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★★★★★
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USD 150 /hr
Hire Ludovico S.
Italy
USD 150 /hr

Passionate microscopists, who wants to extract the best from every imaging system

Profile Summary
Subject Matter Expertise
Services
Writing Technical Writing
Research Feasibility Study, Technology Scouting, Fact Checking
Consulting Scientific and Technical Consulting
Data & AI Image Processing, Image Analysis
Product Development Product Evaluation, Concept Development, Reverse Engineering
Work Experience

Assistant Professor

University of Florence

April 2019 - Present

Researcher

National Institute of Optics (INO-CNR)

January 2015 - March 2019

Post-doctoral research fellow

European Laboratory for Non-linear Spectroscopy

February 2012 - January 2015

Education

Ph.D. (European Laboratory for Non-linear Spectroscopy)

University of Florence

February 2009 - April 2012

Master (Department of Physics)

University of Pisa

October 2006 - October 2008

Bachelor (Department of Physics)

University of Pisa

October 2002 - October 2005

Certifications
  • Certification details not provided.
Publications
JOURNAL ARTICLE
3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy <head> <META HTTP-EQUIV="Refresh" CONTENT="0;URL=/servlet/useragent"> </head> . Communications Biology.
Ludovico Silvestri, Camilla Olianti, Francesco Giardini, Erica Lazzeri, Irene Costantini, Raffaele Coppini, Elisabetta Cerbai, Francesco S. Pavone, Leonardo Sacconi(2022). Optical clearing in cardiac imaging: A comparative study . Progress in Biophysics and Molecular Biology. Elsevier {BV}
L. Silvestri, M. C. M&#252;llenbroich, I. Costantini, A. P. Di Giovanna, G. Mazzamuto, A. Franceschini, D. Kutra, A. Kreshuk, C. Checcucci, L. O. Toresano, et al.(2021). Universal autofocus for quantitative volumetric microscopy of whole mouse brains . Nature Methods. Springer Science and Business Media {LLC}
Ricci, P., Sancataldo, G., Gavryusev, V., Franceschini, A., M&#252;llenbroich, M.C., Silvestri, L., Pavone, F.S.(2020). Fast multi-directional DSLM for confocal detection without striping artifacts . Biomedical Optics Express. 11. (6). p. 3111-3124.
(2019). Advanced fluorescence microscopy for in vivo imaging of neuronal activity . Optica.
Ludovico Silvestri, Antonino Paolo Di Giovanna, Alessandro Tibo, Marie Caroline M&#252;llenbroich, Irene Costantini, Anna Letizia Allegra Mascaro, Leonardo Sacconi, Paolo Frasconi, Francesco Saverio Pavone (2019). Publisher Correction: Whole-Brain Vasculature Reconstruction at the Single Capillary Level . Scientific Reports.
Gavryusev, V., Sancataldo, G., Ricci, P., Montalbano, A., Fornetto, C., Turrini, L., Laurino, A., Pesce, L., De Vito, G., Tiso, N., et al.(2019). Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector . Journal of Biomedical Optics. 24. (10).
Di Giovanna, A.P., Tibo, A., Silvestri, L., M&#252;llenbroich, M.C., Costantini, I., Allegra Mascaro, A.L., Sacconi, L., Frasconi, P., Pavone, F.S.(2019). Publisher Correction: Whole-Brain Vasculature Reconstruction at the Single Capillary Level (Scientific Reports, (2018), 8, 1, (12573), 10.1038/s41598-018-30533-3) . Scientific Reports. 9. (1).
Costantini, I., Cicchi, R., Silvestri, L., Vanzi, F., Pavone, F.S.(2019). In-vivo and ex-vivo optical clearing methods for biological tissues: Review . Biomedical Optics Express. 10. (10). p. 5251-5267.
M&#252;llenbroich, M.C., Turrini, L., Silvestri, L., Alterini, T., Gheisari, A., Tiso, N., Vanzi, F., Sacconi, L., Pavone, F.S.(2019). Corrigendum: Bessel beam illumination reduces random and systematic errors in quantitative functional studies using light-sheet microscopy (frontiers in cellular neuroscience, (2018), 12, 10.3389/fncel.2018.00315) . Frontiers in Cellular Neuroscience. 13. p. 1-1.
Di Giovanna, A.P., Credi, C., Franceschini, A., M&#252;llenbroich, M.C., Silvestri, L., Pavone, F.S.(2019). Tailored sample mounting for light-sheet fluorescence microscopy of clarified specimens by polydimethylsiloxane casting . Frontiers in Neuroanatomy. 13.
Sancataldo, G., Silvestri, L., Mascaro, A.L.A., Sacconi, L., Pavone, A.F.S.(2019). Advanced fluorescence microscopy for in vivo imaging of neuronal activity . Optica. 6. (6). p. 758-765.
Sancataldo, G., Gavryusev, V., de Vito, G., Turrini, L., Locatelli, M., Fornetto, C., Tiso, N., Vanzi, F., Silvestri, L., Pavone, F.S.(2019). Flexible multi-beam light-sheet fluorescence microscope for live imaging without striping artifacts . Frontiers in Neuroanatomy. 13.
Ludovico Silvestri, Antonino Paolo Di Giovanna, Alessandro Tibo, Marie Caroline M&#252;llenbroich, Irene Costantini, Anna Letizia Allegra Mascaro, Leonardo Sacconi, Paolo Frasconi, Francesco Saverio Pavone (2018). Whole-Brain Vasculature Reconstruction at the Single Capillary Level . Scientific Reports.
M&#252;llenbroich, M.C., Turrini, L., Silvestri, L., Alterini, T., Gheisari, A., Vanzi, F., Sacconi, L., Pavone, F.S.(2018). Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy . Frontiers in Cellular Neuroscience. 12.
Adam, M.-P., M&#252;llenbroich, M.C., Di Giovanna, A.P., Alfieri, D., Silvestri, L., Sacconi, L., Pavone, F.S.(2018). Confocal multispot microscope for fast and deep imaging in semicleared tissues . Journal of Biomedical Optics. 23. (2).
Bjerke, I.E., &#216;vsthus, M., Papp, E.A., Yates, S.C., Silvestri, L., Fiorilli, J., Pennartz, C.M.A., Pavone, F.S., Puchades, M.A., Leergaard, T.B., et al.(2018). Data integration through brain atlasing: Human Brain Project tools and strategies . European Psychiatry. 50. p. 70-76.
M&#252;llenbroich, M.C., Silvestri, L., Di Giovanna, A.P., Mazzamuto, G., Costantini, I., Sacconi, L., Pavone, F.S.(2018). High-fidelity imaging in brain-wide structural studies using light-sheet microscopy . eNeuro. 5. (6).
Scardigli, M., Crocini, C., Ferrantini, C., Gabbrielli, T., Silvestri, L., Coppini, R., Tesi, C., Rog-Zielinska, E.A., Kohl, P., Cerbai, E., et al.(2018). Reply to entcheva: The impact of T-tubules on action potential propagation in cardiac tissue . Proceedings of the National Academy of Sciences of the United States of America. 115. (4). p. E562-E563.
Di Giovanna, A.P., Tibo, A., Silvestri, L., M&#252;llenbroich, M.C., Costantini, I., Allegra Mascaro, A.L., Sacconi, L., Frasconi, P., Pavone, F.S.(2018). Whole-Brain Vasculature Reconstruction at the Single Capillary Level . Scientific Reports. 8. (1).
Scardigli, M., Crocini, C., Ferrantini, C., Gabbrielli, T., Silvestri, L., Coppini, R., Tesi, C., Rog-Zielinska, E.A., Kohl, P., Cerbai, E., et al.(2017). Quantitative assessment of passive electrical properties of the cardiac T-tubular system by FRAP microscopy . Proceedings of the National Academy of Sciences of the United States of America. 114. (22). p. 5737-5742.
Silvestri, L., Costantini, I., Sacconi, L., Pavone, F.S.(2016). Clearing of fixed tissue: A review from a microscopist's perspective . Journal of Biomedical Optics. 21. (8).
Allegra Mascaro, A.L., Sacconi, L., Silvestri, L., Knott, G., Pavone, F.S.(2016). Multi-Modal Optical Imaging of the Cerebellum in Animals . Cerebellum. 15. (1). p. 18-20.
(2015). Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains . Neurophotonics.
M&#252;llenbroich, M.C., Silvestri, L., Onofri, L., Costantini, I., Van&#39;t Hoff, M., Sacconi, L., Iannello, G., Pavone, F.S.(2015). Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains . Neurophotonics. 2. (4).
Costantini, I., Ghobril, J.-P., Di Giovanna, A.P., Mascaro, A.L.A., Silvestri, L., M&#252;llenbroich, M.C., Onofri, L., Conti, V., Vanzi, F., Sacconi, L., et al.(2015). A versatile clearing agent for multi-modal brain imaging . Scientific Reports. 5.
Silvestri, L., Paciscopi, M., Soda, P., Biamonte, F., Iannello, G., Frasconi, P., Pavone, F.S.(2015). Quantitative neuroanatomy of all Purkinje cells with light sheet microscopy and high-throughput image analysis . Frontiers in Neuroanatomy. 9. (MAY).
Allegra Mascaro, A.L., Silvestri, L., Sacconi, L., Pavone, F.S.(2015). Towards a comprehensive understanding of brain machinery by correlative microscopy . Journal of Biomedical Optics. 20. (6).
Silvestri, L., Sacconi, L., Pavone, F.S.(2014). Correcting spherical aberrations in confocal light sheet microscopy: A theoretical study . Microscopy Research and Technique. 77. (7). p. 483-491.
Silvestri, L., Allegra Mascaro, A.L., Costantini, I., Sacconi, L., Pavone, F.S.(2014). Correlative two-photon and light sheet microscopy . Methods. 66. (2). p. 268-272.
Frasconi, P., Silvestri, L., Soda, P., Cortini, R., Pavone, F.S., Iannello, G.(2014). Large-scale automated identification of mouse brain cells in confocal light sheet microscopy images . Bioinformatics. 30. (17).
Silvestri, L., Mascaro, A.L.A., Lotti, J., Sacconi, L., Pavone, F.S.(2013). Advanced optical techniques to explore brain structure and function . Journal of Innovative Optical Health Sciences. 6. (1).
Allegra Mascaro, A.L., Silvestri, L., Sacconi, L., Pavone, F.S.(2013). Breakthroughs in photonics 2012: Nonlinear laser imaging for neuroscience . IEEE Photonics Journal. 5. (2).
Silvestri, L., Bria, A., Costantini, I., Sacconi, L., Peng, H., Iannello, G., Pavone, F.S.(2013). Micron-scale resolution optical tomography of entire mouse brains with confocal light sheet microscopy . Journal of visualized experiments : JoVE. (80).
Silvestri, L., Sacconi, L., Pavone, F.S.(2013). The connectomics challenge . Functional Neurology. 28. (3). p. 167-173.
Silvestri, L., Bria, A., Sacconi, L., Iannello, G., Pavone, F.S.(2012). Confocal light sheet microscopy: Micron-scale neuroanatomy of the entire mouse brain . Optics Express. 20. (18). p. 20582-20598.
Silvestri, L., Fronzoni, L., Grigolini, P., Allegrini, P.(2009). Event-driven power-law relaxation in weak turbulence . Physical Review Letters. 102. (1).
Allegrini, P., Bologna, M., Fronzoni, L., Grigolini, P., Silvestri, L.(2009). Experimental quenching of harmonic stimuli: Universality of linear response theory . Physical Review Letters. 103. (3).
OTHER
Ludovico Silvestri, Luca Pesce, Marina Scardigli, Vladislav Gavryusev, Annunziatina Laurino, Giacomo Mazzamuto, Giuseppe Sancataldo, Christophe Destrieux, Patrick R. Hof, Irene Costantini, et al.(2021). 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy . Cold Spring Harbor Laboratory
CONFERENCE PAPER
Pesce, L., Laurino, A., Gavryusev, V., Mazzamuto, G., Sancataldo, G., Scardigli, M., Roffilli, M., Silvestri, L., Costantini, I., Pavone, F.S.(2020). Fast volumetric mapping of human brain slices . Proceedings of SPIE - The International Society for Optical Engineering. 11360.
De Vito, G., Fornetto, C., Ricci, P., M&#252;llenbroich, C., Sancataldo, G., Turrini, L., Mazzamuto, G., Tiso, N., Sacconi, L., Fanelli, D., et al.(2020). Two-photon high-speed light-sheet volumetric imaging of brain activity during sleep in zebrafish larvae . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 11226.
De Vito, G., Turrini, L., Fornetto, C., Ricci, P., M&#252;llenbroich, C., Sancataldo, G., Trabalzini, E., Mazzamuto, G., Tiso, N., Sacconi, L., et al.(2020). Two-photon light-sheet microscopy for high-speed whole-brain functional imaging of zebrafish neuronal physiology and pathology . Proceedings of SPIE - The International Society for Optical Engineering. 11360.
Pesce, L., Laurino, A., Gavryusev, V., Mazzamuto, G., Sancataldo, G., Scardigli, M., Roffilli, M., Silvestri, L., Costantini, I., Pavone, F.S.(2020). Fast volumetric mapping of human brain slices . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 11226.
Mazzamuto, G., Orsini, F., Roffilli, M., Frasconi, P., Pavone, F.S., Silvestri, L.(2019). Deep learning strategies for scalable analysis of high-resolution brain imagery . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 11076.
Costantini, I., Mazzamuto, G., Laurino, A., Lazzeri, E., Sacconi, L., Neri, M., Simonetto, A., Roffilli, M., Silvestri, L., Pavone, F.S.(2019). Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 11076.
Costantini, I., Mazzamuto, G., Silvestri, L., Neri, M., Roffilli, M., Conti, V., Guerrini, R., Sacconi, L., Pavone, F.S.(2018). 3D human brain digital istopatology . Optics InfoBase Conference Papers. Part F88-BRAIN 2018.
Mazzamuto, G., Silvestri, L., Costantini, I., Orsini, F., Roffilli, M., Frasconi, P., Sacconi, L., Pavone, F.S.(2018). Software tools for efficient processing of high-resolution 3D images of macroscopic brain samples . Optics InfoBase Conference Papers. Part F91-TRANSLATIONAL 2018.
Silvestri, L., Di Giovanna, A.P., Mazzamuto, G., Leergard, T., Orsini, F., Costantini, I., Bjaalie, J., Frasconi, P., Pavone, F.S.(2018). Towards a full volumetric atlas of cell-specific neuronal spatial organization in the entire mouse brain . Optics InfoBase Conference Papers. Part F91-TRANSLATIONAL 2018.
Costantini, I., Menzel, M., Silvestri, L., Schubert, N., Axer, M., Amunts, K., Pavone, F.S.(2017). Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction . Optics InfoBase Conference Papers. Part F61-ECBO 2017.
Silvestri, L., M&#252;llenbroich, M.C., Costantini, I., Di Giovanna, A.P., Sacconi, L., Pavone, F.S.(2017). Fast, image-based autofocus system for high-resolution optical microscopy of whole mouse brains . Optics InfoBase Conference Papers. Part F62-BODA 2017.
Mu&#168;llenbroich, M.C., Silvestri, L., Turrini, L., Di Giovanna, A.P., Alterini, T., Gheisari, A., Sacconi, L., Vanzi, F., Pavone, F.S.(2017). High-fidelity imaging with bessel-beam light-sheet microscopy for whole-brain structural and functional studies . Optics InfoBase Conference Papers. Part F76-BRAIN 2017.
Di Giovanna, A.P., Tibo, A., Silvestri, L., M&#252;llenbroich, M.C., Costantini, I., Sacconi, L., Frasconi, P., Pavone, F.S.(2017). Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy . Optics InfoBase Conference Papers. Part F61-ECBO 2017.
Costantini, I., Menzel, M., Silvestri, L., Schubert, N., Axer, M., Amunts, K., Pavone, F.S.(2017). Polarized light imaging and two-photon fluorescence microscopy correlative approach for 3D reconstruction of the orientation of myelinated fibers . Optics InfoBase Conference Papers. Part F76-BRAIN 2017.
Turrini, L., Alterini, T., M&#252;llenbroich, C., Gheisari, A., Sacconi, L., Silvestri, L., Vanzi, F., Pavone, F.S.(2016). Functional imaging of zebrafish neuronal activity by Bessel beam light-sheet microscopy . IET Conference Publications. 2016. (CP704).
Costantini, I., Mascaro, A.L.A., Di Giovanna, A.P., Silvestri, L., M&#252;llenbroich, M.C., Sacconi, L., Pavone, F.S.(2015). A multi modal clearing method for brain imaging . Optics and the Brain, BRAIN 2015.
Costantini, I., Mascaro, A.L.A., Di Giovanna, A.P., Silvestri, L., M&#252;llenbroich, M.C., Sacconi, L., Pavone, F.S.(2015). A multi modal clearing method for brain imaging . Optics and the Brain, BRAIN 2015.
Costantini, I., Di Giovanna, A.P., Allegra Mascaro, A.L., Silvestri, L., M&#252;llenbroich, M.C., Sacconi, L., Pavone, F.S.(2015). A new versatile clearing method for brain imaging . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 9305.
Silvestri, L., Rudinskiy, N., Paciscopi, M., M&#252;llenbroich, M.C., Costantini, I., Sacconi, L., Frasconi, P., Hyman, B.T., Pavone, F.S.(2015). Brain-wide charting of neuronal activation maps with cellular resolution . Optics and the Brain, BRAIN 2015.
Silvestri, L., Rudinskiy, N., Paciscopi, M., M&#252;llenbroich, M.C., Costantini, I., Sacconi, L., Frasconi, P., Hyman, B.T., Pavone, F.S.(2015). Brain-wide charting of neuronal activation maps with cellular resolution . Optics and the Brain, BRAIN 2015.
Silvestri, L., Allegra Mascaro, A.L., Costantini, I., Sacconi, L., Pavone, F.S.(2015). Brain imaging from the nano- to the macro-scale . International IEEE/EMBS Conference on Neural Engineering, NER. 2015-July. p. 864-866.
Sacconi, L., Mascaro, A.L.A., Silvestri, L., Pavone, F.S.(2015). Towards a comprehensive understanding of brain structure by correlative microscopy . Optics and the Brain, BRAIN 2015.
Sacconi, L., Mascaro, A.L.A., Silvestri, L., Pavone, F.S.(2015). Towards a comprehensive understanding of brain structure by correlative microscopy . Optics and the Brain, BRAIN 2015.
Silvestri, L., Allegra Mascaro, A.L., Costantini, I., Sacconi, L., Pavone, F.S.(2015). Whole brain optical imaging . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 9305.
Allegra Mascaro, A.L., Silvestri, L., Costantini, I., Sacconi, L., Maco, B., Knott, G.W., Pavone, F.S.(2015). Multiphoton microscopy in brain imaging . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 9329.
Costantini, I., Di Giovanna, A.P., Mascaro, A.L.A., Silvestri, L., M&#252;llenbroich, M.C., Sacconi, L., Pavone, F.S.(2014). A versatile new technique to clear mouse and human brain . Optics InfoBase Conference Papers.
Silvestri, L., Allegra Mascaro, A.L., Costantini, I., Sacconi, L., Pavone, F.S.(2014). Exploring the brain on multiple scales with correlative two-photon and light sheet microscopy . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 8948.
Bria, A., Iannello, G., Soda, P., Peng, H., Erbacci, G., Fiameni, G., Mariani, G., Mucci, R., Rorro, M., Pavone, F., et al.(2014). A HPC infrastructure for processing and visualizing neuro-anatomical images obtained by Confocal Light Sheet Microscopy . Proceedings of the 2014 International Conference on High Performance Computing and Simulation, HPCS 2014. p. 592-599.
Silvestri, L., Sacconi, L., Pavone, F.S.(2013). Light sheet microscopy of cleared mouse brains: Aberrations effects caused by refractive index mismatch . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 8804.
Allegra Mascaro, A.L., Silvestri, L., Costantini, I., Sacconi, L., Maco, B., Knott, G.W., Pavone, F.S.(2013). Neural plasticity explored by correlative two-photon and electron/SPIM microscopy . Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 8797.
Mascaro, A.L.Allegra, Silvestri, L., Costantini, I., Sacconi, L., Maco, B., Knott, G.W., Pavone, F.S.(2013). Neural plasticity explored by correlative two-photon and electron/spim microscopy . Optics InfoBase Conference Papers.
Silvestri, L., Sacconi, L., Pavone, F.S.(2013). Light sheet microscopy of cleared mouse brains: Aberrations effects caused by refractive index mismatch . Optics InfoBase Conference Papers.
Bria, A., Silvestri, L., Sacconi, L., Pavone, F., Iannello, G.(2012). Stitching terabyte-sized 3D images acquired in confocal ultramicroscopy . Proceedings - International Symposium on Biomedical Imaging. p. 1659-1662.
Silvestri, L., Fronzoni, L., Gemignani, A., Grigolini, P., Menicucci, D., Allegrini, P.(2009). Event-driven power-law relaxation in weak turbulence: A liquid crystals mesoscopic experiment bridging quantum dots and the integration theory for the brain . Journal of Physics: Conference Series. 174. (1).
BOOK CHAPTER
(2018). Automatic Segmentation of Neurons in 3D Samples of Human Brain Cortex . Applications of Evolutionary Computation.
Mascaro, A.L.A., Silvestri, L., Sacconi, L., Pavone, F.S.(2018). Multiscale correlative imaging of the brain . Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine. p. 321-344.
BOOK
Mazzamuto, G., Costantini, I., Neri, M., Roffilli, M., Silvestri, L., Pavone, F.S.(2018). Automatic Segmentation of Neurons in 3D Samples of Human Brain Cortex . Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). 10784 LNCS. p. 78-85.