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Hire DeBroski H.

United States

USD 150 /hr

Immunologist that specializes in Infectious disease and allergic reactions

Profile Summary

The over-arching goal of my research program is to uncover new information that increases the basic understanding of the cellular and molecular mechanisms controlling pathogen-specific immunity at the mucosal interface. While my work has encompassed a variety of organisms, there is a major focus on parasitic helminths (worms), which are a major cause of morbidity in both human and animal populations across the globe. The work we have published through collaborative efforts and those spearheaded in my own laboratory has produced 60+ publications to date, with important contributions that have increased the understanding of key immunological cell types including: alternatively activated /M2 macrophages, eosinophil effector functions, Type 2 innate lymphocytes (ILC2), and conventional T helper 2 cells (TH2). A major theme of my program is the process of wound healing, and my work has helped to address important major gaps in the understanding reparative cytokines in the Trefoil factor family (TFF). One of our recent publications identified a family of orphan type 1 transmembrane proteins in the leucine rich repeat and nogo interacting protein family (LINGO) as putative TFF receptors, which shed's important light in this poorly understood field. Additionally, my laboratory has active ongoing projects focused on human helminth infection, dendritic cells as unconventional cellular sources of interleukin 33 (IL-33), Wnt pathway signaling, and transgenesis in parasitic nematodes to study the basic tenets of antigen-specific CD4+ T cell biology. Collectively, this research program uses a combination of murine, veterinary animal, and human model systems to shed new light upon parasite-host interactions, mucosal immunology, and regenerative medicine.

Subject Matter Expertise

• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Molecular Biology
• ☆ Basic Immunology
• ☆ Adjuvant & Antigen Research
• ☆ Immunohistochemistry

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing, Copywriting

Research Market Research, Feasibility Study, Fact Checking, Gap Analysis, Systematic Literature Review

Consulting Digital Strategy Consulting, Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization, Big Data Analytics, Data Insights

Product Development Product Evaluation, Concept Development

Work Experience

Professor

University of Pennsylvania

April 2022 - Present

University of Pennsylvania

April 2016 - Present

Assistant Professor

University of California San Francisco

April 2012 - March 2016

Assistant Professor

Cincinnati Children's Hospital Medical Center

2009 - 2012

Research assistant Professor

University of Cincinnati

2006 - 2009

Education

Thomas Jefferson University

June 1996 - December 2000

B.S. (Microbiology)

Xavier University of Louisiana

September 1990 - June 1994

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Heather L. Rossi, Jorge F. Ortiz-Carpena, Devon Tucker, Andrew E. Vaughan, Nilam S. Mangalmurti, Noam A. Cohen, De’Broski R. Herbert(2022). Trefoil Factor Family: A Troika for Lung Repair and Regeneration . American Journal of Respiratory Cell and Molecular Biology. 66. (3). p. 252--259. American Thoracic Society

Fungai Musaigwa, Meera Goh Nair, Severin Donald Kamdem, Thabo Mpotje, Paballo Mosala, Nada Abdel Aziz, De’Broski R. Herbert, Frank Brombacher, Justin Komguep Nono (2022). Schistosoma mansoni infection induces plasmablast and plasma cell death in the bone marrow and accelerates the decline of host vaccine responses . PLOS Pathogens.

Babatunde Adewale, Subash Babu, Jonathan R. Heintz, Christopher F. Pastore, Heather L. Rossi, Li-Yin Hung, Nurudeen Rahman, Jeff Bethony, David Diemert, James Ayorinde Babatunde, et al.(2021). Parasitic helminth infections in humans modulate Trefoil Factor levels in a manner dependent on the species of parasite and age of the host . PLOS Neglected Tropical Diseases. 15. (10). p. e0009550. Public Library of Science ({PLoS})

Juan M. Inclan-Rico, De’Broski R. Herbert(2021). T Regulatory Cells Influence Decisions between Concomitant Immunity versus Sterile Cure . The Journal of Immunology. 207. (1). p. 3--4. The American Association of Immunologists

Hung LY, Tanaka Y, Herbine K, Pastore C, Singh B, Ferguson A, Vora N, Douglas B, Zullo K, Behrens EM, et al.(2020). Cellular context of IL-33 expression dictates impact on anti-helminth immunity . Science immunology.

Braun JD, Lozada IB, Herbert DE(2020). In Pursuit of Panchromatic Absorption in Metal Coordination Complexes: Experimental Delineation of the HOMO Inversion Model Using Pseudo-Octahedral Complexes of Diarylamido Ligands . Inorganic chemistry.

Hung LY, Pastore CF, Douglas B, Herbert DR(2020). Myeloid-Derived IL-33 Limits the Severity of Dextran Sulfate Sodium-Induced Colitis . The American journal of pathology.

Sell EA, Ortiz-Carpena JF, Herbert DR, Cohen NA(2020). Tuft cells in the pathogenesis of chronic rhinosinusitis with nasal polyps and asthma . Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology.

Jackson SR, Costa MFDM, Pastore CF, Zhao G, Weiner AI, Adams S, Palashikar G, Quansah K, Hankenson K, Herbert DR, et al.(2020). R-spondin 2 mediates neutrophil egress into the alveolar space through increased lung permeability . BMC research notes.

Li-Yin Hung, John L. Johnson, Yingbiao Ji, David A. Christian, Karl R. Herbine, Christopher F. Pastore, De’Broski R. Herbert(2019). Cell-Intrinsic Wnt4 Influences Conventional Dendritic Cell Fate Determination to Suppress Type 2 Immunity . The Journal of Immunology. p. ji1900363. The American Association of Immunologists

De’Broski Herbert, Bonnie Douglas, Kelly Zullo(2019). Group 2 Innate Lymphoid Cells (ILC2): Type 2 Immunity and Helminth Immunity . International Journal of Molecular Sciences. 20. (9). p. 2276. {MDPI} {AG}

De'Broski Herbert, Bonnie Douglas, Kelly Zullo (2019). Group 2 Innate Lymphoid Cells (ILC2): Type 2 Immunity and Helminth Immunity . International Journal of Molecular Sciences.

Patel, N.N., Kohanski, M.A., Maina, I.W., Workman, A.D., Herbert, D.R., Cohen, N.A.(2018). Sentinels at the wall: epithelial-derived cytokines serve as triggers of upper airway type 2 inflammation . International Forum of Allergy and Rhinology.

Kohanski, M.A., Workman, A.D., Patel, N.N., Hung, L.-Y., Shtraks, J.P., Chen, B., Blasetti, M., Doghramji, L., Kennedy, D.W., Adappa, N.D., et al.(2018). Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps . Journal of Allergy and Clinical Immunology. 142. (2). p. 460-469.e7.

Patel, N.N., Kohanski, M.A., Maina, I.W., Triantafillou, V., Workman, A.D., Tong, C.C.L., Kuan, E.C., Bosso, J.V., Adappa, N.D., Palmer, J.N., et al.(2018). Solitary chemosensory cells producing interleukin-25 and group-2 innate lymphoid cells are enriched in chronic rhinosinusitis with nasal polyps . International Forum of Allergy and Rhinology. 8. (8). p. 900-906.

Hung, L.-Y., Oniskey, T.K., Sen, D., Krummel, M.F., Vaughan, A.E., Cohen, N.A., Herbert, D.R.(2018). Trefoil Factor 2 Promotes Type 2 Immunity and Lung Repair through Intrinsic Roles in Hematopoietic and Nonhematopoietic Cells . American Journal of Pathology. 188. (5). p. 1161-1170.

Stoltzfus, J.D., Pilgrim, A.A., Herbert, D.R.(2017). Perusal of parasitic nematode ‘omics in the post-genomic era . Molecular and Biochemical Parasitology. 215. p. 11-22.

Wildaliz Nieves, Li-Yin Hung, Taylor K. Oniskey, Louis Boon, Marc Foretz, Benoit Viollet, De’Broski R. Herbert(2016). Myeloid-Restricted AMPKα1 Promotes Host Immunity and Protects against IL-12/23p40–Dependent Lung Injury during Hookworm Infection . The Journal of Immunology. 196. (11). p. 4632--4640. The American Association of Immunologists

Chan, P.Y., Carrera Silva, E.A., De Kouchkovsky, D., Joannas, L.D., Hao, L., Hu, D., Huntsman, S., Eng, C., Licona-Limón, P., Weinstein, J.S., et al.(2016). The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity . Science. 352. (6281). p. 99-103.

Karo-Atar, D., Bordowitz, A., Wand, O., Pasmanik-Chor, M., Fernandez, I.E., Itan, M., Frenkel, R., Herbert, D.R., Finkelman, F.D., Eickelberg, O., et al.(2016). A protective role for IL-13 receptor α 1 in bleomycin-induced pulmonary injury and repair . Mucosal Immunology. 9. (1). p. 240-253.

Nair, M.G., Herbert, D.R.(2016). Immune polarization by hookworms: Taking cues from T helper type 2, type 2 innate lymphoid cells and alternatively activated macrophages . Immunology. 148. (2). p. 115-124.

Nieves, W., Hung, L.-Y., Oniskey, T.K., Boon, L., Foretz, M., Viollet, B., Herbert, D.R.(2016). Myeloid-restricted AMPKa1 promotes host immunity and protects against IL-12/23p40-dependent lung injury during hookworm infection . Journal of Immunology. 196. (11). p. 4632-4640.

Fontana, M.F., Baccarella, A., Pancholi, N., Pufall, M.A., Herbert, D.R., Kim, C.C.(2015). JUNB is a key transcriptional modulator of macrophage activation . Journal of Immunology. 194. (1). p. 177-186.

Fontana, M.F., Baccarella, A., Kellar, D., Oniskey, T.K., Terinate, P., Rosenberg, S.D., Huang, E.J., Herbert, D.R., Kim, C.C.(2015). Myeloid expression of the AP-1 transcription factor JUNB modulates outcomes of type 1 and type 2 parasitic infections . Parasite Immunology. 37. (9). p. 470-478.

Gagliani, N., Magnani, C.F., Huber, S., Gianolini, M.E., Pala, M., Licona-Limon, P., Guo, B., Herbert, D.R., Bulfone, A., Trentini, F., et al.(2014). Erratum: Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells (Nature Medicine (2013) 19 (739-746)) . Nature Medicine. 20. (10).

Mcberry, C., Dias, A., Shryock, N., Lampe, K., Gutierrez, F.R.S., Boon, L., Herbert, D.R., Aliberti, J.(2014). PD-1 modulates steady-state and infection-induced IL-10 production in vivo . European Journal of Immunology. 44. (2). p. 469-479.

Gagliani, N., Magnani, C.F., Huber, S., Gianolini, M.E., Pala, M., Licona-Limon, P., Guo, B., Herbert, D.R., Bulfone, A., Trentini, F., et al.(2013). Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells . Nature Medicine. 19. (6). p. 739-746.

Hung, L.-Y., Lewkowich, I.P., Dawson, L.A., Downey, J., Yang, Y., Smith, D.E., Herbert, D.R.(2013). IL-33 drives biphasic IL-13 production for noncanonical Type 2 immunity against hookworms . Proceedings of the National Academy of Sciences of the United States of America. 110. (1). p. 282-287.

You, D., Marr, N., Saravia, J., Shrestha, B., Lee, G.I., Turvey, S.E., Brombacher, F., Herbert, D.R., Cormier, S.A.(2013). IL-4R? on CD4⁺T cells plays a pathogenic role in respiratory syncytial virus reinfection in mice infected initially as neonates . Journal of Leukocyte Biology. 93. (6). p. 933-942.

Licona-Limón, P., Henao-Mejia, J., Temann, A.U., Gagliani, N., Licona-Limón, I., Ishigame, H., Hao, L., Herbert, D.R., Flavell, R.A.(2013). Th9 cells drive host immunity against gastrointestinal worm infection . Immunity. 39. (4). p. 744-757.

Fu, C.-L., Odegaard, J.I., Herbert, D.R., Hsieh, M.H.(2012). A novel mouse model of schistosoma haematobium egg-induced immunopathology . PLoS Pathogens. 8. (3).

Rani, R., Jordan, M.B., Divanovic, S., Herbert, D.R.(2012). IFN-γ-driven IDO production from macrophages protects IL-4Rα-deficient mice against lethality during schistosoma mansoni infection . American Journal of Pathology. 180. (5). p. 2001-2008.

Heitmann, L., Rani, R., Dawson, L., Perkins, C., Yang, Y., Downey, J., Hlscher, C., Herbert, D.R.(2012). TGF-βresponsive myeloid cells suppress type 2 immunity and emphysematous pathology after hookworm infection . American Journal of Pathology. 181. (3). p. 897-906.

McBerry, C., Egan, C.E., Rani, R., Yang, Y., Wu, D., Boespflug, N., Boon, L., Butcher, B., Mirpuri, J., Hogan, S.P., et al.(2012). Trefoil factor 2 negatively regulates type 1 immunity against Toxoplasma gondii . Journal of Immunology. 189. (6). p. 3078-3084.

Wills-Karp, M., Rani, R., Dienger, K., Lewkowich, I., Fox, J.G., Perkins, C., Lewis, L., Finkelman, F.D., Smith, D.E., Bryce, P.J., et al.(2012). Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection . Journal of Experimental Medicine. 209. (3). p. 607-622.

Rani, R., Smulian, A.G., Greaves, D.R., Hogan, S.P., Herbert, D.R.(2011). TGF-β limits IL-33 production and promotes the resolution of colitis through regulation of macrophage function . European Journal of Immunology. 41. (7). p. 2000-2009.

Butcher, B.A., Fox, B.A., Rommereim, L.M., Kim, S.G., Maurer, K.J., Yarovinsky, F., Herbert, D.R., Bzik, D.J., Denkers, E.Y.(2011). Toxoplasma gondii rhoptry kinase rop16 activates stat3 and stat6 resulting in cytokine inhibition and arginase-1-dependent growth control . PLoS Pathogens. 7. (9).

Herbert, D.R., Orekov, T., Roloson, A., Ilies, M., Perkins, C., O'Brien, W., Cederbaum, S., Christianson, D.W., Zimmermann, N., Rothenberg, M.E., et al.(2010). Arginase I suppresses IL-12/IL-23p40-driven intestinal inflammation during acute schistosomiasis . Journal of Immunology. 184. (11). p. 6438-6446.

Nieuwenhuizen, N., Herbert, D.R., Brombacher, F., Lopata, A.L.(2009). Differential requirements for interleukin (IL)-4 and IL-13 in protein contact dermatitis induced by Anisakis . Allergy: European Journal of Allergy and Clinical Immunology. 64. (9). p. 1309-1318.

Morris, S.C., Heidorn, S.M., Herbert, D.R., Perkins, C., Hildeman, D.A., Khodoun, M.V., Finkelman, F.D.(2009). Endogenously produced IL-4 nonredundantly stimulates CD8⁺T cell proliferation . Journal of Immunology. 182. (3). p. 1429-1438.

O'Connell, A.E., Kerepesi, L.A., Vandergrift, G.L., Herbert, D.R., Van Winkle, T.J., Hooper, D.C., Pearce, E.J., Abraham, D.(2009). IL-4^-/-mice with lethal Mesocestoides corti infections - Reduced Th2 cytokines and alternatively activated macrophages . Parasite Immunology. 31. (12). p. 741-749.

Herbert, D.R., Yang, J.-Q., Hogan, S.P., Groschwitz, K., Khodoun, M., Munitz, A., Orekov, T., Perkins, C., Wang, Q., Brombacher, F., et al.(2009). Intestinal epithelial cell secretion of RELM-β protects against gastrointestinal worm infection . Journal of Experimental Medicine. 206. (13). p. 2947-2957.

Khodoun, M., Strait, R., Orekov, T., Hogan, S., Karasuyama, H., Herbert, D.R., Köhl, J., Finkelman, F.D.(2009). Peanuts can contribute to anaphylactic shock by activating complement . Journal of Allergy and Clinical Immunology. 123. (2). p. 342-351.

Herbert, D.R., Orekov, T., Perkins, C., Finkelman, F.D.(2008). IL-10 and TGF-β redundantly protect against severe liver injury and mortality during acute schistosomiasis . Journal of Immunology. 181. (10). p. 7214-7220.

Herbert, D.R., Orekov, T., Perkins, C., Rothenberg, M.E., Finkelman, F.D.(2008). IL-4Rα expression by bone marrow-derived cells is necessary and sufficient for host protection against acute schistosomiasis . Journal of Immunology. 180. (7). p. 4948-4955.

Nieuwenhuizen, N., Herbert, D.R., Lopata, A.L., Brombacher, F.(2007). CD4+ T cell-specific deletion of IL-4 receptor α prevents ovalbumin-induced anaphylaxis by an IFN-γ-dependent mechanism . Journal of Immunology. 179. (5). p. 2758-2765.

Nieuwenhuizen, N., Lopata, A.L., Jeebhay, M.F., Herbert, D.R., Robins, T.G., Brombacher, F.(2006). Exposure to the fish parasite Anisakis causes allergic airway hyperreactivity and dermatitis . Journal of Allergy and Clinical Immunology. 117. (5). p. 1098-1105.

Nieuwenhuizen, N., Lopata, A.L., Jeebhay, M.F., Herbert, D.R., Robins, T.G., Brombacher, F.(2006). Reply . Journal of Allergy and Clinical Immunology. 118. (4). p. 968-969.

Leeto, M., Herbert, D.R., Marillier, R., Schwegmann, A., Fick, L., Brombacher, F.(2006). T<inf>H</inf>1-dominant granulomatous pathology does not inhibit fibrosis or cause lethality during murine schistosomiasis . American Journal of Pathology. 169. (5). p. 1701-1712.

Herbert, D.R., H&#246;lscher, C., Mohrs, M., Arendse, B., Schwegmann, A., Radwanska, M., Leeto, M., Kirsch, R., Hall, P., Mossmann, H., et al.(2004). Alternative macrophage activation is essential for survival during schistosomiasis and downmodulates T helper 1 responses and immunopathology . Immunity. 20. (5). p. 623-635.

Herbert, D.R., H&#246;lscher, C., Mohrs, M., Arendse, B., Schwegmann, A., Radwanska, M., Leeto, M., Kirsch, R., Hall, P., Mossmann, H., et al.(2004). Erratum: Alternative macrophage activation is essential for survival during schistosomiasis and downmodulates T helper 1 responses and immunopathology (Immunity 20 (623-635)) . Immunity. 21. (3).

Kerepesi, L.A., Nolan, T.J., Schad, G.A., Lustigman, S., Herbert, D.R., Keiser, P.B., Nutman, T.B., Krolewiecki, A.J., Abraham, D.(2004). Human immunoglobulin G mediates protective immunity and identifies protective antigens against larval Strongyloides stercoralis in mice . Journal of Infectious Diseases. 189. (7). p. 1282-1290.

Herbert, D.R., Nolan, T.J., Schad, G.A., Lustigman, S., Abraham, D.(2002). Immunoaffinity-isolated antigens induce protective immunity against larval Strongyloides stercoralis in mice . Experimental Parasitology. 100. (2). p. 112-120.

Herbert, D.R., Nolan, T.J., Schad, G.A., Abraham, D.(2002). The role of B cells in immunity against larval Strongyloides stercoralis in mice . Parasite Immunology. 24. (2). p. 95-101.

Herbert, D.R., Lee, J.J., Lee, N.A., Nolan, T.J., Schad, G.A., Abraham, D.(2000). Role of IL-5 in innate and adaptive immunity to larval Strongyloides stercoralis in mice . Journal of Immunology. 165. (8). p. 4544-4551.

Resnicoff, M., Valentinis, B., Herbert, D., Abraham, D., Friesen, P.D., Alnemri, E.S., Baserga, R.(1998). The baculovirus anti-apoptotic p35 protein promotes transformation of mouse embryo fibroblasts . Journal of Biological Chemistry. 273. (17). p. 10376-10380.

Resnicoff, M., Huang, Z., Herbert, D., Abraham, D., Baserga, R.(1997). A novel class of peptides that induce apoptosis and abrogate tumorigenesis in vivo . Biochemical and Biophysical Research Communications. 240. (1). p. 208-212.

PREPRINT

Ortiz-Carpena JF, Maina IW, Lin C, Patel NN, Triantafillou V, Luu NN, Kohanski MA, Kuan EC, Tong CCL, Bosso JV, et al.(2020). Trefoil factor 3 positively associates with IL-25 in patients with Chronic Rhinosinusitis .

Jackson SR, Costa MFDM, Pastore CF, Zhao G, Weiner AI, Adams S, Palashikar G, Quansah K, Hankenson K, Herbert D, et al.(2020). R-spondin 2 mediates neutrophil egress into the alveolar space through increased lung permeability .

OTHER

Li-Yin Hung, Yingbiao Ji, David A. Christian, Karl R. Herbine, Christopher F. Pastore, De’Broski R. Herbert(2018). Cell-intrinsic Wnt4 controls early cDC1 commitment and suppresses development of pathogen-specific Type 2 immunity . Cold Spring Harbor Laboratory