Profile Details

Create Project

★★★★★

☆☆☆☆☆

USD 95 /hr

★★★★★

☆☆☆☆☆

Hire Dr. Darío F.

Spain

USD 95 /hr

Expert in molecular biology & biochemistry | PhD | Scientific advisor in biotech, diagnostics & biomedical R&D

Profile Summary

I am a Molecular Biologist and PhD in Biochemistry with over 10 years of research experience in academia and translational biomedical science. I have led and collaborated on projects involving molecular mechanisms, gene expression, signaling pathways, diagnostics, and personalized medicine. My work has focused on the study of actin and myosin in reproductive biology, tumor cell cycle analysis, and plant-derived bioactive molecules with anticancer properties. I have also developed 3D printed models from medical images for teaching and clinical applications. I have worked at top-level research institutions including CONICET (Argentina), CSIC (Spain), and CIBERER, and I am currently a senior researcher and academic director at the University of Burgos (Spain), where I also lead innovation projects that combine biology, biotechnology, and digital tools. I offer scientific consulting in biotechnology, biomedical R&D, diagnostic technologies, academic writing, experimental design, and project evaluation. I am also a reviewer and member of ethics and research committees, with strong communication skills and a commitment to bridging science and industry.

Subject Matter Expertise

• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Structural Biology
• ☆ Apoptosis
• ☆ Oxidative Stress & Mitochondrial Function
• ☆ Antioxidants
• ☆ Enzyme Activity Assays
• ☆ Binding Assays
• ☆ Molecular Biology
• ☆ Biomarkers
• ☆ PCR
• ☆ Molecular Cloning
• ☆ DNA Sequencing
• ☆ Protein Sequencing
• ☆ Gene Expression Analysis
• ☆ Mutagenesis
• ☆ RNA Sequencing
• ☆ Plasmid DNA
• ☆ DNA Damage & Repair
• ☆ Kinases
• ☆ Monoclonal Antibody Production
• ☆ Cell Biology
• ☆ Cell-Based Assays
• ☆ Cytochemistry
• ☆ Cytogenetics
• ☆ Cell Signaling Pathways
• ☆ Membrane Biology
• ☆ Gene Regulation
• ☆ Stem Cell Research
• ☆ Biomarker Discovery
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Cell Banking
• ☆ Cell Line Engineering
• ☆ Cell Surface Receptors
• ☆ 3D Cell Culture
• ☆ Cytometry
• ☆ Cell Aging
• ☆ Device Fabrication
• ☆ 3D Printing/Additive Manufacturing

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing General Proofreading & Editing, Translation

Research User Research, Scientific and Technical Research, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting, Regulatory Consulting

Data & AI Statistical Analysis, Image Processing, Image Analysis, Data Visualization

Product Development Recipe Development, Product Evaluation, Prototyping, Device Fabrication

Work Experience

Chairman of the Editorial Board

Universidad de Burgos

March 2022 - Present

vice-dean

Universidad de Burgos

July 2021 - Present

Senior Distinguished Researcher "Beatriz Galindo"

Universidad de Burgos

February 2020 - Present

Scientific Researcher

National Scientific and Technical Research Council

June 2012 - January 2023

Full Professor

National University of the Northeast. School of Dentistry

December 2017 - January 2020

Researcher

National Scientific and Technical Research Council

June 2012 - November 2017

Posdoctoral Fellow

National Scientific and Technical Research Council

March 2012 - May 2012

Researcher

Centre for Biomedical Network Research on Rare Diseases

September 2009 - December 2011

PhD student

Spanish National Research Council

September 2004 - August 2009

PhD student

National Scientific and Technical Research Council

March 2001 - August 2004

Education

PhD. Biochemistry and Molecular Biology (Department of Biochemistry and Molecular Biology)

Complutense University of Madrid

September 2006 - June 2011

PhD. Biochemistry (School of Chemistry Biochemistry and Pharmacy)

National University of San Luis

March 2004 - November 2008

MSc. Biochemistry and Molecular Biology (Department of Biochemistry and Molecular Biology)

Complutense University of Madrid

September 2006 - June 2008

Degree in Molecular Biology (School of Chemistry Biochemistry and Pharmacy)

National University of San Luis

March 1996 - December 2003

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Malena Pérez-Sevilla, Fernando Rivas-Navazo, Pedro Latorre-Carmona, Darío Fernández-Zoppino (2025). Protocol for Converting DICOM Files to STL Models Using 3D Slicer and Ultimaker Cura . Journal of Personalized Medicine.

Malena Pérez Sevilla, Fernando Rivas Navazo, Pedro Latorre Carmona, Fernández, D; Fernández Zoppino, D (2025). Protocol for Converting DICOM Files to STL Models Using 3D Slicer and Ultimaker Cura . Journal of Personalized Medicine.

Carla Hernando-Muñoz, Andrea Revilla-Cuesta, Irene Abajo-Cuadrado, Camilla Andreini, Tomás Torroba, Natalia Busto, Darío Fernández, German Perdomo, Gerardo Acosta, Miriam Royo, et al. (2025). Self-assembling Depsipeptides on Aggregation-Induced Emission Luminogens: A New Way to Create Programmable Nanovesicles and Soft Nanocarriers . ACS Applied Materials & Interfaces.

Abi K. Funes, Virginia Avena, Paola V. Boarelli, María A. Monclus, Dario Fernández Zoppino, T Estefanía Saez Lancellotti, Miguel Fornés (2024). Cholesterol dynamics in rabbit liver: High-fat diet, olive oil, and synergistic dietary effects . Biochemical and Biophysical Research Communications.

Natalia Fern{\'{a}}ndez-Pamp{\'{\i}}n and M{\'{o}}nica Vaquero and Tania Gil and Gustavo Espino and Dar{\'{\i}}o Fern{\'{a}}ndez and Bego{\~{n}}a Garc{\'{\i}}a and Natalia Busto(2022). Distinct mechanism of action for antitumoral neutral cyclometalated Pt(II)-complexes bearing antifungal imidazolyl-based drugs . Journal of Inorganic Biochemistry. 226. p. 111663. Elsevier {BV}

Frank Lizaraso-Soto and Eduardo Guti{\'{e}}rrez-Abej{\'{o}}n and Juan Bustamante-Munguira and D{\'{e}}bora Mart{\'{\i}}n-Garc{\'{\i}}a and Mar{\'{\i}}a Montserrat Chimeno and {\'{A}}lvaro Nava-Rebollo and {\'{A}}lvaro Maurtua-Brise{\~{n}}o-Meiggs and Dar{\'{\i}}o Fern{\'{a}}ndez-Zoppino and Elena Bustamante-Munguira and F{\'{e}}lix Jes{\'{u}}s de Paz and Jes{\'{u}}s Grande-Villoria and Carlos Ochoa-Sangrador and Manuel Pascual and F. Javier {\'{A}}lvarez and Francisco Herrera-G{\'{o}}mez(2021). Binding Potassium to Improve Treatment With Renin-Angiotensin-Aldosterone System Inhibitors: Results From Multiple One-Stage Pairwise and Network Meta-Analyses of Clinical Trials . Frontiers in Medicine. 8. Frontiers Media {SA}

Ana Merino-Merino, Jeronimo Gonzalez-Bernal, Dario Fernandez-Zoppino, Ruth Saez-Maleta, Jose-Angel Perez-Rivera(2021). The Role of Galectin-3 and ST2 in Cardiology: A Short Review . Biomolecules. 11. (8). p. 1167. {MDPI} {AG}

Diego Fern{\'{a}}ndez-L{\'{a}}zaro and David Gallego-Gallego and Luis A. Corchete and Dar{\'{\i}}o Fern{\'{a}}ndez Zoppino and Jer{\'{o}}nimo J. Gonz{\'{a}}lez-Bernal and Blanca Garc{\'{\i}}a G{\'{o}}mez and Juan Mielgo-Ayuso(2021). Inspiratory Muscle Training Program Using the PowerBreath®: Does It Have Ergogenic Potential for Respiratory and/or Athletic Performance? A Systematic Review with Meta-Analysis . International Journal of Environmental Research and Public Health. 18. (13). p. 6703. {MDPI} {AG}

(2021). Athletic, muscular and hormonal evaluation in CrossFit  athletes using the “Elevation Training Mask” . Arch Med Deporte.

JG. Becchio and RO. Rosende and JE. Monz{\'{o}}n and D. Fern{\'{a}}ndez and PB. Said-Rücker(2021). Ultrasonic techniques to obtain dental pulp from impacted third molars . Journal of Clinical and Experimental Dentistry. p. e1--e7. Medicina Oral, S.L.

(2019). Enhancing survival, engraftment, and osteogenic potential of mesenchymal stem cells . World Journal of Stem Cells.

Said Rücker PB, Rosende RO, Krupp S, Becchio JG, Fernández D (2018) Functional Approaches to Oral-Maxillo-facial Restoration. Dentistry 8: 499. Doi: 10.4172/2161-1122.1000499 .

Costantino, V.V., Lobos-Gonzalez, L., Ibañez, J., Fernandez, D., Cuello-Carrión, F.D., Valenzuela, M.A., Barbieri, M.A., Semino, S.N., Jahn, G.A., Quest, A.F.G., et al.(2016). Dehydroleucodine inhibits tumor growth in a preclinical melanoma model by inducing cell cycle arrest, senescence and apoptosis . Cancer Letters. 372. (1). p. 10-23.

Fernández, D., Horrillo, A., Alquezar, C., González-Manchón, C., Parrilla, R., Ayuso, M.S.(2013). Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42 . Biochemical and Biophysical Research Communications. 432. (2). p. 302-307.

Losinno, A.D., Morales, A., Fernández, D., Lopez, L.A.(2012). Peritubular myoid cells from rat seminiferous tubules contain actin and myosin filaments distributed in two independent layers . Biology of Reproduction. 86. (5).

Pericacho, M., Alonso-Martín, S., Larrucea, S., González-Manchón, C., Fernández, D., Sánchez, I., Ayuso, M.S., Parrilla, R.(2011). Diminished thrombogenic responses by deletion of the Podocalyxin gene in mouse megakaryocytes . PLoS ONE. 6. (10).

Nowakowski, A., Alonso-Martín, S., Arias-Salgado, E.G., Fernández, D., Vilar, M., Ayuso, M.S., Parrilla, R.(2011). Megakaryocyte gene targeting mediated by restricted expression of recombinase Cre . Thrombosis and Haemostasis. 105. (1). p. 138-144.

Fernández, D., Larrucea, S., Nowakowski, A., Pericacho, M., Parrilla, R., Ayuso, M.S.(2011). Release of podocalyxin into the extracellular space. Role of metalloproteinases . Biochimica et Biophysica Acta - Molecular Cell Research. 1813. (8). p. 1504-1510.

Alonso-Martin, S., Nowakowski, A., Larrucea, S., Fernández, D., Vilar-Egea, M., Ayuso, M.S., Parrilla, R.(2010). Overexpression of podocalyxin in megakaryocytes and platelets decreases the bleeding time and enhances the agonist-induced aggregation of platelets . Thrombosis Research. 125. (6).

Nowakowski, A., Alonso-Martín, S., González-Manchón, C., Larrucea, S., Fernández, D., Vilar, M., Cerdán, S., Ayuso, M.S., Parrilla, R.(2010). Ventricular enlargement associated with the panneural ablation of the podocalyxin gene . Molecular and Cellular Neuroscience. 43. (1). p. 90-97.

Fernández, D., Bertoldi, M.V., Gómez, L., Morales, A., Callegari, E., Lopez, L.A.(2008). Identification and characterization of myosin from rat testicular peritubular myoid cells . Biology of Reproduction. 79. (6). p. 1210-1218.

Arias-Salgado, E.G., Larrucea, S., Butta, N., Fernández, D., García-Muñoz, S., Parrilla, R., Ayuso, M.S.(2008). Variations in platelet protein associated with arterial thrombosis . Thrombosis Research. 122. (5). p. 640-647.

Cruzado M, Castro C, Fernandez D, Gomez L, Roque M, Giordano OE, Lopez LA(2005). Dehydroleucodine inhibits vascular smooth muscle cell proliferation in G2 phase . Cellular and molecular biology (Noisy-le-Grand, France).

Cruzado, M., Castro, C., Fernández, D., Gomez, L., Roqué, M., Giordano, O.E., Lopez, L.A.(2005). Dehydroleucodine inhibits vascular smooth muscle cell proliferation in G2 phase . Cellular and Molecular Biology. 51. (6). p. 525-530.

Gamarra-Luques, C., Polo, M., Fernández, D., Arenas, G.N., Salomón, C.(2003). Effect of the coinfection with Escherichia coli on Leishmania sp phagocytosis in macrophage,Efecto de la coinfeccíon con E. coli sobre la fagocitosis de Leishmanias sp. en macrófagos . Prensa Medica Argentina. 90. (3). p. 248-253.

BOOK

Raúl Horacio Lucero, Darío Fernández Zoppino, Juan Estanislao Camarasa (2025). Cronista de una larga y silenciosa tragedia argentina .

PREPRINT

Peritubular macrophages phagocyte remains of undifferentiated spermatogonia in mouse testis <head> <META HTTP-EQUIV="Refresh" CONTENT="0;URL=/servlet/useragent"> </head> .

DISSERTATION THESIS

Fern\'andez, Dar\'\\io(2011). Caracterización, localización celular e interacción con otras proteínas de la podocalicina humana expresada en sistemas heterólogos.

Fern\'andez, Dar\'\\io(2008). Características de contracción de células mioides peritubulares del túbulo seminifero de testículo de rata.