i>Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry</i>, 2002, vol. 7, no. 6, pp. 623-631 .

Ciurli, S., Safarov, N., Miletti, S., Dikiy, A., Christensen, S.K., Kornetzky, K., Bryant, D.A., Vandenberghe, I., Devreese, B., Samyn, B., et al.(2002). Molecular characterization of Bacillus pasteurii UreE, a metal-binding chaperone for the assembly of the urease active site . Journal of Biological Inorganic Chemistry. 7. (6). p. 623-631.

Dikiy, A., Carpentier, W., Vandenberghe, I., Borsari, M., Safarov, N., Dikaya, E., Van Beeumen, J., Ciurli, S.(2002). Structural basis for the molecular properties of cytochrome c<inf>6</inf> . Biochemistry. 41. (50). p. 14689-14699.

(2002). Structural Basis for the Molecular Properties of Cytochromec6† . Biochemistry.

Bertini I, Bryant DA, Ciurli S, Dikiy A, Fernández CO, Luchinat C, Safarov N, Vila AJ, Zhao J, The Journal of biological chemistry, 2001, vol. 276, no. 50, pp. 47217-47226, 2001 .

Remaut H, Safarov N, Ciurli S, Van Beeumen J, The Journal of biological chemistry, 2001, vol. 276, no. 52, pp. 49365-49370, 2001 .

Remaut, H., Safarov, N., Ciurli, S., Van Beeumen, J.(2001). Structural Basis for Ni2+ Transport and Assembly of the Urease Active Site by the Metallochaperone UreE from Bacillus pasteurii . Journal of Biological Chemistry. 276. (52). p. 49365-49370.

Safarov, N, Miletti, S, Christensen, SK, Kornetzky, K, Vandenberghe, I, Devreese, B, Remaut, H, Van Beeumen, JJ, Bryant, DA, Ciurli, S(2001). Cloning, expression, purification, characterization, and structure determination of Bacillus pasteurii UreE, a putative Ni-chaperonin . Journal of Inorganic Biochemistry. 86. (1). p. 512.

(2001). The First Solution Structure of a Paramagnetic Copper(II) Protein:  The Case of Oxidized Plastocyanin from the CyanobacteriumSynechocystisPCC6803 . Journal of the American Chemical Society.

(2001). The First Solution Structure of a Paramagnetic Copper(II) Protein:  The Case of Oxidized Plastocyanin from the CyanobacteriumSynechocystisPCC6803 .

Bertini, I., Ciurli, S., Dikiy, A., Fern&#224;ndez, C.O., Luchinat, C., Safarov, N., Shumilin, S., Vila, A.J.(2001). The first solution structure of a paramagnetic copper(II) protein: The case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803 . Journal of the American Chemical Society. 123. (10). p. 2405-2413.

Bertini, I., Bryant, D.A., Ciurli, S., Dikiy, A., Fern&#225;ndez, C.O., Luchinat, C., Safarov, N., Vila, A.J., Zhao, J.(2001). Backbone dynamics of plastocyanin in both oxidation states: Solution structure of the reduced form and comparison with the oxidized state . Journal of Biological Chemistry. 276. (50). p. 47217-47226.

Bertini, I., Ciurli, S., Dikiy, A., Gasanov, R., Luchinat, C., Martini, G., Safarov, N.(1999). High-field NMR studies of oxidized blue copper proteins: The case of spinach plastocyanin . Journal of the American Chemical Society. 121. (10). p. 2037-2046.

Agalorv, R., Safarov, N., Gasanov, R.(1999). Properties of the docking site of photosystem 1 from spinach . Turkish Journal of Botany. 23. (5). p. 285-289.

Safarov, N.S., Baba-Zade, S.N., Akhmedli, K.M., Sadykhov, S.T.(1984). Mechanism of the phosphotransferase reaction catalyzed by guanosine-3',5'-monophosphate-dependent protein kinase | Izuchenie mekhanizma fosfotransferaznoǐ reaktsii, kataliziruemoǐ guanozin-3',5'-monofosfatzavisimoǐ proteinkinazoǐ . Ukrainskii biokhimicheskii zhurnal. 56. (1). p. 19-23.

Safarov NS, Baba-Zade SN, Akhmedli KM, Sadykhov ST, Ukrainskii biokhimicheskii zhurnal (1978), 1984, vol. 56, no. 1, pp. 19-23, 1984 .

Safarov NS, Musaev ZSh, Baba-Zade SN, Sadykhov ST, Mekhtiev NKh, Biokhimiia (Moscow, Russia), 1983, vol. 48, no. 6, pp. 991-997, 1983 .

Safarov, N.S., Musaev, Z.S., Baba-Zade, S.N., Sadykhov, S.T., Mekhtiev, N.K.(1983). Affinity chromatography and properties of cGMP-dependent protein kinase from prawn tissues | Affinnaia khromatografiia i svoǐstva cGMP-zavisimoǐ proteinkinazy iz tkaneǐ krevetki . Biokhimiya. 48. (6). p. 991-997.

Safarov NS, Musaev ZSh, Baba-Zade SN, Sadykhov ST, Mekhtiev NKh, Biokhimiia (Moscow, Russia), 1982, vol. 47, no. 6, pp. 945-949, 1982 .

Safarov, N.S., Musaev, Z.S., Baba-Zade, S.N., Sadykhov, S.T., Mekhtiev, N.K.(1982). Partial purification and properties of cGMP-dependent protein kinase from prawn tissue | Chastichnaia ochistka i izuchenie svoǐstv cGMP-zavisimoǐ proteinkinazy iz tkanei krevetki . Biokhimiya. 47. (6). p. 945-949.

MAGAZINE ARTICLE

(2012). Preparation, Some Properties of Porphyrin Nanotubes, and 2D Structural Modeling of Hybrid Porphyrin Monolayers.

(1999). High-Field NMR Studies of Oxidized Blue Copper Proteins:  The Case of Spinach Plastocyanin .

(1984). Two forms of cGMP-dependent protein kinase in prawn tissues. Dissociation of form 1.

CONFERENCE PAPER

Safarov N.S., Agalarov R.I., Isaev M.N., Dikiy A., Gasanov R.A.(1998). Isolation and spectroscopic characterization of cytochrome c6 from Cladophora glomerata . 1st Balkan Botanical Congress  Progress in Botanical Research. p. 235-238.