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Hire Dr. Maria S.

Greece

USD 200 /hr

Microbiologist, Molecular biologist / Expert in microbial biofilms/ Biology content writer with 10+ years of experience

Profile Summary

A. selected previous and ongoing research projects: 1) Mutational analysis of a yeast mitochondrial mRNA binding protein. 2) Ribosomal protein gene disruption in Thermus thermophilus. 3) Comparative Effects of antimicrobials on cytokine expression profiles in human monocytes. 4) Mechanisms of the Immunomodulatory Effects of antimicrobial agents on Human Monocytes exposed to fungi: Regulation of Pathogen Recognition, Signal Transduction and Cytokine Expression Profiles. 5) Immunomodulatory effects of antifungals on host responses against microbial biofilms. B. Research interests: 1Antimicrobial drug susceptibility, Microbial-Host interactions, Microbial biofilms and the Host, Drug immunomodulatory effects C. Peer reviewing: invited reviewer for several international scientific journals.

Subject Matter Expertise

• ★ Microbiology
• ★ Molecular Biology
• ☆ Mycology/Fungi
• ☆ Mutagenesis
• ☆ Cell Signaling Pathways
• ☆ Microbial Fermentation
• ☆ Peer Review

Services

• Writing
• Research
• Consulting
• Product Development

Work Experience

Senior investigator

Laboratory of Infectious Diseases, 3rd Department of Pediatrics, Hippokration Hospital, School of Medicine, Aristotle University of Thessaloniki, Greece

January 2001 - Present

Associate researcher

Dept. of Biochemistry, School of Chemistry, Aristotle University of Thessaloniki, Greece.

January 1998 - January 2000

Post doctoral position

Max-Planck Institute of Molecular Genetics, Berlin, Germany

January 1997 - January 1999

Associate researcher

Laboratory of Food Chemistry and Biochemistry, Dept. of Dairy Technology, Aristotle University of Thessaloniki, Greece.

January 1996 - January 1997

Associate researcher

Laboratory of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Greece.

January 1994 - January 1995

Post-doctoral position

Dept of Molecular Biology , University of Amsterdam, Holland

January 1991 - January 1992

Education

MSc, PhD

Bowling Green State University, Ohio, USA

January 1988 - January 1991

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2017). Performance of QuantiFERON®-TB Gold In-Tube assay in children receiving disease modifying anti-rheumatic drugs . World Journal of Pediatrics. 13. (5). p. 472-478.

Roilides, E., Simitsopoulou, M.(2017). Immune responses to Mucorales growth forms: Do we know everything? . Virulence. 8. (8). p. 1489-1491.

(2015). How biofilms evade host defenses . Microbiology Spectrum. 3. (3).

(2015). Methylprednisolone impairs conidial phagocytosis but does not attenuate hyphal damage by neutrophils against Exserohilum rostratum . Medical Mycology. 53. (2). p. 189-193.

(2015). Antipseudomonal agents exhibit differential pharmacodynamic interactions with human polymorphonuclear leukocytes against established biofilms of Pseudomonas aeruginosa . Antimicrobial Agents and Chemotherapy. 59. (4). p. 2198-2205.

Tratselas, A., Simitsopoulou, M., Giannakopoulou, A., Dori, I., Saoulidis, S., Kollios, K., Papaioannidou, P., Pournaras, S., Roilides, E.(2014). Effect of ceftriaxone on the outcome of murine pyelonephritis caused by extended-spectrum-β-lactamase-producing Escherichia coli . Antimicrobial Agents and Chemotherapy. 58. (12). p. 7102-7111.

Simitsopoulou, M., Kyrpitzi, D., Velegraki, A., Simitsopoulou, M., Kyrpitzi, D., Velegraki, A., Walsh, T.J., Roilides, E.(2014). Caspofungin at catheter lock concentrations eradicates mature biofilms of Candida lusitaniae and Candida guilliermondii . Antimicrobial Agents and Chemotherapy. 58. (8). p. 4953-4956.

(2013). Species-specific and drug-specific differences in susceptibility of candida biofilms to echinocandins: Characterization of less common bloodstream isolates . Antimicrobial Agents and Chemotherapy. 57. (6). p. 2562-2570.

Katragkou, A., Chatzimoschou, A., Simitsopoulou, M., Georgiadou, E., Roilides, E.(2011). Additive antifungal activity of anidulafungin and human neutrophils against Candida parapsilosis biofilms . Journal of Antimicrobial Chemotherapy. 66. (3). p. 588-591.

(2011). Differential transcriptional profiles induced by amphotericin B formulations on human monocytes during response to hyphae of Aspergillus fumigatus . Medical Mycology. 49. (2). p. 176-185.

Chatzimoschou, A., Katragkou, A., Simitsopoulou, M., Antachopoulos, C., Georgiadou, E., Walsh, T.J., Roilides, E.(2011). Activities of triazole-echinocandin combinations against Candida species in biofilms and as planktonic cells . Antimicrobial Agents and Chemotherapy. 55. (5). p. 1968-1974.

Simitsopoulou, M., Roilides, E., Walsh, T.J.(2011). Immunomodulatory properties of antifungal agents on phagocytic cells . Immunological Investigations. 40. (7-8). p. 809-824.

(2011). Ventriculitis caused by Aspergillus fumigatus in a child with central nervous system tuberculosis . Mycoses. 54. (5).

(2011). Effects of interferon-γ and granulocyte colony-stimulating factor on antifungal activity of human polymorphonuclear neutrophils against Candida albicans grown as biofilms or planktonic cells . Cytokine. 55. (3). p. 330-334.

Simitsopoulou, M., Georgiadou, E., Walsh, T.J., Roilides, E.(2010). Cunninghamella bertholletiae exhibits increased resistance to human neutrophils with or without antifungal agents as compared to Rhizopus spp . Medical Mycology. 48. (5). p. 720-724.

Roilides, E., Simitsopoulou, M.(2010). Local innate host response and filamentous fungi in patients with cystic fibrosis . Medical Mycology. 48. (O1).

(2010). Interactions between human phagocytes and candida albicans biofilms alone and in combination with antifungal agents . Journal of Infectious Diseases. 201. (12). p. 1941-1949.

(2008). Differential activities of newer antifungal agents against Candida albicans and Candida parapsilosis biofilms . Antimicrobial Agents and Chemotherapy. 52. (1). p. 357-360.

(2008). Interaction of amphotericin B lipid formulations and triazoles with human polymorphonuclear leucocytes for antifungal activity against Zygomycetes . Mycoses. 51. (2). p. 147-154.

Simitsopoulou, M., Roilides, E., Likartsis, C., Ioannidis, J., Orfanou, A., Paliogianni, F., Walsh, T.J.(2007). Expression of immunomodulatory genes in human monocytes induced by voriconazole in the presence of Aspergillus fumigatus . Antimicrobial Agents and Chemotherapy. 51. (3). p. 1048-1054.

(2006). Generation of highly purified and functionally active human TH1 cells against Aspergillus fumigatus . Blood. 107. (6). p. 2562-2569.

(2005). Inteferon gamma and granulocyte-macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes against Scedosporium spp.: Comparison with Aspergillus spp . Medical Mycology. 43. (3). p. 253-260.

(2005). Evaluation of immunotherapy in invasive candidiasis: antifungal activity and cytokine expression assays . Methods in molecular medicine. 118. p. 161-179.

(2005). Differential expression of cytokines and chemokines in human monocytes induced by lipid formulations of amphotericin B . Antimicrobial Agents and Chemotherapy. 49. (4). p. 1397-1403.

Winn, R.M., Gil-Lamaignere, C., Roilides, E., Simitsopoulou, M., Winn, R.M., Gil-Lamaignere, C., Roilides, E., Simitsopoulou, M., Lyman, C.A., Maloukou, A., et al.(2005). Effects of interleukin-15 on antifungal responses of human polymorphonuclear leukocytes against Fusarium spp. and Scedosporium spp . Cytokine. 31. (1). p. 1-8.

(2005). Interferon-γ and granulocyte-macrophage colony-stimulating factor augment the activity of polymorphonuclear leukocytes against medically important zygomycetes . Journal of Infectious Diseases. 191. (7). p. 1180-1187.

(2004). Antifungal activities of posaconazole and granulocyte-macrophage colony-stimulating factor ex vivo and in mice with disseminated infection due to Scedosporium prolificans . Antimicrobial Agents and Chemotherapy. 48. (10). p. 3801-3805.

(2003). Selective effects of interleukin (IL)-15 on antifungal activity and IL-8 release by polymorphonuclear leukocytes in response to hyphae of Aspergillus species . Journal of Infectious Diseases. 188. (4). p. 585-590.

(2000). Targeting exposed RNA regions in crystals of the small ribosomal subunits at medium resolution . Cellular and Molecular Biology. 46. (5). p. 871-882.

(1999). Partial purification and characterization of an aminopeptidase from Pediococcus pentosaceus K9.2 . Milchwissenschaft. 54. (9). p. 499-501.

(1999). Ribosomal gene disruption in the extreme thermophile Thermus thermophilus HB8. Generation of a mutant lacking ribosomal protein S17 . European Journal of Biochemistry. 266. (2). p. 524-532.

(1999). Structural and functional studies on the overproduced L11 protein from Thermus thermophilus . Journal of Protein Chemistry. 18. (2). p. 215-223.

(1998). Thermus Thermophilus L11 ribosomal protein: Cloning, overproduction, structural, and functional studies - In vivo methylation of the overproduced protein . Journal of Protein Chemistry. 17. (6). p. 519-520.

(1998). Thermus Thermophilus L11 Ribosomal Protein: Cloning, Overproduction, Structural, and Functional Studies - In Vivo Methylation of the Overproduced Protein . Protein Journal. 17. (6). p. 519-520.

Simitsopoulou, M., Vafopoulou, A., Choli-Papadopoulou, T., Alichanidis, E.(1997). Purification and partial characterization of a tripeptidase from Pediococcus pentosaceus K9.2 . Applied and Environmental Microbiology. 63. (12). p. 4872-4876.

Kyriazopoulou, V., Bondis, J., Frantzidou, F., Athanasiadis, A., Diza, E., Simitsopoulou, M., Souliou, E.(1996). Prenatal diagnosis of fetal cytomegalovirus infection in seropositive pregnant women . European Journal of Obstetrics Gynecology and Reproductive Biology. 69. (2). p. 91-95.

BOOK

(2016). Biofilms and antifungal susceptibility testing . Methods in Molecular Biology. 1356. p. 183-197.

(2007). Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi . Medical Mycology: Cellular and Molecular Techniques. p. 43-68.