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Hire Dr. Gaurav S.

France

USD 50 /hr

My core expertise lies in plant cell biology with advanced skills in live imaging, microfluidic

Profile Summary

I am Gaurav Singh, holding a PhD in Biotechnology, with a robust academic and research background spanning over a decade. My educational journey began with a B.Sc. in Biology from Dr. R.M.L. Avadh University, India (2005-2008), followed by an M.Sc. in Biotechnology from Punjab Technical University, India (2008-2010). I earned my PhD from Banaras Hindu University, India, in collaboration with CSIR-NBRI, Lucknow, completing my thesis in 2017 on the “Functional analysis of sterol glycosyltransferase gene (SGTs) family members of Withania somnifera using artificial miRNA technology.” Research Experience My research career is marked by diverse and impactful projects, primarily focused on plant biology, mechanotransduction, and molecular genetics: • Postdoctoral Fellow, BIAM-CEA, France (06/2022–12/2024): Currently, I am engaged in two significant projects. The first explores the ion flux network in Arabidopsis guard cells to regulate gas exchange, while the second investigates the STOP1 transcription factor’s regulation by cytosolic acidification in Arabidopsis. These projects leverage advanced imaging and molecular techniques to unravel plant physiological responses. • Postdoctoral Fellow, IBMP, University of Strasbourg, France ): Here, I worked on integrating mechanotransduction in plant development, specifically how cell shape influences chromatin remodeling. This interdisciplinary project combined biological and biomechanical approaches. • Visiting Academic, SLS, University of Warwick, United Kingdom ): I studied the structure and mechanism of action of PIN proteins, key auxin efflux transporters in plants, contributing to understanding auxin-mediated growth regulation. Skills I possess a wide array of technical skills critical to my research: • Imaging and Microscopy: Proficient in live imaging, confocal microscopy (LSM700, LSM780, LSM 980 Airy scan), and spinning disk microscopy. • Molecular Biology: Experienced in Gateway cloning, Virus-Induced Gene Silencing, CRISPR-Cas9 gene editing, PCR, real-time PCR, immunolabeling, protein purification, and Western blotting. • Plant Biotechnology: Skilled in plant tissue culture, transgenic preparation, and scintillation counting. • Computational Tools: Competent in ImageJ, SnapGene, and GraphPad Prism for data analysis. • Cell Culture: Handled insect cell cultures (Sf9 and High Five). • Languages: Fluent in English and Hindi, with ongoing learning of French. Additionally, I have editorial experience as an associate editor for Frontiers in Agronomy and serve as a reviewer for prestigious journals like Plant Physiology and Biochemistry and Frontiers in Plant Sciences. I have collaborated on HFSP and ANR-funded interdisciplinary projects, working with mathematical modelers and physicists to bridge nuclear shape and cytoskeleton dynamics. Research Publications I have authored or co-authored 20 research articles and 6 book chapters, published in high-impact journals such as Nature Plants, The Plant Journal, and Journal of Experimental Botany. Notable works include: Scholarships and Recognition I have been honored with several awards, including the Overseas Postdoctoral Fellowship by SERB-DST, India (2017, $36,000), CSIR-SRF (2014), and qualifications in CSIR-NET (2012) and GATE (2011). I have presented my research at national and international conferences, including talks at the Biophysique et biomécanique des plantes conference (2022) and posters at the Plant Morphodynamics conference (2019). Outreach and Networking I actively engage in scientific outreach via social media platforms like Twitter (@g_singhbt), LinkedIn, ResearchGate, and Google Scholar, where my work is widely accessible. I collaborate with experts globally and maintain strong professional references. My career reflects a deep commitment to advancing plant science through innovative research, technical expertise, and collaborative efforts, positioning me as a versatile and impactful researcher in the field.

Subject Matter Expertise

• ☆ Botany
• ☆ Plant Physiology
• ☆ Medicinal Plants
• ☆ Cell Biology
• ☆ Gene Regulation
• ☆ Biotechnology & Bioengineering
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Electron Microscopy
• ☆ Agronomy & Crop Science
• ☆ Plant Biotechnology
• ☆ Environmental Science
• ☆ Environmental Biotechnology
• ☆ Agricultural Biotechnology
• ☆ Tissue Engineering

Services

• Writing
• Research
• Consulting
• Data & AI

Writing Technical Writing

Research Scientific and Technical Research

Consulting Scientific and Technical Consulting

Data & AI Image Analysis

Work Experience

CEA Cadarache

December 2023 - Present

Postdoctoral fellow

CEA Cadarache

June 2022 - August 2023

Postdoctoral fellow

Institut de Biologie Moléculaire des Plantes

December 2018 - May 2022

Visiting Academic

University of Warwick

December 2017 - January 2019

Project JRF

National Botanical Research Institute CSIR

April 2017 - November 2017

Education

PhD biotechnology

Banaras Hindu University

September 2012 - January 2017

PhD (Department of Botany)

Banaras Hindu University

2013 - 2016

Master biotechnology

Punjab Technical University

September 2008 - December 2010

BSc Biology

Dr. Ram Manohar Lohia Avadh University - India

July 2005 - December 2008

Certifications

• Master training

  CSIR-NBRI INDIA

  May 2010 - November 2010

Publications

JOURNAL ARTICLE

Gaurav Singh, Shey‐Li Lim, Jinhong Liu, Gilles Dupouy, St&#233;phanie Baudrey, Lang Yang, Jia Yi Zhong, Marie-Edith Chabout&#233;, Lim, BL (2024). <i>In planta</i> imaging of pyridine nucleotides using second‐generation fluorescent protein biosensors . The Plant Journal.

Gaurav Singh, Jaspreet Kaur, Gaurav Mudgal, Kartar Chand, Gajendra B. Singh, Kahkashan Perveen, Najat A. Bukhari, Sandip Debnath, Thotegowdanapalya C. Mohan, Rajulu Charukesi (2022). An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention . Scientific Reports.

Gaurav Singh, Morgane Batzenschlager, Denisa Tomkova, Etienne Herzog, Elise Hoffmann, Guy Houln&#233;, Anne-Catherine Schmit, Alexandre Berr, Marie-Edith Chabout&#233; (2022). GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery . Frontiers in Plant Science.

Gaurav Singh, David Pereira, St&#233;phanie Baudrey, Elise Hoffmann, Ryckelynck, Atef Asnacios, Marie-Edith Chabout&#233; (2021). Real‐time tracking of root hair nucleus morphodynamics using a microfluidic approach . The Plant Journal.

(2018). Advances in Understanding the Mechanism of Action of the Auxin Permease AUX1 . International journal of molecular sciences.

(2018). Elicitation Enhanced the Yield of Glycyrrhizin and Antioxidant Activities in Hairy Root Cultures of <i>Glycyrrhiza glabra</i> L . Journal of plant growth regulation.

(2018). It starts with TIRs . Nature plants.

(2017). Exploring the functional significance of sterol glycosyltransferase enzymes . Progress in lipid research.

Gaurav Singh, Manish Tiwari, Surendra P. Singh, Ruchi Singh, Surendra Singh, Pramod A. Shirke, Prabodh K. Trivedi, Pratibha Misra(2017). Sterol glycosyltransferases required for adaptation of Withania somnifera at high temperature . Physiologia Plantarum. 160. (3). p. 297--311. Wiley

Manoj K. Mishra and M. Srivastava and G. Singh and S. Tiwari and A. Niranjan and N. Kumari and P. Misra(2017). Overexpression of Withania somnifera SGTL1 gene resists the interaction of fungus Alternaria brassicicola in Arabidopsis thaliana . Physiological and Molecular Plant Pathology. 97. p. 11--19. Elsevier {BV}

(2016). Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera . Scientific reports.

(2015). Characterization of Arabidopsis sterol glycosyltransferase TTG15/UGT80B1 role during freeze and heat stress . Plant signaling & behavior.

(2013). Overexpression of WsSGTL1 gene of Withania somnifera enhances salt tolerance, heat tolerance and cold acclimation ability in transgenic Arabidopsis plants . PloS one.

Dibya D. Toppo and Gaurav Singh and D.K. Purshottam and Pratibha Misra(2012). Improved in vitro rooting and acclimatization of Jatropha curcas plantlets . Biomass and Bioenergy. 44. p. 42--46. Elsevier {BV}

Pratibha Misra and Dibya Dolridge Toppo and Manoj Kumar Mishra and Syed Saema and Gaurav Singh(2012). Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments . Journal of Plant Biochemistry and Biotechnology. 21. (1). p. 128--133. Springer Science and Business Media {LLC}

BOOK CHAPTER

Gaurav Singh (2023). Studies of Natural Product Synthesis of Withania somnifera and Their Conservation Strategy Through In Vitro Method . Plants for Immunity and Conservation Strategies.

Gaurav Singh, David Pereira, Thomas Alline, Marie-Edith Chabout&#233;, Atef Asnacios (2023). A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity Sensing by Plant’s Roots and Root Hair Cells .

Gaurav Singh and Mrinalini Srivastava and Pratibha Misra(2016). Genetic Transformation for Quality Improvement in Ornamental Climbers . Biotechnological strategies for the conservation of medicinal and ornamental climbers. p. 351--365. Springer International Publishing

Mrinalini Srivastava and Gaurav Singh and Pratibha Misra(2016). Contribution of Biotechnological Tools in the Enhancement of Secondary Metabolites in Selected Medicinal Climbers . Biotechnological strategies for the conservation of medicinal and ornamental climbers. p. 465--486. Springer International Publishing

PREPRINT

Yihan Dong and Veli V. Uslu and Alexandre Berr and Gaurav Singh and Csaba Papdi and Victor A Steffens and Thierry Heitz and Lyubov Ryabova(2021). TOR represses stress responses through global regulation of H3K27 trimethylation in plants . []. Cold Spring Harbor Laboratory