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USD 100 /hr

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☆☆☆☆☆

Hire Dr. Deanne S.

United States

USD 100 /hr

Experience in computational protein, peptide and RNA design and biophysical characterization

Profile Summary

I have experience generating computational algorithms for protein, peptide and RNA engineering, experience in protein expression, purification and characterization, peptide synthesis and characterization and RNA characterization

Subject Matter Expertise

• ☆ Peptide Synthesis
• ☆ Biochemistry
• ☆ Biochemical Assays
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ RNA Synthesis
• ☆ Biophysics
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Bioinformatics
• ☆ Gene & Protein Expression Analysis
• ☆ Biomolecular Engineering
• ☆ Protein/Enzyme Engineering

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing

Research Technology Scouting, Scientific and Technical Research

Consulting Scientific and Technical Consulting

Data & AI Predictive Modeling, Algorithm Design-Non ML, Data Visualization, Big Data Analytics

Product Development Product Evaluation, Product Validation

Work Experience

Senior Principal Scientist

Pfizer

September 2022 - October 2024

Director of Protein Engineering

Molecular Assemblies

May 2020 - September 2022

AI Research Scientist

National Renewable Energy Laboratory

January 2011 - August 2019

Education

Postdoctoral Research in Chemistry and Biochemistry

University of Colorado, Boulder

August 2008 - January 2011

Biophysics and Biochemistry

University of North Carolina, Chapel Hill

September 2002 - August 2008

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

DeChellis, A., Nemmaru, B., Sammond, D., Douglass, J., Patil, N., Reste, O., Chundawat, S.P.S.(2024). Supercharging Carbohydrate Binding Module Alone Enhances Endocellulase Thermostability, Binding, and Activity on Cellulosic Biomass . ACS Sustainable Chemistry and Engineering. 12. (9). p. 3500-3516.

Chung, D., Sarai, N.S., Knott, B.C., Hengge, N., Russell, J.F., Yarbrough, J.M., Brunecky, R., Young, J., Supekar, N., Vander Wall, T., et al.(2019). Glycosylation Is Vital for Industrial Performance of Hyperactive Cellulases . ACS Sustainable Chemistry and Engineering. 7. (5). p. 4792-4800.

Sammond, D.W., Kastelowitz, N., Donohoe, B.S., Alahuhta, M., Lunin, V.V., Chung, D., Sarai, N.S., Yin, H., Mittal, A., Himmel, M.E., et al.(2018). An iterative computational design approach to increase the thermal endurance of a mesophilic enzyme . Biotechnology for Biofuels. 11. (1).

Haarmeyer, C.N., Smith, M.D., Chundawat, S.P.S., Sammond, D., Whitehead, T.A.(2017). Insights into cellulase-lignin non-specific binding revealed by computational redesign of the surface of green fluorescent protein . Biotechnology and Bioengineering. 114. (4). p. 740-750.

Brunecky, R., Alahuhta, M., Sammond, D.W., Xu, Q., Chen, M., Wilson, D.B., Brady, J.W., Himmel, M.E., Bomble, Y.J., Lunin, V.V.(2017). Natural diversity of glycoside hydrolase family 48 exoglucanases: Insights from structure Mike Himmel . Biotechnology for Biofuels. 10. (1).

Sammond, D.W., Kastelowitz, N., Himmel, M.E., Yin, H., Crowley, M.F., Bomble, Y.J.(2016). Comparing residue clusters from thermophilic and mesophilic enzymes reveals adaptive mechanisms . Plos One. 11. (1).

Qin, Y., Sammond, D.W., Braselmann, E., Carpenter, M.C., Palmer, A.E.(2016). Development of an Optical Zn2+ Probe Based on a Single Fluorescent Protein . ACS Chemical Biology. 11. (10). p. 2744-2751.

Yarbrough, J.M., Mittal, A., Mansfield, E., Taylor, L.E., Hobdey, S.E., Sammond, D.W., Bomble, Y.J., Crowley, M.F., Decker, S.R., Himmel, M.E., et al.(2015). New perspective on glycoside hydrolase binding to lignin from pretreated corn stover . Biotechnology for Biofuels. 8. (1).

Alahuhta, M., Taylor, L.E., Brunecky, R., Sammond, D.W., Michener, W., Adams, M.W.W., Himmel, M.E., Bomble, Y.J., Lunin, V.(2015). The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase . Acta Crystallographica Section D Biological Crystallography. 71. p. 1946-1954.

Sammond, D.W., Yarbrough, J.M., Mansfield, E., Bomble, Y.J., Hobdey, S.E., Decker, S.R., Taylor, L.E., Resch, M.G., Bozell, J.J., Himmel, M.E., et al.(2014). Predicting enzyme adsorption to lignin films by calculating enzyme surface hydrophobicity . Journal of Biological Chemistry. 289. (30). p. 20960-20969.

Lee, J., Sammond, D.W., Fiorini, Z., Saludes, J.P., Resch, M.G., Hao, B., Wang, W., Yin, H., Liu, X.(2013). Computationally Designed Peptide Inhibitors of the Ubiquitin E3 Ligase SCFFbx4 . Chembiochem. 14. (4). p. 445-451.

Sammond, D.W., Payne, C.M., Brunecky, R., Himmel, M.E., Crowley, M.F., Beckham, G.T.(2012). Cellulase linkers are optimized based on domain type and function: insights from sequence analysis, biophysical measurements, and molecular simulation . Plos One. 7. (11).

Sammond, D.W., Bosch, D.E., Butterfoss, G.L., Purbeck, C., MacHius, M., Siderovski, D.P., Kuhlman, B.(2011). Computational design of the sequence and structure of a protein-binding peptide . Journal of the American Chemical Society. 133. (12). p. 4190-4192.

Bosch, D.E., Kimple, A.J., Sammond, D.W., Muller, R.E., Miley, M.J., Machius, M., Kuhlman, B., Willard, F.S., Siderovski, D.P.(2011). Structural determinants of affinity enhancement between GoLoco motifs and G-protein α subunit mutants . Journal of Biological Chemistry. 286. (5). p. 3351-3358.

Sammond, D.W., Joce, C., Takeshita, R., McQuate, S.E., Ghosh, N., Martin, J.M., Yin, H.(2011). Transmembrane peptides used to investigate the homo-oligomeric interface and binding hotspot of latent membrane protein 1 . Biopolymers. 95. (11). p. 772-784.

Sammond, D.W., Eletr, Z.M., Purbeck, C., Kuhlman, B.(2010). Computational design of second-site suppressor mutations at protein-protein interfaces . Proteins Structure Function and Bioinformatics. 78. (4). p. 1055-1065.

Slivka, P.F., Shridhar, M., Lee, G.-I., Sammond, D.W., Hutchinson, M.R., Martinko, A.J., Buchanan, M.M., Sholar, P.W., Kearney, J.J., Harrison, J.A., et al.(2009). A peptide antagonist of the TLR4-MD2 interaction . Chembiochem. 10. (4). p. 645-649.

Sammond, D.W., Eletr, Z.M., Purbeck, C., Kimple, R.J., Siderovski, D.P., Kuhlman, B.(2007). Structure-based Protocol for Identifying Mutations that Enhance Protein-Protein Binding Affinities . Journal of Molecular Biology. 371. (5). p. 1392-1404.

Urbanc, B., Cruz, L., Ding, F., Sammond, D., Khare, S., Buldyrev, S.V., Stanley, H.E., Dokholyan, N.V.(2004). Molecular dynamics simulation of amyloid β dimer formation . Biophysical Journal. 87. (4). p. 2310-2321.

Jurevics, H., Hostettler, J., Sammond, D.W., Nave, K.-A., Toews, A.D., Morell, P.(2003). Normal metabolism but different physical properties of myelin from mice deficient in proteolipid protein . Journal of Neuroscience Research. 71. (6). p. 826-834.

Jurevics, H., Largent, C., Hostettler, J., Sammond, D.W., Matsushima, G.K., Kleindienst, A., Toews, A.D., Morell, P.(2002). Alterations in metabolism and gene expression in brain regions during cuprizone-induced demyelination and remyelination . Journal of Neurochemistry. 82. (1). p. 126-136.

Jurevics, H., Hostettler, J., Muse, E.D., Sammond, D.W., Matsushima, G.K., Toews, A.D., Morell, P.(2001). Cerebroside synthesis as a measure of the rate of remyelination following cuprizone-induced demyelination in brain . Journal of Neurochemistry. 77. (4). p. 1067-1076.

OTHER

DeChellis, A., Nemmaru, B., Sammond, D., Douglass, J., Patil, N., Reste, O., Chundawat, S.P.S.(2023). Supercharging carbohydrate-binding module alone enhances endocellulase thermostability, binding, and activity on cellulosic biomass . Biorxiv.