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USD 400 /hr
Hire Dr. John L.
United States
USD 400 /hr

Molecular life scientist with decades of experience in successfully executing experimental R&D projects

Profile Summary
Subject Matter Expertise
Services
Work Experience

Principal Investigator, Associate Professor

University Medical Center Groningen

July 2019 - Present

Research Associate Professor

Rockefeller University

June 2019 - Present

Education

PhD Molecular Genetics

University of Edinburgh

September 2000 - October 2005

Certifications
  • Certification details not provided.
Publications
JOURNAL ARTICLE
(2020). Line-1 Orf2p Expression is Nearly Imperceptible in Human Cancers . Mobile Dna.
(2020). Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes . Methods in Molecular Biology.
John LaCava, Yuhui Dou, Isabelle Barbosa, Hua Jiang, Claudia Iasillo, Kelly R Molloy, Wiebke Manuela Schulze, Stephen Cusack, Manfred Schmid, Hervé Le Hir, et al.(2020). {NCBP}3 positively impacts {mRNA} biogenesis . Nucleic Acids Research. 48. (18). p. 10413--10427. Oxford University Press ({OUP})
(2020). Affinity Proteomic Dissection of the Human Nuclear Cap-binding Complex Interactome . Nucleic Acids Research.
(2020). Ncbp3 Positively Impacts Mrna Biogenesis . Nucleic Acids Research.
(2020). Nci Think-tank Meeting Report on Proteomic Cartography and Biomarkers at the Single-cell Level: Interrogation of Premalignant Lesions . Journal of Proteome Research.
(2019). To the Editor in Reply to Dr. Crow's Letter . Arthritis & Rheumatology.
(2019). High Prevalence and Disease Correlation of Autoantibodies Against P40 Encoded by Long Interspersed Nuclear Elements (line-1) in Systemic Lupus Erythematosus . Arthritis & Rheumatology.
(2019). Identification of Rnase-sensitive Line-1 Ribonucleoprotein Interactions by Differential Affinity Immobilization . Bio-protocol.
John LaCava, Tomas Mustelin, Kennedy Ukadike, Marty Taylor(2019). Reply . Arthritis {\&} Rheumatology. 72. (2). p. 376--377. Wiley
John LaCava(2019). {RNA} Binding Proteins as Regulators of Retrotransposon-Induced Exonization . {BioEssays}. p. 1800263. Wiley
(2019). Rna Binding Proteins As Regulators of Retrotransposon-induced Exonization . Bioessays.
John LaCava, Victoria Carter, Martin S. Taylor, Shu Ying Liang, Cecilia Mustelin, Kennedy C. Ukadike, Anders Bengtsson, Christian Lood, Tomas Mustelin(2019). High Prevalence and Disease Correlation of Autoantibodies Against p40 Encoded by Long Interspersed Nuclear Elements in Systemic Lupus Erythematosus . Arthritis {\&} Rheumatology. 72. (1). p. 89--99. Wiley
John LaCava, Elodie Pronier, Paolo Cifani, Tiffany R. Merlinsky, Katharine Barr Berman, Amritha Varshini Hanasoge Somasundara, Raajit K. Rampal, Karen E. Wei, Friederike Pastore, Jesper L.V. Maag, et al. (2018). Targeting the CALR interactome in myeloproliferative neoplasms . JCI Insight.
(2018). Targeting the Calr Interactome in Myeloproliferative Neoplasms . Jci Insight.
(2018). Functions of Unconventional Mammalian Translational Gtpases Gtpbp1 and Gtpbp2 . Genes & Development.
(2018). Polycomb Complexes Associate with Enhancers and Promote Oncogenic Transcriptional Programs in Cancer Through Multiple Mechanisms . Nature Communications.
John LaCava, Paolo Mita, Aleksandra Wudzinska, Xiaoji Sun, Joshua Andrade, Shruti Nayak, David J Kahler, Sana Badri, Beatrix Ueberheide, Chi Y Yun, et al.(2018). {LINE}-1 protein localization and functional dynamics during the cell cycle . {eLife}. 7. {eLife} Sciences Organisation, Ltd.
Winczura K, Schmid M, Iasillo C, Molloy KR, Harder LM, Andersen JS, LaCava J, Jensen TH(2018). Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions . Cell reports.
(2018). Line-1 Protein Localization and Functional Dynamics During the Cell Cycle . Elife.
(2018). Characterizing Zc3h18, a Multi-domain Protein at the Interface of Rna Production and Destruction Decisions . Cell Reports.
Taylor MS, Altukhov I, Molloy KR, Mita P, Jiang H, Adney EM, Wudzinska A, Badri S, Ischenko D, Eng G, et al.(2018). Dissection of affinity captured LINE-1 macromolecular complexes . eLife.
(2018). Dissection of Affinity Captured Line-1 Macromolecular Complexes . Elife.
John LaCava, Yinxing Ma, Licia Silveri, Svetlana Dokudovskaya(2017). Tumor suppressor {NPRL}2 induces {ROS} production and {DNA} damage response . Scientific Reports. 7. (1). Springer Nature
John LaCava, Alexandra Santanach, Enrique Blanco, Hua Jiang, Kelly R. Molloy, Miriam Sansó, Lluis Morey, Luciano Di Croce(2017). The Polycomb group protein {CBX}6 is an essential regulator of embryonic stem cell identity . Nature Communications. 8. (1). Springer Nature
(2017). The Polycomb Group Protein Cbx6 is an Essential Regulator of Embryonic Stem Cell Identity . Nature Communications.
(2017). The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity . Nature communications.
(2017). Tumor suppressor NPRL2 induces ROS production and DNA damage response . Scientific reports.
(2017). Tumor Suppressor Nprl2 Induces Ros Production and Dna Damage Response . Scientific Reports.
Domanski M, LaCava J, Bio-protocol, 2017, vol. 7, no. 8, 2017 .
(2017). Rna Degradation Assay Using Rna Exosome Complexes, Affinity-purified from Hek-293 Cells . Bio-protocol.
John LaCava, Michal Domanski(2017). Affinity Purification of the {RNA} Degradation Complex, the Exosome, from {HEK}-293 Cells . {BIO}-{PROTOCOL}. 7. (8). Bio-Protocol, {LLC}
John LaCava, Michal Domanski(2017). {RNA} Degradation Assay Using {RNA} Exosome Complexes, Affinity-purified from {HEK}-293 Cells . {BIO}-{PROTOCOL}. 7. (8). Bio-Protocol, {LLC}
(2016). Protein Complex Affinity Capture from Cryomilled Mammalian Cells . Journal of visualized experiments : JoVE.
John LaCava, Michal Domanski, Paula Upla, William J. Rice, Kelly R. Molloy, Natalia E. Ketaren, David L. Stokes, Torben Heick Jensen, Michael P. Rout(2016). Purification and analysis of endogenous human {RNA} exosome complexes . {RNA}. 22. (9). p. 1467--1475. Cold Spring Harbor Laboratory
(2016). Purification and analysis of endogenous human RNA exosome complexes . RNA.
John LaCava, Javier Fernandez-Martinez, Michael P. Rout(2016). Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture . Cold Spring Harbor Protocols. 2016. (7). p. pdb.prot087957. Cold Spring Harbor Laboratory
(2016). Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes After Affinity Capture . Csh Protocols.
(2016). Protein Complex Purification by Affinity Capture . Csh Protocols.
(2016). Optimized Affinity Capture of Yeast Protein Complexes . Csh Protocols.
John LaCava, Javier Fernandez-Martinez, Zhanna Hakhverdyan, Michael P. Rout(2016). Optimized Affinity Capture of Yeast Protein Complexes . Cold Spring Harbor Protocols. 2016. (7). p. pdb.prot087932. Cold Spring Harbor Laboratory
(2016). Native Elution of Yeast Protein Complexes Obtained by Affinity Capture . Csh Protocols.
John LaCava, Javier Fernandez-Martinez, Michael P. Rout(2016). Native Elution of Yeast Protein Complexes Obtained by Affinity Capture . Cold Spring Harbor Protocols. 2016. (7). p. pdb.prot087940. Cold Spring Harbor Laboratory
John LaCava, Javier Fernandez-Martinez, Zhanna Hakhverdyan, Michael P. Rout(2016). Protein Complex Purification by Affinity Capture . Cold Spring Harbor Protocols. 2016. (7). p. pdb.top077545. Cold Spring Harbor Laboratory
LaCava J, Fernandez-Martinez J, Hakhverdyan Z, Rout MP, Cold Spring Harbor protocols, 2016, vol. 2016, no. 7, pp. pdb.top077545 .
(2016). Characterization of L1-Ribonucleoprotein Particles . Methods in molecular biology.
Fernandez-Martinez J, LaCava J, Rout MP, Cold Spring Harbor protocols, 2016, vol. 2016, no. 7, pp. pdb.prot087957 .
LaCava J, Fernandez-Martinez J, Hakhverdyan Z, Rout MP, Cold Spring Harbor protocols, 2016, vol. 2016, no. 7, pp. pdb.prot087932 .
LaCava J, Fernandez-Martinez J, Rout MP, Cold Spring Harbor protocols, 2016, vol. 2016, no. 7, pp. pdb.prot087940 .
(2015). Interactomic and Enzymatic Analyses of Distinct Affinity Isolated Human Retrotransposon Intermediates. Protein science.
(2015). Rapid, optimized interactomic screening . Nature methods.
Hakhverdyan Z, Domanski M, Hough LE, Oroskar AA, Oroskar AR, Keegan S, Dilworth DJ, Molloy KR, Sherman V, Aitchison JD, Fenyö D, Chait BT, Jensen TH, Rout MP, LaCava J, Nature methods, 2015 .
(2015). Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives . Biotechniques.
LaCava J, Molloy KR, Taylor MS, Domanski M, Chait BT, Rout MP, BioTechniques, 2015, vol. 58, no. 3, pp. 103-119 .
John LaCava, Lixin Dai, Martin S Taylor, Jef D Boeke(2014). Expression and detection of {LINE}-1 {ORF}-encoded proteins . Mobile Genetic Elements. 4. (3). p. e29319. Informa {UK} Limited
(2014). Expression and detection of LINE-1 ORF-encoded proteins . Mobile genetic elements.
(2013). The human cap-binding complex is functionally connected to the nuclear RNA exosome . Nature structural & molecular biology.
(2013). Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition . Cell.
(2013). Improved native isolation of endogenous Protein A-tagged protein complexes . Biotechniques.
LaCava, John, Chandramouli, Nagarajan, Jiang, Hua, Rout, Michael P.(2013). Improved Native Isolation of Endogenous Protein A-Tagged Protein Complexes . Biotechniques. 54. (4). p. 213-216.
Andersen, P.R., Domanski, M., Kristiansen, M.S., Storvall, H., Ntini, E., Verheggen, C., Schein, A., Bunkenborg, J., Poser, I., Hallais, M., et al.(2013). The human cap-binding complex is functionally connected to the nuclear RNA exosome . Nature Structural and Molecular Biology. 20. (12). p. 1367-1376.
Taylor, M.S., Lacava, J., Mita, P., Molloy, K.R., Huang, C.R.L., Li, D., Adney, E.M., Jiang, H., Burns, K.H., Chait, B.T., et al.(2013). Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition . Cell. 155. (5). p. 1034-1048.
(2012). Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels . Biotechniques.
(2012). High-Throughput, Single-Step Purification of Affinity-Tagged Protein Complexes. FASEB journal.
LaCava, John, Chait, Brian T, Domanski, Michal, Jensen, Torben Heick, Jiang, Hua, Molloy, Kelly, Rout, Michael P(2012). Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels . BioTechniques. 0. (0). p. 1-6.
LaCava, John, Chait, Brian T., Domanski, Michal, Fenyo, David, Hakhverdyan, Zhanna, Hough, Loren, Jensen, Torben Heick, Oroskar, Anil, Oroskar, Asha, Rout, Michael P.(2010). High-Throughput, Single-Step Purification of Affinity-Tagged Protein Complexes. FASEB Journal. 24.
(2006). RNA-quality control by the exosome . Nature reviews. Molecular cell biology.
LaCava, John, Houseley, Jonathan, Tollervey, David(2006). RNA-quality control by the exosome . Nature Reviews Molecular Cell Biology. 7. (7). p. 529-539.
(2005). RNA degradation by the exosome is promoted by a nuclear polyadenylation complex . Cell.
Houseley, J, Jacquier, A, LaCava, J, Petfalski, E, Saveanu, C, Thompson, E, Tollervey, D(2005). RNA degradation by the exosome is promoted by a nuclear polyade nylation complex . Cell. 121. (5). p. 713-724.
OTHER
John LaCava, Yuhui Dou, Isabelle Barbosa, Hua Jiang, Claudia Iasillo, Kelly R. Molloy, Wiebke Manuela Schulze, Stephen Cusack, Manfred Schmid, Hervé Le Hir, et al.(2020). {NCBP}3 is a productive {mRNP} component . Cold Spring Harbor Laboratory
John LaCava, Mehrnoosh Oghbaie, Petr Ć ulc, David Fenyö, Michael Pennock(2019). Complex$\mathplus$: Aided Decision-Making for the Study of Protein Complexes . Cold Spring Harbor Laboratory