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Medical Writer | Regulatory Documents (IBs, CSRs) | Digital Health & AI-Driven Diagnostics | Fast, Publication-Ready
Profile Summary
Subject Matter Expertise
Services
Work Experience
Founder/CSO
CleverLab
January 2025 - Present
Academic writer
Upwork
November 2021 - December 2024
Post-Doc
Universidade do Porto Faculdade de Engenharia
February 2014 - November 2021 
Education
PhD in Chemical and Biological Engineering
Universidade do Minho
2007 - 2011
Master Degree in Environmental Management
Universidade do Minho
2004 - 2006
Degree in Biological Engineering
Universidade do Minho
1997 - 2003
Certifications
Publications
JOURNAL ARTICLE
Idalina Machado, Mariana Sousa, Lúcia C. Simões, Manuel Simões (2025). Biocides as drivers of antibiotic resistance: A critical review of environmental implications and public health risks . Environmental Science and Ecotechnology.
Idalina Machado, Maria Sousa-Silva, Manuel Simões, Luís Melo(2018). Pseudomonas fluorescens tolerance to benzyldimethyldodecyl ammonium chloride: Altered phenotype and cross-resistance . Journal of Global Antimicrobial Resistance. 15. p. 188--195. Elsevier {BV}
(2018). Pseudomonas fluorescens tolerance to benzyldimethyldodecyl ammonium chloride: altered phenotype and cross-resistance . Journal of global antimicrobial resistance.
Idalina Machado, Paula A. Araújo, Ana Meireles, TorOve Leiknes, Filipe Mergulhão, Luís F. Melo, Manuel Simões(2017). Combination of selected enzymes with cetyltrimethylammonium bromide in biofilm inactivation, removal and regrowth . Food Research International. 95. p. 101--107. Elsevier {BV}
Idalina Machado, Ana Meireles, Rita Fulgêncio, Filipe Mergulhão, Luís Melo, Manuel Simões(2017). Characterization of the heterotrophic bacteria from a minimally processed vegetables plant . LWT - Food Science and Technology. Elsevier {BV}
Idalina Machado, Paula A. Araújo, Joana Malheiro, Filipe Mergulhão, Luís Melo, Manuel Simões(2016). Influence of Flow Velocity on the Characteristics ofPseudomonas fluorescensBiofilms. J. Environ. Eng. 142. (7). p. 04016031. American Society of Civil Engineers ({ASCE})
Influence of Flow Velocity on the Characteristics ofPseudomonas fluorescensBiofilms @article {
author = {Paula A. Araújo and Joana Malheiro and Idalina Machado and Filipe Mergulhão and Luís Melo and Manuel Simões},
title = {Influence of Flow Velocity on the Characteristics ofPseudomonas fluorescensBiofilms},
journal = {J. Environ. Eng.},
volume = {142},
number = {7},
pages = {04016031--04016031},
year = {2016},
month = {jul},
url = {http://dx.doi.org/10.1061/(asce)ee.1943-7870.0001068},
doi = {10.1061/(asce)ee.1943-7870.0001068}
}. J. Environ. Eng.
Idalina Machado, Joana Sampaio, Diana Machado, Ana Marta Gomes, Cledir Santos, Nelson Lima, Maria João Carvalho, António Cabrita, Anabela Rodrigues, Margarida Martins, et al.(2016). Deciphering the Contribution of Biofilm to the Pathogenesis of Peritoneal Dialysis Infections: Characterization and Microbial Behaviour on Dialysis Fluids . PLoS ONE. 11. (6). p. e0157870. Public Library of Science ({PLoS})
J.M.R. Moreira, R. Fulgêncio, P. Alves, I. Machado, I. Bialuch, L.F. Melo, M. Simões, F.J. Mergulhão(2016). Evaluation of SICAN performance for biofouling mitigation in the food industry . Food Control. 62. p. 201--207. Elsevier {BV}
I. Machado, A. Meireles, R. Fulgêncio, F. Mergulhão, M. Simões, L.F. Melo(2016). Disinfection with neutral electrolyzed oxidizing water to reduce microbial load and to prevent biofilm regrowth in the processing of fresh-cut vegetables . Food and Bioproducts Processing. 98. p. 333--340. Elsevier {BV}
J.M.R. Moreira, R. Fulgêncio, F. Oliveira, I. Machado, I. Bialuch, L.F. Melo, M. Simões, F.J. Mergulhão(2016). Evaluation of SICON® surfaces for biofouling mitigation in critical process areas . Food and Bioproducts Processing. 98. p. 173--180. Elsevier {BV}
Idalina Machado, Laurent Coquet(2016). Proteomic Changes in Pseudomonas aeruginosa Biofilm Cells after Adaptive Resistance Development . J Proteomics Bioinform. 09. (03). {OMICS} Publishing Group
J. Malheiro, P. Araújo, I. Machado, M. Lemos, F. Mergulhão, L. Melo, M. Simões(2015). The Effects of Selected Brominated and Chlorinated Chemicals onPseudomonas fluorescensPlanktonic Cells and Flow-Generated Biofilms . Journal of Food Processing and Preservation. 40. (2). p. 316--328. Wiley-Blackwell
A.L. Amaro, I. Machado, M.J. Pereira, S. Carvalho, M.F. Poças, M. Vasconcelos, M. Pintado(2015). RESPIRATION RATE AND FUNCTIONAL QUALITY OF ROCKET LEAVES AS AFFECTED BY PASSIVE MODIFIED ATMOSPHERE PACKAGING . Acta Hortic. (1071). p. 717--724. International Society for Horticultural Science ({ISHS})
Idalina Machado, Ana Meireles, Rita Fulgêncio, Filipe Mergulhão, Luís Melo, Manuel Simões(2015). Efficacy of antimicrobial combinations to reduce the use of sodium hypochlorite in the control of planktonic and sessile Escherichia coli . Biochemical Engineering Journal. Elsevier {BV}
N.D. Spadafora, I. Machado, C.T. Müller, M. Pintado, M. Bates, H.J. Rogers(2015). PHYSIOLOGICAL, METABOLITE AND VOLATILE ANALYSIS OF CUT SIZE IN MELON DURING POSTHARVEST STORAGE . Acta Hortic. (1071). p. 787--793. International Society for Horticultural Science ({ISHS})
Involvement of bolA and rpoS genes in oxygen uptake (respirometry) by E. coli K-12 MG1655 under various stress conditions @article {
author = {Adnan, M. and Machado, I. and Sousa, A.M. and Pereira, M.O. and Morton, G. and Hadi, S.},
title = {Involvement of bolA and rpoS genes in oxygen uptake (respirometry) by E. coli K-12 MG1655 under various stress conditions},
journal = {Asian Journal of Microbiology, Biotechnology and Environmental Sciences},
volume = {15},
number = {1},
pages = {19--24},
year = {2013},
url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84878037691&partnerID=MN8TOARS}
} . Asian Journal of Microbiology, Biotechnology and Environmental Sciences.
Machado, I., Coquet, L., Jouenne, T., Pereira, M.O.(2013). Proteomic approach to pseudomonas aeruginosa adaptive resistance to benzalkonium chloride . Journal of Proteomics. 89. p. 273-279.
Sousa, A.M., Machado, I., Nicolau, A., Pereira, M.O.(2013). Improvements on colony morphology identification towards bacterial profiling . Journal of Microbiological Methods. 95. (3). p. 327-335.
Adnan, M., Machado, I., Sousa, A.M., Pereira, M.O., Morton, G., Hadi, S.(2013). Involvement of bolA and rpoS genes in oxygen uptake (respirometry) by E. coli K-12 MG1655 under various stress conditions . Asian Journal of Microbiology, Biotechnology and Environmental Sciences. 15. (1). p. 19-24.
Lourenço, A., Ferreira, A., Veiga, N., Machado, I., Pereira, M.O., Azevedo, N.F.(2012). Biofomics: A web platform for the systematic and standardized collection of high-throughput biofilm data . PLoS ONE. 7. (6).
Machado, I., Lopes, S.P., Sousa, A.M., Pereira, M.O.(2012). Adaptive response of single and binary Pseudomonas aeruginosa and Escherichia coli biofilms to benzalkonium chloride . Journal of Basic Microbiology. 52. (1). p. 43-52.
Machado, I., Graça, J., Sousa, A.M., Lopes, S.P., Pereira, M.O.(2011). Effect of antimicrobial residues on early adhesion and biofilm formation by wild-type and benzalkonium chloride-adapted Pseudomonas aeruginosa . Biofouling. 27. (10). p. 1151-1159.
Lopes, S.P., MacHado, I., Pereira, M.O.(2011). Role of planktonic and sessile extracellular metabolic byproducts on Pseudomonas aeruginosa and Escherichia coli intra and interspecies relationships . Journal of Industrial Microbiology and Biotechnology. 38. (1). p. 133-140.
Simões, M., Simões, L.C., Cleto, S., Machado, I., Pereira, M.O., Vieira, M.J.(2007). Antimicrobial mechanisms of ortho-phthalaldehyde action . Journal of Basic Microbiology. 47. (3). p. 230-242.
Simões, M., Simões, L.C., Machado, I., Pereira, M.O., Vieira, M.J.(2006). Control of flow-generated biofilms with surfactants: Evidence of resistance and recovery . Food and Bioproducts Processing. 84. (4 C). p. 338-345.
Simões, M., Pereira, M.O., Machado, I., Simões, L.C., Vieira, M.J.(2006). Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens . Journal of Industrial Microbiology and Biotechnology. 33. (9). p. 741-749.
CONFERENCE POSTER
Biocide coated microparticles as anti-biofilm agents @misc {
author = {Machado, I.},
title = {Biocide coated microparticles as anti-biofilm agents},
year = {2016},
month = {jun}
}. Biofilms 7.
Evaluation of SICAN and SICON® surfaces for biofouling mitigation in the food industry @misc {
author = {Machado, I.},
title = {Evaluation of SICAN and SICON® surfaces for biofouling mitigation in the food industry},
year = {2016},
month = {jun}
}. Biofilms 7.
Electrochlorinated water as surface cleaning agent: in vitro approach @misc {
author = {Machado, I.},
title = {Electrochlorinated water as surface cleaning agent: in vitro approach},
year = {2014},
month = {dec}
}. Susclean Final Meeting.
Microbial population dynamics after sanitation with neutral electrolysed oxidizing water @misc {
author = {Machado, I.},
title = {Microbial population dynamics after sanitation with neutral electrolysed oxidizing water},
year = {2014},
month = {dec}
}. Susclean Final Meeting.
Characterisation of a bacterium isolated from a minimally processed vegetables plant including its antimicrobial resistanc @misc {
author = {Machado, I.},
title = {Characterisation of a bacterium isolated from a minimally processed vegetables plant including its antimicrobial resistanc},
year = {2014},
month = {sep}
}. 12th International Chemical and Biological Engineering Conference (Chempor 2014).
Blocking of Pseudomonas aeruginosa biofilm formation by a colistin coating @misc {
author = {Machado, I.},
title = {Blocking of Pseudomonas aeruginosa biofilm formation by a colistin coating},
year = {2012},
month = {nov}
}. II International Conference on Antimicrobial Research -ICAR2012.
Exploring different preventive strategies to control Pseudomonas aeruginosa biofilms using Colistin @misc {
author = {Machado, I.},
title = {Exploring different preventive strategies to control Pseudomonas aeruginosa biofilms using Colistin},
year = {2012},
month = {jun}
}. Third International Symposium on Antimicrobial Peptides.
OMP proteomic analysis of benzalkonium chloride and ciprofloxacin adapted biofilm cells @misc {
author = {Machado, I.},
title = {OMP proteomic analysis of benzalkonium chloride and ciprofloxacin adapted biofilm cells},
year = {2010},
month = {sep}
}. Conference Handbook of the 4th International Conference Biofilms IV.
Susceptibility patterns and cross-resistance evaluation of several biofilm-producing P. aeruginosa challenged by antibiotics @misc {
author = {Machado, I.},
title = {Susceptibility patterns and cross-resistance evaluation of several biofilm-producing P. aeruginosa challenged by antibiotics},
year = {2010},
month = {sep}
}. 4th International Conference Biofilms IV: Communities Bridging Disciplines.
Development of a web-based platform for the systematic and large-scale study of microbial adhesion and biofilms. @misc {
author = {Machado, I.},
title = {Development of a web-based platform for the systematic and large-scale study of microbial adhesion and biofilms.},
year = {2010},
month = {sep}
}. Conference Handbook of the 4th International Conference Biofilms IV.
Biological cost of antibiotic pressure in Pseudomonas aeruginosa biofilm phenotype @misc {
author = {Machado, I.},
title = {Biological cost of antibiotic pressure in Pseudomonas aeruginosa biofilm phenotype},
year = {2010},
month = {apr}
}. 20th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID).
Binary biofilm behaviour: sum of the synergistic and antagonistic interactions. @misc {
author = {Machado, I.},
title = {Binary biofilm behaviour: sum of the synergistic and antagonistic interactions.},
year = {2009},
month = {nov}
}. Microbiotec09.
Adaptive response of single and binary biofilms formed in the presence of benzalkonium chloride @misc {
author = {Machado, I.},
title = {Adaptive response of single and binary biofilms formed in the presence of benzalkonium chloride},
year = {2009},
month = {nov}
}. 5th ASM Conference on Biofilms.
Influence of ionic strength in P. aeruginosa early-biofilm development @misc {
author = {Machado, Idalina and Cunha, Rita and Cerqueira, L. and Lopes, Susana Patrícia and Azevedo, N. F. and Vieira, M. J. and Pereira, Maria Olívia},
title = {Influence of ionic strength in P. aeruginosa early-biofilm development},
year = {2009},
month = {sep}
}. Eurobiofilms: First European Congress on Microbial Biofilms.
Pseudomonas aeruginosa antimicrobial resistance and cross resistance: the role of sessile growth and induced tolerance. @misc {
author = {Machado, I.},
title = {Pseudomonas aeruginosa antimicrobial resistance and cross resistance: the role of sessile growth and induced tolerance.},
year = {2009},
month = {sep}
}. Eurobiofilms: First European Congress on Microbial Biofilms.
Effect of benzalkonium chloride residues on the initial adhesion of adapted Pseudomonas aeruginosa @misc {
author = {Machado, I.},
title = {Effect of benzalkonium chloride residues on the initial adhesion of adapted Pseudomonas aeruginosa},
year = {2008},
month = {oct}
}. BIOFILMS III, 3 rd International Conference.
Comparison of the antimicrobial efficacy of commercial surfactants with the respective pure components. @misc {
author = {Machado, I. and Machado, Idalina and Ramos, Carla and Simões, M. and Vieira, M. J. and Pereira, Maria Olívia},
title = {Comparison of the antimicrobial efficacy of commercial surfactants with the respective pure components.},
year = {2008},
month = {sep},
keywords = {Surfactants; Antimicrobial action; Sanitation; Environmental impact; Mixed bacterial growth; Bacterial resistance}
}. Chempor- 10 th International Chemical and Biological Engineering Conference.
Dispersion of biofilm mass and activity values : sign of hypermutation in P. aeruginosa biofilms? @misc {
author = {Machado, Idalina and Simões, M. and Vieira, M. J. and Pereira, Maria Olívia},
title = {Dispersion of biofilm mass and activity values : sign of hypermutation in P. aeruginosa biofilms?},
year = {2008},
month = {aug}
}. 12nd International Symposium on Microbial Ecology.
Control of mixed biofilms with benzalkonium chloride : effect of different application strategies @misc {
author = {Machado, Idalina and Rodrigues, A. C. and Pereira, Maria Olívia},
title = {Control of mixed biofilms with benzalkonium chloride : effect of different application strategies},
year = {2008}
}. International Biofilm conference III.
Effect of benzalkonium chloride residues on the initial adhesion of adapted P. aeruginosa @misc {
author = {Machado, Idalina and Vieira, M. J. and Pereira, Maria Olívia},
title = {Effect of benzalkonium chloride residues on the initial adhesion of adapted P. aeruginosa},
year = {2008}
}. Biofilms III.
Persisters, in the news yesterday, on our labs today: the world together for a safer tomorrow @misc {
author = {Ana Meireles and Rita Fulgêncio and Idalina Machado and Filipe Mergulhão and Luís Melo and Manuel Simões},
title = {Persisters, in the news yesterday, on our labs today: the world together for a safer tomorrow},
year = {2007},
month = {nov},
doi = {10.1016/j.lwt.2017.01.038}
} . Biofilms, Hypermutability and Antibiotic Resistance.
Use of CV and XTT to screen biofilm susceptibility to new QAC's compounds @misc {
author = {Machado, Idalina and Simões, M. and Pereira, Maria Olívia and Guittard, Frederic and Guittard, Elisabeth and Vieira, M. J. and Machado, I.},
title = {Use of CV and XTT to screen biofilm susceptibility to new QAC's compounds},
year = {2006},
month = {mar}
}. International conference biofilms ii : attachment and detachment in pure and mixed cultures.
BOOK
Book of Abstracts of Biofilms 7 Microbial works of art @book {
author = {Machado, I.},
title = {Book of Abstracts of Biofilms 7 Microbial works of art},
publisher = {Melo L, Azeredo J, Pereira MO, Mergulhão F, Henriques M, Simões M, Cerca N, Azevedo N, Machado I},
year = {2016}
}. Melo L, Azeredo J, Pereira MO, Mergulhão F, Henriques M, Simões M, Cerca N, Azevedo N, Machado I.
CONFERENCE PAPER
(2015). Neutral Electrolysed Oxidizing Water as Anti-Biofouling Alternative to Traditional Chlorine. Proceedings - Heat Exchanger Fouling and Cleaning XI - 2015.
Neutral electrolysed oxidizing water as anti-biofouling alternative to traditional chlorine @inproceedings {
author = {Machado, I.},
title = {Neutral electrolysed oxidizing water as anti-biofouling alternative to traditional chlorine},
year = {2015},
month = {jun},
booktitle = {Proceedings of International Conference on Heat Exchanger Fouling and Cleaning - 2015 }
}. Heat Exchanger Fouling and Cleaning XI.
N.D. Spadafora and I. Machado and C.T. Müller and M. Pintado and M. Bates and H.J. Rogers(2015). Physiological, Metabolite and Volatile Analysis of Cut Size in Melon during Postharvest Storage . Acta Hortic. (1071). p. 787--793. International Society for Horticultural Science ({ISHS})
A.L. Amaro and I. Machado and M.J. Pereira and S. Carvalho and M.F. Po{\c{c}}as and M. Vasconcelos and M. Pintado(2015). Respiration rate and functional quality of rocket leaves as affected by passive modified atmosphere packaging . Acta Hortic. (1071). p. 717--724. International Society for Horticultural Science ({ISHS})
Respiration rate and functional quality of rocket leaves as affected by passive modified atmosphere packaging @inproceedings {
author = {Machado, Idalina},
title = {Respiration rate and functional quality of rocket leaves as affected by passive modified atmosphere packaging},
year = {2013},
month = {jun},
booktitle = {Acta Horticulturae 1071},
pages = {717--724},
publisher = {International Society for Horticultural Science },
doi = {10.17660/actahortic.2015.1071.95}
} . XIth Int. Controlled and Modified Atmosphere Research Conference.
Control of flow generated biofilms with surfactants - evidence of resistance and recovery @inproceedings {
author = {Machado, Idalina and Simões, M. and Simões, Lúcia C. and Pereira, Maria Olívia and Vieira, Maria João},
title = {Control of flow generated biofilms with surfactants - evidence of resistance and recovery},
year = {2006},
booktitle = {Proceedings of the International Conference Fouling, Cleaning and Disinfection in Food Processing},
doi = {10.1205/fbp06022},
keywords = {Biofilm control; Recovery; Resistance; Hydrodynamic conditions; Surfactant; Science & Technology}
} . International Conference Fouling, Cleaning and Disinfection in Food Processing.
(2006). Control of flow generated biofilms with surfactants - evidence of resistance and recovery. Proceedings of the International Conference Fouling, Cleaning and Disinfection in Food Processing.
(2006). Influence of material type and surface benzalkonium chloride preconditioning on biofilm formation and activity. Proceedings of the International Conference Fouling, Cleaning and Disinfection in Food Processing.
(2006). Control of flow generated biofilms with surfactants - evidence of resistance and recovery. Proceedings of the International Conference Fouling, Cleaning and Disinfection in Food Processing.
BOOK CHAPTER
Idalina Machado and Joana Gra{\c{c}}a and H{\'{e}}lder Lopes and Susana Lopes and Maria Pereira(2014). Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa . Biofilm Control and Antimicrobial Agents. p. 169--190. Informa {UK} Limited
Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Bio¿lm Development by an Endoscope-Isolated Pseudomonas aeruginosa @inbook {
author = {Machado, I.},
title = {Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Bio¿lm Development by an Endoscope-Isolated Pseudomonas aeruginosa},
year = {2014}
}. Biofilm control and antimicrobial agents.
Aquisição e Análise de Dados de Biofilme em Larga Escala @inbook {
author = {Machado, Idalina},
title = {Aquisição e Análise de Dados de Biofilme em Larga Escala},
year = {2012}
}.
(2012). Aquisição e Análise de Dados de Biofilme em Larga Escala. Biofilmes – Na Saúde, No Ambiente, Na Indústria.
(2011). Phenotypic switching: an opportunity to bacteria thrive. Science against microbial pathogens: communicating current research and technological advances.
Phenotypic switching: an opportunity to bacteria thrive @inbook {
author = {Machado, Idalina},
title = {Phenotypic switching: an opportunity to bacteria thrive },
publisher = {Formatex Research Center},
year = {2011}
}. Science against microbial pathogens: communicating current research and technological advances.
Preventing Biofilm Formation Using Surfactants @inbook {
author = {Machado, Idalina},
title = {Preventing Biofilm Formation Using Surfactants},
year = {2007}
}.
(2007). Preventing Biofilm Formation Using Surfactants. Bolfilms: coming of age.
OTHER
Idalina Machado, Joana Graça, Hélder Lopes, Susana Lopes, Maria Pereira(2014). Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa . Biofilm Control and Antimicrobial Agents. p. 169--190. Informa {UK} Limited
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