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Hire Dr. Mark S.

United States

Profile Summary

I have extensive experience experimental design, troubleshooting, and analysis in a variety of biological fields. I have authored and edited numerous academic publications and grants. I have extensive experience in reviewing scientific literature, and extracting the salient points for my research. In general, I am interested in the relationship between a protein's structure and its biological function. The goal of my current research is to determine a high resolution structure of the yeast spindle pole body (SPB). The SPB is the microtubule organizing center in budding yeast, and it consists of a multi-protein array that spans the yeast nucleus. This work involves x-ray crystallography, electron microscopy, various biophysical methods, and protein expression and purification in bacteria and insect cells. As a postdoctoral fellow, I investigated the role of the ganglioside GM3 in inhibiting wound healing under diabetic conditions. I also developed and tested a novel siRNA-conjugated nanoparticle that was able to reverse this wound healing defect. This work involved mammalian cell culture, mouse surgery and dissection, qPCR, preparation and analysis of histological samples, and various microscopy techniques. As a PhD student, I investigated the structures of the kinesin molecular motor tail domains and how these structures facilitate ligand binding. This work involved electron microscopy, CD spectroscopy, SAXS, x-ray crystallography, NMR, and ITC. During and after my undergraduate studies I worked in various laboratories as a student or a research technician, and learned a variety of molecular and cell biology techniques.

Subject Matter Expertise

• ☆ Separation & Purification
• ☆ X-Ray Crystallography
• ☆ NMR Spectroscopy
• ☆ Biochemistry
• ☆ Molecular Biology
• ☆ Biophysics
• ☆ Microscopy
• ☆ Data Analysis
• ☆ Cell Culture
• ☆ Microbial Fermentation
• ☆ Protein/Enzyme Engineering
• ☆ Research
• ☆ Research Methodology
• ☆ Technical & Scientific Writing

Services

• Writing
• Consulting
• Data & AI
• Product Development

Work Experience

Assistant Scientist

University of Wisconsin Madison

2014 - Present

Postdoctoral Fellow

Northwestern University

2012 - 2014

Research Technician

Northwestern University

2004 - 2006

Research Technician

University of Chicago

2002 - 2004

Student Researcher

University of Chicago

1999 - 2002

Student Researcher

University of Chicago

1999 - 2002

Education

PhD Biophysics (Cell and Molecular Biology)

Northwestern University

2006 - 2012

BA/BS Chemistry/Biochemistry

University of Chicago

1998 - 2002

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2015). siRNA-Based Spherical Nucleic Acids Reverse Impaired Wound Healing in Diabetic Mice by Ganglioside GM3 Synthase Knockdown. Proc Natl Acad Sci.

(2015). The Roles of Growth Factors in Keratinocyte Migration. Adv Wound Care.

(2014). Ganglioside GM3 Depletion Reverses Impaired Wound Healing in Diabetic Mice by Activating IGF-1 and Insulin Receptors. J Invest Dermatol.

(2014). The Role of Abnormalities in the Distal Pathway of Cholesterol Synthesis in the Congenital Hemidysplasia with Ichthyosiform Erythroderma and Limb Defects (CHILD) Syndrome. Biochim Biophys Acta.

(2013). Intrinsic Disorder in the Kinesin Superfamily. Biophys Rev.

(2012). Kinesin Tail Domains Are Intrinsically Disordered. Proteins.

(2010). Microtubule-associated Protein-like Binding of the Kinesin-1 Tail to Microtubules. J Biol Chem.

(2008). SV40 Reporter Viruses. J Virol Methods.

(2004). NOVOcan: A Molecular Link Among Selected Glial Cells. Biophys Chem.

(2003). Oligodendrocyte Phenotypical and Morphological Heterogeneity: A Reexamination of Old Concepts in View of New Findings. Adv Mol Cell Biol.