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Hire Dr. Dan S.

Profile Summary

I am a motivated and collaborative life scientist with a passion for communicating science. I have a PhD in molecular embryology, >6 years postdoctoral experience in stem cell biology and pre-clinical disease models, and a keen interest in the translation of basic science through innovation and multidisciplinary partnerships. I have a meticulous approach and consider myself an excellent problem solver and implementer. In addition to writing numerous primary research papers, I have thoroughly enjoyed writing commissioned review articles for Trends in Molecular Medicine and Nature Reviews Cancer. I lead projects by example and my enthusiasm, interpersonal skills and energy turn hard work into results. I can perform under pressure at the highest level and have a calm but tenacious approach to challenges. I have been involved in a wide range of research projects, including: - Engineering stem cells to secrete a variety of anti-tumour therapeutic proteins - Developing clinically-relevant cancer models in mice to test the efficacy of therapeutic stem cells - Creating a variety of FRET probes to record signalling events non-invasively in small animals - Made an inducible knock-in mouse line to genetically ablate a signalling centre in utero to establish its role in axis formation - Used confocal microscopy and culture techniques to localise patterns of proliferation in peri-implantation embryos - Defined the induction of antigen-specific allograft tolerance by intranasal peptide administration in skin transplant models - Established that Protein Kinase Cα is retained at the plasma membrane by the multidrug transporter P-glycoprotein

Subject Matter Expertise

• ☆ Molecular Imaging
• ☆ Peptide Synthesis
• ☆ Biology/Life Sciences
• ☆ Developmental Biology
• ☆ Embryology
• ☆ Animal Genetics
• ☆ Biochemistry
• ☆ Molecular Biology
• ☆ Kinases
• ☆ Stem Cell Research
• ☆ Microscopy
• ☆ Technical & Scientific Writing

Services

• Writing
• Consulting
• Product Development

Work Experience

Senior Postdoctoral Research Associate

Massachusetts General Hospital, Harvard Medical School

February 2012 - January 2015

Postdoctoral Research Associate

Imperial College London

March 2009 - January 2012

MRes/PhD Studentship

MRC Clinical Sciences Centre, Imperial College London

September 2003 - December 2008

Undergraduate Research Scientist

University of Illinois, Chicago

April 2002 - September 2002

Undergraduate Research Scientist

Duke University, North Carolina

April 2001 - September 2001

Education

PhD, Molecular Embryology

MRC Clinical Sciences Centre, Imperial College London

September 2004 - August 2008

Master of Research, Integrative Biomedical Science (Distinction)

Imperial College London

September 2003 - July 2004

Undergraduate Master of Biochemistry (First Class Honours)

University of Bath

September 1999 - June 2003

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Stuckey, Daniel W and Hingtgen, Shawn D and Karakas, Nihal and Rich, Benjamin E and Shah, Khalid(2015). Engineering Toxin-Resistant Therapeutic Stem Cells to Treat Brain Tumors. STEM CELLS. 33. (2). p. 589--600. Wiley Online Library

Stuckey, Daniel W and Shah, Khalid(2014). Stem cell-based therapies for cancer treatment: separating hope from hype. Nature Reviews Cancer. Nature Publishing Group

Stuckey, Daniel W and Shah, Khalid(2013). TRAIL on trial: preclinical advances in cancer therapy. Trends in molecular medicine. 19. (11). p. 685--694. Elsevier

Sardini, Alessandro and Stuckey, Daniel W and McGinty, James and Laine, Romain and Soloviev, Vadim Y and Arridge, Simon R and Wells, Dominic J and French, Paul MW and Hajnal, Joseph V(2012). In Vivo Investigation of Calpain Activity by Lifetime Imaging of Genetically Encoded FRET Sensors. Biophysical Journal. 102. (3). p. 159a. Cell Press

Laine, Romain and Stuckey, Daniel W and Manning, Hugh and Warren, Sean C and Kennedy, Gordon and Carling, David and Dunsby, Chris and Sardini, Alessandro and French, Paul MW(2012). Fluorescence lifetime readouts of troponin-c-based calcium fret sensors: a quantitative comparison of cfp and mtfp1 as donor fluorophores. PloS one. 7. (11). p. e49200. Public Library of Science

McGinty, James and Stuckey, Daniel W and Soloviev, Vadim Y and Laine, Romain and Wylezinska-Arridge, Marzena and Wells, Dominic J and Arridge, Simon R and French, Paul MW and Hajnal, Joseph V and Sardini, Alessandro(2011). In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse. Biomedical optics express. 2. (7). p. 1907--1917. Optical Society of America

Derbyshire, Katy and Addey, Caroline and Coe, David and Stuckey, Daniel W and Muezzin, Hasan and Bubier, Jason A and Shaffer, Daniel J and Roopenian, Derry C and Chai, Jian-Guo and Scott, Diane M(2011). Molecular mechanisms of induction of antigen-specific allograft tolerance by intranasal peptide administration. The Journal of Immunology. 186. (10). p. 5719--5728. Am Assoc Immnol

Kumar, Sunil and Alibhai, Dominic and Margineanu, Anca and Laine, Romain and Kennedy, Gordon and McGinty, James and Warren, Sean and Kelly, Douglas and Alexandrov, Yuriy and Munro, Ian and others(2011). FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ. ChemPhysChem. 12. (3). p. 609--626. Wiley Online Library

Soloviev, Vadim Y and McGinty, James and Stuckey, Daniel W and Laine, Romain and Wylezinska-Arridge, Marzena and Wells, Dominic J and Sardini, Alessandro and Hajnal, Joseph V and French, Paul MW and Arridge, Simon R(2011). Förster resonance energy transfer imaging in vivo with approximated radiative transfer equation. Applied optics. 50. (36). p. 6583--6590. Optical Society of America

Stuckey, Daniel W and Clements, Melanie and Di-Gregorio, Aida and Senner, Claire E and Le Tissier, Paul and Srinivas, Shankar and Rodriguez, Tristan A(2011). Coordination of cell proliferation and anterior-posterior axis establishment in the mouse embryo. Development. 138. (8). p. 1521--1530. The Company of Biologists Limited

Stuckey, Daniel W and Di Gregorio, Aida and Clements, Melanie and Rodriguez, Tristan A(2011). Correct patterning of the primitive streak requires the anterior visceral endoderm. PloS one. 6. (3). p. e17620. Public Library of Science

McGinty, James and Soloviev, Vadim Y and Tahir, Khadija B and Laine, Romain and Stuckey, Daniel W and Hajnal, Joseph V and Sardini, Alessandro and French, Paul MW and Arridge, Simon R(2009). Three-dimensional imaging of Förster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging. Optics letters. 34. (18). p. 2772--2774. Optical Society of America

Higgins, CF and Linton, KJ and Zolnerciks, JK and Stuckey, DW and Carmena, D and Sardini, A(2007). Protein Kinase C$α$ is retained at the plasma membrane by the multidrug transporter P-glycoprotein. Proceedings of The Physiological Society. The Physiological Society

Di-Gregorio, Aida and Sancho, Margarida and Stuckey, Daniel W and Crompton, Lucy A and Godwin, Jonathan and Mishina, Yuji and Rodriguez, Tristan A(2007). BMP signalling inhibits premature neural differentiation in the mouse embryo. Development. 134. (18). p. 3359--3369. The Company of Biologists Limited

CONFERENCE PAPER

McGinty, James and Stuckey, Daniel and Laine, Romain and Tahir, Khadija B and Neil, Mark A and Hajnal, Jo V and Sardini, Alex and French, Paul M(2010). Time-Domain Fluorescence Lifetime Optical Projection Tomography. Digital Holography and Three-Dimensional Imaging. p. DTuB7.

Soloviev, Vadim Y and Mohan, Surya P and Arridge, Simon R and McGinty, James and Laine, Romain and French, Paul M and Stuckey, Daniel W and Sardini, Alessandro and Hajnal, Joseph V(2010). Förster Resonance Energy Transfer Reconstruction from Optical Backprojections in Turbid Media. Biomedical Optics. p. BTuF4.

Soloviev, Vadim Y and McGinty, James and Tahir, Khadija B and Laine, Romain and Stuckey, Daniel W and Mohan, P Surya and Hajnal, Joseph V and Sardini, Alessandro and French, Paul MW and Arridge, Simon R(2010). Tomographic imaging of flourescence resonance energy transfer in highly light scattering media. BiOS. p. 75730G--75730G.

McGinty, James and Stuckey, Daniel W and Tahir, Khadija B and Laine, Romain and Hajnal, Joseph V and Sardini, Alessandro and French, Paul MW(2010). tomoFLIM-fluorescence lifetime projection tomography. BiOS. p. 75700G--75700G.

DISSERTATION THESIS

Stuckey, Daniel William(2008). Analysing the role of the anterior visceral endoderm in anterior-posterior axis establishment in the mouse embryo.