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Hire Dr. Nadia M.

Pakistan

USD 100 /hr

Assistant Professor (visiting faculty), PMAS Arid Agriculture University, Rawalpindi (Pak

Profile Summary

Awards and Honors 2007-2016 HEC indigenous PhD scholarship for MPhil leading to PhD 2012-2013 Visiting scientist fellowship by the Higher Education Commission (Pakistan); visited lab: Dr. David Douches, Michigan State University (U.S.A.) 2013 CSS451 “Biotechnology Applications for Plant Breeding and Genetics”, Michigan State University (U.S.A.) Selected Technical Skills Plant Tissue culturing Plant transformation: Agrobacterium-mediated stable and transient transformation, biolistic transformation (rice, potato, lentil) Cultivation of bacteria (Escherichia coli, Agrobacterium tumefaciens), chemical transformation and electroporation DNA and RNA extraction: plasmid DNA from Escherichia coli (DH5α) and Agrobacterium tumefaciens (GV3101, LBA4404, EHA105, C58C1), genomic DNA from potato, tobacco, rice, wheat, RNA extraction and handling PCR methods: standard PCR, RT-PCR for cDNA synthesis, qRT-PCR DNA cloning: “classical cloning” (restriction enzymes, ligase, phosphatase etc.), TA and Gateway cloning Protein expression, extraction and purification, subcellular fractionation and ultracentrifugation Gel electrophoresis: agarose (DNA and RNA), SDS-PAGE Spectrophotometry Thin layer chromatography (TLC) for analysis of plant pigments (chlorophyll and carotenes from spinach) CRISPR plasmid designing by Benchling CRISPR Design Tool, designing guide RNAs for gene knockout Use of bioinformatics tools e.g. BLAST, sequence alignments, primer design, gene constructs List of trained and supervised students 2013-2014 Zaigham Abbas Shah, PMAS Arid Agriculture University Rawalpindi (Master thesis research, now PhD student at PMAS Arid Agriculture University Rawalpindi) 2013-2015 Ifat Kiani, PMAS Arid Agriculture University Rawalpindi (Master thesis research, now working as a teacher at International Islamic University Islamabad College, Islamabad) Since 2018 Uzma Gul, Women University Mardan (Master thesis research) Teaching Experience Immunology and Immunochemistry Cell and Tissue Culture Applied Biochemistry General Biochemistry Biochemistry of Metabolism Carbohydrates Cellular Signaling Mechanism Biotechnology Introduction to Biochemistry Diversity of Plants Ecology, Biodiversity Evolution-I Ecology, Biodiversity Evolution II Human Physiology- I Mycology and Plant Pathology Macronutrients in Human Nutrition Classical Genetics Biochemistry-I Ecology, Biodiversity and Evolution-I Proteomics Plant biochemistry Protein chemistry General Virology Conferences, Seminars and Workshops Nation summer school on CRISPR-Cas9 genome editing, Codon Corps 2018 Community lab, Islamabad, Pakistan. National workshop on organic food and health, Avenues of innovation and entrepreneurship, University of Engineering and Technology, Peshawar- 2017 Pakistan Awareness Seminar on Technology Development Fund (TDF), “Establishment of Technology Development Fund for Academia and Industry Joint Venture Projects to Introduce New Technologies in Pakistan” 2017 University of Engineering and Technology, Peshawar-Pakistan 2016 th 4 International Conference on Biological and Computer Sciences, Capital University of Science and Technology, Islamabad-Pakistan 2014 nd 2 Conference on Emerging Trends in Bioinformatics and Computational Biology, M. A. J. U. Islamabad 2013 11 Biennial Conference on Molecular Biosciences-Challenges and Opportunities, University of the Punjab, Lahore-Pakistan 2013 “Bean Breeding at MSU and beyond”, seminar, Michigan State University, USA 2012 NCRP (North Central Regional Potato) Meeting. Holiday Inn Express Rosemont, IL, USA 2012 “Insight into the sugarcane genome’’, seminar at Michigan State University, USA 2012 Poster presentation in “South Asian Association for Regional Cooperation (SAARC) Regional Conference on New Frontiers in Agriculture Genomics and Biotechnology” held at Marriott Hotel Islamabad-Pakistan Poster title: “Genetic Transformation of OsRGLP1 into Potato” Authors: Nadia Majeed, Iqbal Hussain, S.M. Saqlan Naqvi 2011 International Workshop on Advances in Genetic Engineering of Plants at COMSTECH, Islamabad-Pakistan 2011 International Conference on Prospects and Challenges to Sustainable Agriculture in University of Azad Jammu and Kashmir, held at University of Azad Jammu and Kashmir, Rawalakot-Pakistan 2010 HEC/UAAR Workshop on Real Time PCR at the Department of Biochemistry PMAS AAUR, Islamabad-Pakistan 2008 9th Biennial PSBMB Conference on Advances in Biochemistry and Molecular Biology, PMAS AAUR, Islamabad-Pakistan Publications Deeba, F., Sultana, T., Majeed, N. and Naqvi, S.M.S. (2020) Heterologous expression of a plant WRKY protein confers multiple stress tolerance in E. coli. TURK J. BIOCHEM 45(2): 122-131 Majeed, N., Javaid, B., Deeba, F., Naqvi, S. M. S. and Douches, D. S. (2018) Enhanced Fusarium oxysporum f. sp. tuberosi resistance in transgenic potato expressing a rice GLP superoxide dismutase gene. Am J. Pot Res., 95(4): 383-394 Abbas, Z., Javaid B., Majeed, N. and Naqvi, S.M.S. (2016) Association of molecular markers with polyphenol oxidase activity in selected wheat genotypes. Pak. J. Bot., 48(2): 689-692. Sultana, T., Majeed, N., Khan, F., Rehman, A. U. and Naqvi, S.M.S. (2016) Direct regeneration and efficient in vitro root development studies in lentil (lens culinaris medik). Pak. J. Bot., 48(5): 19992004. Majeed, N., Hussain, I., Abbas, Z., Iqbal, U., Sultana, T. and Naqvi, S.M.S. (2016) Heterologous expression of OsRGLP1 gene induces resistance against Fusarium infection in transgenic potato. Pak. J. Phytopathol. 28: 45-55. Javaid, B., Majeed, N., Deeba, F., Ahmad, S. M., Rehman, A. U., Blanchard, C. and Naqvi, S. M. S. (2018) A low molecular weight serine protease inhibitor isolated from Nigella sativa seeds shows homology to non-specific lipid transfer proteins. (submited)

Subject Matter Expertise

• ★ Biochemistry
• ☆ Virology
• ☆ Molecular Biology
• ☆ Proteomics
• ☆ Cell Signaling Pathways
• ☆ Basic Immunology
• ☆ Biotechnology & Bioengineering

Services

• Writing
• Research

Writing Technical Writing

Research Gray Literature Search

Work Experience

Assistant Professor of Biochemistry

PMAS UAAR

October 2018 - Present

Education

PhD, Biochemistry

PMAS Arid Agriculture University Rawalpindi

September 2009 - November 2016

Certifications

• M Phil

  PMAS Arid Agriculture University Rawalpindi

  September 2007 - September 2009