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USD 40 /hr

★★★★★

☆☆☆☆☆

Hire Dr. Jacob W.

United States

USD 40 /hr

Helping scientists design better experiments, analyze data rigorously, and publish clearer figures.

Profile Summary

Peptide Chemist | Expert in SPPS • Recently graduated PhD chemist at the University of Pittsburgh in the lab of Seth Horne for 6 years. • Specialized in long and difficult peptide sequences. • Synthesized 15-mer protease substrates, 30 to 45-mer miniproteins, and 62-mer natural enzyme. • Experience with both manual and automated synthesis tools: Sonication Bath, CEM Microwave Synthesizer, Biotage Initiator+ Alstra, and Gyros PepPure Chorus. Structural Biologist | Expert in Biophysical Characterization • Recently Graduated PhD chemist at the University of Pittsburgh in the Lab of Seth Horne for 6 years and at Franklin & Marshall College in the lab of Gabriel Brandt. • Protein and peptide production, purification, and identity confirmation via E. coli and SPPS. • Experience with the following purification techniques: SEC/GPC, Ion Exchange Chromatography, Analytical and Preparatory HPLC, SDS PAGE Gels, Nickel Affinity Chromatography, Dialysis and Filtration. • Experience with the following purity/identity confirmation techniques: Analytical HPLC, Analytical SEC, ESI Mass Spectrometry, MALDI Mass Spectrometry. • Experience with the following biophysical characterization/design techniques: Binding Assays, Circular Dichroism, Thermal Shift Assay and Differential Scanning Fluorimetry, SAXS, 1H NMR, UV-Vis Spectroscopy, Fluorescence Spectroscopy (TECAN M1000 Automation), MD Simulation, AutoDockVina, AlphaFold 3, RF Diffusion (De Novo Design via Protein Generator), Enzyme Kinetic Assays, Enzyme Inhibition Assays and Plate-Based Screening. LinkedIn Consultant | Expert in Science Communication and Academic Social Media Presence • Founder of Peptide Chemistry Journal Club LinkedIn Group (~2100 Members) • Last Week In Peptide Chemistry Newsletter (~1400 Subscribers) • How To Get More Out of Networking and Posting Experimental Design and Data Analysis Consultant | Expert in R, Python, Excel, and GraphPad Prism • Patient and supportive mentor with 10 years of experience working with and training Undergraduate scientists. • Excellent UX-Oriented workflow development.

Subject Matter Expertise

• ★ Separation & Purification
• ★ Peptide Synthesis
• ★ Biophysics
• ☆ Inorganic Chemistry
• ☆ Analytical Chemistry
• ☆ Crystallography
• ☆ X-Ray Crystallography
• ☆ Spectroscopy
• ☆ UV Spectroscopy
• ☆ Fluorescence Spectroscopy
• ☆ NMR Spectroscopy
• ☆ Circular Dichroism Spectroscopy
• ☆ Mass Spectrometry
• ☆ Liquid Chromatography/HPLC
• ☆ Computational Chemistry
• ☆ Computational Biology
• ☆ Biochemistry
• ☆ Biochemical Assays
• ☆ Structural Biology
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Information Technology
• ☆ Database Management
• ☆ Automation
• ☆ Data Analysis
• ☆ Image Processing
• ☆ Computer Simulation/Computational Modeling
• ☆ Data Processing
• ☆ Data Exploration
• ☆ Data Visualization
• ☆ Data Management
• ☆ Data Manipulation
• ☆ Text Mining & Analytics
• ☆ Data Cleaning
• ☆ Bioinformatics
• ☆ Gene & Protein Expression Analysis
• ☆ Biomolecular Engineering
• ☆ Protein/Enzyme Engineering
• ☆ Publishing
• ☆ Python
• ☆ Excel
• ☆ R
• ☆ Drug Discovery
• ☆ High Throughput Screening (HTS)
• ☆ UX Design
• ☆ Technical & Scientific Writing
• ☆ Science Communication
• ☆ Literature Review
• ☆ Systematic Literature Review
• ☆ Grant Writing

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Medical Writing, Technical Writing, Copywriting, Creative Writing, Newswriting, General Proofreading & Editing

Research User Research, Meta-Research, Technology Scouting, Fact Checking, Gap Analysis, Scientific and Technical Research, Systematic Literature Review, Secondary Data Collection

Consulting Business Strategy Consulting, Digital Strategy Consulting, Operations Consulting, Scientific and Technical Consulting

Data & AI Statistical Analysis, Image Processing, Data Visualization, Text Mining & Analytics, Data Processing, Data Insights

Product Development Formulation, Recipe Development, Concept Development, Prototyping

Work Experience

Graduate Student

University of Pittsburgh

August 2019 - Present

Education

Doctorate

University of Pittsburgh

August 2019 - December 2025

Certifications

• Doctorate

  University of Pittsburgh

  August 2019 - December 2025

Publications

JOURNAL ARTICLE

Mariah C. Meehan, Grace E. Scofield, Corrinne E. Stahl, Jacob A. Wolfe, W. Seth Horne, Peng Liu, Kay M. Brummond (2025). A Predictive Model for Thiol Reactivity of N-Heteroaryl α-Methylene−γ-Lactams─A Medicinally Relevant Covalent Reactive Group . Journal of Medicinal Chemistry.

Jacob A. Wolfe, W. Seth Horne (2024). Application of artificial backbone connectivity in the development of metalloenzyme mimics . Current Opinion in Chemical Biology.

Klootwyk, Brittany M., et al. "Peroxide-Mediated Release of Organophosphates from Boron-Containing Phosphotriesters: A New Class of Organophosphate Prodrugs." Organic Letters 25.29 (2023): 5530-5535 .

Jacob Wolfe, Shilpa R. Rao, Thomas W. Harmon, Shelby L. Heath, Jacob A. Wolfe, Jacqueline R. Santhouse, Gregory L. O'Brien, Alexis N. Distefano, Zachary E. Reinert, W. Seth Horne(2022). Chemical shifts of artificial monomers used to construct heterogeneous‐backbone protein mimetics in random coil and folded states . Peptide Science. Wiley

Astrid M. Perez, Jacob A. Wolfe, Janse T. Schermerhorn, Yiwen Qian, Bekim A. Cela, Cody R. Kalinowski, Garrett E. Largoza, Peter A. Fields, Gabriel S. Brandt (2021). Thermal stability and structure of glyceraldehyde-3-phosphate dehydrogenase from the coral Acropora millepora . RSC Advances.

PREPRINT

Mariah Meehan, Grace Scofield, Corrinne Stahl, Jacob Wolfe, W. Seth Horne, Peng Liu, Kay Brummond (2025). A predictive model for thiol reactivity of N-heteroaryl α-methylene–γ-lactams—a medicinally relevant covalent reac-tive group .

Jacob Wolfe, Mikaela Luksic, Rebecca Gonda (2023). Making Sunscreen with Household Chemicals - A Frugal Demonstration of the Aldol Clock Reaction .