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Hire Dr. Carlos Alexandre H.

Brazil

Biological writer with 10 years experience; especially on biophysics and biochemistry fields

Profile Summary

Postdoctoral Fellow Research Scientist at São Paulo State University (UNESP) developing studies of molecular biophysics with proteins from causative organisms of tropical neglected tropical diseases, such as leishmaniasis, Chagas disease and snakebite envenoming. Proficiency in several structural molecular biology techniques including protein crystallography, small angle x-ray scattering, dynamic light scattering, molecular modelling, molecular dynamics simulations, fluorescence and circular dichroism spectroscopies, isothermal titraion calorimetry and high-performance liquid chromatography. Experience in teaching also, acting as professor of technical schools and at university of quantitative analytical chemistry, biophysics and molecular biology disciplines.

Subject Matter Expertise

• ★ Biochemistry
• ★ Protein/Enzyme Engineering
• ☆ STEM
• ☆ Chemistry
• ☆ X-Ray Crystallography
• ☆ Spectroscopy
• ☆ Biology/Life Sciences
• ☆ Molecular Biology
• ☆ Bioinformatics

Services

• Writing
• Research

Writing Clinical Trial Documentation, Technical Writing, Newswriting

Research Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Work Experience

High School Teacher of Chemistry and Biology

Secretariat of Education of the State of São Paulo

February 2020 - Present

Postdoctoral researcher

Sao Paulo State University

October 2019 - June 2020

Postdoctoral researcher

École Normale Supérieure Paris-Saclay

October 2018 - September 2019

Postdoctoral researcher and postgraduate professor

São Paulo State University

January 2014 - September 2018

Substitute professor of physics and biophysics disciplines of undergraduate courses in medical physics, biology and medicine at São Paulo State University (UNESP)

São Paulo State University (UNESP)

March 2017 - July 2017

Professor of Quantitative Analytical Chemistry

Maria Vitória Technical School

October 2009 - June 2012

Professor of History, Geography and Biology

College Prep Course V de Junho

April 2003 - November 2006

Education

Postdoctoral (Biophysics and Bioinfomatics)

ENS - Paris Saclay

October 2018 - September 2019

PhD (Physics and Biophysics)

São Paulo State University (UNESP)

January 2010 - December 2013

Master's degree in Genetics (Physics and Biophysics)

São Paulo State University (UNESP)

October 2007 - September 2009

Graduation in Biological Sciences

São Paulo State University (UNESP)

March 2003 - July 2007

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2020). Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity . Toxicon: X.

(2020). A multi-approach analysis highlights the relevance of RPA-1 as a telomere end-binding protein (TEBP) in Leishmania amazonensis . Biochimica et Biophysica Acta (BBA) - General Subjects.

(2020). Nuclear export of replication protein A in the nonreplicative infective forms of Trypanosoma cruzi . FEBS Letters.

Carlos A.H. Fernandes, Arina M. Perez, Andrea C. Barros, Thiago R. Dreyer, Marcelo S. da Silva, Edna Gicela O. Morea, Marcos R.M. Fontes, Maria Isabel N. Cano(2019). Dual cellular localization of the Leishmania amazonensis Rbp38 ({LaRbp}38) explains its affinity for telomeric and mitochondrial {DNA} . Biochimie. 162. p. 15--25. Elsevier {BV}

(2019). Identification, description and structural analysis of beta phospholipase A2 inhibitors (sbβPLIs) from Latin American pit vipers indicate a binding site region for basic snake venom phospholipases A2. Toxicon X.

Zambelli, V.O. and Picolo, G. and Fernandes, C.A.H. and Fontes, M.R.M. and Cury, Y.(2017). Secreted phospholipases A2 from animal venoms in pain and analgesia . Toxins. 9. (12).

de Lima, L.F.G. and Borges, R.J. and Viviescas, M.A. and Fernandes, C.A.H. and Fontes, M.R.M.(2017). Structural studies with BnSP-7 reveal an atypical oligomeric conformation compared to phospholipases A2-like toxins . Biochimie. 142. p. 11-21.

Pavani, R.S., Vitarelli, M.O., Fernandes, C.A.H., Mattioli, F.F., Morone, M., Menezes, M.C., Fontes, M.R.M., Cano, M.I.N., Elias, M.C.(2017). Replication Protein A-1 Has a Preference for the Telomeric G-rich Sequence in Trypanosoma cruzi . Journal of Eukaryotic Microbiology.

Fernandes, C.A.H., Pazin, W.M., Dreyer, T.R., Bicev, R.N., Cavalcante, W.L.G., Fortes-Dias, C.L., Ito, A.S., Oliveira, C.L.P., Fernandez, R.M., Fontes, M.R.M.(2017). Biophysical studies suggest a new structural arrangement of crotoxin and provide insights into its toxic mechanism . Scientific Reports. 7.

Picelli, C.G. and Borges, R.J. and Fernandes, C.A.H. and Matioli, F.M. and Fernandes, C.F.C. and Sobrinho, J.C. and Holanda, R.J. and Ozaki, L.S. and Kayano, A.M. and Calderon, L.A. and Fontes, M.R.M. and St{\'a}beli, R.G. and Soares, A.M.(2017). Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes . International Journal of Biological Macromolecules. 103. p. 525-532.

Morea, E.G.O., Viviescas, M.A., Fernandes, C.A.H., Matioli, F.F., Lira, C.B.B., Fernandez, M.F., Morea, E.G.O., Viviescas, M.A., Fernandes, C.A.H., Matioli, F.F., et al.(2017). A calmodulin-like protein (LCALA) is a new Leishmania amazonensis candidate for telomere end-binding protein . Biochimica et Biophysica Acta - General Subjects. 1861. (11). p. 2583-2597.

Borges, R.J. and Cardoso, F.F. and Fernandes, C.A.H. and Dreyer, T.R. and de Moraes, D.S. and Floriano, R.S. and Rodrigues-Simioni, L. and Fontes, M.R.M.(2017). Functional and structural studies of a Phospholipase A2-like protein complexed to zinc ions: Insights on its myotoxicity and inhibition mechanism . Biochimica et Biophysica Acta - General Subjects. 1861. (1). p. 3199-3209.

Estev{\~a}o-Costa, M.I. and Fernandes, C.A.H. and Mudadu, M.D.A. and Franco, G.R. and Fontes, M.R.M. and Fortes-Dias, C.L.(2016). Structural and evolutionary insights into endogenous alpha-phospholipase A2 inhibitors of Latin American pit vipers . Toxicon. 112. p. 35-44.

Pavani, R.S., da Silva, M.S., Pavani, R.S., da Silva, M.S., Fernandes, C.A.H., Morini, F.S., Araujo, C.B., Fontes, M.R.D.M., Sant’Anna, O.A., Machado, C.R., et al.(2016). Replication Protein A Presents Canonical Functions and Is Also Involved in the Differentiation Capacity of Trypanosoma cruzi . PLoS Neglected Tropical Diseases. 10. (12).

Makiyama, R.K., Fernandes, C.A.H., Dreyer, T.R., Moda, B.S., Matioli, F.F., Fontes, M.R.M., Maia, I.G.(2016). Structural and thermodynamic studies of the tobacco calmodulin-like rgs-CaM protein . International Journal of Biological Macromolecules. 92. p. 1288-1297.

Fernandes, C.A.H. and Cardoso, F.F. and Cavalcante, W.G.L. and Soares, A.M. and Dal-Pai, M. and Gallacci, M. and Fontes, M.R.M.(2015). Structural basis for the inhibition of a phospholipase A2-like toxin by caffeic and aristolochic acids . PLoS ONE. 10. (7).

Guimarães, C.L.S. and Andrião-Escarso, S.H. and Moreira-Dill, L.S. and Carvalho, B.M.A. and Marchi-Salvador, D.P. and Santos-Filho, N.A. and Fernandes, C.A.H. and Fontes, M.R.M. and Giglio, J.R. and Barraviera, B. and Zuliani, J.P. and Fernandes, C.F.C. and Calderón, L.A. and Stábeli, R.G. and Albericio, F. and Da Silva, S.L. and Soares, A.M.(2014). Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2: A biotechnological tool to improve the production of antibodies . BioMed Research International. 2014.

Fernandes, C.A.H., Borges, R.J., Lomonte, B., Fontes, M.R.M.(2014). A structure-based proposal for a comprehensive myotoxic mechanism of phospholipase A2-like proteins from viperid snake venoms . Biochimica et Biophysica Acta - Proteins and Proteomics. 1844. (12). p. 2265-2276.

Fortes-Dias, C.L., Ortolani, P.L., Fernandes, C.A.H., Lobo, K.R., Melo, L.A.D., Borges, M.H., Pazin, W.M., Oliveira Neto, M.D., Fernandez, R.M., Fontes, M.R.M.(2014). Insights on the structure of native CNF, an endogenous phospholipase A 2 inhibitor from Crotalus durissus terrificus, the South American rattlesnake . Biochimica et Biophysica Acta - Proteins and Proteomics. 1844. (9). p. 1569-1579.

Pavani, R.S., Fernandes, C., Perez, A.M., Vasconcelos, E.J.R., Siqueira-Neto, J.L., Fontes, M.R., Cano, M.I.N.(2014). RPA-1 from Leishmania amazonensis (LaRPA-1) structurally differs from other eukaryote RPA-1 and interacts with telomeric DNA via its N-terminal OB-fold domain . FEBS Letters. 588. (24). p. 4740-4748.

Fortes-Dias, C.L. and Ortolani, P.L. and Fernandes, C.A.H. and Lobo, K.R. and Melo, L.A.D. and Borges, M.H. and Pazin, W.M. and Oliveira Neto, M.D. and Fernandez, R.M. and Fontes, M.R.M.(2014). Insights on the structure of native CNF, an endogenous phospholipase A2 inhibitor from Crotalus durissus terrificus, the South American rattlesnake . Biochimica et Biophysica Acta - Proteins and Proteomics. 1844. (9). p. 1569-1579.

Fernandes, C.A.H., Comparetti, E.J., Borges, R.J., Huancahuire-Vega, S., Ponce-Soto, L.A., Marangoni, S., Soares, A.M., Fontes, M.R.M., Fernandes, C.A.H., Fernandes, C.A.H., et al.(2013). Structural bases for a complete myotoxic mechanism: Crystal structures of two non-catalytic phospholipases A2-like from Bothrops brazili venom . Biochimica et Biophysica Acta - Proteins and Proteomics. 1834. (12). p. 2772-2781.

Takeda, A.A.S., Freitas, F.Z., Magro, A.J., Bernardes, N.E., Fernandes, C.A.H., Goncalves, R.D., Bertolini, M.C., Fontes, M.R.M.(2013). Biophysical characterization of the recombinant importin-α from neurospora crassa . Protein and Peptide Letters. 20. (1). p. 8-16.

Vieira, L.F., Magro, A.J., Fernandes, C.A.H., De Souza, B.M., Cavalcante, W.L.G., Palma, M.S., Rosa, J.C., Fuly, A.L., Fontes, M.R.M., Gallacci, M., et al.(2013). Biochemical, functional, structural and phylogenetic studies on Intercro, a new isoform phospholipase A2 from Crotalus durissus terrificus snake venom . Biochimie. 95. (12). p. 2365-2375.

Salvador, G.H.M., Fernandes, C.A.H., Magro, A.J., Marchi-Salvador, D.P., Cavalcante, W.L.G., Fernandez, R.M., Gallacci, M., Soares, A.M., Oliveira, C.L.P., Fontes, M.R.M.(2013). Structural and Phylogenetic Studies with MjTX-I Reveal a Multi-Oligomeric Toxin - a Novel Feature in Lys49-PLA2s Protein Class . PLoS ONE. 8. (4).

Fernandes, C.A.H., Gartuzo, E.C.G., Pagotto, I., Comparetti, E.J., Huancahuire-Vega, S., Ponce-Soto, L.A., Costa, T.R., Marangoni, S., Soares, A.M., Fontes, M.R.M.(2012). Crystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A2 from Bothrops brazili venom . Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 68. (8). p. 935-938.

Costa, T.R., Fernandes, C.A.H., Fontes, M.R.M., Magro, A.J., Shimabuku, P.S., Soares, A.M.(2011). Crystallization and preliminary X-ray diffraction analysis of a Lys49-phospholipase A 2 complexed with caffeic acid, a molecule with inhibitory properties against snake venoms . Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 67. (2). p. 249-252.

Dos Santos, J.I., Cintra-Francischinelli, M., Borges, R.J., Fernandes, C.A.H., Pizzo, P., Cintra, A.C.O., Braz, A.S.K., Soares, A.M., Fontes, M.R.M.(2011). Structural, functional, and bioinformatics studies reveal a new snake venom homologue phospholipase A 2 class . Proteins: Structure, Function and Bioinformatics. 79. (1). p. 61-78.

Estevão-Costa, M.I., Fernandes, C.A.H., Fontes, M.R.M., Fortes-Dias, C.L., Magro, A.J., Menaldo, D.L., Murakami, M.T., Santos, C.R., Santos-Filho, N.A., Soares, A.M.(2011). Molecular cloning and biochemical characterization of a myotoxin inhibitor from Bothrops alternatus snake plasma . Biochimie. 93. (3). p. 583-592.

Costa, T.R., Fernandes, C.A.H., Fontes, M.R.M., Marchi-Salvador, D.P., Salvador, G.M., Silva, M.C.O., Soares, A.M.(2010). Comparison between apo and complexed structures of bothropstoxin-I reveals the role of Lys122 and Ca2+-binding loop region for the catalytically inactive Lys49-PLA2s . Journal of Structural Biology. 171. (1). p. 31-43.

Alves, S.V., Bagnaresi, P., Budu, A., Bueno, V.B., Catalani, L.H., Fernandes, C.A., Fontes, M.R., Garcia, C.R.S., Netto, L.E., Oliveira, P.L., et al.(2010). In vivo uptake of a haem analogue Zn protoporphyrin IX by the human malaria parasite P. falciparum-infected red blood cells . Cell Biology International. 34. (8). p. 859-865.

Fernandes, C.A.H., Fontes, M.R.M., Magro, A.J., Santos, J.I.D., Fernandes, C.A.H., Fontes, M.R.M., Magro, A.J., Santos, J.I.D.(2009). Influence of quaternary conformation on the biological activities of the Asp49-phospholipases A2s from snake venoms . Protein and Peptide Letters. 16. (8). p. 852-859.

Fernandes, C.A.H., Fontes, M.R.M., Marchi-Salvador, D.P., Silveira, L.B., Soares, A.M.(2009). Crystal structure of a phospholipase A2 homolog complexed with p-bromophenacyl bromide reveals important structural changes associated with the inhibition of myotoxic activity . Biochimica et Biophysica Acta - Proteins and Proteomics. 1794. (11). p. 1583-1590.

Fernandes, C.A.H., Fontes, M.R.M., Magro, A.J., Santos, J.I.D.(2009). The intriguing phospholipases A2 homologues: Relevant structural features on myotoxicity and catalytic inactivity . Protein and Peptide Letters. 16. (8). p. 887-893.

Corra, L.C., Fernandes, C.A.H., Fontes, M.R.M., Salvador, G.H.M., Santos-Filho, N.A., Soares, A.M.(2009). Crystallization and preliminary X-ray diffraction analysis of crotoxin B from crotalus durissus collilineatus venom . Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 65. (10). p. 1011-1013.

Dos Santos, J.I., Fernandes, C.A.H., Fontes, M.R.M., Marchi-Salvador, D.P., Silveira, L.B., Soares, A.M.(2007). Preliminary X-ray crystallographic studies of a Lys49-phospholipase A 2 homologue from Bothrops pirajai venom complexed with p-bromophenacyl bromide and α-tocopherol inhibitors . Protein and Peptide Letters. 14. (7). p. 698-701.

Amui, S.F., Fernandes, C.A.H., Fontes, M.R.M., Marchi-Salvador, D.P., Soares, A.M.(2006). Crystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA2 from Bothrops jararacussu venom complexed with p-bromophenacyl bromide . Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 62. (6). p. 600-603.