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USD 70 /hr
Hire Jani A.
Portugal
USD 70 /hr

Translational Immunology Researcher | Flow Cytometry & Scientific Writing Consultant

Profile Summary
Subject Matter Expertise
Services
Writing Clinical Trial Documentation, Technical Writing
Research Scientific and Technical Research
Consulting Scientific and Technical Consulting
Work Experience

Invited Assistant Professor

University of Coimbra

September 2025 - Present

Researcher - Immunology/Flow Cytometry

University of Coimbra

July 2025 - Present

Invited Assistant

University of Coimbra

September 2022 - August 2025

Flow Cytometry Research Fellow, Research Laboratory Coordinator and Web Platform Manager at the Institute of Immunology

University of Coimbra

July 2024 - July 2025

PhD Scholarship (FCT)

University of Coimbra

January 2020 - February 2024

Research Assistant at the Laboratory of immunology and Oncology

University of Coimbra

October 2014 - December 2019

Education

Doctor of Philosofy - Health Sciences, Biomedical Sciences (Faculty of Medicine)

University of Coimbra

September 2019 - July 2024

Master of Science - Biochemistry (Faculty of Sciences and Technology)

University of Coimbra

September 2012 - September 2014

Bachelor of Science - Biochemistry (Faculty of Sciences and Technology)

University of Coimbra

September 2008 - July 2013

Certifications

  • Programa de Doutoramento em Ciências da Saúde - Ciências Biomédicas at the Faculdade de Medicina of the Universidade de Coimbra

    University of Coimbra

    January 2026 - Present

  • Master’s Degree in Biochemistry

    University of Coimbra

    January 2026 - Present

  • Bachelor degree in Biochemistry

    University of Coimbra

    January 2026 - Present

Publications
JOURNAL ARTICLE
Nuno Carvalho, Jani-Sofia Almeida, Elisabete Carolino, Francisco Lopes, Susana Henriques, João Vaz, Hélder Coelho, Paulo Rodrigues dos Santos, Manuel Santos Rosa, Luís Moita, et al. (2026). Characterization of Th2 Serum Immune Response in Acute Appendicitis . International Journal of Molecular Sciences.
Characterization of Th2 Serum Immune Response in Acute Appendicitis @article { author = {Nuno Carvalho and Jani-Sofia Almeida and Elisabete Carolino and Francisco Lopes and Susana Henriques and João Vaz and Hélder Coelho and Paulo Rodrigues dos Santos and Manuel Santos Rosa and Luís Moita and Carlos Luz and Paulo Matos da Costa}, title = {Characterization of Th2 Serum Immune Response in Acute Appendicitis}, journal = {International Journal of Molecular Sciences}, year = {2026}, month = {jan}, url = {https://doi.org/10.3390/ijms27020733}, doi = {10.3390/ijms27020733} } . International Journal of Molecular Sciences.
Nuno Carvalho, Jani-Sofia Almeida, Elisabete Carolino, Francisco Lopes, Susana Henriques, João Vaz, Hélder Coelho, Paulo Rodrigues dos Santos, Manuel Santos Rosa, Luís Moita, et al. (2026). Characterization of Th2 Serum Immune Response in Acute Appendicitis . International Journal of Molecular Sciences.
Intestinal mucosal alterations parallel central demyelination and remyelination: insights into the gut-brain axis in the cuprizone model of multiple sclerosis @article { author = {Ferreira, Carolina and Carvalho, Filipa and Vieira, Pedro and Alves, André and Palavra, Filipe and Almeida, Jani and Alves, Vera and et al.}, title = {Intestinal mucosal alterations parallel central demyelination and remyelination: insights into the gut-brain axis in the cuprizone model of multiple sclerosis}, journal = {Frontiers in Immunology}, volume = {16}, year = {2025}, month = {oct}, url = {https://doi.org/10.3389/fimmu.2025.1682183}, doi = {10.3389/fimmu.2025.1682183} } . Frontiers in Immunology.
AI-assisted peripheral immune profiling reveals unconventional lymphocyte signatures associated with prognosis in soft tissue sarcoma patients @article { author = {Almeida, Jani Sofia and Sousa, Luana Madalena and Couceiro, Patrícia and Alves, Vera and Rodrigues, Joana and Fonseca, Ruben and Freitas-Tavares, Paulo and et al.}, title = {AI-assisted peripheral immune profiling reveals unconventional lymphocyte signatures associated with prognosis in soft tissue sarcoma patients}, journal = {Frontiers in Immunology}, volume = {16}, year = {2025}, month = {oct}, url = {https://doi.org/10.3389/fimmu.2025.1677408}, doi = {10.3389/fimmu.2025.1677408} } . Frontiers in Immunology.
Modulation of Immune Cells by Aspirin During Pregnancy @article { author = {Areia, Ana Luísa and Almeida, Jani and Alves, Vera and Mota-Pinto, Anabela and Sá, Helena}, title = {Modulation of Immune Cells by Aspirin During Pregnancy}, journal = {Immunology}, year = {2025}, month = {oct}, url = {https://doi.org/10.1111/imm.70051}, doi = {10.1111/imm.70051} } . Immunology.
Persistent Proinflammatory Cytokine Profile in the Tear Fluid of Stable Keratoconus: Rethinking Clinical Quiescence @article { author = {Gil, Pedro and Gil, João Quadrado and Cruz, Nuno and Costa, Celso and Rodrigues-Santos, Paulo and Sousa, Luana Madalena and Almeida, Jani Sofia and et al.}, title = {Persistent Proinflammatory Cytokine Profile in the Tear Fluid of Stable Keratoconus: Rethinking Clinical Quiescence}, journal = {Translational Vision Science & Technology}, volume = {14}, number = {9}, pages = {31}, year = {2025}, month = {sep}, url = {https://doi.org/10.1167/tvst.14.9.31}, doi = {10.1167/tvst.14.9.31} } . Translational Vision Science & Technology.
Editorial: Immunonutrition: bridging precision nutrition and modern medicine @article { author = {Almeida, Jani and Sá, Helena and Pérez-Cano, Francisco José and Viana, Sofia}, title = {Editorial: Immunonutrition: bridging precision nutrition and modern medicine}, journal = {Frontiers in Nutrition}, volume = {12}, year = {2025}, month = {aug}, url = {https://doi.org/10.3389/fnut.2025.1685397}, doi = {10.3389/fnut.2025.1685397} } . Frontiers in Nutrition.
Comprehensive Receptor Repertoire and Functional Analysis of Peripheral NK Cells in Soft Tissue Sarcoma Patients @article { author = {Luana Madalena Sousa and Jani-Sofia Almeida and Tânia Fortes-Andrade and Patrícia Couceiro and Joana Rodrigues and Rúben Fonseca and Manuel Santos-Rosa and Paulo Freitas-Tavares and José Manuel Casanova and Paulo Rodrigues-Santos}, title = {Comprehensive Receptor Repertoire and Functional Analysis of Peripheral NK Cells in Soft Tissue Sarcoma Patients}, journal = {Cancers}, year = {2025}, month = {jul}, url = {https://doi.org/10.3390/cancers17152508}, doi = {10.3390/cancers17152508} } . Cancers.
Luana Madalena Sousa, Jani-Sofia Almeida, Tânia Fortes-Andrade, Patrícia Couceiro, Joana Rodrigues, Rúben Fonseca, Manuel Santos-Rosa, Paulo Freitas-Tavares, José Manuel Casanova, Paulo Rodrigues-Santos (2025). Comprehensive Receptor Repertoire and Functional Analysis of Peripheral NK Cells in Soft Tissue Sarcoma Patients . Cancers.
Luana Madalena Sousa, Jani-Sofia Almeida, Tânia Fortes-Andrade, Patrícia Couceiro, Joana Rodrigues, Rúben Fonseca, Manuel Santos-Rosa, Paulo Freitas-Tavares, José Manuel Casanova, Paulo Rodrigues-Santos (2025). Comprehensive Receptor Repertoire and Functional Analysis of Peripheral NK Cells in Soft Tissue Sarcoma Patients . Cancers.
Immune monitoring of trabectedin therapy in refractory soft tissue sarcoma patients - the IMMUNYON study @article { author = {Rodrigues-Santos, Paulo and Almeida, Jani Sofia and Sousa, Luana Madalena and Couceiro, Patrícia and Martinho, António and Rodrigues, Joana and Fonseca, Ruben and et al.}, title = {Immune monitoring of trabectedin therapy in refractory soft tissue sarcoma patients - the IMMUNYON study}, journal = {Frontiers in Immunology}, volume = {16}, year = {2025}, month = {feb}, url = {https://doi.org/10.3389/fimmu.2025.1516793}, doi = {10.3389/fimmu.2025.1516793} } . Frontiers in Immunology.
Peripheral immune profiling of soft tissue sarcoma: perspectives for disease monitoring @article { author = {Almeida, Jani Sofia and Sousa, Luana Madalena and Couceiro, Patrícia and Andrade, Tânia Fortes and Alves, Vera and Martinho, António and Rodrigues, Joana and et al.}, title = {Peripheral immune profiling of soft tissue sarcoma: perspectives for disease monitoring}, journal = {Frontiers in Immunology}, volume = {15}, year = {2024}, month = {oct}, url = {https://doi.org/10.3389/fimmu.2024.1391840}, doi = {10.3389/fimmu.2024.1391840} } . Frontiers in Immunology.
Connexin43 promotes exocytosis of damaged lysosomes through actin remodelling @article { author = {Neuza Domingues and Steve Catarino and Beatriz Cristóvão and Lisa Rodrigues and Filomena A Carvalho and Maria João Sarmento and Mónica Zuzarte and Jani Almeida and Teresa Ribeiro-Rodrigues and Ânia Correia-Rodrigues and Fábio Fernandes and Paulo Rodrigues-Santos and Trond Aasen and Nuno C Santos and Viktor I Korolchuk and Teresa Gonçalves and Ira Milosevic and Nuno Raimundo and Henrique Girão}, title = {Connexin43 promotes exocytosis of damaged lysosomes through actin remodelling}, journal = {The EMBO Journal}, year = {2024}, month = {jul}, url = {https://doi.org/10.1038/s44318-024-00177-3}, doi = {10.1038/s44318-024-00177-3} } . The EMBO Journal.
Jani Almeida, Neuza Domingues, Steve Catarino, Beatriz Cristóvão, Lisa Rodrigues, Filomena A Carvalho, Maria João Sarmento, Mónica Zuzarte, Teresa Ribeiro-Rodrigues, Ânia Correia-Rodrigues, et al. (2024). Connexin43 promotes exocytosis of damaged lysosomes through actin remodelling . The EMBO Journal.
Comprehensive Evaluation of Peripheral Immunodynamics in Advanced Non-Small Cell Lung Cancer Associated to Chemotherapy and Immunotherapy @article { author = {Rodrigues-Santos, Paulo and Sousa, Luana Madalena and Almeida, Jani Sofia and Barata, Fernando and Figueiredo, Ana}, title = {Comprehensive Evaluation of Peripheral Immunodynamics in Advanced Non-Small Cell Lung Cancer Associated to Chemotherapy and Immunotherapy}, journal = {Thorac Thoracic Cancer Journal}, volume = {1}, number = {2}, year = {2024}, url = {https://thoracjournal.com/index.php/tcj/article/view/53} } . Thorac Thoracic Cancer Journal.
Natural Killer T-like Cells: Immunobiology and Role in Disease @article { author = {Almeida, Jani-Sofia and Casanova, José Manuel and Santos-Rosa, Manuel and Tarazona, Raquel and Solana, Rafael and Rodrigues-Santos, Paulo}, title = {Natural Killer T-like Cells: Immunobiology and Role in Disease}, journal = {International Journal of Molecular Sciences}, volume = {24}, number = {3}, pages = {2743}, year = {2023}, month = {feb}, url = {http://dx.doi.org/10.3390/ijms24032743}, doi = {10.3390/ijms24032743} } . International Journal of Molecular Sciences.
Dynamics of Soluble Factors and Double-Negative T Cells Associated with Response to Renal Denervation in Resistant Hypertension Patients @article { author = {Joana Delgado-Silva and Paulo Rodrigues-Santos and Jani-Sofia Almeida and Manuel Santos-Rosa and Lino Gonçalves}, title = {Dynamics of Soluble Factors and Double-Negative T Cells Associated with Response to Renal Denervation in Resistant Hypertension Patients}, journal = {Journal of Personalized Medicine}, year = {2022}, month = {feb}, url = {https://doi.org/10.3390/jpm12030343}, doi = {10.3390/jpm12030343} } . Journal of Personalized Medicine.
Joana Delgado-Silva, Paulo Rodrigues-Santos, Jani-Sofia Almeida, Manuel Santos-Rosa, Lino Gonçalves (2022). Dynamics of Soluble Factors and Double-Negative T Cells Associated with Response to Renal Denervation in Resistant Hypertension Patients . Journal of Personalized Medicine.
Joana Delgado-Silva, Paulo Rodrigues-Santos, Jani-Sofia Almeida, Manuel Santos-Rosa, Lino Gonçalves (2022). Dynamics of Soluble Factors and Double-Negative T Cells Associated with Response to Renal Denervation in Resistant Hypertension Patients . Journal of Personalized Medicine.
Tumor-Infiltrating Lymphocytes and Cancer Markers in Osteosarcoma: Influence on Patient Survival @article { author = {Casanova, José Manuel and Almeida, Jani-Sofia and Reith, John David and Sousa, Luana Madalena and Fonseca, Ruben and Freitas-Tavares, Paulo and Santos-Rosa, Manuel and Rodrigues-Santos, Paulo}, title = {Tumor-Infiltrating Lymphocytes and Cancer Markers in Osteosarcoma: Influence on Patient Survival}, journal = {Cancers}, volume = {13}, number = {23}, pages = {6075}, year = {2021}, month = {dec}, url = {http://dx.doi.org/10.3390/cancers13236075}, doi = {10.3390/cancers13236075}, keywords = {Osteosarcoma; Tumor-infiltrating lymphocyte} } . Cancers.
Tumor and Peripheral Immune Status in Soft Tissue Sarcoma: Implications for Immunotherapy @article { author = {Sousa, Luana Madalena and Almeida, Jani Sofia and Fortes-Andrade, Tânia and Santos-Rosa, Manuel and Freitas-Tavares, Paulo and Casanova, José Manuel and Rodrigues-Santos, Paulo}, title = {Tumor and Peripheral Immune Status in Soft Tissue Sarcoma: Implications for Immunotherapy}, journal = {Cancers}, volume = {13}, number = {15}, pages = {3885}, year = {2021}, month = {aug}, url = {http://dx.doi.org/10.3390/cancers13153885}, doi = {10.3390/cancers13153885}, keywords = {Soft Tissue Sarcoma} } . Cancers.
The Role of Natural Killer Cells in Soft Tissue Sarcoma: Prospects for Immunotherapy @article { author = {Fortes-Andrade, Tânia and Almeida, Jani Sofia and Sousa, Luana Madalena and Santos-Rosa, Manuel and Freitas-Tavares, Paulo and Casanova, José Manuel and Rodrigues-Santos, Paulo}, title = {The Role of Natural Killer Cells in Soft Tissue Sarcoma: Prospects for Immunotherapy}, journal = {Cancers}, volume = {13}, number = {15}, pages = {3865}, year = {2021}, month = {jul}, url = {http://dx.doi.org/10.3390/cancers13153865}, doi = {10.3390/cancers13153865}, keywords = {Soft Tissue Sarcoma; Innate immunity} } . Cancers.
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth @article { author = {Mendes, João and Rodrigues-Santos, Paulo and Areia, Ana Luísa and Almeida, Jani-Sofia and Alves, Vera and Santos-Rosa, Manuel and Mota-Pinto, Anabela}, title = {Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth}, journal = {BMC Immunology}, volume = {22}, number = {1}, year = {2021}, month = {may}, url = {http://dx.doi.org/10.1186/s12865-021-00423-x}, doi = {10.1186/s12865-021-00423-x} } . BMC Immunology.
Immunotherapy and predictive immunologic profile: the tip of the iceberg @article { author = {Cunha Pereira, Tatiana and Rodrigues-Santos, Paulo and Almeida, Jani Sofia and Rêgo Salgueiro, Fábio and Monteiro, Ana Raquel and Macedo, Filipa and Soares, Rita Félix and et al.}, title = {Immunotherapy and predictive immunologic profile: the tip of the iceberg}, journal = {Medical Oncology}, volume = {38}, number = {5}, year = {2021}, month = {mar}, url = {http://dx.doi.org/10.1007/s12032-021-01497-8}, doi = {10.1007/s12032-021-01497-8} } . Medical Oncology.
Activated double-negative T cells (CD3+CD4-CD8-HLA-DR+) define response to renal denervation for resistant hypertension @article { author = {Delgado Silva, Joana and Almeida, Jani-Sofia and Rodrigues-Santos, Paulo and Santos Rosa, Manuel and Gonçalves, Lino}, title = {Activated double-negative T cells (CD3+CD4-CD8-HLA-DR+) define response to renal denervation for resistant hypertension}, journal = {Clinical Immunology}, volume = {218}, pages = {108521}, year = {2020}, month = {sep}, url = {http://dx.doi.org/10.1016/j.clim.2020.108521}, doi = {10.1016/j.clim.2020.108521} } . Clinical Immunology.
(2020). Activated double-negative T cells (CD3+CD4−CD8−HLA-DR+) define response to renal denervation for resistant hypertension . Clinical Immunology.
NKT-Like (CD3+CD56+) Cells in Chronic Myeloid Leukemia Patients Treated With Tyrosine Kinase Inhibitors @article { author = {Almeida, Jani-Sofia and Couceiro, Patrícia and López-Sejas, Nelson and Alves, Vera and Ružicková, Lenka and Tarazona, Raquel and Solana, Rafael and et al.}, title = {NKT-Like (CD3+CD56+) Cells in Chronic Myeloid Leukemia Patients Treated With Tyrosine Kinase Inhibitors}, journal = {Frontiers in Immunology}, volume = {10}, year = {2019}, month = {oct}, url = {http://dx.doi.org/10.3389/fimmu.2019.02493}, doi = {10.3389/fimmu.2019.02493}, keywords = {NKT-like; Chronic Myeloid Leukemia; Flow Cytometry} } . Frontiers in Immunology.
Effect of Age on NK Cell Compartment in Chronic Myeloid Leukemia Patients Treated With Tyrosine Kinase Inhibitors @article { author = {Rodrigues-Santos, Paulo and López-Sejas, Nelson and Almeida, Jani Sofia and Ruzicková, Lenka and Couceiro, Patricia and Alves, Vera and Campos, Carmen and et al.}, title = {Effect of Age on NK Cell Compartment in Chronic Myeloid Leukemia Patients Treated With Tyrosine Kinase Inhibitors}, journal = {Frontiers in Immunology}, volume = {9}, year = {2018}, month = {nov}, url = {http://dx.doi.org/10.3389/fimmu.2018.02587}, doi = {10.3389/fimmu.2018.02587}, keywords = {NK cells; Ageing; CMV; Leucemia Mieloide Crónica; TKI} } . Frontiers in Immunology.
CONFERENCE POSTER
Phenotypic Dynamics of Circulating gamma-delta T Cells During Pregnancy: The Impact of Maternal Age @misc { author = {Almeida, Jani and Alves, Vera and Vale-Pereira, Sofia and Mota-Pinto, A and Sá, Helena Oliveira and Areia, Ana Luísa}, title = {Phenotypic Dynamics of Circulating gamma-delta T Cells During Pregnancy: The Impact of Maternal Age}, year = {2025}, month = {apr}, keywords = {Gravidez/Pregnancy; Células T/T cells; Idade Materna/Maternal Age} }. SPI50 - L Annual Meeting - Sociedade Portuguesa de Imunologia "Breaking Boundaries in Immunology".
Perfis imunogenómicos periféricos associados a imunoterapia no CPNPC @misc { author = {Rodrigues-Santos, Paulo and Sousa, Luana Madalena and Almeida, Jani Sofia and Barata, Fernando and Figueiredo, Ana}, title = {Perfis imunogenómicos periféricos associados a imunoterapia no CPNPC}, year = {2024}, month = {oct} }. 2.º Congresso Português do Cancro do Pulmão I 11.º Congresso do GECP.
Neurovascular and immune alterations associated with psychostimulants use: a pre-clinical study @misc { author = {Fernandes, Ana Olívia and Ormonde, Amélia and Sanches, Eliane Swely and Valls, Rita and Silva, Ana Cristina and Almeida, Jani-Sofia and Alves, Vera and et al. Corresponding author: Ana Paula Silva.}, title = {Neurovascular and immune alterations associated with psychostimulants use: a pre-clinical study}, year = {2024}, month = {oct} }. XVIII Congresso Nacional de Psiquiatria.
CONFERENCE ABSTRACT
4P Immune exoproteome, soluble proteome and immune-related gene expression profiles of anti-PD-1 therapy in stage IIIB/IV non-small cell lung cancer: Relevance of immunosuppressive factors @inproceedings { author = {Santos, P.R. and Sousa, L.M. and Almeida, J.S. and Barata, F.J. and Figueiredo, A.M.}, title = {4P Immune exoproteome, soluble proteome and immune-related gene expression profiles of anti-PD-1 therapy in stage IIIB/IV non-small cell lung cancer: Relevance of immunosuppressive factors}, address = {Geneve}, volume = {20}, pages = {100475}, year = {2023}, month = {dec}, doi = {10.1016/j.iotech.2023.100475}, keywords = {Lung cancer} } . ESMO Immuno-Oncology Congress.
35P Peripheral immunotype classification for monitoring soft tissue sarcoma patients @inproceedings { author = {Almeida, J.S. and Sousa, L.M. and Rodrigues, J.M.C. and Fonseca, R. and Alves, V. and Santos-Rosa, M. and Tavares, P.F. and Casanova, J.M. and Santos, P.R.}, title = {35P Peripheral immunotype classification for monitoring soft tissue sarcoma patients}, address = {Geneve}, volume = {20}, pages = {100504}, year = {2023}, month = {dec}, doi = {10.1016/j.iotech.2023.100504}, keywords = {Immuno-oncology; Immune-monitoring; Soft Tissue Sarcoma} } . ESMO Immuno-Oncology Congress.
P054 Imunomodulação intestinal e benefícios metabólicos de uma fibra prebiótica proveniente de desperdícios agrícolas do mirtilo: ensaio em modelo animal de diabetes mellitus tipo 2. @inproceedings { author = {Pedro Vieira and Nunes, Sara and Preguiça, Inês and Ferreira, Carolina and Alves, André and Almeida, Jani Sofia and Helena Sá and et al.}, title = {P054 Imunomodulação intestinal e benefícios metabólicos de uma fibra prebiótica proveniente de desperdícios agrícolas do mirtilo: ensaio em modelo animal de diabetes mellitus tipo 2.}, year = {2023} }. 19º Congresso Português de Diabetes, 2023.
Cellular and plasmatic immune profile of anti-PD-1 therapy in phase IIIB/IV non-small cell lung cancer: relevance of immunosuppressive factors @inproceedings { author = {Rodrigues-Santos, P and Almeida, J S and Sousa, L M and Santos-Rosa, M and Barata, F and Figueiredo, A}, title = {Cellular and plasmatic immune profile of anti-PD-1 therapy in phase IIIB/IV non-small cell lung cancer: relevance of immunosuppressive factors}, address = {Barcelona}, year = {2022}, doi = {10.1183/13993003.congress-2022.987}, keywords = {Lung cancer} } . European Respiratory Society Congress.
MONITORING IMMUNODYNAMICS IN IMMUNE CHECKPOINT INHIBITOR THERAPY FOR STAGE IIIB/IV NON-SMALL CELL LUNG CANCER @inproceedings { author = {Bento, Daniela and Almeida, Jani Sofia and Couceiro, Patrícia and Alves, Vera and Santos-Rosa, Manuel and Barata, Fernando José and Rodrigues-Santos, Paulo and Ferreira Rodrigues Figueiredo, Ana Maria}, title = {MONITORING IMMUNODYNAMICS IN IMMUNE CHECKPOINT INHIBITOR THERAPY FOR STAGE IIIB/IV NON-SMALL CELL LUNG CANCER}, address = {Paris}, year = {2018}, month = {sep}, doi = {10.1183/13993003.congress-2018.pa2226} } . European Respiratory Society Congress.