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Hire Dr. Muhammad Nouman M.

Canada

USD 40 /hr

PhD Biomedical Scientist | Oncology Biomarkers, Transcriptomics & Molecular Diagnostics

Profile Summary

I am a PhD Cell & Molecular Biologist with extensive experience in glioblastoma, cancer biomarkers, gene expression profiling, and translational research. My work focuses on miRNA/mRNA analysis, qPCR assay development, RNA-seq data interpretation, molecular diagnostics, and biomarker validation. I have led multiple clinical and laboratory studies and authored 30+ peer-reviewed publications in neuro-oncology, autophagy, epigenetics, and cancer biology. I help biotech and diagnostic teams with: • Biomarker discovery and validation (miRNA, mRNA, DNA markers) • qPCR assay optimization and data analysis • RNA-seq, differential expression, and pathway analysis • Study design for diagnostic and translational projects • Clinical sample analysis (tissue, serum, FFPE) • Protocol development and troubleshooting • Scientific writing (manuscripts, reports, methods, grant documents)

Subject Matter Expertise

• ☆ Data Analysis
• ☆ Social Network Analysis
• ☆ Drug Discovery
• ☆ High Throughput Screening (HTS)
• ☆ Drug Target Identification
• ☆ High Content Screening/Imaging
• ☆ Vaccine Development
• ☆ In vitro Diagnostics
• ☆ In vitro Diagnostic Validation
• ☆ In vitro Diagnostic Design
• ☆ Biomarker Analysis
• ☆ Clinical Trials
• ☆ IRB Applications
• ☆ Clinical Data Management
• ☆ Pharmaceutical Technology
• ☆ Product Validation
• ☆ Concept Development
• ☆ Hypothesis
• ☆ Peer Review
• ☆ Research Methodology
• ☆ Survey Design & Methodology
• ☆ Meta-Research
• ☆ Feasibility Studies
• ☆ Literature Search
• ☆ Gap Analysis
• ☆ Process Design
• ☆ Continuing Medical Education (CME) Training
• ☆ Skills Training
• ☆ Medical Writing
• ☆ Research Summaries & Abstracts
• ☆ Technical & Scientific Writing
• ☆ Journal Selection
• ☆ Manuscript & Journal Article Writing
• ☆ Instructional Materials
• ☆ Experimental Protocols
• ☆ SOP & Protocol Writing
• ☆ Science Communication
• ☆ Literature Review
• ☆ Traditional/Narrative Literature Review
• ☆ Systematic Literature Review
• ☆ Scientific Proofreading & Editing
• ☆ Proofreading & Editing
• ☆ Course Content Writing
• ☆ Digital Marketing

Services

• Writing
• Research
• Data & AI

Writing Non-Medical Regulatory Writing, Technical Writing, Copywriting

Research Meta-Research

Data & AI Image Processing, Image Analysis, Algorithm Design-Non ML

Work Experience

Assistant Professor

Aga Khan University

February 2019 - Present

Assistance Professor

DOW University of Health Sciences (DUHS)

October 2013 - February 2019

Education

PhD

Karolinska Institute Stockholm - Sweden

October 2009 - June 2013

MSC (Biotechnology )

University of Karachi

January 2005 - December 2006

BSC (Biochemistry, Chemistry and Microbiology )

University of Karachi

January 2002 - 2005

Certifications

• NA

  n/a

  January 2025 - January 2025

Publications

JOURNAL ARTICLE

Sufiyan Sufiyan, Hira Salam, Sahar Ilyas, Wajiha Amin, Fatima Arshad, Kainaat Fatima, Sana Naeem, Altaf Ali Laghari, Syed Ather Enam, Nouman Mughal (2025). Prognostic implications of DNA methylation machinery (DNMTs and TETs) expression in gliomas: correlations with tumor grading and patient survival . Journal of Neuro-Oncology.

Muhammad Nouman Mughal, Nida Zahid, Nick Brown, Andreas Mårtensson, Thomas Mårtensson, Muhammad Sufiyan Ibrahim, Sana Naeem, Siraj Qazi, Wajiha Amin, Iqbal Azam, et al. (2025). MicroRNA Expression and Neurocognitive Outcomes in Children and Young People With Primary Brain Tumor in Karachi, Pakistan: A Pilot Exploratory Study . Brain Tumor Research and Treatment.

Muhammad Nouman Mughal, Nida Zahid, Syed Ather Enam, Thomas Mårtensson, Iqbal Azam, Naureen Mushtaq, Mariya Moochhala, Aneesa Hassan, Faiza Kausar, Saqib Bakhshi, et al. (2024). Sociodemographic and clinical predictors of quality-of-life outcome in children and young people with primary brain tumour in Karachi, Pakistan: a prospective cohort study . BMJ Paediatrics Open.

Tuba Ghazal, Muhammad Adeel Ahmed, Fazal-ur-Rehman Qazi, Muhammad Mohsin Haider, Sana Naeem, Rizwan Jouhar, Muhammad Farooq Umer, Muhammad Faheemuddin, Veeriah Chowdary Jasthi, Nouman Mughal (2024). Mutational analysis of cytoplasmic domain of integrin subunit alpha-1 and its association with periapical wound healing after surgical endodontic treatment . PLOS ONE.

Wajiha Amin, Peyman Tabnak, Syed Ather Enam, Sufiyan Sufiyan, Kulsoom Ghias, Mohammad Hamza Bajwa, Sahar Ilyas, Altaf Ali Laghari, Sana Naeem, Syed Hani Abidi, et al. (2024). Autophagy-associated biomarkers ULK2, UVRAG, and miRNAs miR-21, miR-126, and miR-374: Prognostic significance in glioma patients . PLOS ONE.

Wajiha Amin, Syed Ather Enam, Sufiyan Sufiyan, Kulsoom Ghias, Mohammad Hamza Bajwa, Sahar Ilyas, Altaf Ali Laghari, Sana Naeem, Syed Hani Abidi, Nouman Mughal (2024). Autophagy-associated biomarkers ULK2, UVRAG, and miRNAs miR-21, miR-126, and miR-374: Prognostic significance in glioma patients . PLOS ONE.

Mohammad Hamza Bajwa, Muhammad Awais Khan, Faiza Urooj, Muhammad Usman Khalid, Syed Ather Enam, Hafiza Fatima Aziz, Kaynat Siddiqui, Muhammad Ehsan Bari, Nouman Mughal (2024). Serum liquid biopsy for brain tumours: a scoping analysis of practicable approaches in low- and middle-income countries . Journal of the Pakistan Medical Association.

Mohammad Hamza Bajwa, Muhammad Awais Khan, Faiza Urooj, Muhammad Usman Khalid, Syed Ather Enam, Hafiza Fatima Aziz, Kaynat Siddiqui, Muhammad Ehsan Bari, Nouman Mughal (2024). Serum liquid biopsy for brain tumours: a scoping analysis of practicable approaches in low- and middle-income countries . Journal of the Pakistan Medical Association.

Muhammad Nouman Mughal, Nida Zahid, Syed Ather Enam, Thomas Mårtensson, Iqbal Azam, Naureen Mushtaq, Mariya Moochhala, Faiza Kausar, Aneesa Hassan, Saqib Kamran Bakhshi, et al. (2024). Factors associated with changes in the quality of life and family functioning scores of primary caregivers of children and young people with primary brain tumors in Karachi, Pakistan: a prospective cohort study . BMC Pediatrics.

Nida Zahid, S. Ather Enam, Thomas Mårtensson, Iqbal Azam, Naureen Mushtaq, Mariya Moochhala, Farrukh Javed, Faiza Kausar, Aneesa Hasan, Lal Rehman, et al. (2024). Predictors of neurocognition outcomes in children and young people with primary brain tumor presenting to tertiary care hospitals of Karachi, Pakistan: a prospective cohort study . Child's Nervous System.

Nida Zahid, S. Ather Enam, Thomas Mårtensson, Iqbal Azam, Naureen Mushtaq, Mariya Moochhala, Farrukh Javed, Faiza Kausar, Aneesa Hasan, Lal Rehman, et al. (2024). Predictors of neurocognition outcomes in children and young people with primary brain tumor presenting to tertiary care hospitals of Karachi, Pakistan: a prospective cohort study . Child's Nervous System.

Mushtaq N, Enam SA, Bajwa MH, Mustansir F, Baig E, Gauhar F, Arif A, Khan AA, Hamid SA, Javed G, et al.(2024). Consensus guidelines for the management of pediatric medulloblastoma in low and middle-income countries . JPMA. The Journal of the Pakistan Medical Association.

Farheen Danish, Muhammad Asif Qureshi, Talat Mirza, Wajiha Amin, Sufiyan Sufiyan, Sana Naeem, Fatima Arshad, Nouman Mughal (2024). Investigating the Association between the Autophagy Markers LC3B, SQSTM1/p62, and DRAM and Autophagy-Related Genes in Glioma . International Journal of Molecular Sciences.

Yuling Meng, Nouman Mughal, Indrani Datta, Oluwademilade Nuga, Susan Irtenkauf, Laura Hasselbach, Kristyn Quenneville, Stephen Brown, Ana deCarvalho (2023). EXTH-06. GLIOBLASTOMA PATIENT SPECIFIC TRANSCRIPTOME REMODELING IN RESPONSE TO MDM2 ANTAGONIST TREATMENT OF WILD-TYPE P53 CANCER STEM CELLS IS ASSOCIATED WITH SENSITIVITY AND STRATEGIES FOR COMBINATION THERAPY . Neuro-Oncology.

Hira Salam, Shaheen Ahmed, Muhammad Furqan Bari, Uzma Bukhari, Ghulam Haider, Shariq Najeeb, Nouman Mughal (2023). Association of Kaiso and partner proteins in oral squamous cell carcinoma . Journal of Taibah University Medical Sciences.

Muhammad Nouman Mughal, Nida Zahid, Syed Ather Enam, Faiza Urooj, Russell Seth Martins, Thomas Mårtensson, Andreas Mårtensson, Naureen Mushtaq, Faiza Kausar, Mariya Moochhala, et al. (2023). Socio-demographic and antenatal risk factors of brain tumor in children and young people: A matched case-control study from Karachi, Pakistan . Journal of Public Health Research.

Unzela Ghulam, Fizza Nazim, Nida Farooqui, Syed Rizwan-ul-Hasan, Muhammad Faraz Anwar, Khalid Ahmed, Abid Jamal, Hammad Afzal Kayani, Nouman Mughal, Azhar Hussain, et al. (2022). Analysis of differential gene expression of pro-inflammatory cytokines in the nasopharyngeal milieu of mild & severe COVID-19 cases . PLOS ONE.

Muhammad Nouman Mughal, Shaheen Ahmed, Saeed Khan, Muhammad Asif Qureshi, Uzma Bukhari, Mehak Anis (2022). Expressional variations of Kaiso: an association with pathological characteristics and field cancerization of OSCC . BMC Cancer.

Muhammad Adeel Ahmed, Fizza Nazim, Khalid Ahmed, Muhammad Furqan Bari, Abdulaziz Abdulwahed, Ahmed A. AlMokhatieb, Yaseen Alalvi, Tariq Abduljabbar, Nouman Mughal, Syed Hani Abidi (2022). Association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment . Scientific Reports.

Muhammad Adeel Ahmed, Fizza Nazim, Khalid Ahmed, Muhammad Furqan Bari, Abdulaziz Abdulwahed, Ahmed A. AlMokhatieb, Yaseen Alalvi, Tariq Abduljabbar, Nouman Mughal, Syed Hani Abidi (2022). Association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment . Scientific Reports.

Khalid Ahmed, Alisalman Sheikh, Saira Fatima, Ghulam Haider, Kulsoom Ghias, Farhat Abbas, Nouman Mughal, Syed Hani Abidi (2022). Detection and characterization of latency stage of EBV and histopathological analysis of prostatic adenocarcinoma tissues . Scientific Reports.

Mughal, Muhammad Nouman, Ahmed, Shaheen, Khan, Saeed, Qureshi, Muhammad Asif, Bukhari, Uzma, Anis, Mehak (2022). Expressional variations of Kaiso: an association with pathological characteristics and field cancerization of OSCC . BMC Cancer.

Muhammad Adeel Ahmed, Nouman Mughal, Syed Hani Abidi, Muhammad Furqan Bari, Mohammed Mustafa, Fahim Vohra, Ali Alrahlah (2021). Factors Affecting the Outcome of Periapical Surgery; a Prospective Longitudinal Clinical Study . Applied Sciences.

Muhammad Adeel Ahmed, Muhammad Faraz Anwar, Khalid Ahmed, Marziya Aftab, Fizza Nazim, Muhammad Furqan Bari, Mohammed Mustafa, Fahim Vohra, Ali Alrahlah, Nouman Mughal, et al. (2021). Baseline MMP expression in periapical granuloma and its relationship with periapical wound healing after surgical endodontic treatment . BMC Oral Health.

Farheen Danish, Hira Salam, Muhammad Asif Qureshi, Mughal Nouman (2021). Comparative clinical and epidemiological study of central nervous system tumors in Pakistan and global database . Interdisciplinary Neurosurgery.

Mehwish Sajjad, Syed Ali, Samina Baig, Shaheen Sharafat, Bilal Ahmed Khan, Saeed Khan, Nouman Mughal, Syed Hani Abidi (2021). HBV S antigen evolution in the backdrop of HDV infection affects epitope processing and presentation . Journal of Medical Virology.

Mughal, Muhammad Nouman, Sajjad, Mehwish, Ali, Syed, Baig, Samina, Sharafat, Shaheen, Khan, Bilal Ahmed, Khan, Saeed, Abidi, Syed Hani (2021). HBV S antigen evolution in the backdrop of HDV infection affects epitope processing and presentation . Journal of Medical Virology.

Saeed Khan, Maria Zahid, Muhammad Asif Qureshi, Nouman Mughal, Ikram Din Ujjan (2018). Correction to: HIV-1 genetic diversity, geographical linkages and antiretroviral drug resistance among individuals from Pakistan . Archives of Virology.

Mughal, Muhammad Nouman, Khan, Saeed, Zahid, Maria, Qureshi, Muhammad Asif, Ujjan, Ikram Din (2018). HIV-1 genetic diversity, geographical linkages and antiretroviral drug resistance among individuals from Pakistan . Archives of Virology.

N Azhar, M Sohail, F Ahmad, S Fareeha, S Jamil, N Mughal, H Salam (2018). Risk factors of Oral cancer- A hospital based case control study . Journal of Clinical and Experimental Dentistry.

Syed H. Abidi, Khalid Ahmed, Fatin Zehra, Nouman Mughal, Shahana U. Kazmi (2016). Rabbit Serum Raised against P. aeruginosa Biofilm Exhibit Significant Biofilm Reduction and Removal Activity . International Journal of Current Microbiology and Applied Sciences.

Mughal, Muhammad Nouman, Noori, Muhammad Yahya, Ali, Zaheer, Wahidi, Syed Adnan Ali, Sharafat, Shaheen, Masroor, Muhammad, Khanani, Muhammad Rafiq (2016). False negativity in AFB Smear microscopy: An insight into the caveats of the most widely used screening tool for tuberculosis . The Journal of the Pakistan Medical Association.

Nouman Mughal (2016). False negativity in AFB Smear microscopy: An insight into the caveats of the mostwidely usedscreening tool for tuberculosis .

Nouman Mughal, Giuseppe Coppotelli, Simone Callegari, Stefano Gastaldello, Maria G. Masucci (2013). Interaction of Gamma-Herpesvirus Genome Maintenance Proteins with Cellular Chromatin . PLoS ONE.

Giuseppe Coppotelli, Nouman Mughal, Simone Callegari, Ramakrishna Sompallae, Laia Caja, Martijn S. Luijsterburg, Nico P. Dantuma, Aristidis Moustakas, Maria G. Masucci (2013). The Epstein–Barr virus nuclear antigen-1 reprograms transcription by mimicry of high mobility group A proteins . Nucleic Acids Research.

Giuseppe Coppotelli, Nouman Mughal, Maria G. Masucci (2013). The Gly–Ala repeat modulates the interaction of Epstein–Barr virus nuclear antigen-1 with cellular chromatin . Biochemical and Biophysical Research Communications.

Giuseppe Coppotelli, Nouman Mughal, Diego Marescotti, Maria G. Masucci (2011). High Avidity Binding to DNA Protects Ubiquitylated Substrates from Proteasomal Degradation . Journal of Biological Chemistry.

Nadeem-ul-Haq, Arain MA, Haque Z, Badar N, Mughal N(2009). Drinking water contamination by chromium and lead in industrial lands of Karachi . JPMA. The Journal of the Pakistan Medical Association.

Nouman Mughal, Nouman Mughal, Nouman Mughal, Nouman Mughal, Nouman Mughal, Nouman Mughal, Nouman Mughal Journal of Liaquat University of Medical & Health Sciences .

PREPRINT

Khalid Ahmed, Mahmoud A. A. Ibrahim, Farhat Abbas, Peter A. Sidhom, Mohamed-Elamir F. Hegazy, Nouman Mughal, Shaban R. M. Sayed, Kulsoom Ghias, Syed Hani Abidi (2023). In Silico Characterization of Putative Ebv Receptor(S) on Prostate Cells and Repurposing Clinically Approved Drugs as Ebv Entry Inhibitors .

Ahmed K, Sheikh A, Fatima S, Haider G, Abbas F, Ghias K, Mughal N, Abidi SH(2022). Assessment of histopathological parameters and survival difference in EBV-positive and EBV-negative prostate carcinoma .

REVIEW

Nouman Mughal (2020). Salivary Biomarkers for Oral Squamous Cell Carcinoma: a meta-analysis. Pak J Microbio.