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Hire Dr. Anthony O.

United States

USD 15 /hr

Expert in Synaptic Function, Data Analysis, and Microscopy with 15+ Years of Experience

Profile Summary

As a neuroscientist with a focus on gene editing, I have worked on various research projects that have contributed to the understanding of neurodegenerative diseases and the role of specific proteins and enzymes in their pathogenesis. Here is a summary of my previous research and projects: Perturbations of the endolysosomal pathway in neurodegenerative diseases: I have extensively studied the role of VPS35 and the retromer complex in the endolysosomal system and their implications in Parkinson's disease (PD) and Alzheimer's disease (AD). My research has focused on the PD-linked D620N mutation in VPS35 and its impact on retromer dysfunction, as well as the connections between VPS35, the retromer, and other neurodegenerative diseases such as amyotrophic lateral sclerosis and primary tauopathies. Neuronal expression of Transglutaminase-2 (TG2) in rodent models of chronic-relapsing and non-relapsing EAE: My investigation has explored the expression of TG2 in subsets of macrophages, microglia, and astrocytes after experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). I have examined the neuronal pathology during disease progression and identified the induction of cytoplasmic TG2 in neurons surrounding perivascular lesion sites. Mitochondrial quality control and PTEN-induced kinase 1 (PINK1) in inflammatory lung injury and neurodegenerative diseases: My research has shown that the ubiquitin E3 ligase receptor component, FBXO7, targets PINK1 for cellular disposal, leading to mitochondrial injury and inflammation. I have identified a small molecule, BC1464, which disrupts the association between FBXO7 and PINK1, enhancing mitochondrial quality and limiting inflammatory injury. BC1464 has also demonstrated neuroprotective effects in various culture models of Parkinson's disease. My educational background and expertise in synaptic function, data analysis, and microscopy, as well as my experience in gene editing, have been instrumental in advancing the understanding of neurodegenerative diseases and their underlying mechanisms.

Subject Matter Expertise

• ★ Cell Biology
• ★ Neuroscience
• ★ Data Analysis
• ☆ Cell-Based Assays
• ☆ Cytochemistry
• ☆ Cell Signaling Pathways
• ☆ Gene Regulation
• ☆ Stem Cell Research
• ☆ Developmental Neuroscience
• ☆ Molecular Neuroscience
• ☆ Neurobiology
• ☆ Microscopy
• ☆ Cell Culture
• ☆ Primary Cells
• ☆ Cell Surface Receptors
• ☆ R
• ☆ Neurology
• ☆ Parkinson's Disease
• ☆ Alzheimer's Disease

Services

• Writing
• Research
• Consulting
• Data & AI

Writing Clinical Trial Documentation, Medical Writing, Non-Medical Regulatory Writing, Technical Writing, General Proofreading & Editing

Research Scientific and Technical Research

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis, Image Processing, Image Analysis, Data Visualization

Work Experience

Postdoctoral Fellow

The Ohio State University

May 2023 - Present

Postdoctoral Research fellow

Van Andel Research Institute

September 2020 - May 2023

Lab Manager

University of Pittsburgh

April 2012 - July 2015

Lab Associate

University of Miami

June 2011 - March 2012

Lab Manager

University of Pittsburgh

April 2010 - April 2011

Education

PhD (Pathology)

University of Pittsburgh

August 2015 - June 2021

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Yuan Liu, Travis B. Lear, Manish Verma, Kent Z.Q. Wang, P. Anthony Otero, Alison C. McKelvey, Sarah R. Dunn, Erin Steer, Nicholas W. Bateman, Christine Wu, et al. (2020). Chemical inhibition of FBXO7 reduces inflammation and confers neuroprotection by stabilizing the mitochondrial kinase PINK1 . JCI Insight.

Patrick D. Wilkinson, Frances Alencastro, Evan R. Delgado, Madeleine P. Leek, Matthew P. Weirich, P. Anthony Otero, Nairita Roy, Whitney K. Brown, Michael Oertel, Andrew W. Duncan(2019). Polyploid Hepatocytes Facilitate Adaptation and Regeneration to Chronic Liver Injury . The American Journal of Pathology. 189. (6). p. 1241--1255. Elsevier {BV}