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Hire Dr. Daya Ram P.

Nepal

USD 30 /hr

Biochemistry Professor & Research Expert | 25+ Years in Medical Sciences | Metabolic Disorders & Biomarker Specialist

Profile Summary

Experienced biochemistry professor and researcher with over 25 years in medical education and biomedical research. Currently serving as Head of Department of Biochemistry at Manipal College of Medical Sciences, Nepal, affiliated with Kathmandu University. Core Expertise: Medical/Clinical Biochemistry: Extensive experience in cardiometabolic disorders, diabetes biomarkers, and cardiovascular biochemistry Molecular Biology & Cell Biology: Advanced laboratory techniques including immunology, phytochemistry, and molecular parasitology Research & Publications: Author of 25+ peer-reviewed publications in Q1/Q2 journals with focus on metabolic syndrome, diabetes, and novel biomarkers Educational Leadership: Curriculum development for medical programs, student supervision (MD, MSc, PhD levels), and innovative teaching methodologies Recent Research Achievements: Pioneering work on Waist-Triglyceride Index and Triglyceride-Glucose Index for metabolic syndrome detection Novel research on circulating plasma microRNAs as biomarkers for prediabetes and type 2 diabetes Establishment of reference intervals for liver and renal function tests in Nepalese population Technical Skills: Advanced laboratory management in tertiary hospital settings Statistical analysis and data interpretation Grant writing and project management (NAST, UGC, ICIMOD grants) Research methodology and study design Medical writing and scientific communication International Training: Institut Pasteur, France (Vaccinology) WHO-IARC, Lyon (Molecular epidemiology) University of Delaware (Problem-Based Learning) National Cancer Institute, USA (Molecular Prevention of Cancer) Available Services: Research design and methodology consultation Statistical analysis and data interpretation Medical writing and manuscript preparation Literature reviews and systematic reviews Grant proposal writing Curriculum development for medical programs Scientific editing and peer review Biomarker discovery and validation studies Languages: Fluent in English, Hindi, and Nepali

Subject Matter Expertise

• ☆ Phytochemistry
• ☆ Biochemistry
• ☆ Cell Biology
• ☆ Cytochemistry
• ☆ Neuroscience
• ☆ Neurochemistry

Services

• Writing
• Research
• Consulting
• Data & AI

Writing Medical Writing

Research Scientific and Technical Research, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization, Data Processing

Work Experience

Professor

Manipal College of Medical Sciences

July 2017 - Present

Associate Director Academics

Manipal College of Medical Sciences

August 2020 - January 2023

Associate Professor

Universal College of Medical Sciences

January 2007 - February 2010

Assistant Professor

Universal College of Medical Sciences

May 2000 - January 2007

Tutor

Universal College of Medical Sciences

January 1998 - April 2000

Research Assistant

Research Centre for Applied Sciences and Technology (RECAST), Tribhuvan University

March 1997 - January 1998

Education

Diploma in Vaccinology (School of Infectiology)

Institut Pasteur

March 2009 - April 2009

PhD In Biochemistry

Banaras Hindu University

September 2002 - April 2006

MSc

Tribhuvan University Kathmandu - Nepal

August 1992 - October 1994

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Daya Ram Pokharel, Abhishek Maskey, Ramchandra Kafle, Goma Kathayat, Binod Manandhar, Krishna Das Manandhar (2025). Diagnostic potential of waist–triglyceride index, triglyceride–glucose index and related indices for the detection of metabolic syndrome in Nepali adults: A cross-sectional study . Human Nutrition & Metabolism.

Daya Ram Pokharel, Abhishek Maskey, Goma Kathayat, Binod Manandhar, Ramchandra Kafle, Krishna Das Manandhar (2025). Evaluation of novel and traditional anthropometric indices for predicting metabolic syndrome and its components: a cross-sectional study of the Nepali adult population . Scientific Reports.

Daya Ram Pokharel, Abhishek Maskey, Ramchandra Kafle, Ashim Batajoo, Prajwal Dahal, Roji Raut, Shailesh Adhikari, Binod Manandhar, Krishna Das Manandhar (2024). Evaluation of circulating plasma miR-9, miR-29a, miR-192, and miR-375 as potential biomarkers for predicting prediabetes and type 2 diabetes in Nepali adult population . Non-coding RNA Research.

Daya Ram Pokharel, Asmita Sharma, Julia Robinson, Ganesh Dhakal (2024). Establishment of dry-chemistry-based reference intervals of routine liver function tests for the adult population of Gandaki Province, Nepal . PLOS Global Public Health.

Daya Ram Pokharel, Asmita Sharma, Ganesh Dhakal (2024). Establishment of dry-chemistry-based reference intervals of routine liver function tests for the adult population of Gandaki Province, Nepal . PLOS Global Public Health.

Sharma, A., Pokharel, D.R., Dhakal, G.(2024). Establishment of dry-chemistry-based reference intervals of routine liver function tests for the adult population of Gandaki Province, Nepal . PLOS Global Public Health. 4. (5 May).

Kafle, R. C., Sharma, D., & Pokharel, D. R. (2022). Validation of TIMI risk score for STEMI patients visiting a tertiary care hospital of western Nepal. Journal of Gandaki Medical College-Nepal, 15(1), 69–73. https://doi.org/10.3126/jgmcn.v15i1.43993 .

Pokharel, DR (2022). Association between Serum Liver Enzymes and Cardiovascular Diseases: A Case-control Study among Adults with Cardiovascular Disease. Association between Serum Liver Enzymes and Cardiovascular Diseases: A Case-control Study among Adults with Cardiovascular Disease.

Yadav, N.K., Pokharel, D.R., Kathayat, G., Sigdel, M., Hussain, I.(2022). Evaluation of the Diagnostic Potential of Liver Aminotransferases and Alkaline Phosphatase in Patients with Cardiovascular Diseases . Kathmandu University Medical Journal. 20. (77). p. 7-11.

Pokharel, Daya Ram, Yadav, Naval Kishor, Mahaseth, Dipak, Kathayat, Goma, Sigdel, Manoj, Hussain, Imran (2022). Association between Serum Liver Enzymes and Cardiovascular Diseases: A Case-control Study among Adults with Cardiovascular Disease . Journal of Clinical and Diagnostic Research.

Goma Kathayat and Daya Ram Pokharel and Naval Kishor Yadav and Manoj Sigdel(2021). Establishment of dry chemistry based reference intervals of renal function test parameters for the adult population of Kaski District, Nepal . BMC Nephrology. 22. (1). Springer Science and Business Media {LLC}

Kathayat, G., Pokharel, D.R., Yadav, N.K., Sigdel, M.(2021). Establishment of dry chemistry based reference intervals of renal function test parameters for the adult population of Kaski District, Nepal . BMC Nephrology. 22. (1).

Kathayat, G., Pokharel, D.R., Yadav, N.K., Manoj, S.(2021). Association of Anthropometric Parameters with Lipid Profile among Adult Population of Kaski District, Nepal . Kathmandu University Medical Journal. 19. (76). p. 429-435.

Pokharel, DR (2021). Association of anthropometric parameters with lipid profile among adult population of Kaski district, Nepal. Association of anthropometric parameters with lipid profile among adult population of Kaski district, Nepal.

Pokharel, Daya Ram, Kathayat, Goma, Yadav, Naval Kishor, Sigdel, Manoj (2021). Establishment of dry chemistry based reference intervals of renal function test parameters for the adult population of Kaski District, Nepal . BMC Nephrology.

Sanjay Kumar Jha and Naval Kishor Yadav and Daya Ram Pokharel(2020). Effects of Chronic Kidney Disease on Liver Enzymes Activity During Pre and Post Hemodialysis . Journal of Nobel Medical College. 9. (1). p. 51--55. Nepal Journals Online ({JOL})

Daya Ram Pokharel and Dipendra Khadka and Manoj Sigdel and Naval Kishor Yadav and Shreedhar Acharya and Ramchandra Kafle and Ravindra Mohan Sapkota and Tara Sigdel(2017). Prevalence and pattern of dyslipidemia in Nepalese individuals with type 2 diabetes . BMC Research Notes. 10. (1). Springer Science and Business Media {LLC}

Daya Ram Pokharel and Dipendra Khadka and Manoj Sigdel and Naval Kishor Yadav and Ramchandra Kafle and Ravindra Mohan Sapkota and Sanjay Kumar Jha(2017). Association of serum magnesium level with poor glycemic control and renal functions in Nepalese patients with type 2 diabetes mellitus . Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 11. p. S417--S423. Elsevier {BV}

Pokharel, Daya Ram, Khadka, Dipendra, Sigdel, Manoj, Yadav, Naval Kishor, Kafle, Ramchandra, Sapkota, Ravindra Mohan, Jha, Sanjay Kumar (2017). Association of serum magnesium level with poor glycemic control and renal functions in Nepalese patients with type 2 diabetes mellitus . Diabetes & Metabolic Syndrome: Clinical Research & Reviews.

Pokharel, D.R., Khadka, D., Sigdel, M., Yadav, N.K., Kafle, R., Sapkota, R.M., Jha, S.K.(2017). Association of serum magnesium level with poor glycemic control and renal functions in Nepalese patients with type 2 diabetes mellitus . Diabetes and Metabolic Syndrome: Clinical Research and Reviews. 11. p. S417-S423.

Pokharel, D.R., Khadka, D., Sigdel, M., Yadav, N.K., Acharya, S., Kafle, R., Sapkota, R.M., Sigdel, T.(2017). Prevalence and pattern of dyslipidemia in Nepalese individuals with type 2 diabetes . BMC Research Notes. 10. (1). p. 1-11.

DayaRam Pokharel and Manoj Sigdel and NavalKishor Yadav and LokendraBahadur Sapkota and RavindraMohan Sapkota and Dipendra Khadka and Ramchandra Kafle and Sarthak Nepal and Niraj Choudhary(2015). Estimation of 10-year risk of coronary heart disease in nepalese patients with type 2 diabetes: Framingham versus United Kingdom prospective diabetes study . North American Journal of Medical Sciences. 7. (8). p. 347. Medknow

Pokharel, Daya Ram, Khadka, Dipendra, Sigdel, Manoj, Yadav, Naval Kishor, Sapkota, Lokendra Bahadur, Kafle, Ramchandra, Nepal, Sarthak, Sapkota, Ravindra Mohan, Choudhary, Niraj (2015). Estimation of 10-year risk of coronary heart disease in nepalese patients with type 2 diabetes: Framingham versus United Kingdom prospective diabetes study . North American Journal of Medical Sciences.

Pokharel, D.R., Khadka, D., Sigdel, M., Yadav, N.K., Sapkota, L.B., Kafle, R., Nepal, S., Sapkota, R.M., Choudhary, N.(2015). Estimation of 10-year risk of coronary heart disease in Nepalese patients with type 2 diabetes: Framingham versus United Kingdom prospective diabetes study . North American Journal of Medical Sciences. 7. (8). p. 347-355.

Daya Ram Pokharel and Dipendra Khadka and Manoj Sigdel and Naval Kishor Yadav and Shreedhar Acharya and Ram Chandra Kafle and Pramod Shankar Shukla(2014). Prevalence of metabolic syndrome in Nepalese type 2 diabetic patients according to WHO, NCEP ATP III, IDF and Harmonized criteria . Journal of Diabetes & Metabolic Disorders. 13. (1). Springer Science and Business Media {LLC}

Pokharel, Daya Ram, Khadka, Dipendra, Sigdel, Manoj, Yadav, Naval Kishor, Acharya, Shreedhar, Kafle, Ram Chandra, Shukla, Pramod Shankar (2014). Prevalence of metabolic syndrome in Nepalese type 2 diabetic patients according to WHO, NCEP ATP III, IDF and Harmonized criteria . Journal of Diabetes & Metabolic Disorders.

Pokharel, D.R., Khadka, D., Sigdel, M., Yadav, N.K., Acharya, S., Kafle, R.C., Shukla, P.S.(2014). Prevalence of metabolic syndrome in Nepalese type 2 diabetic patients according to WHO, NCEP ATP III, IDF and Harmonized criteria . Journal of Diabetes and Metabolic Disorders. 13. (1).

(2013). Serum total adenosine deaminase activity in Nepalese patients with rheumatoid arthritis . Asian Journal of Medical Sciences.

T. Nagamma and Rinchen Doma Bhutia and Daya Ram Pokharel and Saraswati Yadav and J. Baxi(2012). Influence of Alcohol Consumption on Oxidative Stress and Antioxidant Status in Cancer Patients - Case-control Study from Western Nepal . Asian Pacific Journal of Cancer Prevention. 13. (7). p. 3513--3517. Asian Pacific Organization for Cancer Prevention

DR Pokharel and N Gautam and J Archana and T Nagamma and R Kumar and R M Sapkota(2012). Frequency of Type 2 Diabetes mellitus and Impaired Glycemia in a Teaching Hospital of South-Western Nepal . Asian Journal of Medical Sciences. 2. (3). p. 202--206. Nepal Journals Online ({JOL})

Pokharel, Daya Ram, Nagamma, T., Bhutia, Rinchen Doma, Yadav, Saraswati, Baxi, J. (2012). Influence of Alcohol Consumption on Oxidative Stress and Antioxidant Status in Cancer Patients - Case-control Study from Western Nepal . Asian Pacific Journal of Cancer Prevention.

Nagamma, T., Bhutia, R.D., Pokharel, D.R., Yadav, S., Baxi, J.(2012). Influence of alcohol consumption on oxidative stress and antioxidant status in cancer patients - case-control study from western Nepal . Asian Pacific Journal of Cancer Prevention. 13. (7). p. 3513-3517.

(2011). Effects of cigarette smoking on lipid peroxidation and antioxidant status in cancer patients from Western Nepal. Asian Pacific journal of cancer prevention : APJCP.

Daya Ram Pokharel and Elesela Srikanth and Sushma Rathaur(2009). Screening of different classes of proteases in microfilarial and adult stages of Setaria cervi . Parasitology Research. 104. (6). p. 1399--1405. Springer Science and Business Media {LLC}

Pokharel, D.R., Srikanth, E., Rathaur, S.(2009). Screening of different classes of proteases in microfilarial and adult stages of Setaria cervi . Parasitology Research. 104. (6). p. 1399-1405.

Pokharel, Daya Ram, Srikanth, Elesela, Rathaur, Sushma (2009). Screening of different classes of proteases in microfilarial and adult stages of <i>Setaria cervi</i> . Parasitology Research.

Daya Ram Pokharel and Sushma Rathaur(2008). Purification and characterization of a leucine aminopeptidase from the bovine filarial parasite Setaria cervi . Acta Tropica. 106. (1). p. 1--8. Elsevier {BV}

Pokharel, Daya Ram, Rathaur, Sushma (2008). Purification and characterization of a leucine aminopeptidase from the bovine filarial parasite <i>Setaria cervi</i> . Acta Tropica.

Pokharel, D.R., Rathaur, S.(2008). Purification and characterization of a leucine aminopeptidase from the bovine filarial parasite Setaria cervi . Acta Tropica. 106. (1). p. 1-8.

Daya R Pokharel and Reeta Rai and Pankaj Kumar and C M Chaturvedi and Sushma Rathaur(2006). Tissue localization of collagenase and leucine aminopeptidase in the bovine filarial parasite Setaria cervi . Filaria Journal. 5. (1). Springer Science and Business Media {LLC}

Daya Ram Pokharel and Reeta Rai and Krithika Nandakumar Kodumudi and Maryada Venkata Rami Reddy and Sushma Rathaur(2006). Pokharel DR, Rai R, Kumar KN, Reddy MVR, Rathaur S. (2006). Vaccination with Setaria cervi 175kDa collagenase induces high level of protection against Brugia malayi infection in jirds . Vaccine. 24. (37-39). p. 6208--6215. Elsevier {BV}

Pokharel, D.R., Rai, R., Nandakumar Kodumudi, K., Reddy, M.V.R., Rathaur, S.(2006). Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds . Vaccine. 24. (37-39). p. 6208-6215.

Pokharel, Daya Ram, Rai, Reeta, Kodumudi, Krithika Nandakumar, Reddy, Maryada Venkata Rami, Rathaur, Sushma (2006). Vaccination with <i>Setaria cervi</i> 175 kDa collagenase induces high level of protection against <i>Brugia malayi</i> infection in jirds . Vaccine.

Pokharel, D.R., Rai, R., Kumar, P., Chaturvedi, C.M., Rathaur, S.(2006). Tissue localization of collagenase and leucine aminopeptidase in the bovine filarial parasite Setaria cervi . Filaria Journal. 5.

Srivastava, Y, Gupta, S, Pokharel, DR, Rathaur, S (2004). <i>Setaria cervi</i> collagenase:: IgG cleavage and inhibition by <i>Wuchereria bancrofti</i> infected sera . Acta Parasitologica.

Srivastava, Y., Gupta, S., Pokharel, D.R., Rathaur, S.(2004). Setaria cervi collagenase: IgG cleavage and inhibition by Wuchereria bancrofti infected sera . Acta Parasitologica. 49. (3). p. 253-259.

(2004). Srivastava Y, Gupta S, Pokharel DR, Rathaur S. (2004). Setaria cervi collagenase: IgG cleavage and inhibition by Wuchereria bancrofti infected sera. Acta Parasitologica.

(2000). Tuladhar SM, Bajracharya GB, Pokharel DR, Giri R. (2000). The potentially anticarcinogenic flavonoids in vegetables, fruits and spices.

Kumar R, Adhikari RK, Sharma MK, Pokharel DR, Gautam N (2009) Pattern of ocular malignant tumors in Bhairahwa, Nepal . The Internet Journal of Ophthalmology and Visual Science.

OTHER

Kathayat, G., Pokharel, D.R., Yadav, N.K., Sigdel, M.(2021). Establishment of dry chemistry based reference intervals of renal function test parameters for the adult population of Kaski District, Nepal . Research Square.

DISSERTATION THESIS

(2010). Proteolytic enzymes of Filarial Parasite: Their Biochemical characterization and Vaccine Potential.