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Hire Dr. Bhanu Prakash P.

Germany

Molecular Biologist | Cloning & Plasmid Design Consultant | 6+ Years’ Experience

Profile Summary

I am a molecular biologist with more than six years of hands-on experience in molecular cloning, plasmid design, plant overexpression constructs, CRISPR construct design, and bacterial expression constructs. My research background includes plant molecular biology, CRISPR/dCas9-based systems, fluorescent protein tagging, Golden Gate cloning, Gibson assembly, restriction cloning, guide RNA systems, and vector planning. During my PhD and research work in Germany, I designed and optimized cloning workflows for plant expression, CRISPR-based imaging, and protein expression applications. Through CloneWise, I support researchers, academic labs, and biotech teams with in silico cloning strategy, construct planning, plasmid design, vector selection, and review of cloning workflows before experimental work begins. My focus is to help clients choose the most suitable cloning strategy, identify design issues early, and move forward with a clearer experimental plan.

Subject Matter Expertise

• ☆ Molecular Biology
• ☆ Molecular Cloning
• ☆ Plasmid DNA
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Biomolecular Engineering
• ☆ Gene Editing/CRISPR
• ☆ Protein/Enzyme Engineering

Services

• Research
• Consulting

Research Scientific and Technical Research

Consulting Scientific and Technical Consulting

Work Experience

Postdoctoral researcher

Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung

November 2024 - Present

Postdoctoral Researcher

Leibniz Institute of Plant Genetics and Crop Plant Research

November 2024 - December 2025

PhD candidate

Leibniz Institute of Plant Genetics and Crop Plant Research

June 2021 - October 2024

Doctoral Candidate

Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)

October 2020 - October 2024

Research Assistant

Leibniz Institute of Plant Genetics and Crop Plant Research

April 2020 - May 2021

Scientific Assistant

Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)

April 2020 - September 2020

Education

Doctorate

Leibniz Institute of Plant Genetics and Crop Plant Research

June 2021 - October 2024

Masters in Crop Sciencies

University of Hohenheim

October 2017 - October 2020

Certifications

• Certification details not provided.

Publications

PREPRINT

Aurélien Devillars, Silvia Farinati, Adriana Fernanda Soria Garcia, Justin Joseph, Giovanni Gabelli, Sara Zenoni, Edoardo Bertini, Alessandra Amato, Bhanu Prakash Potlapalli, Andreas Houben, et al. (2026). GRASP: A PLANT TRANSFORMATION-INDEPENDENT CRISPR-BASED SYSTEM FOR AFFINITY PURIFICATION OF SPECIFIC CHROMATIN LOCI .

Bhanu Prakash Potlapalli, Fabian Dassau, Jörg Fuchs, Deboprio Roy Sushmoy, Andreas Houben (2025). CRISPR-CISH: An in situ chromogenic DNA repeat detection system for research and life science education .

JOURNAL ARTICLE

Bhanu Prakash Potlapalli, Fabian Dassau, Jörg Fuchs, Deboprio Roy Sushmoy, Andreas Houben (2025). CRISPR-CISH: an in situ chromogenic DNA repeat detection system for research and life science education . Chromosome Research.

Andreas Houben, Bhanu Prakash Potlapalli, Solmaz Khosravi(2024). Genomische Sequenzen sichtbar machen . Biologie in unserer Zeit - BiuZ. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString Bd. 54 Nr. 5 (2024): Biologie in unserer Zeit 5/24-Sonderheft CRISPR. Biologie in unserer Zeit - BiuZ

Bhanu Prakash Potlapalli, Jörg Fuchs, Twan Rutten, Armin Meister, Andreas Houben, James Murray (2024). The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit . Journal of Experimental Botany.

(2020). Application of Tris-HCl Allows the Specific Labeling of Regularly Prepared Chromosomes by CRISPR-FISH . Cytogenetic and Genome Research.

BOOK CHAPTER

Bhanu Prakash Potlapalli, Takayoshi Ishii, Kiyotaka Nagaki, Saravanakumar Somasundaram, Andreas Houben (2023). CRISPR-FISH: A CRISPR/Cas9-Based In Situ Labeling Method . Methods in Molecular Biology.