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USD 30 /hr

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Hire Dr. Nikhil B.

Singapore

USD 30 /hr

Expert in x-ray crystallography and cryo-EM | 10+ years of experience in structural biology

Profile Summary

Currently working at Nanyang Technological University as a Senior Research Fellow. My research work focuses on structural and functional studies of ligand-gated ion channels using cryo-Electron Microscopy. In past, I have worked on proteins involved in mitochondrial fusion in Ramachandran's laboratory at Case Western Reserve University. My Ph.D. research work involved the characterization of adenylyl cyclase proteins using X-ray crystallography. I have more than 10 years of experience in structural biology and I am working on cryo-EM for the past four years. I have 2 years of teaching experience. My skills involve protein expression and purification using bacteria and eukaryotic cell culture system, protein characterization using biochemical assays, CD, ITC, FRET, Microscale Thermophoresis, X-ray crystallography, Cryo-EM, etc. I have studied lipid and lipid membrane (liposome, nanodisc)protein interaction using MST and FRET and confocal microscopy. I have extensively used software used for x-ray crystallography and cryo-EM. I am an advanced Linux OS user. I have mentored several junior members of my current and past lab and I consider myself a highly motivated person who loves working in a team.

Subject Matter Expertise

• ☆ Analytical Chemistry
• ☆ Crystallography
• ☆ X-Ray Crystallography
• ☆ Microscopy
• ☆ Electron Microscopy
• ☆ Drug Discovery
• ☆ High Throughput Screening (HTS)
• ☆ Drug Target Identification
• ☆ Drug Design
• ☆ Structure-Activity Relationship (SAR) Studies
• ☆ Hypothesis
• ☆ Peer Review
• ☆ Research Methodology
• ☆ Survey Design & Methodology

Services

• Research
• Consulting
• Data & AI

Research Market Research, User Research, Meta-Research, Feasibility Study, Fact Checking, Gap Analysis, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Big Data Analytics

Work Experience

Senior Research Fellow

Nanyang Technological University

December 2021 - Present

Postdoctoral Researcher

Case Western Reserve University

September 2017 - October 2021

Postdoctoral Research Associate

Indian Institute of Science, Bengalore, India

July 2016 - February 2017

Assistant Professor

Modern College Of Arts, Science & Commerce

July 2008 - June 2010

Education

Postdoctoral Scholar (Department of Physiology and Biophysics)

Case Western Reserve University

September 2017 - Present

PhD (Molecular Biophysics Unit)

Indian Institute of Science

August 2010 - June 2016

M.Sc (Department of Microbiology)

Maharaja Sayajirao University of Baroda

August 2006 - July 2008

B. Sc. (Department of Microbiology)

HPT Arts and RYK Science College

August 2003 - July 2006

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Chang Yoon Doh and Nikhil Bharambe and Joshua B. Holmes and Katherine L. Dominic and Caitlin E. Swanberg and Ranganath Mamidi and Yinghua Chen and Smarajit Bandyopadhyay and Rajesh Ramachandran and Julian E. Stelzer(2022). Molecular characterization of linker and loop-mediated structural modulation and hinge motion in the C4-C5 domains of cMyBPC . Journal of Structural Biology. 214. (2). p. 107856. Elsevier {BV}

Nikhil Bharambe, Mukesh Mahajan, Yutong Shang, Bin Lu, Abhishek Mandal, Pooja Madan Mohan, Rihua Wang, Jennifer C. Boatz, Juan Manuel Martinez Galvez, Anna V. Shnyrova, et al.(2021). NMR identification of a conserved Drp1 cardiolipin-binding motif essential for stress-induced mitochondrial fission . Proceedings of the National Academy of Sciences. 118. (29). Proceedings of the National Academy of Sciences

Nikhil Bharambe, Jiayang Li, Ranganath Mamidi, Chang Yoon Doh, Joshua B. Holmes, Rajesh Ramachandran, Julian E. Stelzer(2020). AAV9 gene transfer of cMyBPC N-terminal domains ameliorates cardiomyopathy in cMyBPC-deficient mice . JCI Insight. American Society for Clinical Investigation

Bharambe, Nikhil G and Barathy, Deivanayaga V and Syed, Wajeed and Visweswariah, Sandhya S and Cola$\varsigma$o, Melwin and Misquith, Sandra and Suguna, Kaza(2016). Substrate specificity determinants of class III nucleotidyl cyclases. The FEBS journal. 283. (20). p. 3723--3738. Wiley Online Library

Satbhai, A and Kasodekar, A and Pachuau, L and Bharambe, N(2015). Isolation of halophiles from the Dead Sea and exploring their potential biotechnological applications. Int J Curr Microbiol Appl Sci. 2. p. 1--17.

Barathy, Deivanayaga V and Bharambe, Nikhil G and Syed, Wajeed and Zaveri, Anisha and Visweswariah, Sandhya S and Cola$\varsigma$o, Melwin and Misquith, Sandra and Suguna, Kaza(2015). Autoinhibitory mechanism and activity-related structural changes in a mycobacterial adenylyl cyclase. Journal of structural biology. 190. (3). p. 304--313. Elsevier

Akolkar, A and Bharambe, N and Trivedi, S and Desai, A(2009). Statistical optimization of medium components for extracellular protease production by an extreme haloarchaeon, Halobacterium sp. SP1 (1). Letters in applied microbiology. 48. (1). p. 77--83. Wiley Online Library

BOOK CHAPTER

Bharambe, Nikhil and Ramachandran, Rajesh(2020). Microscale Thermophoresis (MSTMicroscale thermophoresis (MST)) as a Tool for Measuring Dynamin Superfamily Protein (DSP)--Lipid Interactions . Dynamin Superfamily GTPases: Methods and Protocols. p. 85--92. Springer US