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Hire Nima G.

Brazil

USD 50 /hr

Enzyme engineering specialist with a PhD in Molecular Biology and Genetic Engineering, specializing in optimizing enzyme

Profile Summary

I hold a PhD in Molecular Biology and Genetic Engineering with specialized expertise in Enzymology and Enzyme Engineering. My research focuses on the rational design and optimization of industrially relevant enzymes, including PETase, phytase, lipase, and glucanase, to enhance their stability, activity, and overall performance. Through advanced protein engineering techniques such as site-directed mutagenesis, molecular docking, and ancestral sequence reconstruction, I have successfully improved the catalytic properties of these enzymes for diverse biotechnological applications. My comprehensive skill set includes both experimental and computational approaches, with a proven track record of delivering innovative solutions in enzyme development. (Google Scholar: https://scholar.google.com/citations?user=F5WfZ44AAAAJ&hl=en)

Subject Matter Expertise

• ☆ Molecular Modeling
• ☆ Molecular Docking
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Bioinformatics
• ☆ Gene & Protein Expression Analysis
• ☆ Biomolecular Engineering
• ☆ Genetic Engineering
• ☆ Protein/Enzyme Engineering

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing, Creative Writing

Research User Research, Feasibility Study, Gap Analysis, Gray Literature Search, Scientific and Technical Research, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Predictive Modeling, Image Analysis, Data Visualization, Text Mining & Analytics

Product Development Stability/Shelf Life Testing, Product Validation, Reverse Engineering

Work Experience

Postdoctoral Researcher

UKM

November 2024 - Present

Education

PhD

Universiti Putra Malaysia

September 2018 - April 2024

Certifications

• PhD

  Universiti Putra Malaysia

  April 2024 - Present

Publications

JOURNAL ARTICLE

Nima Ghahremani Nezhad, Samah Hashim Albayati, Anmar Ghanim Taki, Raja Noor Zaliha Raja Abd Rahman (2024). Efficient and easible biocatalysts: Strategies for enzyme improvement. A review . International Journal of Biological Macromolecules.

Nima Ghahremani Nezhad, Siti Zahra Binti Jamaludin, Raja Noor Zaliha Raja Abd Rahman, Normi Mohd Yahaya, Siti Nurbaya Oslan, Fairolniza Mohd Shariff, Nurulfiza Mat Isa, Thean Chor Leow (2024). Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae . World Journal of Microbiology and Biotechnology.

Nima Ghahremani Nezhad, Azadeh Eskandari, Thean Chor Leow, Mohd Basyaruddin Abdul Rahman, Siti Nurbaya Oslan (2024). Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli . Archives of Microbiology.

Nima Ghahremani Nezhad, Wan Atiqah Najiah Binti Wan Hasan, Mohd Adilin Yaacob, Abu Bakar Salleh, Raja Noor Zaliha Raja Abdul Rahman, Thean Chor Leow (2024). Shifting the pH profiles of Staphylococcus epidermidis lipase (SEL) and Staphylococcus hyicus lipase (SHL) through generating chimeric lipases by DNA shuffling strategy . World Journal of Microbiology and Biotechnology.

Nima Ghahremani Nezhad, Abdul Daim Mohammed Mukred, Raja Noor Zaliha Raja Abd Rahman, Mahiran Basri, Abu Bakar Salleh, Adam [Thean Chor] Leow (2024). Purification and biochemical characterization of extracellular thermostable lipase from Bacillus sp. strain L2 . Biologia.

Nima Ghahremani Nezhad, Azadeh Eskandari, Thean Chor Leow, Mohd Basyaruddin Abdul Rahman, Siti Nurbaya Oslan (2024). Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system . World Journal of Microbiology and Biotechnology.

Nima Ghahremani Nezhad, Sunusi Bataiya Buhari, Yahaya M. Normi, Fairolniza Mohd Shariff, Adam [Thean Chor] Leow (2024). Insight on recently discovered PET polyester-degrading enzymes, thermostability and activity analyses . 3 Biotech.

Yasotha a/p Sundaraj | Faculty of Engineering & Life Sciences, Hasdianty Abdullah, Nima Nezhad, Afiq Adham Abd Rasib, Roohaida Othman, Kenneth Rodrigues, Suriana Sabri, Syarul Baharum (2023). Cloning, Expression and Functional Characterization of a Novel α-Humulene Synthase, Responsible for the Formation of Sesquiterpene in Agarwood Originating from Aquilaria malaccensis . Current Issues in Molecular Biology.

Nima Ghahremani Nezhad, Fatin Liyana Alias, Yahaya M. Normi, Mohd Shukuri Mohamad Ali, Cahyo Budiman, Adam [Thean Chor] Leow (2023). Recent Advances in Overexpression of Functional Recombinant Lipases . Molecular Biotechnology.

Nima Ghahremani Nezhad, Raja Noor Zaliha Raja Abd Rahman, Yahaya M. Normi, Siti Nurbaya Oslan, Fairolniza Mohd Shariff, Adam [Thean Chor] Leow (2023). Isolation, screening and molecular characterization of phytase-producing microorganisms to discover the novel phytase . Biologia.

Nima Ghahremani Nezhad, Raja Noor Zaliha Raja Abd Rahman, Yahaya M. Normi, Siti Nurbaya Oslan, Fairolniza Mohd Shariff, Thean Chor Leow (2023). Recent advances in simultaneous thermostability-activity improvement of industrial enzymes through structure modification . International Journal of Biological Macromolecules.

Nima Ghahremani Nezhad, Siti Hajar Hamdan, Jonathan Maiangwa, Mohd Shukuri Mohamad Ali, Yahaya M. Normi, Fairolniza Mohd Shariff, Raja Noor Zaliha Raja Abd Rahman, Adam [Thean Chor] Leow (2023). Knotting terminal ends of mutant T1 lipase with disulfide bond improved structure rigidity and stability . Applied Microbiology and Biotechnology.

Nima Ghahremani Nezhad, Raja Noor Zaliha Raja Abd Rahman, Yahaya M. Normi, Siti Nurbaya Oslan, Fairolniza Mohd Shariff, Adam [Thean Chor] Leow (2022). Thermostability engineering of industrial enzymes through structure modification . Applied Microbiology and Biotechnology.

Nima Ghahremani Nezhad, Raja Noor Zaliha Raja Abd. Rahman, Yahaya M. Normi, Siti Nurbaya Oslan, Fairolniza Mohd Shariff, Adam [Thean Chor] Leow (2020). Integrative Structural and Computational Biology of Phytases for the Animal Feed Industry . Catalysts.