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PhD Researcher | Expert in Lignocellulosic Biorefinery, Biomass Valorization & Enzyme Production

Profile Summary

I am a PhD-level researcher with over five years of international experience across Mexico, Taiwan, and Chile, specializing in the sustainable conversion of biomass into high-value resources. My work is dedicated to the lignocellulosic biorefinery—finding the perfect balance between technical precision and environmental impact. My career has been built in diverse, world-class laboratories where I’ve mastered the complexities of hydrothermal pretreatment and fermentation technology. I don’t just see agricultural waste; I see the untapped potential for biofuels and industrial enzymes. Whether it's optimizing bioreactors or designing zero-waste systems, I am passionate about creating bioprocesses that are both efficient and scalable. Previous & Ongoing Research Projects: 1. Universidad Técnica Federico Santa María (July, 2023-July, 2025) Postdoctoral appointment concluded at Universidad Técnica Federico Santa María Project: Conducted biological pretreatment of wheat straw using white-rot fungi to enhance cellulase production; evaluated enzyme performance under solid-state fermentation conditions. 2. National Kaohsiung University of Science & Technology (May 10, 2022-Dec 29, 2022) Worked as a project research assistant at the National Kaohsiung University of Science & technology Project: Worked on the valorization of lignocellulosic biomass residues like sorghum distillery residue for bioethanol and other platform chemical production. This project includes hydrothermal pretreatment of the biomass and various bioprocess for value addition. 3. PhD Research (2018-2021) Project: My Ph.D. thesis work was focused on a comparative analysis of agave bagasse generated from different tequila-producing processes based on cellulose percentage by autohydrolysis pretreatment, enzymatic hydrolysis, and enzyme production by solid-state fermentation. Project 1: Characterization of different agave bagasse extracted from different tequila production methods before pretreatment and after pretreatment. Project 2: Pretreatment of agave bagasse at a large scale with selected optimized conditions and enzymatic hydrolysis with commercial enzyme Project 3: Using the best-pretreated agave bagasse out of three different agave bagasse as a carbon source in solid-state fermentation for lignocellulosic enzyme production. 4. Indian Oil Work (2015-2018) Worked on the development of fungal mutant strains for increased cellulase production, worked on upstream (Submerged fermentation) and downstream processing (clarification, precipitation, ultra-filtration, etc) of enzymes. Experience in handling stirred tank fermenters (1L to 7 L) and enzymatic saccharification. Education Details 2018- 2021 PhD, Autonomous University of Coahuila Thesis: Development of the Bioprocessing Technique for Lignocellulosic Enzyme Production. 2010-2012 MSc Biotechnology, Amity University, Noida, Uttar Pradesh Dissertation topic: Screening of microalgal mutants for higher growth and salt tolerance. 2007-2010 BSc Biotechnology, HIMT, Greater Noida, Uttar Pradesh/C.C.S.U Technical Skills • Solid‑state & Submerged Fermentation • Hydrothermal & Biological Pretreatments • Enzymatic Saccharification • Classical Mutation & Strain Improvement • Bioreactor Operations • Upstream & Downstream Processing • HPLC & Analytical Techniques

Subject Matter Expertise

• ☆ Enzyme Activity Assays
• ☆ Industrial Biotechnology

Services

• Writing
• Research
• Consulting
• Product Development

Writing Technical Writing, General Proofreading & Editing

Research Scientific and Technical Research

Consulting Scientific and Technical Consulting

Product Development Concept Development

Work Experience

Post Doctoral

Universidad Técnica Federico Santa María

July 2023 - July 2025

Project research assistant

National Kaohsiung University of Science and Technology

May 2022 - December 2022

Research Scholar

Autonomous University of Coahuila

July 2018 - July 2021

Project Assistant

Indian Oil Corporation Research and Development Centre

January 2015 - June 2018

Education

PhD

Autonomous University of Coahuila

August 2018 - February 2022

Certifications

• Not applicable

  NA

  January 2026 - January 2026

Publications

JOURNAL ARTICLE

Anusuiya Singh, Carolyn Palma Toloza, María Victoria Riquelme Muñoz, Andrea Carvajal Guevara(2025). Biological pretreatment of wheat straw using Trametes versicolor for enhanced cellulase production in solid-state fermentation . Bioprocess and biosystems engineering.

Anusuiya Singh, Carolyn Palma Toloza, Reeta Rani Singhania, Andrea Carvajal Guevara(2024). Hydrothermal pretreatment with microwaves: A new strategy for ensuring the sustainability of biorefineries . Energy & Environment.

Anusuiya Singh, Karla D. González-Gloria, Elia Tomás-Pejó, Lorena Amaya-Delgado, Rosa M. Rodríguez-Jasso, Araceli Loredo-Treviño, Meenu Hans, Carlos Martín, Sachin Kumar, Héctor A. Ruiz(2024). Biochemical and Biorefinery Platform for Second-Generation Bioethanol: Fermentative Strategies and Microorganisms . Fermentation.

Anusuiya Singh, Cheng-Di Dong, Ashutosh Tiwari, Grace Sathyanesan Anisha, Chiu-Wen Chen, Dibyajyoti Haldar, Anil Kumar Patel, Reeta Rani Singhania (2023). Laccase: A potential biocatalyst for pollutant degradation . Environmental Pollution.

Anusuiya Singh, Cheng-Di Dong, Ashutosh Tiwari, Grace Sathyanesan Anisha, Chiu-Wen Chen, Dibyajyoti Haldar, Anil Kumar Patel, Reeta Rani Singhania(2023). Laccase: A potential biocatalyst for pollutant degradation . Environmental pollution (Barking, Essex : 1987).

Anusuiya Singh, Mei-Ling Tsai, Chiu-Wen Chen, Reeta Rani Singhania, Anil Kumar Patel, Vaibhav Tambat, Cheng-Di Dong (2023). Role of hydrothermal pretreatment towards sustainable biorefinery . Bioresource Technology.

Anusuiya Singh, Chiu-Wen Chen, Anil Kumar Patel, Cheng-Di Dong, Reeta Rani Singhania(2022). Subcritical Water Pretreatment for the Efficient Valorization of Sorghum Distillery Residue for the Biorefinery Platform . Bioengineering (Basel, Switzerland).

Anusuiya Singh, Chiu-Wen Chen, Anil Patel, Cheng-Di Dong, Reeta Singhania (2022). Subcritical Water Pretreatment for the Efficient Valorization of Sorghum Distillery Residue for the Biorefinery Platform . Bioengineering.

Anusuiya Singh, Mei-Ling Tsai, Chiu-Wen Chen, Reeta Rani Singhania, Anil Kumar Patel, Vaibhav Tambat, Cheng-Di Dong(2022). Role of hydrothermal pretreatment towards sustainable biorefinery . Bioresource technology.

Anusuiya Singh, Reeta Rani Singhania, Shveta Soam, Chiu-Wen Chen, Dibyajyoti Haldar, Sunita Varjani, Jo-Shu Chang, Cheng-Di Dong, Anil Kumar Patel (2022). Production of bioethanol from food waste: Status and perspectives . Bioresource Technology.

Anusuiya Singh, Reeta Rani Singhania, Shveta Soam, Chiu-Wen Chen, Dibyajyoti Haldar, Sunita Varjani, Jo-Shu Chang, Cheng-Di Dong, Anil Kumar Patel(2022). Production of bioethanol from food waste: Status and perspectives . Bioresource technology.

Anusuiya Singh, Reeta Rani Singhania, Anil Kumar Patel, Dibyajyoti Haldar, Shveta Soam, Chiu-Wen Chen, Mei-Ling Tsai, Cheng-Di Dong (2022). Consolidated bioprocessing of lignocellulosic biomass: Technological advances and challenges . Bioresource Technology.

Anusuiya Singh, Reeta Rani Singhania, Anil Kumar Patel, Dibyajyoti Haldar, Shveta Soam, Chiu-Wen Chen, Mei-Ling Tsai, Cheng-Di Dong(2022). Consolidated bioprocessing of lignocellulosic biomass: Technological advances and challenges . Bioresource technology.

Anusuiya Singh, Rosa M. Rodríguez-Jasso, Rohit Saxena, Ruth Belmares Cerda, Reeta Rani Singhania, Héctor A. Ruiz(2021). Subcritical water pretreatment for agave bagasse fractionation from tequila production and enzymatic susceptibility . Bioresource technology.

Singh, A., Rodríguez-Jasso, R.M., Saxena, R., Cerda, R.B., Singhania, R.R., Ruiz, H.A.(2021). Subcritical water pretreatment for agave bagasse fractionation from tequila production and enzymatic susceptibility . Bioresource Technology. 338.

Anusuiya Singh, Edgar Olguin-Maciel, Rubi Chable-Villacis, Raul Tapia-Tussell, Héctor A. Ruiz(2020). Consolidated Bioprocessing, an Innovative Strategy towards Sustainability for Biofuels Production from Crop Residues: An Overview . Agronomy.

Olguin-Maciel, E., Singh, A., Chable-Villacis, R., Tapia-Tussell, R., Ruiz, H.A.(2020). Consolidated bioprocessing, an innovative strategy towards sustainability for biofuels production from crop residues: An overview . Agronomy. 10. (11).

Anusuiya Singh, Rosa M. Rodriguez Jasso, Karla D. Gonzalez-Gloria, Miriam Rosales, Ruth Belmares Cerda, Cristóbal N. Aguilar, Reeta Rani Singhania, Héctor A. Ruiz(2019). The enzyme biorefinery platform for advanced biofuels production . Bioresource Technology Reports.

Singh, A., Rodríguez Jasso, R.M., Gonzalez-Gloria, K.D., Rosales, M., Belmares Cerda, R., Aguilar, C.N., Singhania, R.R., Ruiz, H.A.(2019). The enzyme biorefinery platform for advanced biofuels production . Bioresource Technology Reports. 7.

Anusuiya Singh, Neha Vaishnav, Mukund Adsul, Pooja Dixit, Simranjeet Kaur Sandhu, Anshu S. Mathur, Suresh Kumar Puri, Reeta Rani Singhania(2018). Penicillium: The next emerging champion for cellulase production . Bioresource Technology Reports.

Vaishnav, N., Singh, A., Adsul, M., Dixit, P., Sandhu, S.K., Mathur, A., Puri, S.K., Singhania, R.R.(2018). Penicillium: The next emerging champion for cellulase production . Bioresource Technology Reports. 2. Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 131-140.

Anusuiya Singh, Anil Kumar Patel, Mukund Adsul, Anshu S. Mathur, Reeta Rani Singhania(2017). Genetic modification: a tool for enhancing cellulase secretion . Biofuel Research Journal.

Singh, A., Adsul, M., Vaishnav, N., Mathur, A., Singhania, R.R.(2017). Improved cellulase production by Penicillium janthinellum mutant . Indian Journal of Experimental Biology. 55. (7). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 436-440.

Singh, A., Patel, A.K., Adsul, M., Singhania, R.R.(2017). Genetic modification: A tool for enhancing cellulase secretion . Biofuel Research Journal. 4. (2). Microsoft.AspNetCore.Mvc.Localization.LocalizedHtmlString 600-610.

OTHER

Anusuiya Singh, Mukund Adsul, Neha Vaishnav, Anshu S. Mathur, Reeta Rani Singhania(2017). Improved cellulase production by Penicillium janthinellum mutant .