Profile Details

Create Project

★★★★★

☆☆☆☆☆

USD 30 /hr

★★★★★

☆☆☆☆☆

Hire Dr. Mahadesh P.

India

USD 30 /hr

Profile Summary

I am commited and passionate researcher with 7+ years of post PhD research experience in cancer biology related domain. I have demonstrated the ability of working in multi disciplinary research areas and I have been part of number of research innovation. My research outputs have made advances in addressing key scientific question related to cancer biology, particularly, DNA damage associated cancers. Key Skills • Stem Cell Biology: Isolation of stem cells form bone marrow, liver, spleen and lymph nodes. Proficient in sorting. Capable of mimicking bone marrow microenvironment in cell culture. • Molecular Biology: Cloning, gene knockdown and over-expression techniques. Northern and southern techniques. Quantitative analysis of gene expression by qRT PCR, PCR. ChIP Seq. • Cell Biology: Mammalian cell culture techniques. Thoroughly acquainted with the maintenance of broad range of cell lines as well as clonal selection. • Microbiology and Virology: Extensive knowledge and hands on experience in typical bacteriology and virology techniques. Proficient in BSL-II/II+ laboratory. Knowledge of virus infection to B cells. • Protein Chemistry/ Proteomics: Protein Expression, Identification of interacting proteins by IP, Co-IP, GST pull down, mass spectrometry. • Microscopy: confocal microscope, fluorescence microscope and light microscope. • Spectroscopy: Multi color flowcytometry techniques and analysis. MALDI-TOF for identification of Microorganisms. • Bioinformatics: BLAST, and statistical analysis software such as Prism, graph pad and SPSS. • Lipid Chemistry: Synthesis of fatty acid fatty acid esters and secondary alcohol esters. • Animal Handling: Capable of handling laboratory rats, mice and rabbits. Diet administration, collecting blood tissue, bone marrow, spleen and lymph nodes. Please feel free to contact me via linkedin or email prasadjavarappa@hotmail.com

Subject Matter Expertise

• ☆ Mass Spectrometry
• ☆ Phytochemistry
• ☆ Virology
• ☆ Animal Biotechnology
• ☆ Biochemistry
• ☆ Molecular Biology
• ☆ PCR
• ☆ ChIP-Seq
• ☆ Stem Cell Research
• ☆ Microscopy
• ☆ Cell Culture
• ☆ Cell Aging
• ☆ SPSS
• ☆ Leukemia & Lymphoma

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Work Experience

Principal Investigator/ Assistant Professor

Department of Biochemistry, Pooja Bagavath Memorial PG Center. University of Mysore

August 2015 - Present

Postdoctoral researcher

University Hospital Linkoping, Linkoping, Sweden

February 2013 - June 2015

Postdoctoral fellow

University of Pennsylvania School of Medicine, Philadelphia, USA

November 2010 - December 2012

Visiting Scientist

University of Leipzig, Leipzig, Germany

August 2009 - November 2010

Education

PhD

University of Mysore

June 2005 - February 2010

MSc in Biochemistry

University of Mysore

June 2003 - June 2005

Certifications

• Certification details not provided.