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Hire Dr. KWANGCHUL K.

United States

USD 50 /hr

Plant/algal biotechnologist producing biopharmaceuticals including vaccines and enzymes for clinical or industrial uses

Profile Summary

Expert in plant/algal-based biotechnological applications using chloroplasts as a production platform to produce therapeutics (peptides, enzymes, hormones) and vaccines. Proficient in the transformation of plant/algae (nucleus and chloroplasts), codon-optimization, cloning, vector design, tissue culture, DNA/RNA/Protein extractions and running, quantification (densitometry and ELISA), chloroplast-related experiments including isolation of chloroplast, and ribosome profiling assay. I enjoy analyzing data and writing, having published 19 peer-reviewed scientific articles including one book chapter and three review papers. Also, I am very competent in experiment designs in getting projects to move forward. In addition, I have data analytic skills using Python, R, Excel, and visualization skills using Excel dashboard and Power BI Desktop.

Subject Matter Expertise

• ☆ Molecular Biology
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Recombinant DNA
• ☆ DNA/Gene Synthesis
• ☆ Plasmid DNA
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Synthetic Biology
• ☆ Agricultural Biotechnology

Services

• Writing
• Research
• Consulting
• Data & AI

Writing Technical Writing

Research Market Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization, Big Data Analytics, Data Processing

Work Experience

CSO

Microsynbiotix

January 2017 - January 2020

Postdoc

University of Pennsylvania

April 2013 - November 2016

Postdoctor

University of Central Florida (UCF)

June 2009 - March 2013

Education

Ph.D

Yonsei University

January 1998 - January 2006

Certifications

• Data Science and Machine Learning Bootcamp with R

  udemy

  July 2022 - Present

• Python Essential and Advanced for Programming

  San Diego Continuing Education

  January 2021 - Present

Publications

JOURNAL ARTICLE

A Rom Park, Seon Woong Kim, Soon Young Kim, Kwang-Chul Kwon(2021). Expression of Antimicrobial Peptide (AMP), Cecropin B, in a Fused Form to SUMO Tag With or Without Three-Glycine Linker in Escherichia coli and Evaluation of Bacteriolytic Activity of the Purified AMP . Probiotics and Antimicrobial Proteins. 13. (6). p. 1780--1789. Springer Science and Business Media {LLC}

Da Sol Kim, Seon Woong Kim, Jae Min Song, Soon Young Kim, Kwang-Chul Kwon(2019). A new prokaryotic expression vector for the expression of antimicrobial peptide abaecin using SUMO fusion tag . BMC Biotechnology. 19. (1). Springer Science and Business Media {LLC}

(2019). An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP) . Fish & Shellfish Immunology.

(2018). Expression and assembly of largest foreign protein in chloroplasts: oral delivery of human FVIII made in lettuce chloroplasts robustly suppresses inhibitor formation in haemophilia A mice . Plant Biotechnology Journal.

(2017). Plant-based vaccines for oral delivery of type 1 diabetes-related autoantigens: Evaluating oral tolerance mechanisms and disease prevention in NOD mice . Scientific Reports.

Kwang-Chul Kwon, Hui-Ting Chan, Ileana R. León, Rosalind Williams-Carrier, Alice Barkan, Henry Daniell(2016). Codon Optimization to Enhance Expression Yields Insights into Chloroplast Translation . Plant Physiology. 172. (1). p. 62--77. American Society of Plant Biologists ({ASPB})

(2016). Oral Delivery of Protein Drugs Bioencapsulated in Plant Cells . Molecular Therapy.

(2016). Low cost delivery of proteins bioencapsulated in plant cells to human non-immune or immune modulatory cells . Biomaterials.

(2015). Low-cost oral delivery of protein drugs bioencapsulated in plant cells . Plant Biotechnology Journal.

(2014). Oral Delivery of Angiotensin-Converting Enzyme 2 and Angiotensin-(1-7) Bioencapsulated in Plant Cells Attenuates Pulmonary Hypertension . Hypertension.

(2014). Oral Delivery of ACE2/Ang-(1–7) Bioencapsulated in Plant Cells Protects against Experimental Uveitis and Autoimmune Uveoretinitis . Molecular Therapy.

(2013). Seedling Lethal1, a Pentatricopeptide Repeat Protein Lacking an E/E+ or DYW Domain in Arabidopsis, Is Involved in Plastid Gene Expression and Early Chloroplast Development . PLANT PHYSIOLOGY.

(2013). Release of Proteins from Intact Chloroplasts Induced by Reactive Oxygen Species during Biotic and Abiotic Stress . PLoS ONE.

(2013). Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells . Advanced Drug Delivery Reviews.

(2013). Oral delivery of bioencapsulated exendin-4 expressed in chloroplasts lowers blood glucose level in mice and stimulates insulin secretion in beta-TC6 cells . Plant Biotechnology Journal.

(2008). Deletion of the chloroplast-localizedAtTerCgene product inArabidopsis thalianaleads to loss of the thylakoid membrane and to seedling lethality . The Plant Journal.