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Hire Dr. Krithika R.

United States

USD 125 /hr

PhD Molecular Biologist | Assay Development, Construct Design & Biotech R&D Support

Profile Summary

Ph.D.-trained molecular biologist with over 10 years of experience spanning translational academic research and industry R&D in biotechnology. I specialize in experimental design, molecular cloning, mammalian cell culture, and assay development to support early discovery and platform development programs. My background includes designing DNA constructs, supporting antibody expression workflows, and developing application-focused experiments for nucleic acid and biologics platforms. In industry, I’ve led technical projects supporting pharmaceutical collaborations and worked closely with cross-functional teams across R&D, manufacturing, and product development to deliver reproducible, well-documented results. Earlier in my career, I conducted translational cancer research focused on biomarker biology, protein function, and post-translational modifications. I bring a strong bench-science foundation paired with a practical, results-driven mindset shaped by product-oriented environments. I offer consulting support in areas including molecular biology workflows, assay strategy and troubleshooting, construct design, experimental planning, data interpretation, and scientific documentation. I enjoy collaborating with teams to solve complex problems, improve experimental rigor, and accelerate research outcomes. I’m available for short-term consulting, experimental design support, technical reviews, and scientific writing projects.

Subject Matter Expertise

• ★ Microbiology
• ★ Biochemistry
• ★ DNA/Gene Synthesis
• ☆ Microbiological Assays
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Structural Biology
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ Biomarkers
• ☆ Genetics
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Next Generation Sequencing (NGS)
• ☆ DNA Sequencing
• ☆ Synthetic Genomics
• ☆ Genome Sequencing
• ☆ Mutagenesis
• ☆ Recombinant DNA
• ☆ RNA Synthesis
• ☆ Oligonucleotide Synthesis
• ☆ Plasmid DNA
• ☆ Kinases
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cell Signaling Pathways
• ☆ Gene Regulation
• ☆ Pathogenesis
• ☆ Biomarker Discovery
• ☆ Cell Culture
• ☆ Blotting
• ☆ Immunohistochemistry
• ☆ Technical & Scientific Writing

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing, General Proofreading & Editing

Research Fact Checking, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Data Visualization

Product Development Formulation, Recipe Development, Product Evaluation, Product Validation, Manufacturing, Product Launch Support

Work Experience

Consultant

Self Employed

January 2026 - Present

Scientist

Telesis Bio Inc

January 2022 - January 2026

Postdoctoral fellow

University of California San Diego

November 2018 - January 2022

Research technician

Johns Hopkins University

June 2011 - June 2012

Education

Doctor of Philosophy (Biological Sciences)

Columbia University

September 2012 - October 2018

MA

Columbia University

August 2012 - July 2014

Masters (Biotechnology)

Johns Hopkins University

September 2009 - May 2011

Bachelor of pharmacy (Pharmacy)

Savitribai Phule Pune University

August 2004 - June 2008

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2020). Escherichia coli YegI is a novel Ser/Thr kinase lacking conserved motifs that localizes to the inner membrane . FEBS letters.

Rajagopalan, Krithika, Dworkin, Jonathan (2020). Escherichia coliYegI is a novel Ser/Thr kinase lacking conserved motifs that localizes to the inner membrane . FEBS Letters.

(2019). Structural and Dynamical Order of a Disordered Protein: Molecular Insights into Conformational Switching of PAGE4 at the Systems Level . Biomolecules.

Rajagopalan, Krithika, Lin, Xingcheng, Kulkarni, Prakash, Bocci, Federico, Schafer, Nicholas P., Roy, Susmita, Tsai, Min-Yeh, He, Yanan, Chen, Yihong, Mooney, Steven M., et al. (2019). Structural and Dynamical Order of a Disordered Protein: Molecular Insights into Conformational Switching of PAGE4 at the Systems Level . Biomolecules.

(2018). Identification and Biochemical Characterization of a Novel Protein Phosphatase 2C-Like Ser/Thr Phosphatase in Escherichia coli . Journal of bacteriology.

Krithika Rajagopalan, Jonathan Dworkin, Ann M. Stock(2018). Identification and Biochemical Characterization of a Novel Protein Phosphatase 2C-Like Ser/Thr Phosphatase in Escherichia coli . Journal of Bacteriology. 200. (18). American Society for Microbiology

Rajagopalan, Krithika, Dworkin, Jonathan (2018). Identification and Biochemical Characterization of a Novel Protein Phosphatase 2C-Like Ser/Thr Phosphatase in Escherichia coli . Journal of Bacteriology.

Krithika Rajagopalan, Govindan Rangarajan, Prakash Kulkarni, StevenM Mooney(2016). Cancer/testis antigens and obligate participation in multiple hallmarks of cancer: an update . Asian Journal of Andrology. 18. (5). p. 711. Medknow

Rajagopalan, Krithika, Mooney, Steven M., Rangarajan, Govindan, Kulkarni, Prakash (2016). Cancer/testis antigens and obligate participation in multiple hallmarks of cancer: an update . Asian Journal of Andrology.

Krithika Rajagopalan, Yanan He, Yihong Chen, Steven M. Mooney, Ajay Bhargava, Elizabeth Sacho, Keith Weninger, Philip N. Bryan, Prakash Kulkarni, John Orban(2015). Phosphorylation-induced Conformational Ensemble Switching in an Intrinsically Disordered Cancer/Testis Antigen . Journal of Biological Chemistry. 290. (41). p. 25090--25102. American Society for Biochemistry {\&} Molecular Biology ({ASBMB})

Rajagopalan, Krithika, He, Yanan, Chen, Yihong, Mooney, Steven M., Bhargava, Ajay, Sacho, Elizabeth, Weninger, Keith, Bryan, Philip N., Kulkarni, Prakash, Orban, John (2015). Phosphorylation-induced Conformational Ensemble Switching in an Intrinsically Disordered Cancer/Testis Antigen . Journal of Biological Chemistry.

(2014). Structure of the Arabidopsis thaliana TOP2 oligopeptidase . Acta crystallographica. Section F, Structural biology communications.

Krithika Rajagopalan, Steven M. Mooney, Ruoyi Qiu, John J. Kim, Elizabeth J. Sacho, Dorhyun Johng, Takumi Shiraishi, Prakash Kulkarni, Keith R. Weninger(2014). Cancer/Testis Antigen PAGE4, a Regulator of c-Jun Transactivation, Is Phosphorylated by Homeodomain-Interacting Protein Kinase 1, a Component of the Stress-Response Pathway . Biochemistry. 53. (10). p. 1670--1679. American Chemical Society ({ACS})

Krithika Rajagopalan, Ruoyi Qiu, Steven M. Mooney, Shweta Rao, Takumi Shiraishi, Elizabeth Sacho, Hongying Huang, Ellen Shapiro, Keith R. Weninger, Prakash Kulkarni(2014). The Stress-response protein prostate-associated gene 4, interacts with c-Jun and potentiates its transactivation . Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1842. (2). p. 154--163. Elsevier {BV}

Rajagopalan, Krithika, Qiu, Ruoyi, Mooney, Steven M., Rao, Shweta, Shiraishi, Takumi, Sacho, Elizabeth, Huang, Hongying, Shapiro, Ellen, Weninger, Keith R., Kulkarni, Prakash (2014). The Stress-response protein prostate-associated gene 4, interacts with c-Jun and potentiates its transactivation . Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease.

Rajagopalan, Krithika, Mooney, Steven M., Qiu, Ruoyi, Kim, John J., Sacho, Elizabeth J., Johng, Dorhyun, Shiraishi, Takumi, Kulkarni, Prakash, Weninger, Keith R. (2014). Cancer/Testis Antigen PAGE4, a Regulator of c-Jun Transactivation, Is Phosphorylated by Homeodomain-Interacting Protein Kinase 1, a Component of the Stress-Response Pathway . Biochemistry.

Rajagopalan, Krithika, Wang, Ruiying, Sadre-Bazzaz, Kianoush, Moreau, Magali, Klessig, Daniel F., Tong, Liang (2014). Structure of the Arabidopsis thaliana TOP2 oligopeptidase . Acta Crystallographica Section F: Structural Biology Communications.

(2013). CETN1 is a cancer testis antigen with expression in prostate and pancreatic cancers . Biomarker research.

Krithika Rajagopalan, John J. Kim, Bo Yin, Christhunesa S. Christudass, Naoki Terada, Ben Fabry, Danielle Y. Lee, Takumi Shiraishi, Robert H. Getzenberg, Robert W. Veltri, et al.(2013). Acquisition of paclitaxel resistance is associated with a more aggressive and invasive phenotype in prostate cancer . Journal of Cellular Biochemistry. 114. (6). p. 1286--1293. Wiley

Krithika Rajagopalan, Gita Mahmoudabadi, Robert H. Getzenberg, Sridhar Hannenhalli, Govindan Rangarajan, Prakash Kulkarni(2013). Intrinsically disordered proteins and conformational noise . Cell Cycle. 12. (1). p. 26--31. Informa {UK} Limited

Rajagopalan, Krithika, Mahmoudabadi, Gita, Getzenberg, Robert H., Hannenhalli, Sridhar, Rangarajan, Govindan, Kulkarni, Prakash (2013). Intrinsically disordered proteins and conformational noise Implications in cancer . Cell Cycle.

Rajagopalan, Krithika, Kim, John J., Yin, Bo, Christudass, Christhunesa S., Terada, Naoki, Fabry, Ben, Lee, Danielle Y., Shiraishi, Takumi, Getzenberg, Robert H., Veltri, Robert W., et al. (2013). Acquisition of paclitaxel resistance is associated with a more aggressive and invasive phenotype in prostate cancer . Journal of Cellular Biochemistry.

Rajagopalan, Krithika, Kim, John J., Hussain, Basil, Williams, Brenten H., Kulkarni, Prakash, Mooney, Steven M. (2013). CETN1 is a cancer testis antigen with expression in prostate and pancreatic cancers . Biomarker Research.

(2012). Intrinsically disordered proteins and conformational noise: implications in cancer . Cell cycle (Georgetown, Tex.).

Krithika Rajagopalan, Prakash Kulkarni, Takumi Shiraishi, Robert Kim, Steven M. Mooney, Robert H. Getzenberg(2012). Cancer/testis antigens and urological malignancies . Nature Reviews Urology. 9. (7). p. 386--396. Springer Nature

Rajagopalan, Krithika, Kulkarni, Prakash, Shiraishi, Takumi, Kim, Robert, Mooney, Steven M., Getzenberg, Robert H. (2012). Cancer/testis antigens and urological malignancies . Nature Reviews Urology.

Krithika Rajagopalan, Steven M. Mooney, Nehal Parekh, Robert H. Getzenberg, Prakash Kulkarni(2011). A majority of the cancer/testis antigens are intrinsically disordered proteins . Journal of Cellular Biochemistry. 112. (11). p. 3256--3267. Wiley

Krithika Rajagopalan, Prakash Kulkarni, David Yeater, Robert H. Getzenberg(2011). Protein folding and the order/disorder paradox . Journal of Cellular Biochemistry. 112. (7). p. 1949--1952. Wiley

Krithika Rajagopalan, Steven M. Mooney, Brenten H. Williams, Yu Zeng, Christhunesa S. Christudass, Youqiang Li, Bo Yin, Prakash Kulkarni, Robert H. Getzenberg(2011). Creatine kinase brain overexpression protects colorectal cells from various metabolic and non-metabolic stresses . Journal of Cellular Biochemistry. 112. (4). p. 1066--1075. Wiley

Rajagopalan, Krithika, Mooney, Steven M., Williams, Brenten H., Zeng, Yu, Christudass, Christhunesa S., Li, Youqiang, Yin, Bo, Kulkarni, Prakash, Getzenberg, Robert H. (2011). Creatine Kinase Brain Overexpression Protects Colorectal Cells From Various Metabolic and Non-Metabolic Stresses . Journal of Cellular Biochemistry.

Rajagopalan, Krithika, Kulkarni, Prakash, Yeater, David, Getzenberg, Robert H. (2011). Protein Folding and the Order/Disorder Paradox . Journal of Cellular Biochemistry.

Rajagopalan, Krithika, Mooney, Steven M., Parekh, Nehal, Getzenberg, Robert H., Kulkarni, Prakash (2011). A Majority of the Cancer/Testis Antigens Are Intrinsically Disordered Proteins . Journal of Cellular Biochemistry.

OTHER

Krithika Rajagopalan, Jonathan Dworkin(2018). Identification and biochemical characterization of a novel PP2C-like Ser/Thr phosphatase inE. coli . Cold Spring Harbor Laboratory