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Contact John B.

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Profile Details

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USD 100 /hr

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Hire Dr. John B.

United States

USD 100 /hr

Aptamer, Food Safety/Microbiology/SBIR and Grant Writing Expert

Profile Summary

Career Summary/Highlights:
- Spearheaded acquisition/licensing of DNA Aptamer technology to ApolloDx and CibusDx.
- Led team at Pronucleotein, Inc. which won 2nd Place (49 competitors) in the 2014 FDA Food Safety Testing Tech Challenge ($120K in total prize money;http://www.foodsafetychallenge.com/).
- Launched a profitable DNA Aptamer sales website www.OTCBiotech.com.
- Winner of the 2011 Small Business Administration’s Tibbetts Award for Excellence in SBIR R&D.
- Awarded > 80 SBIR/STTR, other federal and state R & D contracts totaling > $20M.
- Published 110 total peer-reviewed journal articles, 7 book chaps. & inventor on 16 issued US & EU patents.
- Edited & contributed 3 chapters to a book entitled "Biomedical Applications of Aptamers," Nova Science Publishers (formerly Plenum Press).
- Patented a method for producing intrachain FRET-aptamers and assays
- Patented and published a facile 3’ attachment chemistry to couple PCR products to proteins for low-cost industrial-scale mass production of DNA-3’-protein conjugates which are stable in vivo.
- Invented and published DNA Aptamer-Complement conjugates to kill antibiotic-resistant infections (3 Patents Issued on Technology).
- Invented and published DNA Aptamer-Fc opsonins to combat encapsulated pathogenic bacteria.
- Developed & published DNA aptamer-based colloidal gold and fluorescent (nanoparticle and quantum dot-based) lateral flow test strips for the US Army to detect key hemorrhagic and other pathogenic viruses (e.g., Chikungunya, Crimean-Congo, Dengue, and West Nile viruses).
- Developed an aptamer beacon assay for C-telopeptide (CTx) to monitor astronaut bone loss for NASA. Also developed N-acetylglucosamine and chitin aptamers to monitor recycled water aboard spacecraft for NASA under Phase 1 and Phase 2 SBIR contracts.
- Invented and published novel FRET-Aptamer methodology and assays for bone markers, calcidiol, and organophosphorus nerve agent detection and quantification.

Subject Matter Expertise

Services

Writing
Research
Consulting
Data & AI
Product Development

Writing Technical Writing, Copywriting, Newswriting

Research Market Research, User Research, Meta-Research, Gap Analysis, Gray Literature Search, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis

Product Development Product Evaluation, Concept Development

Work Experience

Director of Biotechnology

Nanohmics Inc

September 2018 - Present

Chief Science Officer

OTC Biotech/Pronucleotein

January 2003 - August 2018

Education

Doctorate

University of Arizona

August 1986 - May 1991

Certifications

Drug Development (including clinical trials and FDA approval)

U.C. SanDiego

November 2019 - Present

Publications

JOURNAL ARTICLE

Jeremy J. John, Christopher J. May, John G. Bruno(2021). A Combined Immunofluorescence and Fluorescent Viability Cocktail Staining Procedure for Rapid Microscopic Detection and Enumeration of Live Legionella pneumophila . Journal of Fluorescence. Springer Science and Business Media {LLC}

John G. Bruno(2019). Integration of multiple computer modeling software programs for characterization of a brain natriuretic peptide sandwich DNA aptamer complex . Journal of Molecular Recognition. 32. (12). Wiley