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USD 60 /hr

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Hire Dr. David Z.

Australia

USD 60 /hr

Custom Protein Construct Design | From Sequence to Order-Ready Plasmid for CRO

Profile Summary

I am an experienced protein construct design specialist with a PhD structural biology and a strong track record of supporting biotech companies, research laboratories, and institutes worldwide. I focus on transforming raw protein sequences into optimized, expression-ready constructs that are practical, reliable, and tailored to diverse applications. My expertise includes domain boundary analysis, tag and cleavage site strategy, secretion signals, linker design, and codon optimization—delivering order-ready sequences with clear rationale and alternative variants to de-risk expression. Over the years, I have helped many teams accelerate projects and reduce trial-and-error by providing precisely designed constructs ready for synthesis and cloning. Clients value my ability to combine deep technical knowledge with practical insight, ensuring fast, professional, and effective solutions for complex protein design challenges.

Subject Matter Expertise

• ☆ Computational Biology
• ☆ Biochemistry
• ☆ Structural Biology
• ☆ Cell Biology
• ☆ Cell Culture

Services

• Consulting

Consulting Scientific and Technical Consulting

Work Experience

Project Leader

Walter and Eliza Hall Institute of Medical Research

May 2023 - Present

Education

PhD

Melbourne University

August 2017 - March 2021

Certifications

• Specialist in Molecular Biology

  Melbourne University

  March 2021 - Present

Publications

JOURNAL ARTICLE

William H. Palmer, Laura Ann Leaton, Ana Campos Codo, Bergren Crute, James Roest, Shiying Zhu, Jan Petersen, Richard P. Tobin, Patrick S. Hume, Matthew Stone, et al.(2023). Polymorphic KIR3DL3 expression modulates tissue-resident and innate-like T cells . Science Immunology. 8. (84). American Association for the Advancement of Science ({AAAS})

Shiying Zhu, Ehsan Kachooei, Jeffrey R. Harmer, Louise J. Brown, Frances Separovic, Marc-Antoine Sani(2021). TOAC spin-labeled peptides tailored for DNP-NMR studies in lipid membrane environments . Biophysical Journal. 120. (20). p. 4501--4511. Elsevier {BV}

Shiying Zhu, Daniel K. Weber, Frances Separovic, Marc‐Antoine Sani(2021). Expression and purification of the native C‐amidated antimicrobial peptide maculatin 1.1 . Journal of Peptide Science. 27. (8). Wiley

Marc-Antoine Sani, Shiying Zhu, Vinzenz Hofferek, Frances Separovic(2019). Nitroxide spin–labeled peptides for DNP‐NMR in‐cell studies . The FASEB Journal. 33. (10). p. 11021--11027. Wiley

Sarah A. Overall, Shiying Zhu, Eric Hanssen, Frances Separovic, Marc-Antoine Sani(2019). In Situ Monitoring of Bacteria under Antimicrobial Stress Using 31P Solid-State NMR . International Journal of Molecular Sciences. 20. (1). p. 181. {MDPI} {AG}

Shiying Zhu, Marc‐Antoine Sani, Frances Separovic(2018). Interaction of cationic antimicrobial peptides from Australian frogs with lipid membranes . Peptide Science. 110. (3). Wiley

Ting-Zheng Xie, Yuchen Yao, Xinyu Sun, Kevin J. Endres, Shiying Zhu, Xiaolei Wu, Hui Li, James M. Ludlow III, Tianbo Liu, Min Gao, et al.(2018). Supramolecular arrays by the self-assembly of terpyridine-based monomers with transition metal ions . Dalton Transactions. 47. (22). p. 7528--7533. Royal Society of Chemistry ({RSC})

Yuchen Yao, Sourav Chakraborty, Shiying Zhu, Kevin J. Endres, Ting-Zheng Xie, Wei Hong, Erendra Manandhar, Charles N. Moorefield, Chrys Wesdemiotis, George R. Newkome(2017). Stepwise, multicomponent assembly of a molecular trapezoid possessing three different metals . Chemical Communications. 53. (57). p. 8038--8041. Royal Society of Chemistry ({RSC})

Junwei Ding, Shiying Zhu, Tao Zhu, Wei Sun, Qing Li, Gang Wei, Zhiqiang Su(2015). Hydrothermal synthesis of zinc oxide-reduced graphene oxide nanocomposites for an electrochemical hydrazine sensor . RSC Advances. 5. (29). p. 22935--22942. Royal Society of Chemistry ({RSC})