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USD 380 /hr
Hire Douglas G.
United States
USD 380 /hr

Global Virology Leader | VP Veterinary Pharma Seek Labs | CRISPR‑based Therapeutics | African Swine Fever Vaccine Expert

Profile Summary
Subject Matter Expertise
Services
Writing Technical Writing
Research Market Research, Meta-Research, Feasibility Study, Gap Analysis, Gray Literature Search, Scientific and Technical Research, Systematic Literature Review
Consulting Business Strategy Consulting, Financial Consulting, Regulatory Consulting, Manufacturing Consulting
Data & AI Image Analysis, Algorithm Design-Non ML, Data Visualization, Big Data Analytics, Data Mining, Data Processing
Product Development Recipe Development, Product Evaluation, Product Validation, Product Compliance , Concept Development, Reverse Engineering
Work Experience

Director of Genomics Center

Global Alliance for African swine fever

October 2024 - Present

Vice President Veterinary Pharmaceuticals

Seek Labs

June 2024 - Present

Senior Research Scientist

USDA Agricultural Research Service

May 2015 - June 2024

Research Associate

University of Connecticut

2010 - 2015

Research Microbiologist

USDA Agricultural Research Service

2007 - 2010

Graduate Researcher

Stony Brook University

2001 - 2007

Summer Internship

Alexion Pharmaceuticals

2000 - 2000

Summer Internship

Pfizer Global Research and Development

1998 - 1998

Summer Intern

Pfizer Global Research and Development

1998 - 1998

Education

Ph.D. Molecular Genetics and Microbiology (Molecular Genetics and Microbiology)

Stony Brook University

2001 - 2007

B.S. Microbiologoy (Microbioogy)

University of Rhode Island

1997 - 2001

Certifications

  • Public Trust Select Agent Clearance

    USDA

    January 2020 - January 2025

Publications
JOURNAL ARTICLE
Manuel V. Borca, Elizabeth Ramirez-Medina, Christine Mutisya, Rose Ojuok, Josiah Odaba, Mark Dihbol, Anna Lacasta, Douglas P. Gladue (2025). Evaluation of Cross-Protection of African Swine Fever Vaccine ASFV-G-ΔI177L Between ASFV Biotypes . Vaccines.
Manuel V. Borca, Elizabeth Ramirez-Medina, Christine Mutisya, Rose Ojuok, Josiah Odaba, Mark Dihbol, Anna Lacasta, Douglas P. Gladue (2025). Evaluation of Cross-Protection of African Swine Fever Vaccine ASFV-G-ΔI177L Between ASFV Biotypes . Vaccines.
Edward Spinard, Vivian K. O'Donnell, Ayushi Rai, Amanda Meyers, Allyssa Valladares, Elizabeth Ramirez-Medina, Bonto Faburay, Manuel V. Borca, Douglas P. Gladue, Kenneth M. Stedman (2025). Near complete genome sequence of the historic African swine fever isolate Uganda-898 . Microbiology Resource Announcements.
Mark Dinhobl, Edward Spinard, Hillary Birtlery, Nicolas Tesler, Charles Masembe, Paolo Ribeca, Manuel V. Borca, Douglas P. Gladue, Simon Roux (2025). African swine fever virus biotype identification tool . Microbiology Resource Announcements.
Elizabeth Ramirez-Medina, Lauro Velazquez-Salinas, Alyssa Valladares, Amanda Meyers, Leeanna Burton, Ediane Silva, Jason Clark, Manuel V. Borca, Douglas P. Gladue (2024). Evaluation of the Deletion of African Swine Fever Virus E111R Gene from the Georgia Isolate in Virus Replication and Virulence in Domestic Pigs . Viruses.
Elizabeth Ramirez-Medina, Lauro Velazquez-Salinas, Alyssa Valladares, Amanda Meyers, Leeanna Burton, Ediane Silva, Jason Clark, Manuel V. Borca, Douglas P. Gladue (2024). Evaluation of the Deletion of African Swine Fever Virus E111R Gene from the Georgia Isolate in Virus Replication and Virulence in Domestic Pigs . Viruses.
Douglas P. Gladue, Lidia Gomez-Lucas, Eneko Largo, Elizabeth Ramirez-Medina, Johana Torralba, Maria Queralt-Martín, Antonio Alcaraz, Lauro Velazquez-Salinas, Jose L. Nieva, Manuel V. Borca, et al. (2024). Viroporin-like activity of the hairpin transmembrane domain of African swine fever virus B169L protein . Journal of Virology.
Ayushi Rai, Edward Spinard, Jehadi Osei-Bonsu, Amanda Meyers, Mark Dinhobl, Vivian O’Donnell, Patrick T. Ababio, Daniel Tawiah-Yingar, Daniel Arthur, Daniel Baah, et al. (2024). A Retrospective Analysis Reveals That the 2021 Outbreaks of African Swine Fever Virus in Ghana Were Caused by Two Distinct Genotypes . Viruses.
Liani Coronado, Adriana Muñoz-Aguilera, Guillermo Cantero, Patricia Martínez, Mònica Alberch, Rosa Rosell, Douglas P. Gladue, Manuel V. Borca, Llilianne Ganges (2024). FlagT4G Vaccine Prevents Transplacental Transmission of Highly Virulent Classical Swine Fever Virus after Single Vaccination in Pregnant Sows . Vaccines.
Liani Coronado, Adriana Muñoz-Aguilera, Guillermo Cantero, Patricia Martínez, Mònica Alberch, Rosa Rosell, Douglas P. Gladue, Manuel V. Borca, Llilianne Ganges (2024). FlagT4G Vaccine Prevents Transplacental Transmission of Highly Virulent Classical Swine Fever Virus after Single Vaccination in Pregnant Sows . Vaccines.
Edward Spinard, Mark Dinhobl, Jacob Fenster, Charronne Davis, Manuel V. Borca, Douglas P. Gladue (2024). Analysis of the Unique Historical Isolate of African Swine Fever Virus Isolate Spencer from Outbreaks in 1951 . Viruses.
Vivian O’Donnell, Jim L. Pierce, Oleg Osipenko, Lizhe Xu, Amy Berninger, Steven M. Lakin, Roger W. Barrette, Douglas P. Gladue, Bonto Faburay (2024). Rapid Detection and Quick Characterization of African Swine Fever Virus Using the VolTRAX Automated Library Preparation Platform . Viruses.
Vivian O’Donnell, Jim L. Pierce, Oleg Osipenko, Lizhe Xu, Amy Berninger, Steven M. Lakin, Roger W. Barrette, Douglas P. Gladue, Bonto Faburay (2024). Rapid Detection and Quick Characterization of African Swine Fever Virus Using the VolTRAX Automated Library Preparation Platform . Viruses.
Edward Spinard, Abel Wade, Hermann Unger, Nenkam Robert, Francis J. Mayega, Vattipally B. Sreenu, Ana Da Silva Filpe, Daniel Mair, Manuel V. Borca, Douglas P. Gladue, et al. (2024). Near-complete genome sequences of multiple genotype 1 African swine fever virus isolates from 2016 to 2018 in Cameroon . Microbiology Resource Announcements.
Elizabeth Ramirez-Medina, Ayushi Rai, Nallely Espinoza, Edward Spinard, Ediane Silva, Leeanna Burton, Jason Clark, Amanda Meyers, Alyssa Valladares, Lauro Velazquez-Salinas, et al. (2024). Recombinant Vaccine Strain ASFV-G-Δ9GL/ΔUK Produced in the IPKM Cell Line Is Genetically Stable and Efficacious in Inducing Protection in Pigs Challenged with the Virulent African Swine Fever Virus Field Isolate Georgia 2010 . Pathogens.
Mark Dinhobl, Edward Spinard, Hillary Birtley, Nicolas Tesler, Manuel V. Borca, Douglas P. Gladue, Simon Roux (2024). African swine fever virus P72 genotyping tool . Microbiology Resource Announcements.
Manuel V. Borca, Elizabeth Ramirez-Medina, Nallely Espinoza, Ayushi Rai, Edward Spinard, Lauro Velazquez-Salinas, Alyssa Valladares, Ediane Silva, Leeanna Burton, Amanda Meyers, et al. (2024). Deletion of the EP402R Gene from the Genome of African Swine Fever Vaccine Strain ASFV-G-∆I177L Provides the Potential Capability of Differentiating between Infected and Vaccinated Animals . Viruses.
Abdel-Moneim, Ahmed S., Murovska, Modra, Soderlund-Venermo, Maria, Vakharia, Vikram N., Wilson, William C., Gladue, Douglas P., Moore, Matthew D., Alonso, Covadonga, Abdelwahab, Sayed F., Venter, Marietjie, et al. (2024). WSV2023-The second meeting of the world society for virology: One health - One world - One virology . Virology.
Mark Dinhobl, Edward Spinard, Nicolas Tesler, Hillary Birtley, Anthony Signore, Aruna Ambagala, Charles Masembe, Manuel V. Borca, Douglas P. Gladue (2023). Reclassification of ASFV into 7 Biotypes Using Unsupervised Machine Learning . Viruses.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Ediane Silva, Keith Berggren, Ayushi Rai, Nallely Espinoza, Manuel V. Borca, Douglas P. Gladue (2023). The Interaction between the DOCK7 Protein and the E2 Protein of  Classical Swine Fever Virus Is Not Involved with Viral Replication or Pathogenicity . Viruses.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Ediane Silva, Keith Berggren, Ayushi Rai, Nallely Espinoza, Manuel V. Borca, Douglas P. Gladue (2023). The Interaction between the DOCK7 Protein and the E2 Protein of  Classical Swine Fever Virus Is Not Involved with Viral Replication or Pathogenicity . Viruses.
Mark Dinhobl, Edward Spinard, Nicolas Tesler, Hillary Birtley, Anthony Signore, Aruna Ambagala, Charles Masembe, Manuel V. Borca, Douglas P. Gladue (2023). Reclassification of ASFV into 7 Biotypes Using Unsupervised Machine Learning . Viruses.
Edward Spinard, Mark Dinhobl, Nicolas Tesler, Hillary Birtley, Anthony V. Signore, Aruna Ambagala, Charles Masembe, Manuel V. Borca, Douglas P. Gladue (2023). A Re-Evaluation of African Swine Fever Genotypes Based on p72 Sequences Reveals the Existence of Only Six Distinct p72 Groups . Viruses.
Edward Spinard, Mark Dinhobl, Nicolas Tesler, Hillary Birtley, Anthony V. Signore, Aruna Ambagala, Charles Masembe, Manuel V. Borca, Douglas P. Gladue (2023). A Re-Evaluation of African Swine Fever Genotypes Based on p72 Sequences Reveals the Existence of Only Six Distinct p72 Groups . Viruses.
Ediane Silva, Elizabeth Medina-Ramirez, Selvaraj Pavulraj, Douglas P. Gladue, Manuel Borca, Shafiqul I. Chowdhury (2023). A Triple Gene-Deleted Pseudorabies Virus-Vectored Subunit PCV2b and CSFV Vaccine Protect Pigs against a Virulent CSFV Challenge . Viruses.
Ediane Silva, Elizabeth Medina-Ramirez, Selvaraj Pavulraj, Douglas P. Gladue, Manuel Borca, Shafiqul I. Chowdhury (2023). A Triple Gene-Deleted Pseudorabies Virus-Vectored Subunit PCV2b and CSFV Vaccine Protect Pigs against a Virulent CSFV Challenge . Viruses.
Manuel V. Borca, Ayushi Rai, Nallely Espinoza, Elizabeth Ramirez-Medina, Edward Spinard, Lauro Velazquez-Salinas, Alyssa Valladares, Ediane Silva, Leeanna Burton, Amanda Meyers, et al. (2023). African Swine Fever Vaccine Candidate ASFV-G-ΔI177L Produced in the Swine Macrophage-Derived Cell Line IPKM Remains Genetically Stable and Protective against Homologous Virulent Challenge . Viruses.
Elizabeth Ramirez-Medina, Lauro Velazquez-Salinas, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Ediane Silva, Leeanna Burton, Edward Spinard, Amanda Meyers, Guillermo Risatti, et al. (2023). Evaluation of the Deletion of the African Swine Fever Virus Gene <i>O174L</i> from the Genome of the Georgia Isolate . Viruses.
Edward Spinard, Ayushi Rai, Jehadi Osei-Bonsu, Vivian O’Donnell, Patrick T. Ababio, Daniel Tawiah-Yingar, Daniel Arthur, Daniel Baah, Elizabeth Ramirez-Medina, Nallely Espinoza, et al. (2023). The 2022 Outbreaks of African Swine Fever Virus Demonstrate the First Report of Genotype II in Ghana . Viruses.
Douglas P. Gladue, Lidia Gomez-Lucas, Eneko Largo, Lauro Velazquez-Salinas, Elizabeth Ramirez-Medina, Johana Torralba, Maria Queralt, Antonio Alcaraz, Jose L. Nieva, Manuel V. Borca, et al. (2023). African Swine Fever Virus Gene B117L Encodes a Small Protein Endowed with Low-pH-Dependent Membrane Permeabilizing Activity . Journal of Virology.
Elizabeth Ramirez-Medina, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2023). Deletion of the H240R Gene in African Swine Fever Virus Partially Reduces Virus Virulence in Swine . Viruses.
Manuel V. Borca, Elizabeth Ramirez-Medina, Ediane Silva, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue (2023). ASF Vaccine Candidate ASFV-G-∆I177L Does Not Exhibit Residual Virulence in Long-Term Clinical Studies . Pathogens.
Manuel V. Borca, Elizabeth Ramirez-Medina, Ediane Silva, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue (2023). ASF Vaccine Candidate ASFV-G-∆I177L Does Not Exhibit Residual Virulence in Long-Term Clinical Studies . Pathogens.
Elizabeth Ramirez-Medina, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2023). Deletion of the <i>H240R</i> Gene in African Swine Fever Virus Partially Reduces Virus Virulence in Swine . Viruses.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Ediane Silva, Keith Berggren, Ayushi Rai, Nallely Espinoza, Douglas P. Gladue, Manuel V. Borca (2023). Classical Swine Fever Virus Structural Glycoprotein E2 Interacts with Host Protein ACADM during the Virus Infectious Cycle . Viruses.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Ediane Silva, Keith Berggren, Ayushi Rai, Nallely Espinoza, Douglas P. Gladue, Manuel V. Borca (2023). Classical Swine Fever Virus Structural Glycoprotein E2 Interacts with Host Protein ACADM during the Virus Infectious Cycle . Viruses.
Aruna Ambagala, Kalhari Goonewardene, Lindsey Lamboo, Melissa Goolia, Cassidy Erdelyan, Mathew Fisher, Katherine Handel, Oliver Lung, Sandra Blome, Jacqueline King, et al. (2023). Characterization of a Novel African Swine Fever Virus p72 Genotype II from Nigeria . Viruses.
Lihua Wang, Rachel Madera, Yuzhen Li, Douglas P. Gladue, Manuel V. Borca, Michael T. McIntosh, Jishu Shi (2023). Development of Porcine Monoclonal Antibodies with In Vitro Neutralizing Activity against Classical Swine Fever Virus from C-Strain E2-Specific Single B Cells . Viruses.
Lihua Wang, Rachel Madera, Yuzhen Li, Douglas P. Gladue, Manuel V. Borca, Michael T. McIntosh, Jishu Shi (2023). Development of Porcine Monoclonal Antibodies with In Vitro Neutralizing Activity against Classical Swine Fever Virus from C-Strain E2-Specific Single B Cells . Viruses.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2023). Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine . Viruses.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2023). Evaluation of the Function of ASFV Gene E66L in the Process of Virus Replication and Virulence in Swine . Viruses.
Spinard, Edward, O&#39;Donnell, Vivian, Vuono, Elizabeth, Rai, Ayushi, Davis, Charronne, Ramirez-Medina, Elizabeth, Espinoza, Nallely, Valladares, Alyssa, Borca, Manuel V., Gladue, Douglas P. P. (2023). Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980 . Scientific Reports.
Velazquez-Salinas, Lauro, Ramirez-Medina, Elizabeth, Rai, Ayushi, Pruitt, Sarah, Vuono, Elizabeth A., Espinoza, Nallely, Gay, Cyril G., Witte, Steve, Gladue, Douglas P., V. Borca, Manuel (2023). Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines . Biologicals.
Hyeon, Ji-Yeon, Tseren-Ochir, Erdene-Ochir, Lee, Dong-Hun, Nahm, Sang-Soep, Gladue, Douglas P. P., Borca, Manuel V. V., Song, Chang-Seon, Risatti, Guillermo R. R. (2023). Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019 . Frontiers in Veterinary Science.
Edward Spinard, Paul Azzinaro, Ayushi Rai, Nallely Espinoza, Elizabeth Ramirez-Medina, Alyssa Valladares, Manuel V. Borca, Douglas P. Gladue, Kenneth M. Stedman (2022). Complete Structural Predictions of the Proteome of African Swine Fever Virus Strain Georgia 2007 . Microbiology Resource Announcements.
A Highly Effective African Swine Fever Virus Vaccine Elicits a Memory T Cell Response in Vaccinated Swine <head> <META HTTP-EQUIV="Refresh" CONTENT="0;URL=/servlet/useragent"> </head> . Pathogens.
The Presence of Virus Neutralizing Antibodies Is Highly Associated with Protection against Virulent Challenge in Domestic Pigs Immunized with ASFV live Attenuated Vaccine Candidates <head> <META HTTP-EQUIV="Refresh" CONTENT="0;URL=/servlet/useragent"> </head> . Pathogens.
Elizabeth Ramirez-Medina, Elisabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Edward Spinard, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, et al. (2022). Deletion of an African Swine Fever Virus ATP-Dependent RNA Helicase <i>QP509L</i> from the Highly Virulent Georgia 2010 Strain Does Not Affect Replication or Virulence . Viruses.
Ediane B. Silva, Peter W. Krug, Elizabeth Ramirez-Medina, Alyssa Valladares, Ayushi Rai, Nallely Espinoza, Douglas P. Gladue, Manuel V. Borca (2022). The Presence of Virus Neutralizing Antibodies Is Highly Associated with Protection against Virulent Challenge in Domestic Pigs Immunized with ASFV live Attenuated Vaccine Candidates . Pathogens.
Sarah E. Attreed, Christina Silva, Sophia Abbott, Elizabeth Ramirez-Medina, Nallely Espinoza, Manuel V. Borca, Douglas P. Gladue, Fayna Diaz-San Segundo (2022). A Highly Effective African Swine Fever Virus Vaccine Elicits a Memory T Cell Response in Vaccinated Swine . Pathogens.
Jos&#233; Alejandro Boh&#243;rquez, Miaomiao Wang, Ivan D&#237;az, M&#242;nica Alberch, Marta P&#233;rez-Sim&#243;, Rosa Rosell, Douglas P. Gladue, Manuel V. Borca, Llilianne Ganges (2022). The FlagT4G Vaccine Confers a Strong and Regulated Immunity and Early Virological Protection against Classical Swine Fever . Viruses.
Jos&#233; Alejandro Boh&#243;rquez, Miaomiao Wang, Ivan D&#237;az, M&#242;nica Alberch, Marta P&#233;rez-Sim&#243;, Rosa Rosell, Douglas P. Gladue, Manuel V. Borca, Llilianne Ganges (2022). The FlagT4G Vaccine Confers a Strong and Regulated Immunity and Early Virological Protection against Classical Swine Fever . Viruses.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, et al. (2022). ASFV Gene A151R Is Involved in the Process of Virulence in Domestic Swine . Viruses.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Edward Spinard, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, et al. (2022). ASFV Gene A151R Is Involved in the Process of Virulence in Domestic Swine . Viruses.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Ediane Silva, Ayushi Rai, Sarah Pruitt, Nallely Espinoza, Alyssa Valladares, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca, et al. (2022). Deletion of the H108R Gene Reduces Virulence of the Pandemic Eurasia Strain of African Swine Fever Virus with Surviving Animals Being Protected against Virulent Challenge . Journal of Virology.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Ediane Silva, Ayushi Rai, Alyssa Valladares, Sarah Pruitt, Nallely Espinoza, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue, et al. (2022). Evaluation of the Deletion of MGF110-5L-6L on Swine Virulence from the Pandemic Strain of African Swine Fever Virus and Use as a DIVA Marker in Vaccine Candidate ASFV-G-ΔI177L . Journal of Virology.
Xuan Hanh Tran, Thi Thu Phuong Le, Quang Huy Nguyen, Thanh Thuy Do, Van Dung Nguyen, Cyril G. Gay, Manuel V. Borca, Douglas P. Gladue (2022). African swine fever virus vaccine candidate ASFV‐G‐ΔI177L efficiently protects European and native pig breeds against circulating Vietnamese field strain . Transboundary and Emerging Diseases.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Edward Spinard, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, et al. (2022). Deletion of the EP296R Gene from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs . Viruses.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2022). Deletion of the ASFV dUTPase Gene E165R from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs . Viruses.
Jessica A. Canter, Theresa Aponte, Elizabeth Ramirez-Medina, Sarah Pruitt, Douglas P. Gladue, Manuel V. Borca, James J. Zhu (2022). Serum Neutralizing and Enhancing Effects on African Swine Fever Virus Infectivity in Adherent Pig PBMC . Viruses.
Jessica A. Canter, Theresa Aponte, Elizabeth Ramirez-Medina, Sarah Pruitt, Douglas P. Gladue, Manuel V. Borca, James J. Zhu (2022). Serum Neutralizing and Enhancing Effects on African Swine Fever Virus Infectivity in Adherent Pig PBMC . Viruses.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2022). Deletion of the ASFV dUTPase Gene <i>E165R</i> from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs . Viruses.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2022). Deletion of African Swine Fever Virus Histone-like Protein, A104R from the Georgia Isolate Drastically Reduces Virus Virulence in Domestic Pigs . Viruses.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2022). Deletion of African Swine Fever Virus Histone-like Protein, A104R from the Georgia Isolate Drastically Reduces Virus Virulence in Domestic Pigs . Viruses.
Elizabeth Ramirez-Medina, Vivian O’Donnell, Ediane Silva, Nallely Espinoza, Lauro Velazquez-Salinas, Karen Moran, Dee Ann Daite, Roger Barrette, Bonto Faburay, Robin Holland, et al. (2022). Experimental Infection of Domestic Pigs with an African Swine Fever Virus Field Strain Isolated in 2021 from the Dominican Republic . Viruses.
Xuan Hanh Tran, Le Thi Thu Phuong, Nguyen Quang Huy, Do Thanh Thuy, Van Dung Nguyen, Pham H&#224;o Quang, Qu&#225;ch V&#245; Ng&#244;n, Ayushi Rai, Cyril G. Gay, Douglas Paul Gladue, et al.(2022). Evaluation of the Safety Profile of the ASFV Vaccine Candidate ASFV-G-ΔI177L . Viruses. 14. (5). p. 896. {MDPI} {AG}
Douglas P. Gladue, Manuel V. Borca (2022). Recombinant ASF Live Attenuated Virus Strains as Experimental Vaccine Candidates . Viruses.
Douglas P. Gladue, Manuel V. Borca (2022). Recombinant ASF Live Attenuated Virus Strains as Experimental Vaccine Candidates . Viruses.
Xuan Hanh Tran, Le Thi Thu Phuong, Nguyen Quang Huy, Do Thanh Thuy, Van Dung Nguyen, Pham H&#224;o Quang, Qu&#225;ch V&#245; Ng&#244;n, Ayushi Rai, Cyril G. Gay, Douglas Paul Gladue, et al. (2022). Evaluation of the Safety Profile of the ASFV Vaccine Candidate ASFV-G-ΔI177L . Viruses.
Ramirez-Medina, Elizabeth, Vuono, Elizabeth, Rai, Ayushi, Pruitt, Sarah, Espinoza, Nallely, Velazquez-Salinas, Lauro, Pina-Pedrero, Sonia, Zhu, James, Rodriguez, Fernando, Borca, Manuel, V, et al. (2022). Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge . Journal of Virology.
Soderlund-Venermo, Maria, Varma, Anupam, Guo, Deyin, Gladue, Douglas P., Poole, Emma, Pujol, Flor H., Pappu, Hanu, Romalde, Jesus L., Kramer, Laura, Baz, Mariana, et al. (2022). World Society for Virology first international conference: Tackling global virus epidemics . Virology.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2021). Evaluation of an ASFV RNA Helicase Gene A859L for Virus Replication and Swine Virulence . Viruses.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2021). Evaluation of an ASFV RNA Helicase Gene A859L for Virus Replication and Swine Virulence . Viruses.
Douglas P. Gladue, Elizabeth Ramirez-Medina, Elizabeth Vuono, Ediane Silva, Ayushi Rai, Sarah Pruitt, Nallely Espinoza, Lauro Velazquez-Salinas, Manuel V. Borca, Joanna L. Shisler(2021). Deletion of the A137R Gene from the Pandemic Strain of African Swine Fever Virus Attenuates the Strain and Offers Protection against the Virulent Pandemic Virus . Journal of Virology. 95. (21). American Society for Microbiology
Jos&#233; Alejandro Boh&#243;rquez, Sira Defaus, Rosa Rosell, Marta P&#233;rez-Sim&#243;, M&#242;nica Alberch, Douglas P. Gladue, Manuel V. Borca, David Andreu, Llilianne Ganges (2021). Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs . Viruses.
Jos&#233; Alejandro Boh&#243;rquez, Sira Defaus, Rosa Rosell, Marta P&#233;rez-Sim&#243;, M&#242;nica Alberch, Douglas P. Gladue, Manuel V. Borca, David Andreu, Llilianne Ganges (2021). Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs . Viruses.
Elisabeth Lopez, Laia Bosch-Cam&#243;s, Elizabeth Ramirez-Medina, Elizabeth Vuono, Maria Jesus Navas, Marta Mu&#241;oz, Francesc Accensi, Jinya Zhang, Uxia Alonso, Jordi Argilaguet, et al. (2021). Deletion Mutants of the Attenuated Recombinant ASF Virus, BA71ΔCD2, Show Decreased Vaccine Efficacy . Viruses.
M. V. Borca, A. Rai, E. Ramirez-Medina, E. Silva, L. Velazquez-Salinas, E. Vuono, S. Pruitt, N. Espinoza, D. P. Gladue, Jae U. Jung(2021). A Cell Culture-Adapted Vaccine Virus against the Current African Swine Fever Virus Pandemic Strain . Journal of Virology. 95. (14). American Society for Microbiology
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2021). Evaluation of the Function of the ASFV KP177R Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence . Viruses.
E. A. Vuono, E. Ramirez-Medina, L. Velazquez-Salinas, K. Berggren, A. Rai, S. Pruitt, N. Espinoza, D. P. Gladue, M. V. Borca, Susana L&#243;pez (2021). Structural Glycoprotein E2 of Classical Swine Fever Virus Critically Interacts with Host Protein Torsin-1A during the Virus Infectious Cycle . Journal of Virology.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2021). Evaluation of the Function of the ASFV <i>KP177R</i> Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence . Viruses.
Manuel V. Borca, Elizabeth Ramirez-Medina, Ediane Silva, Elizabeth Vuono, Ayushi Rai, Sarah Pruitt, Nallely Espinoza, Lauro Velazquez-Salinas, Cyril G. Gay, Douglas P. Gladue (2021). ASFV-G-∆I177L as an Effective Oral Nasal Vaccine against the Eurasia Strain of Africa Swine Fever . Viruses.
Manuel V. Borca, Elizabeth Ramirez-Medina, Ediane Silva, Elizabeth Vuono, Ayushi Rai, Sarah Pruitt, Nallely Espinoza, Lauro Velazquez-Salinas, Cyril G. Gay, Douglas P. Gladue (2021). ASFV-G-∆I177L as an Effective Oral Nasal Vaccine against the Eurasia Strain of Africa Swine Fever . Viruses.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Ediane Silva, Nallely Espinoza, James Zhu, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2021). Development and In Vivo Evaluation of a MGF110-1L Deletion Mutant in African Swine Fever Strain Georgia . Viruses.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Ediane Silva, Nallely Espinoza, James Zhu, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2021). Development and In Vivo Evaluation of a MGF110-1L Deletion Mutant in African Swine Fever Strain Georgia . Viruses.
Rai, Ayushi, Pruitt, Sarah, Ramirez-Medina, Elizabeth, Vuono, Elizabeth A., Silva, Ediane, Velazquez-Salinas, Lauro, Carrillo, Consuelo, Borca, Manuel, V, Gladue, Douglas P. (2021). Detection and Quantification of African Swine Fever Virus in MA-104 Cells . Bio-Protocol.
Velazquez-Salinas, Lauro, Ramirez-Medina, Elizabeth, Rai, Ayushi, Pruitt, Sarah, Vuono, Elizabeth A., Espinoza, Nallely, Gladue, Douglas P., Borca, Manuel V. (2021). Development Real-Time PCR Assays to Genetically Differentiate Vaccinated Pigs From Infected Pigs With the Eurasian Strain of African Swine Fever Virus . Frontiers in Veterinary Science.
Lopera-Madrid, Jaime, Medina-Magues, Lex G., Gladue, Douglas P., Borca, Manuel V., Osorio, Jorge E. (2021). Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors . Scientific Reports.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Ediane Silva, Nallely Espinoza, James Zhu, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2020). Evaluation in Swine of a Recombinant Georgia 2010 African Swine Fever Virus Lacking the I8L Gene . Viruses.
Elizabeth Vuono, Elizabeth Ramirez-Medina, Sarah Pruitt, Ayushi Rai, Ediane Silva, Nallely Espinoza, James Zhu, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2020). Evaluation in Swine of a Recombinant Georgia 2010 African Swine Fever Virus Lacking the I8L Gene . Viruses.
Douglas P. Gladue, Vivian O’Donnell, Elizabeth Ramirez-Medina, Ayushi Rai, Sarah Pruitt, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca (2020). Deletion of CD2-Like (CD2v) and C-Type Lectin-Like (EP153R) Genes from African Swine Fever Virus Georgia-∆9GL Abrogates Its Effectiveness as an Experimental Vaccine . Viruses.
Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, James Zhu, Douglas P. Gladue, Manuel V. Borca (2020). Evaluation in Swine of a Recombinant African Swine Fever Virus Lacking the MGF-360-1L Gene . Viruses.
Douglas P. Gladue, Vivian O’Donnell, Elizabeth Ramirez-Medina, Ayushi Rai, Sarah Pruitt, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca (2020). Deletion of CD2-Like (CD2v) and C-Type Lectin-Like (EP153R) Genes from African Swine Fever Virus Georgia-∆9GL Abrogates Its Effectiveness as an Experimental Vaccine . Viruses.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Ediane Silva, James Zhu, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca(2020). X69R Is a Non-Essential Gene That, When Deleted from African Swine Fever, Does Not Affect Virulence in Swine . Viruses. 12. (9). p. 918. {MDPI} {AG}
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Ediane Silva, James Zhu, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2020). X69R Is a Non-Essential Gene That, When Deleted from African Swine Fever, Does Not Affect Virulence in Swine . Viruses.
Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel V. Borca, Douglas P. Gladue(2020). Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples . Viruses. 12. (8). p. 820. {MDPI} {AG}
Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel V. Borca, Douglas P. Gladue (2020). Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples . Viruses.
Elizabeth. Ramirez-Medina, Elizabeth. A. Vuono, Ayushi. Rai, Sarah. Pruitt, Ediane. Silva, Lauro. Velazquez-Salinas, James. Zhu, Manuel. V. Borca, Douglas. P. Gladue (2020). The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine . Viruses.
Elizabeth. Ramirez-Medina, Elizabeth. A. Vuono, Ayushi. Rai, Sarah. Pruitt, Ediane. Silva, Lauro. Velazquez-Salinas, James. Zhu, Manuel. V. Borca, Douglas. P. Gladue (2020). The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine . Viruses.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Paul Azzinaro, Keith A. Berggren, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue(2020). SERTA Domain Containing Protein 1 (SERTAD1) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2, Which Is Involved in Virus Virulence in Swine . Viruses. 12. (4). p. 421. {MDPI} {AG}
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Paul Azzinaro, Keith A. Berggren, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue (2020). SERTA Domain Containing Protein 1 (SERTAD1) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2, Which Is Involved in Virus Virulence in Swine . Viruses.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Keith Berggren, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca(2020). Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication . Viruses. 12. (4). p. 388. {MDPI} {AG}
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Keith Berggren, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, Manuel V. Borca (2020). Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication . Viruses.
Elizabeth Ram&#237;rez-Medina, Elizabeth A. Vuono, Lauro Velazquez-Salinas, Ediane Silva, Ayushi Rai, Sarah Pruitt, Keith A. Berggren, James Zhu, Manuel V. Borca, Douglas P. Gladue(2020). The MGF360-16R ORF of African Swine Fever Virus Strain Georgia Encodes for a Nonessential Gene That Interacts with Host Proteins SERTAD3 and SDCBP . Viruses. 12. (1). p. 60. {MDPI} {AG}
Elizabeth Ram&#237;rez-Medina, Elizabeth A. Vuono, Lauro Velazquez-Salinas, Ediane Silva, Ayushi Rai, Sarah Pruitt, Keith A. Berggren, James Zhu, Manuel V. Borca, Douglas P. Gladue (2020). The MGF360-16R ORF of African Swine Fever Virus Strain Georgia Encodes for a Nonessential Gene That Interacts with Host Proteins SERTAD3 and SDCBP . Viruses.
Velazquez-Salinas, Lauro, Pauszek, Steven J., Holinka, Lauren G., Gladue, Douglas P., Rekant, Steven I., Bishop, Elizabeth A., Stenfeldt, Carolina, Verdugo-Rodriguez, Antonio, Borca, Manuel V., Arzt, Jonathan, et al. (2020). A Single Amino Acid Substitution in the Matrix Protein (M51R) of Vesicular Stomatitis New Jersey Virus Impairs Replication in Cultured Porcine Macrophages and Results in Significant Attenuation in Pigs . Frontiers in Microbiology.
Borca, Manuel, V, O&#39;Donnell, Vivian, Holinka, Lauren G., Risatti, Guillermo R., Ramirez-Medina, Elizabeth, Vuono, Elizabeth A., Shi, Jishu, Pruitt, Sarah, Rai, Ayushi, Silva, Ediane, et al. (2020). Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine . Scientific Reports.
Borca, Manuel, V, Ramirez-Medina, Elizabeth, Silva, Ediane, Vuono, Elizabeth, Rai, Ayushi, Pruitt, Sarah, Holinka, Lauren G., Velazquez-Salinas, Lauro, Zhu, James, Gladue, Douglas P. (2020). Development of a Highly Effective African Swine Fever Virus Vaccine by Deletion of the I177L Gene Results in Sterile Immunity against the Current Epidemic Eurasia Strain . Journal of Virology.
Velazquez-Salinas, Lauro, Zarate, Selene, Eberl, Samantha, Gladue, Douglas P., Novella, Isabel, Borca, Manuel, V (2020). Positive Selection of ORF1ab, ORF3a, and ORF8 Genes Drives the Early Evolutionary Trends of SARS-CoV-2 During the 2020 COVID-19 Pandemic . Frontiers in Microbiology.
Kloc, Anna, Rai, Devendra K., Gladue, Douglas P., Schafer, Elizabeth, Kenney, Mary, Rieder, Elizabeth (2020). Residues within the Foot-and-Mouth Disease Virus 3D(pol) Nuclear Localization Signal Affect Polymerase Fidelity . Journal of Virology.
Borca, M. V., Vuono, E. A., Ramirez-Medina, E., Azzinaro, P., Berggren, K. A., Singer, M., Rai, A., Pruitt, S., Silva, E. B., Velazquez-Salinas, L., et al. (2020). Structural Glycoprotein E2 of Classical Swine Fever Virus Interacts with Host Protein Dynactin Subunit 6 (DCTN6) during the Virus Infectious Cycle . Journal of Virology.
James J. Zhu, Shuhong Zhao, Palaniappan Ramanathan, Elizabeth A. Bishop, Vivian O’Donnell, Douglas P. Gladue, Manuel V. Borca (2019). Mechanisms of African swine fever virus pathogenesis and immune evasion inferred from gene expression changes in infected swine macrophages . PLOS ONE.
Elizabeth A. Vuono, Elizabeth Ramirez-Medina, Lauren G. Holinka, Ryan Baker-Branstetter, Manuel V. Borca, Douglas P. Gladue (2019). Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB) . Viruses.
Gladue, D. P., O&#39;Donnell, V., Baker-Branstetter, R., Holinka, L. G., Pacheco, J. M., Sainz, I. Fernandez, Lu, Z., Ambroggio, X., Rodriguez, L., Borca, M. V. (2019). Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival (vol 87, pg 6794, 2013) . Journal of Virology.
Borca, M. V., Holinka, L. G., Ramirez-Medina, E., Risatti, G. R., Vuono, E. A., Berggren, K. A., Gladue, D. P. (2019). Identification of structural glycoprotein E2 domain critical to mediate replication of Classical Swine Fever Virus in SK6 cells . Virology.
Brown, Peter, Tan, Aik-Choon, El-Esawi, Mohamed A., Liehr, Thomas, Blanck, Oliver, Gladue, Douglas P., Almeida, Gabriel M. F., Cernava, Tomislav, Sorzano, Carlos O., Yeung, Andy W. K., et al. (2019). Large expert-curated database for benchmarking document similarity detection in biomedical literature search . Database.
Ramirez-Medina, Elizabeth, Vuono, Elizabeth, O&#39;Donnell, Vivian, Holinka, Lauren G., Silva, Ediane, Rai, Ayushi, Pruitt, Sarah, Carrillo, Consuelo, Gladue, Douglas P., Borca, Manuel, V (2019). Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain . Viruses.
Douglas P. Gladue, Eneko Largo, Lauren G. Holinka, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Keith A. Berggren, Guillermo R. Risatti, Jose L. Nieva, Manuel V. Borca (2018). Classical Swine Fever Virus p7 Protein Interacts with Host Protein CAMLG and Regulates Calcium Permeability at the Endoplasmic Reticulum . Viruses.
Douglas P. Gladue, Eneko Largo, Lauren G. Holinka, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Keith A. Berggren, Guillermo R. Risatti, Jose L. Nieva, Manuel V. Borca (2018). Classical Swine Fever Virus p7 Protein Interacts with Host Protein CAMLG and Regulates Calcium Permeability at the Endoplasmic Reticulum . Viruses.
Borca MV, O&#39;Donnell V, Holinka LG, Ram&#237;rez-Medina E, Clark BA, Vuono EA, Berggren K, Alfano M, Carey LB, Richt JA, et al.(2018). The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1β . Virus research.
Manuel V. Borca, Lauren G. Holinka, Keith A. Berggren, Douglas P. Gladue (2018). CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses . Scientific Reports.
Stenfeldt, C., Arzt, J., Pacheco, J.M., Gladue, D.P., Smoliga, G.R., Silva, E.B., Rodriguez, L.L., Borca, M.V.(2018). A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection . Virology. 516. p. 115-126.
Borca, Manuel, V, Berggren, Keith A., Ramirez-Medina, Elizabeth, Vuono, Elizabeth A., Gladue, Douglas P. (2018). CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus . Bio-Protocol.
Gladue, D. P., Largo, E., de la Arada, I, Aguilella, V. M., Alcaraz, A., Arrondo, J. L. R., Holinka, L. G., Brocchi, E., Ramirez-Medina, E., Vuono, E. A., et al. (2018). Molecular Characterization of the Viroporin Function of Foot- and-Mouth Disease Virus Nonstructural Protein 2B . Journal of Virology.
Largo, E., Gladue, D.P., Torralba, J., Aguilella, V.M., Alcaraz, A., Borca, M.V., Nieva, J.L.(2018). Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses . Biochimica et Biophysica Acta - Biomembranes. 1860. (5). p. 1015-1021.
Velazquez-Salinas, L., Ramirez-Medina, E., Bracht, A.J., Hole, K., Brito, B.P., Gladue, D.P., Carrillo, C.(2018). Phylodynamics of parapoxvirus genus in Mexico (2007–2011) . Infection, Genetics and Evolution. 65. p. 12-14.
Borca, M.V., O&#39;Donnell, V., Holinka, L.G., Sanford, B., Azzinaro, P.A., Risatti, G.R., Gladue, D.P.(2017). Development of a fluorescent ASFV strain that retains the ability to cause disease in swine . Scientific Reports. 7.
Holinka, L.G., O&#39;Donnell, V., Risatti, G.R., Azzinaro, P., Arzt, J., Stenfeldt, C., Velazquez-Salinas, L., Carlson, J., Gladue, D.P., Borca, M.V.(2017). Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G . PLoS ONE. 12. (5).
O&#39;Donnell, V., Risatti, G.R., Holinka, L.G., Krug, P.W., Carlson, J., Velazquez-Salinas, L., Azzinaro, P.A., Gladue, D.P., Borca, M.V.(2017). Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate offers increased safety and protection against homologous challenge . Journal of Virology. 91. (1).
Douglas Gladue, Jolene Carlson, Vivian O’Donnell, Marialexia Alfano, Lauro Velazquez Salinas, Lauren Holinka, Peter Krug, Stephen Higgs, Manuel Borca(2016). Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model . Viruses. 8. (12). p. 291. {MDPI} {AG}
(2016). Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model . Viruses.
Manuel V. Borca, Vivian O’Donnell, Lauren G. Holinka, Devendra K. Rai, Brenton Sanford, Marialexia Alfano, Jolene Carlson, Paul A. Azzinaro, Covadonga Alonso, Douglas P. Gladue(2016). The Ep152R ORF of African swine fever virus strain Georgia encodes for an essential gene that interacts with host protein BAG6 . Virus Research. 223. p. 181--189. Elsevier {BV}
O'Donnell V, Holinka LG, Sanford B, Krug PW, Carlson J, Pacheco JM, Reese B, Risatti GR, Gladue DP, Borca MV, Virus research, 2016, vol. 221, pp. 8-14 .
Velazquez-Salinas L, Risatti GR, Holinka LG, O'Donnell V, Carlson J, Alfano M, Rodriguez LL, Carrillo C, Gladue DP, Borca MV, Virology, 2016, vol. 494, pp. 178-189 .
Sanford B, Holinka LG, O'Donnell V, Krug PW, Carlson J, Alfano M, Carrillo C, Wu P, Lowe A, Risatti GR, Gladue DP, Borca MV, Virus research, 2016, vol. 213, pp. 165-171 .
Holinka, L.G., Largo, E., Gladue, D.P., O&#39;Donnell, V., Risatti, G.R., Nieva, J.L., Borca, M.V.(2016). Alteration of a second putative fusion peptide of structural glycoprotein E2 of classical swine fever virus alters virus replication and virulence in swine . Journal of Virology. 90. (22). p. 10299-10308.
Velazquez-Salinas, L., Zarate, S., Eschbaumer, M., Lobo, F.P., Gladue, D.P., Arzt, J., Novella, I.S., Rodriguez, L.L.(2016). Selective factors associated with the evolution of codon usage in natural populations of arboviruses . PLoS ONE. 11. (7).
Vivian O&#39;Donnell, Lauren G. Holinka, Peter W. Krug, Douglas P. Gladue, Jolene Carlson, Brenton Sanford, Marialexia Alfano, Edward Kramer, Zhiqiang Lu, Jonathan Arzt, et al.(2015). African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge . J. Virol. 89. (16). p. 8556--8566. American Society for Microbiology
Vivian O&#39;Donnell, Lauren G. Holinka, Douglas P. Gladue, Brenton Sanford, Peter W. Krug, Xiqiang Lu, Jonathan Arzt, Bo Reese, Consuelo Carrillo, Guillermo R. Risatti, et al.(2015). African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus . J. Virol. 89. (11). p. 6048--6056. American Society for Microbiology
O&#39;Donnell, V., Holinka, L.G., Gladue, D.P., Sanford, B., Krug, P.W., Lu, X., Arzt, J., Reese, B., Carrillo, C., Risatti, G.R., et al.(2015). African swine fever virus Georgia isolate harboring deletions of MGF360 and MGF505 genes is attenuated in swine and confers protection against challenge with virulent parental virus . Journal of Virology. 89. (11). p. 6048-6056.
O&#39;Donnell, V., Holinka, L.G., Krug, P.W., Gladue, D.P., Carlson, J., Sanford, B., Alfano, M., Kramer, E., Lu, Z., Arzt, J., et al.(2015). African swine fever virus Georgia 2007 with a deletion of virulence-associated gene 9GL (B119L), when administered at low doses, leads to virus attenuation in swine and induces an effective protection against homologous challenge . Journal of Virology. 89. (16). p. 8556-8566.
Krug, P.W., Holinka, L.G., O&#39;Donnell, V., Reese, B., Sanford, B., Fernandez-Sainz, I., Gladue, D.P., Arzt, J., Rodriguez, L., Risatti, G.R., et al.(2015). The progressive adaptation of a Georgian isolate of African swine fever virus to Vero cells leads to a gradual attenuation of virulence in swine corresponding to major modifications of the viral genome . Journal of Virology. 89. (4). p. 2324-2332.
Holinka LG, Fernandez-Sainz I, Sanford B, O'Donnell V, Gladue DP, Carlson J, Lu Z, Risatti GR, Borca MV, Virology, 2014, vol. 471-473, pp. 13-18 .
O'Donnell V, Pacheco JM, Larocco M, Gladue DP, Pauszek SJ, Smoliga G, Krug PW, Baxt B, Borca MV, Rodriguez L, Virology, 2014, vol. 468-470, pp. 185-196 .
Gladue DP, O'Donnell V, Fernandez-Sainz IJ, Fletcher P, Baker-Branstetter R, Holinka LG, Sanford B, Carlson J, Lu Z, Borca MV, Virology, 2014, vol. 460-461, pp. 173-179 .
Fernández-Sainz IJ, Largo E, Gladue DP, Fletcher P, O'Donnell V, Holinka LG, Carey LB, Lu X, Nieva JL, Borca MV, Virology, 2014, vol. 456-457, pp. 121-130 .
Gladue DP, O'Donnell V, Baker-Bransetter R, Pacheco JM, Holinka LG, Arzt J, Pauszek S, Fernandez-Sainz I, Fletcher P, Brocchi E, Lu Z, Rodriguez LL, Borca MV, Journal of virology, 2014, vol. 88, no. 5, pp. 2737-2747 .
Douglas P. Gladue, Ryan Baker-Bransetter, Lauren G. Holinka, Ignacio J. Fernandez-Sainz, Vivian O’Donnell, Paige Fletcher, Zhiqiang Lu, Manuel V. Borca, Volker Thiel(2014). Interaction of CSFV E2 Protein with Swine Host Factors as Detected by Yeast Two-Hybrid System . PLoS ONE. 9. (1). p. e85324. Public Library of Science ({PLoS})
Eneko Largo, Douglas P. Gladue, Nerea Huarte, Manuel V. Borca, Jos&#233; L. Nieva(2014). Pore-forming activity of pestivirus p7 in a minimal model system supports genus-specific viroporin function . Antiviral Research. 101. p. 30--36. Elsevier {BV}
Peter W. Krug, Lauren G. Holinka, Vivian O&#39;Donnell, Bo Reese, Brenton Sanford, Ignacio Fernandez-Sainz, Douglas P. Gladue, Jonathan Arzt, Luis Rodriguez, Guillermo R. Risatti, et al.(2014). The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome . J. Virol. 89. (4). p. 2324--2332. American Society for Microbiology
Juan M. Pacheco, Douglas P. Gladue, Lauren G. Holinka, Jonathan Arzt, Elizabeth Bishop, George Smoliga, Steve J. Pauszek, Alexa J. Bracht, Vivian O&#39;Donnell, Ignacio Fernandez-Sainz, et al.(2013). A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle . Virology. 446. (1-2). p. 260--267. Elsevier {BV}
Gladue DP, O'Donnell V, Baker-Branstetter R, Holinka LG, Pacheco JM, Fernández Sainz I, Lu Z, Ambroggio X, Rodriguez L, Borca MV, <i>Journal of virology</i>, 2013, vol. 87, no. 12, pp. 6794-6803 .
Gladue DP, O'Donnell V, Baker-Branstetter R, Holinka LG, Pacheco JM, Fernandez-Sainz I, Lu Z, Brocchi E, Baxt B, Piccone ME, Rodriguez L, Borca MV, <i>Journal of virology</i>, 2012, vol. 86, no. 22, pp. 12080-12090 .
Gladue DP, Holinka LG, Largo E, Fernandez Sainz I, Carrillo C, O'Donnell V, Baker-Branstetter R, Lu Z, Ambroggio X, Risatti GR, Nieva JL, Borca MV, <i>Journal of virology</i>, 2012, vol. 86, no. 12, pp. 6778-6791 .
Fernández-Sainz I, Holinka LG, Gladue D, O'Donnell V, Lu Z, Gavrilov BK, Risatti GR, Borca MV, <i>Journal of virology</i>, 2011, vol. 85, no. 14, pp. 7264-7272 .
Gladue DP, Gavrilov BK, Holinka LG, Fernandez-Sainz IJ, Vepkhvadze NG, Rogers K, O'Donnell V, Risatti GR, Borca MV, <i>Virology</i>, 2011, vol. 411, no. 1, pp. 41-49 .
Gladue DP, Holinka LG, Fernandez-Sainz IJ, Prarat MV, O'Donnell V, Vepkhvadze NG, Lu Z, Risatti GR, Borca MV, <i>Virology</i>, 2011, vol. 412, no. 1, pp. 68-74 .
Gladue DP, Holinka LG, Fernandez-Sainz IJ, Prarat MV, O'Donell V, Vepkhvadze N, Lu Z, Rogers K, Risatti GR, Borca MV, <i>Virology</i>, 2010, vol. 407, no. 1, pp. 129-136 .
Gladue DP, Zhu J, Holinka LG, Fernandez-Sainz I, Carrillo C, Prarat MV, O'Donnell V, Borca MV, <i>Virus research</i>, 2010, vol. 151, no. 1, pp. 10-18 .
Fernandez-Sainz I, Gladue DP, Holinka LG, O'Donnell V, Gudmundsdottir I, Prarat MV, Patch JR, Golde WT, Lu Z, Zhu J, Carrillo C, Risatti GR, Borca MV, <i>Journal of virology</i>, 2010, vol. 84, no. 3, pp. 1536-1549 .
Fernandez-Sainz I, Holinka LG, Gavrilov BK, Prarat MV, Gladue D, Lu Z, Jia W, Risatti GR, Borca MV, <i>Virology</i>, 2009, vol. 386, no. 1, pp. 210-216 .
Holinka LG, Fernandez-Sainz I, O'Donnell V, Prarat MV, Gladue DP, Lu Z, Risatti GR, Borca MV, <i>Virology</i>, 2009, vol. 384, no. 1, pp. 106-113 .
A. Vannini, C. Gladue(2008). Moccasin on One Foot, High Heel on the Other: Life Story Reflections of Coreen Gladue . Qualitative Inquiry. 15. (4). p. 675--720. {SAGE} Publications
Gladue DP, Konopka JB, <i>FEMS yeast research</i>, 2008, vol. 8, no. 1, pp. 71-80 .
Ballon DR, Flanary PL, Gladue DP, Konopka JB, Dohlman HG, Thorner J, <i>Cell</i>, 2006, vol. 126, no. 6, pp. 1079-1093 .