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Hire Dr. Leonard E.

United States

USD 100 /hr

20 years experience in pharmaceutical and academic research with expertise in genomics, gene editing, and hematology.

Profile Summary

Accomplished scientific leader in functional genomics with experience in biomarker analysis, pharmacogenetics, molecular biology, gene editing tools, cell therapy development, target discovery, CAR-T and T-cell engager analysis, virology, oncology, and immunology. Proven track record in managing cross-functional teams, fostering partnerships and delivering critical data to advance biopharmaceutical science. Successful application of CRISPR/genome editing screens for Target Identification and Validation, awarded NIH and AHA funding. Finalist for AHA Brinkhous Early Career Investigator Award in Thrombosis.

Subject Matter Expertise

• ☆ Microbiology
• ☆ Virology
• ☆ Molecular Virology
• ☆ Biochemistry
• ☆ Biochemical Assays
• ☆ Molecular Biology
• ☆ Genetics
• ☆ Omics
• ☆ Transcriptomics
• ☆ Genomics
• ☆ Epigenetics
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cell Signaling Pathways
• ☆ Gene Regulation
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Biomolecular Engineering
• ☆ Gene Editing/CRISPR
• ☆ Drug Development
• ☆ Drug Substance Characterization
• ☆ Hematology
• ☆ Hematology-Oncology
• ☆ Medical Writing
• ☆ Research Summaries & Abstracts
• ☆ Grant Writing

Services

• Writing
• Research
• Consulting

Writing Technical Writing

Research Technology Scouting, Fact Checking, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Work Experience

Janssen Research and Development Spring House

May 2020 - Present

Assistant Professor

Thomas Jefferson University

October 2010 - May 2020

Adjunct Assistant Professor

Rutgers Robert Wood Johnson Medical School

October 2006 - July 2010

Instructor

Harvard Medical School

May 2005 - July 2006

Postdoctoral Fellow

Harvard Medical School

July 2002 - April 2005

Graduate Student

Rutgers Robert Wood Johnson Medical School

September 1996 - June 2002

Education

(Pathology)

Harvard Medical School

July 2002 - April 2005

PhD (Biochemisty)

Rutgers University - Busch Campus

August 1996 - June 2002

BS (Biology)

Cornell University

August 1992 - May 1996

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2022). Human and mouse PAR4 are functionally distinct receptors: Studies in novel humanized mice . Journal of thrombosis and haemostasis : JTH.

(2021). PCTP contributes to human platelet activation by enhancing dense granule secretion . Thrombosis research.

Seema Bhatlekar, Bhanu K. Manne, Indranil Basak, Leonard C. Edelstein, Emilia Tugolukova, Michelle L. Stoller, Mark J. Cody, Sharon C. Morley, Srikanth Nagalla, Andrew S. Weyrich, et al.(2020). miR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein L-plastin . Blood. American Society of Hematology

Andrew R. Ghazi, Jian Ma, Xianguo Kong, Ed S. Chen, Leonard C. Edelstein, Chad A. Shaw(2020). Bayesian modelling of high-throughput sequencing assays with malacoda . PLOS Computational Biology. 16. (7). p. e1007504. Public Library of Science ({PLoS})

Namrata Madan, Folkert Wouter Asselbergs, Andrew R. Ghazi, Xianguo Kong, Edward S. Chen, Chad A. Shaw, Leonard C. Edelstein(2019). Functionalization of CD36 cardiovascular disease and expression associated variants by interdisciplinary high throughput analysis . PLOS Genetics. 15. (7). p. e1008287. Public Library of Science ({PLoS})

Lawrence E. Goldfinger, Leonard C. Edelstein (2019). Horizontal RNA transfer goes deep: platelet consumption and microRNA utilization by vascular smooth muscle cells . Journal of Thrombosis and Haemostasis.

Basak I, Bhatlekar S, Manne BK, Stoller M, Hugo S, Kong X, Ma L, Rondina MT, Weyrich AS, Edelstein LC, et al.(2019). miR-15a-5p regulates expression of multiple proteins in the megakaryocyte GPVI signaling pathway . Journal of thrombosis and haemostasis : JTH.

Basak, I., Bhatlekar, S., Manne, B.K., Stoller, M., Hugo, S., Kong, X., Ma, L., Rondina, M.T., Weyrich, A.S., Edelstein, L.C., et al.(2019). miR-15a-5p regulates expression of multiple proteins in the megakaryocyte GPVI signaling pathway . Journal of Thrombosis and Haemostasis. 17. (3). p. 511-524.

Goldfinger, L.E., Edelstein, L.C.(2019). Horizontal RNA transfer goes deep: platelet consumption and microRNA utilization by vascular smooth muscle cells . Journal of Thrombosis and Haemostasis. 17. (7). p. 1014-1017.

Kong, X., Ma, L., Chen, E., Shaw, C.A., Edelstein, L.C.(2019). Identification of the Regulatory Elements and Target Genes of Megakaryopoietic Transcription Factor MEF2C . Thrombosis and Haemostasis. 119. (5). p. 716-725.

Whitley MJ, Henke DM, Ghazi A, Nieman M, Stoller M, Simon LM, Chen E, Vesci J, Holinstat M, McKenzie SE, et al.(2018). The protease-activated receptor 4 Ala120Thr variant alters platelet responsiveness to low-dose thrombin and protease-activated receptor 4 desensitization, and is blocked by non-competitive P2Y12 inhibition . Journal of thrombosis and haemostasis : JTH.

Ghazi AR, Chen ES, Henke DM, Madan N, Edelstein LC, Shaw CA(2018). Design tools for MPRA experiments . Bioinformatics (Oxford, England).

Tricoci P, Neely M, Whitley MJ, Edelstein LC, Simon LM, Shaw C, Fortina P, Moliterno DJ, Armstrong PW, Aylward P, et al.(2018). Effects of genetic variation in protease activated receptor 4 after an acute coronary syndrome: Analysis from the TRACER trial . Blood cells, molecules & diseases.

Tourdot BE, Stoveken H, Trumbo D, Yeung J, Kanthi Y, Edelstein LC, Bray PF, Tall GG, Holinstat M(2018). Genetic Variant in Human PAR (Protease-Activated Receptor) 4 Enhances Thrombus Formation Resulting in Resistance to Antiplatelet Therapeutics . Arteriosclerosis, thrombosis, and vascular biology.

Tourdot, B.E., Stoveken, H., Trumbo, D., Yeung, J., Kanthi, Y., Edelstein, L.C., Bray, P.F., Tall, G.G., Holinstat, M.(2018). Genetic variant in human PAR (Protease-Activated Receptor) 4 enhances thrombus formation resulting in resistance to antiplatelet therapeutics . Arteriosclerosis, Thrombosis, and Vascular Biology. 38. (7). p. 1632-1643.

Tricoci, P., Neely, M., Whitley, M.J., Edelstein, L.C., Simon, L.M., Shaw, C., Fortina, P., Moliterno, D.J., Armstrong, P.W., Aylward, P., et al.(2018). Effects of genetic variation in protease activated receptor 4 after an acute coronary syndrome: Analysis from the TRACER trial . Blood Cells, Molecules, and Diseases. 72. p. 37-43.

Whitley, M.J., Henke, D.M., Ghazi, A., Nieman, M., Stoller, M., Simon, L.M., Chen, E., Vesci, J., Holinstat, M., McKenzie, S.E., et al.(2018). The protease-activated receptor 4 Ala120Thr variant alters platelet responsiveness to low-dose thrombin and protease-activated receptor 4 desensitization, and is blocked by non-competitive P2Y<inf>12</inf> inhibition . Journal of Thrombosis and Haemostasis. 16. (12). p. 2501-2514.

Ghazi, A.R., Chen, E.S., Henke, D.M., Madan, N., Edelstein, L.C., Shaw, C.A.(2018). Design tools for MPRA experiments . Bioinformatics (Oxford, England). 34. (15). p. 2682-2683.

Michael JV, Wurtzel JGT, Mao GF, Rao AK, Kolpakov MA, Sabri A, Hoffman NE, Rajan S, Tomar D, Madesh M, et al.(2017). Platelet microparticles infiltrating solid tumors transfer miRNAs that suppress tumor growth . Blood.

Kong X, Simon LM, Holinstat M, Shaw CA, Bray PF, Edelstein LC(2017). Identification of a functional genetic variant driving racially dimorphic platelet gene expression of the thrombin receptor regulator, PCTP . Thrombosis and haemostasis.

Kong, X., Simon, L.M., Holinstat, M., Shaw, C.A., Bray, P.F., Edelstein, L.C.(2017). Identification of a functional genetic variant driving racially dimorphic platelet gene expression of the thrombin receptor regulator, PCTP . Thrombosis and Haemostasis. 117. (5). p. 962-970.

Edelstein, L.C.(2017). The role of platelet microvesicles in intercellular communication . Platelets. 28. (3). p. 222-227.

Michael, J.V., Wurtzel, J.G.T., Mao, G.F., Rao, A.K., Kolpakov, M.A., Sabri, A., Hoffman, N.E., Rajan, S., Tomar, D., Madesh, M., et al.(2017). Platelet microparticles infiltrating solid tumors transfer miRNAs that suppress tumor growth . Blood. 130. (5). p. 567-580.

Edelstein LC(2016). The role of platelet microvesicles in intercellular communication . Platelets.

Simon, L.M., Chen, E.S., Edelstein, L.C., Kong, X., Bhatlekar, S., Rigoutsos, I., Bray, P.F., Shaw, C.A.(2016). Integrative Multi-omic Analysis of Human Platelet eQTLs Reveals Alternative Start Site in Mitofusin 2 . American Journal of Human Genetics. 98. (5). p. 883-897.

Lindsay, C.R., Edelstein, L.C.(2016). MicroRNAs in platelet physiology and function . Seminars in Thrombosis and Hemostasis. 42. (3). p. 215-222.

Zhou, Y., Abraham, S., Renna, S., Edelstein, L.C., Dangelmaier, C.A., Tsygankov, A.Y., Kunapuli, S.P., Bray, P.F., McKenzie, S.E.(2016). TULA-2 (T-Cell Ubiquitin Ligand-2) Inhibits the Platelet Fc Receptor for IgG IIA (FcγRIIA) Signaling Pathway and Heparin-Induced Thrombocytopenia in Mice . Arteriosclerosis, Thrombosis, and Vascular Biology. 36. (12). p. 2315-2323.

Yuhang Zhou, Shaji Abraham, Pierrette Andre, Leonard C. Edelstein, Chad A. Shaw, Carol A. Dangelmaier, Alexander Y. Tsygankov, Satya P. Kunapuli, Paul F. Bray, Steven E. McKenzie(2015). Anti–miR-148a regulates platelet FcγRIIA signaling and decreases thrombosis in vivo in mice . Blood. 126. (26). p. 2871--2881. American Society of Hematology

Zhou, Y., Abraham, S., Andre, P., Edelstein, L.C., Shaw, C.A., Dangelmaier, C.A., Tsygankov, A.Y., Kunapuli, S.P., Bray, P.F., McKenzie, S.E.(2015). Anti-miR-148a regulates platelet FcγRIIA signaling and decreases thrombosis in vivo in mice . Blood. 126. (26). p. 2871-2881.

et al., Edelstein, L.C., Simon, L.M., Lindsay, C.R., Kong, X., Teruel-Montoya, R., Tourdot, B.E., Chen, E.S., Ma, L., Coughlin, S., Nieman, M.(2014). Common variants in the human platelet PAR4 thrombin receptor alter platelet function and differ by race . Blood. 124. (23). p. 3450-3458.

Simon, L.M., Edelstein, L.C., Nagalla, S., Woodley, A.B., Chen, E.S., Kong, X., Ma, L., Fortina, P., Kunapuli, S., Holinstat, M., et al.(2014). Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics . Blood. 123. (16).

Tourdot, B.E., Conaway, S., Niisuke, K., Edelstein, L.C., Bray, P.F., Holinstat, M.(2014). Mechanism of race-dependent platelet activation through the protease-activated receptor-4 and Gq signaling axis . Arteriosclerosis, Thrombosis, and Vascular Biology. 34. (12). p. 2644-2650.

Teruel-Montoya, R., Kong, X., Abraham, S., Ma, L., Kunapuli, S.P., Holinstat, M., Shaw, C.A., McKenzie, S.E., Edelstein, L.C., Bray, P.F.(2014). MicroRNA expression differences in human hematopoietic cell lineages enable regulated transgene expression . PLoS ONE. 9. (7).

Londin, E.R., Hatzimichael, E., Loher, P., Edelstein, L., Shaw, C., Delgrosso, K., Fortina, P., Bray, P.F., McKenzie, S.E., Rigoutsos, I.(2014). The human platelet: Strong transcriptome correlations among individuals associate weakly with the platelet proteome . Biology Direct. 9. (1).

Edelstein, L.C., Mckenzie, S.E., Shaw, C., Holinstat, M.A., Kunapuli, S.P., Bray, P.F.(2013). MicroRNAs in platelet production and activation . Journal of Thrombosis and Haemostasis. 11. (SUPPL.1). p. 340-350.

Edelstein, L.C., Simon, L.M., Montoya, R.T., Holinstat, M., Chen, E.S., Bergeron, A., Kong, X., Nagalla, S., Mohandas, N., Cohen, D.E., et al.(2013). Racial differences in human platelet PAR4 reactivity reflect expression of PCTP and miR-376c . Nature Medicine. 19. (12). p. 1609-1616.

Edelstein LC, Bray PF(2012). Small RNAs as potential platelet therapeutics . Handbook of experimental pharmacology.

Edelstein, L.C., Luna, E.J., Gibson, I.B., Bray, M., Jin, Y., Kondkar, A., Nagalla, S., Hadjout-Rabi, N., Smith, T.C., Covarrubias, D., et al.(2012). Human genome-wide association and mouse knockout approaches identify platelet supervillin as an inhibitor of thrombus formation under shear stress . Circulation. 125. (22). p. 2762-2771.

Edelstein, L.C., Bray, P.F.(2011). MicroRNAs in platelet production and activation . Blood. 117. (20). p. 5289-5296.

Nagalla, S., Shaw, C., Kong, X., Kondkar, A.A., Edelstein, L.C., Ma, L., Chen, J., McKnight, G.S., L&#243;pez, J.A., Yang, L., et al.(2011). Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity . Blood. 117. (19). p. 5189-5197.

Micheva-Viteva, S., Kobayashi, Y., Edelstein, L.C., Pacchia, A.L., Lee, H.-L.R., Graci, J.D., Breslin, J., Phelan, B.D., Miller, L.K., Colacino, J.M., et al.(2011). High-throughput screening uncovers a compound that activates latent HIV-1 and acts cooperatively with a histone deacetylase (HDAC) inhibitor . Journal of Biological Chemistry. 286. (24). p. 21083-21091.

Edelstein, L.C., Micheva-Viteva, S., Phelan, B.D., Dougherty, J.P.(2009). Short communication: Activation of latent HIV type 1 gene expression by suberoylanilide hydroxamic acid (SAHA), an HDAC inhibitor approved for use to treat cutaneous T cell lymphoma . AIDS Research and Human Retroviruses. 25. (9). p. 883-887.

Edelstein, L.C., Collins, T.(2005). The SCAN domain family of zinc finger transcription factors . Gene. 359. (1-2). p. 1-17.

Edelstein, L.C., Pan, A., Collins, T.(2005). Chromatin Modification and the Endothelial-specific activation of the E-selectin gene . Journal of Biological Chemistry. 280. (12). p. 11192-11202.

D&#39;Souza, B.N., Edelstein, L.C., Pegman, P.M., Smith, S.M., Loughran, S.T., Clarke, A., Mehl, A., Rowe, M., G&#233;linas, C., Walls, D.(2004). Nuclear Factor κB-Dependent Activation of the Antiapoptotic bfl-1 Gene by the Epstein-Barr Virus Latent Membrane Protein 1 and Activated CD40 Receptor . Journal of Virology. 78. (4). p. 1800-1816.

Edelstein, L.C., Lagos, L., Simmons, M., Tirumalai, H., G&#233;linas, C.(2003). NF-κB-dependent assembly of an enhanceosome-like complex on the promoter region of apoptosis inhibitor Bfl-1/A1 . Molecular and Cellular Biology. 23. (8). p. 2749-2761.

Suh, J., Payvandi, F., Edelstein, L.C., Amenta, P.S., Zong, W.-X., G&#233;linas, C., Rabson, A.B.(2002). Mechanisms of constitutive NF-κB activation in human prostate cancer cells . Prostate. 52. (3). p. 183-200.

Chen, C., Edelstein, L.C., G&#233;linas, C.(2000). The Rel/NF-κb family directly activates expression of the apoptosis inhibitor BCl-x(L) . Molecular and Cellular Biology. 20. (8). p. 2687-2695.

Zong, W.-X., Edelstein, L.C., Chen, C., Bash, J., G&#233;linas, C.(1999). The prosurvival Bcl-2 homolog Bfl-1/A1 is a direct transcriptional target of NF-κB that blocks TNFα-induced apoptosis . Genes and Development. 13. (4). p. 382-387.

OTHER

Namrata Madan, Andrew R. Ghazi, Xianguo Kong, Edward S. Chen, Chad A. Shaw, Leonard C. Edelstein(2019). Identification of functional variants for platelet CD36 expression by Massively Parallel Reporter Assay . Cold Spring Harbor Laboratory

Claire Simonneau, Junning Yang, Xianguo Kong, Robert Kilker, Leonard Edelstein, Paolo Fortina, Eric Londin, Arie Horowitz(2018). Validation of a Miniaturized Permeability Assay Compatible with CRISPR-Mediated Genome-Wide Screen . Cold Spring Harbor Laboratory

Simonneau C, Yang J, Kong X, Kilker R, Edelstein L, Fortina P, Londin E, Horowitz A(2018). Miniaturized Permeability Assay for CRISPR-Mediated Genome-Wide Screens .

Bray, P.F., McKenzie, S.E., Edelstein, L.C., Nagalla, S., Delgrosso, K., Ertel, A., Kupper, J., Jing, Y., Londin, E., Loher, P., et al.(2013). The complex transcriptional landscape of the anucleate human platelet . BMC genomics. 14.

BOOK CHAPTER

Edelstein, L.C., Bray, P.F.(2017). Noncoding RNAs in platelet biology . Platelets in Thrombotic and Non-Thrombotic Disorders: Pathophysiology, Pharmacology and Therapeutics: an Update. p. 239-252.

Edelstein, L.C., Nagalla, S., Bray, P.F.(2013). MicroRNAs in Platelet Production and Activation . MicroRNAs in Medicine. p. 101-116.

BOOK

Edelstein, L.C., Bray, P.F.(2012). Small RNAs as potential platelet therapeutics . Handbook of Experimental Pharmacology. 210. p. 435-445.