Profile Details

Create Project

★★★★★

☆☆☆☆☆

USD 50 /hr

★★★★★

☆☆☆☆☆

Hire Dr. Amol S.

United States

USD 50 /hr

Protein engineer with 15+ years of academic and industry experience

Profile Summary

• Experienced leader with extensive expertise in protein engineering, antibody engineering, biosensors, and peptide discovery. Proven ability to build and lead high-performing teams by integrating directed evolution, computational modeling, automation & machine learning into biologics discovery. Passionate about developing novel biologics and collaborating across disciplines to drive innovation. • Biomolecules Discovered/Engineered: phytase, xylanase, masked-antibody, biosensors for neuromodulators and CNS drugs, cyclic peptide. Products/Processes Launched Xylanase as Frontia® Prime by Novozymes, Denmark (valued at USD $19.5B) Phytase as Natuphos® E by BASF, Germany (valued at USD $510M) Bioprocess for commercial manufacturing two APIs at Pfizer, USA (>$15M savings/year)

Subject Matter Expertise

• ☆ Biocatalysis
• ☆ Molecular Modeling
• ☆ Molecular Docking
• ☆ Molecular Biology
• ☆ Molecular Evolution
• ☆ PCR
• ☆ Molecular Cloning
• ☆ Biotechnology & Bioengineering
• ☆ Recombinant Protein Expression
• ☆ Data Analysis
• ☆ Computer Simulation/Computational Modeling
• ☆ Biomolecular Engineering
• ☆ Protein/Enzyme Engineering

Services

• Research
• Consulting

Research Feasibility Study, Technology Scouting, Fact Checking, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Work Experience

Manager/Team Lead

Pfizer

November 2022 - Present

Senior Scientist

Howard Hughes Medical Institute

June 2021 - Present

Senior Scientist

California Institute of Technology

December 2019 - June 2021

Senior Scientist

Novozymes South Asia Pvt Ltd

June 2018 - October 2019

Senior postdoctoral fellow

California Institute of Technology

February 2014 - May 2018

Postdoctoral fellow

University of California Santa Barbara

February 2012 - February 2014

Education

PhD (Biochemical Engineering)

Jacobs University Bremen gGmbH

November 2007 - December 2010

M.S. (Pharm) (Biotechnology)

National Institute of Pharmaceutical Education and Research

June 2005 - June 2007

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, et al.(2020). Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning . Cell.

Bera K, Kamajaya A, Shivange AV, Muthusamy AK, Nichols AL, Borden PM, Grant S, Jeon J, Lin E, Bishara I, et al.(2019). Biosensors Show the Pharmacokinetics of S-Ketamine in the Endoplasmic Reticulum . Frontiers in cellular neuroscience.

Kurdekar V, Giridharan S, Subbarao J, Nijaguna MB, Periasamy J, Boggaram S, Shivange AV, Sadasivam G, Padigaru M, Potluri V, et al.(2019). Structure-Guided Synthesis and Evaluation of Small-Molecule Inhibitors Targeting Protein-Protein Interactions of BRCA1 tBRCT Domain . ChemMedChem.

Shivange AV, Borden PM, Muthusamy AK, Nichols AL, Bera K, Bao H, Bishara I, Jeon J, Mulcahy MJ, Cohen B, et al.(2019). Determining the pharmacokinetics of nicotinic drugs in the endoplasmic reticulum using biosensors . The Journal of general physiology.

Shivange AV, Hoeffken HW, Haefner S, Schwaneberg U(2016). Protein consensus-based surface engineering (ProCoS): a computer-assisted method for directed protein evolution . BioTechniques.

Shivange, A.V., Roccatano, D., Schwaneberg, U.(2016). Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution . Applied Microbiology and Biotechnology. 100. (1). p. 227-242.

Shivange, A.V., Hoeffken, H.W., Haefner, S., Schwaneberg, U.(2016). Protein consensus-based surface engineering (Procos): A computer-assisted method for directed protein evolution . BioTechniques. 61. (6). p. 305-314.

Shivange AV, Roccatano D, Schwaneberg U(2015). Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution . Applied microbiology and biotechnology.

Shivange AV, Daugherty PS(2015). De novo discovery of bioactive cyclic peptides using bacterial display and flow cytometry . Methods in molecular biology (Clifton, N.J.).

Belsare KD, Ruff AJ, Martinez R, Shivange AV, Mundhada H, Holtmann D, Schrader J, Schwaneberg U(2014). P-LinK: A method for generating multicomponent cytochrome P450 fusions with variable linker length . BioTechniques.

Belsare, K.D., Joëlle Ruff, A., Martinez, R., Shivange, A.V., Mundhada, H., Holtmann, D., Schrader, J., Schwaneberg, U.(2014). P-LinK: A method for generating multicomponent cytochrome P450 fusions with variable linker length . BioTechniques. 57. (1). p. 13-20.

Shivange, A.V., Dennig, A., Schwaneberg, U.(2014). Multi-site saturation by OmniChange yields a pH- and thermally improved phytase . Journal of Biotechnology. 170. (1). p. 68-72.

Dudek, H.M., Fink, M.J., Shivange, A.V., Dennig, A., Mihovilovic, M.D., Schwaneberg, U., Fraaije, M.W.(2014). Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis . Applied Microbiology and Biotechnology. 98. (9). p. 4009-4020.

Shivange AV, Dennig A, Schwaneberg U(2013). Multi-site saturation by OmniChange yields a pH- and thermally improved phytase . Journal of biotechnology.

Dudek HM, Fink MJ, Shivange AV, Dennig A, Mihovilovic MD, Schwaneberg U, Fraaije MW(2013). Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis . Applied microbiology and biotechnology.

Jakoblinnert A, van den Wittenboer A, Shivange AV, Bocola M, Heffele L, Ansorge-Schumacher M, Schwaneberg U(2013). Design of an activity and stability improved carbonyl reductase from Candida parapsilosis . Journal of biotechnology.

Jakoblinnert A, Wachtmeister J, Schukur L, Shivange AV, Bocola M, Ansorge-Schumacher MB, Schwaneberg U(2013). Reengineered carbonyl reductase for reducing methyl-substituted cyclohexanones . Protein engineering, design & selection : PEDS.

Jakoblinnert, A., Wachtmeister, J., Schukur, L., Shivange, A.V., Bocola, M., Ansorge-Schumacher, M.B., Schwaneberg, U.(2013). Reengineered carbonyl reductase for reducing methyl-substituted cyclohexanones . Protein Engineering, Design and Selection. 26. (4). p. 291-298.

Jakoblinnert, A., van den Wittenboer, A., Shivange, A.V., Bocola, M., Heffele, L., Ansorge-Schumacher, M., Schwaneberg, U.(2013). Design of an activity and stability improved carbonyl reductase from Candida parapsilosis . Journal of Biotechnology. 165. (1). p. 52-62.

Ruff, A.J., Marienhagen, J., Verma, R., Roccatano, D., Genieser, H.-G., Niemann, P., Shivange, A.V., Schwaneberg, U.(2012). DRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method . Journal of Molecular Catalysis B: Enzymatic. 84. p. 40-47.

Shivange, A.V., Serwe, A., Dennig, A., Roccatano, D., Haefner, S., Schwaneberg, U.(2012). Directed evolution of a highly active Yersinia mollaretii phytase . Applied Microbiology and Biotechnology. 95. (2). p. 405-418.

Shivange AV, Serwe A, Dennig A, Roccatano D, Haefner S, Schwaneberg U(2011). Directed evolution of a highly active Yersinia mollaretii phytase . Applied microbiology and biotechnology.

Dennig A, Shivange AV, Marienhagen J, Schwaneberg U(2011). OmniChange: the sequence independent method for simultaneous site-saturation of five codons . PloS one.

Dennig, A., Shivange, A.V., Marienhagen, J., Schwaneberg, U.(2011). Omnichange: The sequence independent method for simultaneous site-saturation of five codons . PLoS ONE. 6. (10).

Shivange AV, Schwaneberg U, Roccatano D(2010). Conformational dynamics of active site loop in Escherichia coli phytase . Biopolymers.

Shivange, A.V., Schwaneberg, U., Roccatano, D.(2010). Conformational dynamics of active site loop in Escherichia coli phytase . Biopolymers. 93. (11). p. 994-1002.

Kaur, S., Shivange, A.V., Roy, N.(2010). Structural analysis of trypanosomal sirtuin: An insight for selective drug design . Molecular Diversity. 14. (1). p. 169-178.

Kaur S, Shivange AV, Roy N(2009). Structural analysis of trypanosomal sirtuin: an insight for selective drug design . Molecular diversity.

Shivange AV, Marienhagen J, Mundhada H, Schenk A, Schwaneberg U(2009). Advances in generating functional diversity for directed protein evolution . Current opinion in chemical biology.

Shivange, A.V., Marienhagen, J., Mundhada, H., Schenk, A., Schwaneberg, U.(2009). Advances in generating functional diversity for directed protein evolution . Current Opinion in Chemical Biology. 13. (1). p. 19-25.

Prusty D, Mehra P, Srivastava S, Shivange AV, Gupta A, Roy N, Dhar SK(2008). Nicotinamide inhibits Plasmodium falciparum Sir2 activity in vitro and parasite growth . FEMS microbiology letters.

Prusty, D., Mehra, P., Srivastava, S., Shivange, A.V., Gupta, A., Roy, N., Dhar, S.K.(2008). Nicotinamide inhibits Plasmodium falciparum Sir2 activity in vitro and parasite growth . FEMS Microbiology Letters. 282. (2). p. 266-272.

Kadam RU, Shivange AV, Roy N(2007). Escherichia coli versus Pseudomonas aeruginosa deacetylase LpxC inhibitors selectivity: surface and cavity-depth-based analysis . Journal of chemical information and modeling.

Kadam, R.U., Shivange, A.V., Roy, N.(2007). Escherichia coli versus Pseudomonas aeruginosa deacetylase LpxC inhibitors selectivity: Surface and cavity-depth-based analysis . Journal of Chemical Information and Modeling. 47. (3). p. 1215-1224.

PREPRINT

Philip M. Borden, Peng Zhang, Amol V. Shivange, Jonathan S. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novak, Masashi Tanimoto, et al. (2020). A fast genetically encoded fluorescent sensor for faithfulin vivoacetylcholine detection in mice, fish, worms and flies .

Kurdekar V, Giridharan S, Subbarao J, Nijaguna MB, Periasamy J, Boggaram S, Bharatham K, Potluri V, Shivange AV, Sadasivam G, et al.(2019). Structural features underlying the activity of benzimidazole derivatives that target phosphopeptide recognition by the tandem BRCT domain of the BRCA1 protein .

OTHER

A.V. Shivange, P.M. Borden(2019). Crystal Structure of iNicSnFR 1.0 . Worldwide Protein Data Bank

Amol V. Shivange, Ulrich Schwaneberg(2017). Recent Advances in Directed Phytase Evolution and Rational Phytase Engineering . Directed Enzyme Evolution: Advances and Applications. p. 145--172. Springer International Publishing

BOOK CHAPTER

Shivange, A.V., Schwaneberg, U.(2017). Recent advances in directed phytase evolution and rational phytase engineering . Directed Enzyme Evolution: Advances and Applications. p. 145-172.

BOOK

Shivange, A.V., Daugherty, P.S.(2015). De novo discovery of bioactive cyclic peptides using bacterial display and flow cytometry . Methods in Molecular Biology. 1248. p. 139-153.