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Hire Dr. Michael James H.

Portugal

USD 60 /hr

Bioimaging Expert, Specialist in Electron Microscopy

Profile Summary

I have a background in Biomedical Sciences, completing my BSc degree (Hons) in 2016. My PhD combined molecular and biochemical techniques with fluorescence and electron microscopy (EM), complementing and enriching studies using conventional EM and specialised techniques such as immuno-TEM, CLEM and 3D TEM. I continued to specialise in application of innovative imaging solutions in my postdoctoral studies, developing novel approaches to decipher complex questions by using EM. I now operate and manage the new EM Service at NMS, providing consultancy, full EM service, data interpretation and manuscript preparation assistance to researchers. I am able to assess project feasibility based on preliminary experiments, advise suggested preliminary experiments, select appropriate imaging (EM) techniques for the scientific question and specimen type, and design tailored EM protocols.

Subject Matter Expertise

• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cell Signaling Pathways
• ☆ Membrane Biology
• ☆ Stem Cell Research
• ☆ Microscopy
• ☆ Cryosectioning
• ☆ Light Microscopy
• ☆ Electron Microscopy
• ☆ Fluorescence Microscopy
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Cell Surface Receptors
• ☆ 3D Cell Culture
• ☆ Cell Aging
• ☆ Medical Sciences

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing General Proofreading & Editing

Research Feasibility Study, Technology Scouting, Fact Checking, Gap Analysis, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Image Processing, Image Analysis, Data Visualization

Product Development Concept Development

Work Experience

Bioimaging Technician (Electron Microscopy Specialist)

Universidade Nova de Lisboa Faculdade de Ciências Médicas

July 2022 - Present

Bioimaging Senior Technician (Electron Microscopy)

NOVA Medical School, Universiade NOVA de Lisboa

January 2022 - Present

Postdoctoral Researcher

Universidade Nova de Lisboa Centro de Estudos de Doenças Crónicas

October 2020 - March 2022

Research Technician

UCL Institute of Ophthalmology, University College London

December 2019 - September 2020

Education

Doctor of Philosophy (Ph.D)

University College London, University of London - United Kingdom

September 2016 - October 2020

BSc Biomedical Sciences (School of Biological and Chemical Sciences/School of Medicine and Dentistry)

Queen Mary University of London

August 2013 - August 2016

Certifications

• Certification details not provided.

Publications

WORKING PAPER

Photoprotective melanin is maintained within keratinocytes in a storage lysosome @unpublished { author = {Hall, Michael J}, title = {Photoprotective melanin is maintained within keratinocytes in a storage lysosome}, year = {2024}, month = {feb}, url = {https://www.biorxiv.org/content/10.1101/2024.02.05.578910v1} } .

Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer @unpublished { author = {Hall, Michael J}, title = {Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer}, year = {2022}, month = {mar}, url = {https://www.biorxiv.org/content/10.1101/2022.03.03.482849v1}, doi = {https://doi.org/10.1101/2022.03.03.482849; 10.1111/pcmr.13039} } .

JOURNAL ARTICLE

Deacidification of endolysosomes by neuronal aging drives synapse loss @article { author = {Burrinha, T. and Cunha, C. and Hall, M.J. and Lopes-da-Silva, M. and Seabra, M.C. and Guimas Almeida, C.}, title = {Deacidification of endolysosomes by neuronal aging drives synapse loss}, journal = {Traffic}, volume = {24}, number = {8}, pages = {334--354}, year = {2023}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-85159868625&partnerID=MN8TOARS}, doi = {10.1111/tra.12889} } . Traffic.

Burrinha, T., Cunha, C., Hall, M.J., Lopes-da-Silva, M., Seabra, M.C., Guimas Almeida, C.(2023). Deacidification of endolysosomes by neuronal aging drives synapse loss . Traffic. 24. (8). p. 334-354.

Cardoso, M.H., Hall, M.J., Burgoyne, T., Fale, P., Storm, T., Escrevente, C., Antas, P., Seabra, M.C., Futter, C.E.(2023). Impaired Lysosome Reformation in Chloroquine-Treated Retinal Pigment Epithelial Cells . Investigative Ophthalmology and Visual Science. 64. (11).

Impaired Lysosome Reformation in Chloroquine-Treated Retinal Pigment Epithelial Cells @article { author = {Cardoso, M.H. and Hall, M.J. and Burgoyne, T. and Fale, P. and Storm, T. and Escrevente, C. and Antas, P. and Seabra, M.C. and Futter, C.E.}, title = {Impaired Lysosome Reformation in Chloroquine-Treated Retinal Pigment Epithelial Cells}, journal = {Investigative Ophthalmology and Visual Science}, volume = {64}, number = {11}, year = {2023}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-85166771039&partnerID=MN8TOARS}, doi = {10.1167/iovs.64.11.10} } . Investigative Ophthalmology and Visual Science.

Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease-activated receptor-2 internalization @article { author = {Moreiras, Hugo and Bento-Lopes, Liliana and Neto, Matilde V. and Escrevente, Cristina and Cabaço, Luís C. and Hall, Michael J. and Ramalho, José S. and Seabra, Miguel C. and Barral, Duarte C.}, title = {Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease-activated receptor-2 internalization}, journal = {Traffic}, volume = {23}, number = {6}, pages = {331--345}, year = {2022}, month = {may}, url = {http://dx.doi.org/10.1111/tra.12843}, doi = {10.1111/tra.12843} } . Traffic.

Current methods to analyse lysosome morphology, positioning, motility and function. @article { title = {Current methods to analyse lysosome morphology, positioning, motility and function.}, journal = {Traffic (Copenhagen, Denmark)}, year = {2022}, month = {mar}, url = {https://doi.org/10.1111/tra.12839}, doi = {10.1111/tra.12839} } . Traffic (Copenhagen, Denmark).

(2022). Current methods to analyse lysosome morphology, positioning, motility and function . Traffic (Copenhagen, Denmark).

(2022). Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer . Pigment cell & melanoma research.

Barral, D.C., Staiano, L., Guimas Almeida, C., Cutler, D.F., Eden, E.R., Futter, C.E., Galione, A., Marques, A.R.A., Medina, D.L., Napolitano, G., et al.(2022). Current methods to analyze lysosome morphology, positioning, motility and function . Traffic. 23. (5). p. 238-269.

Hall, M.J., Lopes-Ventura, S., Neto, M.V., Charneca, J., Zoio, P., Seabra, M.C., Oliva, A., Barral, D.C.(2022). Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer . Pigment Cell and Melanoma Research. 35. (4). p. 425-435.

Moreiras, H., Bento-Lopes, L., Neto, M.V., Escrevente, C., Cabaço, L.C., Hall, M.J., Ramalho, J.S., Seabra, M.C., Barral, D.C.(2022). Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease-activated receptor-2 internalization . Traffic. 23. (6). p. 331-345.

Cristina Escrevente and Ana S. Falc{\~{a}}o and Michael J. Hall and Mafalda Lopes-da-Silva and Pedro Antas and Miguel M. Mesquita and In{\^{e}}s S. Ferreira and M. Helena Cardoso and Daniela Oliveira and Ana C. Fradinho and Thomas Ciossek and Paul Nicklin and Clare E. Futter and Sandra Tenreiro and Miguel C. Seabra(2021). Formation of Lipofuscin-Like Autofluorescent Granules in the Retinal Pigment Epithelium Requires Lysosome Dysfunction . Investigative Opthalmology & Visual Science. 62. (9). p. 39. Association for Research in Vision and Ophthalmology ({ARVO})

Formation of Lipofuscin-Like Autofluorescent Granules in the Retinal Pigment Epithelium Requires Lysosome Dysfunction @article { author = {Hall, Michael J}, title = {Formation of Lipofuscin-Like Autofluorescent Granules in the Retinal Pigment Epithelium Requires Lysosome Dysfunction}, journal = {Investigative Opthalmology & Visual Science}, volume = {62}, number = {9}, pages = {39--39}, year = {2021}, month = {jul}, url = {http://dx.doi.org/10.1167/iovs.62.9.39}, doi = {10.1167/iovs.62.9.39} } . Investigative Opthalmology & Visual Science.

Escrevente, C., Falcão, A.S., Hall, M.J., Lopes-Da-Silva, M., Antas, P., Mesquita, M.M., Ferreira, I.S., Helena Cardoso, M., Oliveira, D., Fradinho, A.C., et al.(2021). Formation of lipofuscin-like autofluorescent granules in the retinal pigment epithelium requires lysosome dysfunction . Investigative Ophthalmology and Visual Science. 62. (9).

Is Endovascular Treatment Still Good for Ischemic Stroke in Real World?: A Meta-Analysis of Randomized Control Trial and Observational Study in the Last Decade. @article { author = {Hall, Michael}, title = {Is Endovascular Treatment Still Good for Ischemic Stroke in Real World?: A Meta-Analysis of Randomized Control Trial and Observational Study in the Last Decade.}, journal = {Stroke}, year = {2020}, month = {sep}, url = {https://doi.org/10.1161/STROKEAHA.120.029742}, doi = {10.1161/strokeaha.120.029742} } . Stroke.

(2020). Is Endovascular Treatment Still Good for Ischemic Stroke in Real World?: A Meta-Analysis of Randomized Control Trial and Observational Study in the Last Decade . Stroke.

Zhao, Z., Zhang, J., Jiang, X., Wang, L., Yin, Z., Hall, M., Wang, Y., Lai, L.(2020). Is Endovascular Treatment Still Good for Ischemic Stroke in Real World?: A Meta-Analysis of Randomized Control Trial and Observational Study in the Last Decade . Stroke. 51. (11). p. 3250-3263.

OTHER

Hall, M.J., Lopes-Ventura, S., Neto, M.V., Charneca, J., Zoio, P., Seabra, M.C., Oliva, A., Barral, D.C.(2022). Reconstructed human pigmented skin/epidermis models achieve epidermal pigmentation through melanocore transfer . bioRxiv.