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Hire Dr. Heramb K.

India

Profile Summary

 Biological mass spectrometry (MALDI-TOF/TOF AB4800, Thermo LTQ XL, Thermo Orbitrap velos, ESI-triple quadruple-TOF AB5600) high throughput proteomics (qualitative and quantitative) including wet lab and dry lab (Series 4000, Proteome Discoverer, Protein Pilot, Peaks, Thermo Excalibur, AB Sciex Analyst, BiopharmaView and many related platforms) Identification of post-translational modifications of proteins/mABs, Manipulations of spectra by chemical modifications of proteins/peptides, mass spectrometric identification of lipids, GC-MS FAMEs analysis, lipidomics.  Spectroscopic methods, viz. UV-visible spectrophotometry, fluorescence spectrometry, Dynamic light scattering for particle size and zeta potential measurements.  Protein/mABs Purification by HPLC, UHPLC, affinity, ion-exchange, size exclusion, dialysis  SDS-PAGE, Isoelectric focusing, 2D-gel electrophoresis, Western Blotting, antibody based pull-down assays, identification of interacting partners of proteins, finding components of complexes, ELISA based assays.  Aseptic handling, Animal cell culture techniques, Formation of hybridoma cells from mouse spleen cells, Selection of clones, Cell based assays, Immunostaining.  Microbiological techniques, handling of pathogens at Bio-safety III level, maintenance and storage of bacterial cultures, Plate count and growth curve based assays, Staining methods, Antibiotic sensitivities, Characterization of microbial strains by classic and 16s-rRNA methods.  Ultracentrifugation techniques (preparative and analytical), Density gradient ultracentrifugation, Purification of virus particles and other tiny (nano-scale) particles.  Light microscopy, fluorescence microscopy, Scanning electron microscopy, Transmission electron microscopy, Cryo-Electron Microscopy.

Subject Matter Expertise

• ☆ Separation & Purification
• ☆ Mass Spectrometry
• ☆ Liquid Chromatography/HPLC
• ☆ Peptide Synthesis
• ☆ Biology/Life Sciences
• ☆ Microbiology
• ☆ Biochemical Assays
• ☆ Biotechnology & Bioengineering
• ☆ Microscopy
• ☆ Protein/Enzyme Engineering
• ☆ Antibody Engineering
• ☆ Blotting
• ☆ Antibiotic Development

Services

• Writing
• Consulting
• Product Development

Work Experience

Research Scientist

Accutest Research Laboratories India Private Limited

October 2015 - Present

Research Associate (Postdoctoral)

Centre for Cellular and Molecular Biology CSIR

August 2014 - September 2015

Research Scholar (Ph.D. Student)

CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY, HYDERABAD

August 2008 - July 2014

Education

Ph.D.

Centre for Cellular and Molecular Biology CSIR

August 2008 - July 2014

Master of Science (Biotechnology)

Shivaji University

June 2006 - April 2008

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2014). Biogenesis and multifaceted roles of outer membrane vesicles from Gram-negative bacteria . Microbiology.

Kulkarni, Heramb M., et al. "Acetylating Tryptic peptides enhances b Ion Intensity in MALDI TOF/TOF: Implications in peptide sequencing and Identification of Proteins in an Antarctic Bacterium Pseudomonas Syringae." Proteomics Insights 3 (2010): PRI-S3676.

Fulsundar, Shweta, et al. "Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis." Microbial pathogenesis 83 (2015): 12-22.

Kulkarni, Heramb M., R. Nagaraj, and Medicharla V. Jagannadham. "Protective role of E. coli outer membrane vesicles against antibiotics." Microbiological research 181 (2015): 1-7.

Kulkarni, Heramb M., Ch VB Swamy, and Medicharla V. Jagannadham. "Molecular characterization and functional analysis of outer membrane vesicles from the antarctic bacterium Pseudomonas syringae suggest a possible response to environmental conditions." Journal of proteome research 13.3 (2014): 1345-1358.

Jagannadham, Medicharla V., Ehab F. Abou-Eladab, and Heramb M. Kulkarni. "Identification of outer membrane proteins from an Antarctic bacterium Pseudomonas syringae Lz4W." Molecular & Cellular Proteomics 10.6 (2011): M110-004549.