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USD 100 /hr

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Hire Dr. Chaitanya D.

United States

USD 100 /hr

Flow Cytometry Consultant | Spectral Flow (Cytek Aurora) | Panel Design, Data analysis & Troubleshooting (FlowJo)

Profile Summary

I help academic labs and biotech teams fix complex flow cytometry problems from designing robust panels to rescuing confusing datasets and strengthening flow cytometry figures for grants and manuscripts. The goal is simple: clean, defensible results delivered faster, without trial-and-error. I am an Assistant Professor at UT Southwestern Medical Center with leadership experience in flow cytometry core operations and extensive hands-on expertise in spectral flow cytometry (Cytek Aurora). I bring a reviewer’s perspective to every project, helping clients proactively address issues related to controls, data quality, and reproducibility before submission. How I Can Help Flow Panel Design & Optimization Design and optimization of spectral and conventional flow panels, including fluorophore selection, titration strategies, and spillover/spread control. Deliverables: panel map, control plan (single-stain and FMO), titration worksheet, SOP, and gating logic. Flow Cytometry Troubleshooting (“Rescue My Data”) Diagnosis of staining issues, unmixing/compensation problems, viability artifacts, sample preparation pitfalls, and gating errors. Deliverables: annotated FlowJo workspace, written diagnosis, and a prioritized fix list. Grant & Manuscript Flow Review NIH-style review of flow cytometry figures, controls, and methods with reviewer-proof recommendations. Deliverables: marked-up figures and methods, control checklist, and response-ready suggestions. Technical Focus • Spectral flow (Cytek Aurora): reference controls, autofluorescence handling, unmixing QC • Control strategy: single stains, FMOs, dump channels, viability dyes, fixation/permeabilization planning • Analysis: FlowJo, gating optimization, reproducibility and reporting best practices • Immunology: myeloid and intestinal immune populations; T-cell subsets and B cells Engagement Remote consulting (Zoom + data review). Project-based or hourly. To get started, send a brief description of your question, panel/workspace, and timeline for a fast fit check and clear next steps.

Subject Matter Expertise

• ★ Cell Biology
• ★ Basic Immunology
• ★ Cytometry
• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Allergy & Clinical Immunology
• ☆ Clinical Immunology

Services

• Consulting

Consulting Scientific and Technical Consulting

Work Experience

ASSISTANT PROFESSOR

UT Southwestern Medical Center

September 2024 - Present

Assistant Professor/ Flow Core Director

The University of Texas Southwestern Medical Center

September 2024 - Present

Instrucor

UT Southwestern Medical Center

September 2021 - August 2024

Instructor

The University of Texas Southwestern Medical Center

September 2021 - August 2024

POST DOCTORAL SCIENTIST

The University of Texas Southwestern Medical Center

April 2016 - August 2021

Education

Ph.D

Indian Institute of Science, Bangalore

August 2009 - January 2016

Certifications

• Postdoctoral Researcher

  UT Southwestern

  April 2016 - August 2021

Publications

JOURNAL ARTICLE

Chaitanya Dende, Yuhao Wang, Meng Wang, Jiaxin Chen, Yun Li, Zheng Kuang, Prithvi Raj, Gabriella Quinn, Zehan Hu, Tarun Srinivasan, et al. (2023). The gut microbiota reprograms intestinal lipid metabolism through long noncoding RNA Snhg9 . Science.

(2022). Macrophages regulate gastrointestinal motility through complement component 1q . BioRxiv.

(2019). Epithelial retinoic acid receptor β regulates serum amyloid A expression and vitamin A-dependent intestinal immunity . Proceedings of the National Academy of Sciences of the United States of America.

(2017). Nanocurcumin is superior to native curcumin in preventing degenerative changes in Experimental Cerebral Malaria . Scientific reports.

(2015). Simultaneously targeting inflammatory response and parasite sequestration in brain to treat Experimental Cerebral Malaria . Scientific reports.

(2012). Curcumin-arteether combination therapy of Plasmodium berghei-infected mice prevents recrudescence through immunomodulation . PloS one.

(2010). Protective effect of Phyllanthus niruri on alcohol and heated sunflower oil induced hyperlipidemia in Wistar rats . Toxicology mechanisms and methods.

PREPRINT

Zarek, C. (2022). Coinfection with Intestinal Parasite Expands Resident Macrophages and Impairs Control of Chronic Herpesvirus Infection. Biorxiv. https://doi.org/10.1101/2022.10.05.510926 .

Chaitanya Dende, Bo Zhang, Matthew Brock, Carlos Arana, Lora Hooper, Prithvi Raj (2020). Impact of bead-beating intensity on microbiome recovery in mouse and human stool:Optimization of DNA extraction .